Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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    Commonalities and Differences in the Transcriptional Response of the Model Fungus Saccharomyces cerevisiae to Different Commercial Graphene Oxide Materials
    Laguna-Teno, Felix ; Suarez-Diez, Maria ; Tamayo-Ramos, Juan Antonio - \ 2020
    Frontiers in Microbiology 11 (2020). - ISSN 1664-302X
    biological response - chelating agent - commercial graphene oxide - differential expression - RNA isolation - Saccharomyces cerevisiae - transcriptomics

    Graphene oxide has become a very appealing nanomaterial during the last years for many different applications, but its possible impact in different biological systems remains unclear. Here, an assessment to understand the toxicity of different commercial graphene oxide nanomaterials on the unicellular fungal model organism Saccharomyces cerevisiae was performed. For this task, an RNA purification protocol was optimized to avoid the high nucleic acid absorption capacity of graphene oxide. The developed protocol is based on a sorbitol gradient separation process for the isolation of adequate ribonucleic acid levels (in concentration and purity) from yeast cultures exposed to the carbon derived nanomaterial. To pinpoint potential toxicity mechanisms and pathways, the transcriptome of S. cerevisiae exposed to 160 mg L–1 of monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) was studied and compared. Both graphene oxide products induced expression changes in a common group of genes (104), many of them related to iron homeostasis, starvation and stress response, amino acid metabolism and formate catabolism. Also, a high number of genes were only differentially expressed in either GO (236) or GOC (1077) exposures, indicating that different commercial products can induce specific changes in the physiological state of the fungus.

    Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets
    Suarez-Diez, Maria ; Porras, Santiago ; Laguna-Teno, Felix ; Schaap, Peter J. ; Tamayo-Ramos, Juan A. - \ 2020
    Scientific Reports 10 (2020)1. - ISSN 2045-2322

    Graphene nanomaterials have attracted a great interest during the last years for different applications, but their possible impact on different biological systems remains unclear. Here, an assessment to understand the toxicity of commercial polycarboxylate functionalized graphene nanoplatelets (GN) on the unicellular fungal model Saccharomyces cerevisiae was performed. While cell proliferation was not negatively affected even in the presence of 800 mg L−1 of the nanomaterial for 24 hours, oxidative stress was induced at a lower concentration (160 mg L−1), after short exposure periods (2 and 4 hours). No DNA damage was observed under a comet assay analysis under the studied conditions. In addition, to pinpoint the molecular mechanisms behind the early oxidative damage induced by GN and to identify possible toxicity pathways, the transcriptome of S. cerevisiae exposed to 160 and 800 mg L−1 of GN was studied. Both GN concentrations induced expression changes in a common group of genes (337), many of them related to the fungal response to reduce the nanoparticles toxicity and to maintain cell homeostasis. Also, a high number of genes were only differentially expressed in the GN800 condition (3254), indicating that high GN concentrations can induce severe changes in the physiological state of the yeast.

    Bioturbation and erosion rates along the soil-hillslope conveyor belt, part 2: Quantification using an analytical solution of the diffusion–advection equation
    Román-Sánchez, Andrea ; Laguna, Ana ; Reimann, Tony ; Giráldez, Juan Vicente ; Peña, Adolfo ; Vanwalleghem, Tom - \ 2019
    Earth Surface Processes and Landforms 44 (2019)10. - ISSN 0197-9337 - p. 2066 - 2080.
    bioturbation - critical zone - deposition - diffusivity - erosion - feldspar luminescence dating - sensitivity and uncertainty - soil formation

