Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Direct estimate of cocoa powder content in cakes by colorimetry and photoacoustic spectroscopy
Doka, O. ; Bicanic, D.D. ; Kulcsar, R. - \ 2014
International Journal of Thermophysics 35 (2014)12. - ISSN 0195-928X - p. 2206 - 2214.
phenolic content - theobroma-cacao - antioxidant - quantification - polyphenols - chocolate - capacity - beans - fat
Cocoa is a very important ingredient in the food industry and largely consumed worldwide. In this investigation, colorimetry and photoacoustic spectroscopy were used to directly assess the content of cocoa powder in cakes; both methods provided satisfactory results. The calibration curve was constructed using a series of home-made cakes containing varying amount of cocoa powder. Then, at a later stage, the same calibration curve was used to quantify the cocoa content of several commercially available cakes. For self-made cakes, the relationship between the PAS signal and the content of cocoa powder was linear while a quadratic dependence was obtained for the colorimetric index TeX (brightness) and total color difference
Direct measurement of thermal effusivity of foods by front configuration of the photpyroelectric method
Szafner, G. ; Bicanic, D.D. ; Kulcsár, R. ; Doka, O. - \ 2014
Journal of Food Physics 26 (2014). - ISSN 1416-3365 - p. 4 - 10.
Thermophysical properties of foods are of considerable relevance to food industry. The One among less explored thermophysical quantities is the thermal effusivity. In this paper the front variant of the photopyroelectric method was applied to determine thermal effusivity of both, fresh hen egg¿s yolk and white as well as of their blends. The amount of egg yolk added to the blend affected the thermal effusivity of egg¿s white. Thermal effusivity of mixtures containing pork meat and lard was also investigated. Addition of lard reduces significantly thermal effusivity of pork meat (the 1% increase of lard content leads to 6.93 Ws½m-2K-1 drop of thermal effusivity).
Colorimetry and photoacoustic spectroscopy as suitable tools for direct determination of cocoa powder in confectionary products
Doka, O. ; Pragai, E. ; Bicanic, D.D. ; Kulcsar, R. - \ 2013
European Food Research and Technology 236 (2013)6. - ISSN 1438-2377 - p. 963 - 968.
phenolic content - theobroma-cacao - powders - antioxidant - quantification - polyphenols - mixtures - capacity - samples - beans
Laser photoacoustic spectroscopy (PAS) and colorimetry have been used to rapidly and accurately determine the content of fat-free cocoa solids in dark chocolates. Both techniques are inexpensive and require only a one-time calibration step versus a method capable of absolute concentration measurement (for example HPLC). Once the response of PAS and/or colorimetry has been obtained, the content of fat-free cocoa solids in dark chocolates can be determined directly (i.e. without any sample preparation including the process of extraction) from the calibration curves and the experimentally measured microphone signal (in PAS studies) and colorimetric indices L* and ¿E* (in colorimetric investigations). Both colorimetric indices and PA signals correlate positively with the content of fat-free cocoa solids. The correlation is highly linear over a wide concentration range (25–50 %).
Dissecting insect development : baculovirus-mediated gene silencing in insects
Hajós, J.P. ; Vermunt, A.W.M. ; Zuidema, D. ; Kulcsár, P. ; Varjas, L. ; Kort, C.A.D. de; Závodszky, P. ; Vlak, J.M. - \ 1999
Insect Molecular Biology 8 (1999)4. - ISSN 0962-1075 - p. 539 - 544.
A novel concept applying baculovirus-mediated gene silencing to study insect gene function and regulation is described in this paper. A recombinant baculovirus, Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), was constructed with the juvenile hormone esterase (JHE) gene from the tobacco budworm Heliothis virescens in the antisense orientation, driven by the viral p10 promoter. Infection with this recombinant greatly reduced the haemolymph JHE level and resulted in aberrant morphogenesis of final-instar H. virescens larvae. The body organization remained larval, although the cuticle became hard and brown, similar to pupal cuticle. These results demonstrated that baculovirus-mediated gene silencing can be accomplished and utilized to dissect insect development and to design a new class of baculovirus insecticides.
Dissecting insect development : baculovirus-mediated gene silencing in insects
Hajós, J.P. ; Vermunt, A.M.W. ; Zuidema, D. ; Kulcsár, P. ; Varjas, L. ; Kort, C.A.D. de; Zavodszky, P. ; Vlak, J.M. - \ 1999
In: 32nd Annual Meeting of the Society for Invertebrate Pathology (SIP) : 23 - 27 August 1999, Irvine, California, USA : book of abstracts / [by the] University of California, 1999. - [S.l.] : [s.n.], 1999 - p. 42 - 43.
Recombination of baculovirus DNA following lipofection in insect larvae.
Hajos, J.P. ; Zuidema, D. ; Kulcsar, P. ; Heldens, J.G.M. ; Zavodszky, P. ; Vlak, J.M. - \ 1998
Archives of Virology 143 (1998). - ISSN 0304-8608 - p. 2045 - 2050.
Spodoptera exigua multicapsid nucleopolyhedrovirus deletion mutants generated in cell culture lack virulence in vivo.
Heldens, J.G.M. ; Strien, E.A. van; Leisy, D.J. ; Feldmann, A.M. ; Muñoz, D. ; Kulcsar, P. ; Zuidema, D. ; Goldbach, R.W. ; Vlak, J.M. - \ 1996
Journal of General Virology 77 (1996). - ISSN 0022-1317 - p. 3127 - 3134.
The ribonucleotide reductase large subunit of Spodoptera exigua nuclear polyhedrosis virus: a possible virulence gene.
Strien, E.A. van; Zuidema, D. ; Hu, Z. ; Kulcsar, P. ; Goldbach, R.W. ; Vlak, J.M. - \ 1994
In: 6th Int. Coll. Invertebrate pathology and microbial control, Montpellier, France - p. 165 - 165.
Structural organization of a 17 kb fragment of Spodoptera exigua nuclear polyhedrosis virus.
Strien, E.A. van; Zuidema, D. ; Gerrits, H. ; Kulcsar, P. ; Oers, M.M. van; Vlak, J.M. - \ 1993
In: Abstract 9th Int. Congr. Virology, Glasgow, UK - p. 133 - 133.
Structural organization of a 17 kb fragment of Spodoptera exigua nuclear polyhedrosis virus.
Strien, E.A. van; Gerrits, H. ; Kulcsar, P. ; Feldmann, A.M. ; Zuidema, D. ; Vlak, J.M. - \ 1993
In: Abstract 12th Ann. Meeting Am. Soc. Virology, Davis, Ca, USA (1993) 48. Ook: Abstr. PhD Summerschool Molecular diversity in pest and disease organisms, WAU - p. 34 - 34.
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