    Particles on soil-mantled hillslopes are subject to downslope transport by erosion processes and vertical mixing by bioturbation. Both are key processes for understanding landscape evolution and soil formation, and affect the functioning of the critical zone. We show here how the depth–age information, derived from feldspar-based single grain post-infrared infrared stimulated luminescence (pIRIR), can be used to simultaneously quantify erosion and bioturbation processes along a hillslope. In this study, we propose, for the first time, an analytical solution for the diffusion–advection equation to calculate the diffusivity constant and erosion–deposition rates. We have fitted this model to age–depth data derived from 15 soil samples from four soil profiles along a catena located under natural grassland in the Santa Clotilde Critical Zone Observatory, in the south of Spain. A global sensitivity analysis was used to assess the relative importance of each model parameter in the output. Finally, the posterior probability density functions were calculated to evaluate the uncertainty in the model parameter estimates. The results show that the diffusivity constant at the surface varies from 11.4 to 81.9 mm2 a-1 for the hilltop and hill-base profile, respectively, and between 7.4 and 64.8 mm2 a-1 at 50 cm depth. The uncertainty in the estimation of the erosion–deposition rates was found to be too high to make a reliable estimate, probably because erosion–deposition processes are much slower than bioturbation processes in this environment. This is confirmed by a global sensitivity analysis that shows how the most important parameters controlling the age–depth structure in this environment are the diffusivity constant and regolith depth. Finally, we have found a good agreement between the soil reworking rates proposed by earlier studies, considering only particle age and depth, and the estimated diffusivity constants. The soil reworking rates are effective rates, corrected for the proportion of particles actually participating in the process.

    An assessment of policies affecting Sustainable Soil Management in Europe and selected member states
    Turpin, Nadine ; Berge, Hein ten; Grignani, Carlo ; Guzmán, Gema ; Vanderlinden, Karl ; Steinmann, Horst-Henning ; Siebielec, Grzegorz ; Spiegel, Adelheid ; Perret, Eric ; Ruysschaert, Greet ; Laguna, Ana ; Giráldez, Juan Vicente ; Werner, Magdalena ; Raschke, Isabell ; Zavattaro, Laura ; Costamagna, Chiara ; Schlatter, Norman ; Berthold, Helen ; Sandén, Taru ; Baumgarten, Andreas - \ 2017
    Land Use Policy 66 (2017). - ISSN 0264-8377 - p. 241 - 249.
    This paper analyses soils-related policies in Europe and in selected member states and regions. Our approach breaks down policy packages at European, national and regional levels into strategic objectives, operational objectives, policy measures and expected impacts, and assesses the relationships between these elements and soil stakes. Four major policy packages, both at EU and national level (CAP-I, RDP, Environment, national initiatives) were analysed. A numerical scale was developed to quantify the level of “embeddedness” of soil stakes in these policy packages. We found that countries better embed soil stakes into their policies when they also put more efforts on environmental innovation. In turn, countries with a high embeddedness level, with high trust in European institutions and that make more efforts towards renewable energy, tend to propose a wider variety of management practices to farmers for dealing with soil stakes.
    Attitude toward living kidney donation : Differences between students from two spanish universities
    Martínez-Alarcón, L. ; Ramis, G. ; Gómez-Laguna, J. ; Quereda, J.J. ; Herrero-Medrano, J.M. ; Mrowiec, A. ; Mendonça, L. ; López-Navas, A. ; Ríos, A. - \ 2015
    Transplantation Proceedings 47 (2015)1. - ISSN 0041-1345 - p. 10 - 12.

    Introduction Due to the current deficit of organs for transplantation, living kidney related donations (LKRD) should be promoted. Veterinarians often hold decision-making positions in the public health care system, and therefore can influence public opinion about organ donation. The objective was to analyze the attitude of Spanish veterinary students toward LKRD because they may influence public opinion in the future, and to determine the factors that condition it. Materials and Methods The study was carried out among fifth-year veterinary science students from 2 southern and southeastern Spanish universities. The students' attitude toward LKRD was assessed using a psychosocial, anonymous, self-administered questionnaire. Statistics used were χ2 test and Student t test. Results Data from the southern Spain university included a response rate of 87%. The survey showed that 94% of respondents would donate a kidney to a relative who needed it. This attitude toward LKRD was more favorable in women (P

    List of drivers and barriers governing soil management by farmers, including cost aspects : D4.434
    Pronk, A.A. ; Bijttebier, J. ; Berge, H.F.M. ten; Ruysschaert, G. ; Hijbeek, R. ; Rijk, B. ; Werner, J.M. ; Raschke, I. ; Steinmann, H.H. ; Zylowska, K. ; Schlatter, N. ; Guzman, G. ; Syp, A. ; Bechini, L. ; Turpin, N. ; Guiffant, N. ; Perret, E. ; Mauhé, N. ; Toqué, C. ; Zavattaro, L. ; Costamagna, C. ; Grignani, C. ; Lehninen, T. ; Baumgarten, A. ; Spiegel, H. ; Portero, A. ; Walleghem, T. Van; Pedrera, A. ; Laguna, A. ; Vanderlinden, K. ; Giráldez, V. ; Verhagen, A. - \ 2015
    {Wageningen] : Stichting Dienst Landbouwkundig Onderzoek (DLO) - 180
    Farmers review of Best Management Practices: drivers and barriers as seen by adopters and non-adopters : Report D4.422
    Bijttebier, J. ; Ruysschaert, G. ; Hijbeek, R. ; Rijk, B. ; Werner, M. ; Raschke, I. ; Steinmann, H.H. ; Zylowska, K. ; Pronk, A. ; Schlatter, N. ; Guzmán, G. ; Syp, A. ; Bechini, L. ; Turpin, N. ; Guiffant, N. ; Perret, E. ; Mauhé, N. ; Toqué, C. ; Zavattaro, L. ; Costamagna, C. ; Grignani, C. ; Lehninen, T. ; Baumgarten, A. ; Spiegel, H. ; Portero, A. ; Walleghem, T. Van; Pedrera, A. ; Laguna, A. ; Vanderlinden, K. ; Giráldez, V. - \ 2015
    CATCH-C (Report D4.422 and D4.443 ) - 171 p.
    Assessing farmers’ intention to adopt best management practices across eight European countries
    First report of Pseudocercospora jahnii in the Philippines
    Acabal Jr, B.D. ; Groenewald, J.Z. ; Crous, P.W. ; Cumagun, C.J.R. - \ 2014
    Australasian Plant Disease Notes 9 (2014). - ISSN 1833-928X
    Young and mature leaves with necrotic spots of Tabebuia pallida were collected in field experimental plots in Mandaue City, Cebu, and Laguna in the Philippines. The leaf spots were colonised by a cercosporoid fungus identified as Pseudocercospora jahnii, which is a first report of this pathogen from the Philippines.
    Determination of traffic control tables by HPC
    Hendrix, E.M.T. ; Tabik, S. ; Haijema, R. - \ 2011
    In: Actas de las XXII Jornadas de Paralelismo, La Laguna, Spain, 7 - 9 September, 2011. - - p. 131 - 134.
    Mallas y flujos : acción colectiva, cambio social, quinua y desarrollo regional indígena en los Andes Bolivianos
    Laguna, P. - \ 2011
    Wageningen University. Promotor(en): Leontine Visser, co-promotor(en): Alberto Arce. - [S.l.] : S.n. - ISBN 9789085859604 - 522
    chenopodium quinoa - inheemse volkeren - producentengroepen - antropologie - sociale verandering - modernisering - economische ontwikkeling - sociale ontwikkeling - coöperatieve verenigingen - bolivia - andes - ontwikkeling - zuid-amerika - chenopodium quinoa - indigenous people - producer groups - anthropology - social change - modernization - economic development - social development - cooperative societies - bolivia - andes - development - south america

    This thesis studies collective action and social change in indigenous rural organisations (IRO) in the Bolivian Andes. I focus on the effects and importance that these organisations have in the historical process of regional development as social spaces that encapsulate different projects of social, political and economic modernity. I reconstruct the practices and situations that turn rural indigenous organisations into significant spaces in which individuals and groups of people put into practice their life projects and their aspirations of modernity. The main question of this thesis is: what are indigenous rural organisations in the Bolivian Andes and what are their contributions to regional development?

    To answer this question, I argue that we need to leave aside social constructivism and rational action present in current studies of indigenous rural organisations in the Andes that use the concept social capital. These organisations are not essences, totalities, nor are they are stable. Also, they are a more complex process than mere rational and technocratic action. IRO are contextual and situational spaces of social life that contain significant elements or objects, which are material and immaterial. These spaces are heterogeneities of humans and objects united by shared significant objects that are emergent, original and intensive. In this sense this organisations represent meshworks that interweave the changeable relationships between entities (humans and objects) and practices, and encompass the possibility of social change. These meshworks have different dimensions (economical, social, cultural, political). In each one of those, the flow of practices, interactions and experiences of individuals and groups of individuals simultaneously unify and break meaning, identity, affect, materiality and also regulation.

    I study three kindsof indigenous rural organisations fromthe Perisalar (the Bolivian Southern highlands): communities which are based on kinship relationships, ayllus which are ethnic groups and quinoa producer organisations. Communities are social spaces that contain significant elements of modernity, such as the desire for access to State education and to enjoy citizens’ rights, the wish for agricultural machinery and to produce for the global market, the diversity of livelihoods and the affirmation of racial and class identity. Ayllus are made by community assemblages and many comunarios belong to quinoa Producer Organisations. In this sense ayllus and producer organisations are important social spaces as they contain significant elements present in the communities. I present the social life of IRO starting from the intersection of local development practices and experiences with other social spaces: the market, migratory destinations, education, social movements and institutional intervention. In order to better understand the effects of social change and IRO, I chose a long-term historical vision, considering the emerging effects of the intersection of local and external practices and experiences, before and during the quinoa commoditisation process.

    The study concludes that IRO in the Bolivian Andes, are meshworks made by vibrant humans and objects with social vitality and intensity. They have the capacity to actualise significant elements of an economic, social, cultural and political character, in interaction with the Nation-State and the global market. These organisations increase through global market the vibrant character of significant elements such as quinoa, and by their recognition by the State they provide semi-autonomy to their members, and a space to make recognised their citizenship and their trade union, racial and class identities, and to locally redesign the State. Memory, identity and affect reveal the potential of IRO in repositioning past reminiscences and ancestral properties, and at the same time claim for a future that does not contain the same substance of that which is “the Andean”, “the Aymara” or “the Quechua”, rather incorporates new elements that lead to multiple “(neo)Andeans”, “(neo)Aymaras” and “(neo)Quechuas” forms, present in each and every one of the partial connections.

    These organisations contain a variety of symbols, discourses and practices that correspond to heterogeneous knowledge and forms of socialisation and thinking of modernity that sometimes result in tension, fissure and conflict without however being fragmented. That is why structuralism, institutionalism and rationalism partially explain the agency in ambiguous and eclectic social spacessuch areIRO, whose limitsare constantly redefined by the flow of experience of its members. Development through these organisations is a social process, experiential and unpredictable, reflexive and corporeal, cognitive and performative, that contains both cohesion and tear. For understanding IRO contribution to rural development we must describe the relational and the imaginative in the wishes and processes of social change and regional developmentand grasp the relevance of its individual members’ experiences and practices in the creation of social ties. Methodologically this leads us to dissolve analytical categories and to follow and observe individuals past and present practices and their intersections with other individuals, groups, structures and significant objects. Our study underlines the significance of human-object relation as a starting point for generating new analytical frameworks in indigenous Andean organizations.

    Del altiplano sur boliviano hasta el mercado global: coordinación y estructuras de gobernancia en la cadena de valor de la quinua orgánica y del comercio justo
    Laguna, P. ; Cáceres, Z. ; Carimetrand, A. - \ 2006
    Agroalimentaria 12 (2006)22. - ISSN 1316-0354 - p. 65 - 76.
    This article aims to describe and to analyze the recent development of real organic quinoa global value chain, linked to consumer demand in specific quality food markets. Taking into account small producers¿ organizations and local and multinational private companies, the authors consider the evolution of forms of coordination and governance structure linked to organic and fair trade quality, in particular the creation of peasants economic organization, contract farming, and European distributor subsidiaries. The main tool for this analysis is the French Conventions Theory and the Global Value Chain approach
    Del Altiplano Sur boliviano hasta el mercado global: coordinación, regulación y calidad en el mercado en la cadena de valor de la quinua
    Laguna, P. ; Cáceres, Z. ; Carimetrand, A. - \ 2006
    In: Agroindustria Rural y Territorio (tomo1): Los desafíos de los sistemas agroalimentarios localizados / Álvarez, A, Boucher, F, Mexico : Universidad Autónoma del Estado de México - ISBN 9789688359792 - p. 375 - 407.
    Fitosociología y fitodiversidad de la Laguna Quila, Parque Nacional Lagunas de Zempoala, México
    Islebe, G. ; Almeida-Lenero, L. ; Cleef, A.M. ; Weijden, R. ter - \ 2003
    Acta Botanica Mexicana 65 (2003). - ISSN 0187-7151 - p. 61 - 82.
    Participatory research with user groups : key issues and challenges
    Niehof, A. ; Castillo, G.T. ; Fuglie, K.O. - \ 2003
    In: From Cultivators to Consumers : participatory research with various user groups Los Baños, Laguna, Philippines : CIP-UPWARD - ISBN 9789716140279 - p. 1 - 16.
    La cadena global de la Quinua : un reto para la Asociación Nacional de Productores de Quinua
    Laguna, P. - \ 2002
    In: La gestión economica-ambiental en las cadenas globales de mercancías en Bolivia / Romero Padilla, C., Peluppessy, E., Cochabamba : Universidad Mayor de San Simon - ISBN 9789990501865 - p. 89 - 195.
    Heterogeneidad, cultura, impacto, acción individual y colectiva : por un nuevo enfoque en el estudio de las organizaciones económicas campesinas bolivianas
    Laguna, P. - \ 2002
    T'inkasos : revista boliviana de ciencias sociales 12 (2002). - p. 99 - 117.
    Competitividad, externalidas e internalidades, un reto para las organizaciones económicas campesinas : La inserción de la Associación Nacional de Productores de Quinua en el mercado mundial de la quinua
    Laguna, P. - \ 2002
    Debate Agrario 34 (2002). - ISSN 1017-9011 - p. 95 - 169.
    Rootcrop Agriculture in the Philippines : The Quest for Food Security and Livelihood
    Pardales jr., J.R. ; Roa, J.R. - \ 2002
    In: Secondary Farmers of Secondary Crops? Women and Rootcrop Livelihood in the Philippines - p. 3 - 11.
    División Bryophyta
    Losada-Lima, A. ; Dirkse, G.M. ; Rodríguez-NúAez, S. - \ 2001
    In: Lista de especies silvestres de Canarias (hongos, plantas y animales terrestres) 2001. La Laguna (Tenerife, Spain), Consejería de Política Territorial y Medio Ambiente del Goberno de Canarias / Izquierdo, I., Martín, J.L., Zurita, N., Arechavaleta, M., - p. 88 - 97.
    flora - bryologie - taxonomie - vegetatie - Spanje - Canarische Eilanden
    Flora brilógica de la ciudad de La Laguna (Tenerife, Islas Canarias)
    Losada-Lima, A. ; Dirkse, G.M. ; Abella Marrero, M. ; Betrán Tejera, E. - \ 2001
    Boletín de la Sociedad Española de Briología 18/19 (2001). - p. 115 - 119.
    bryologie - mossen - vegetatie - Spanje - Canarische Eilanden
    The impact of quinea export on peasant's livelihoods of Bolivian highlands
    Laguna, P. - \ 2000
    In: X World Congres of the International Rural Sociology Association, Rio de Janeiro : July 30-August 5, 2000. - Brazil : [s.n.], 2000. - Published on CD-rom
    Heterogeneidad, cultura, impacto, acción individual y colectiva : por un nuevo enfoque en el estudio de las organizaciones económicas campesinas bolivianes
    Laguna, P. - \ 1999
    In: Sociólogos en el umbral del Siglo XXI : II Congreso Nacional de Sociologia, La Paz, 1999 / G. Danilo Paz, R. Sandoval, S. León, P. Romero, M.I. Pérez (eds). - [S.l.] : Colegio de Sociólogos de Bolivia, 1999. - ISBN 84-89891-53-2 - p. 211 - 233.
    Population dynamics of rice leaffolders(Lepidoptera: Pyralidae) and their natural enemies in irrigated rice in the Philippines
    Kraker, J. de; Huis, A. van; Heong, K.L. ; Lenteren, J.C. van; Rabbinge, R. - \ 1999
    Bulletin of Entomological Research 89 (1999)5. - ISSN 0007-4853 - p. 411 - 421.
    Populations of rice leaffolders and their natural enemies were studied in eight crops of irrigated rice in Laguna Province, the Philippines. The rice leaffolder complex consisted of three species: Cnaphalocrocis medinalis (Guenée), Marasmia patnalis Bradley and M. exigua Butler. Leaffolder population dynamics were characterized by an egg peak at maximum tillering and a broad larval peak around booting stage. Peak densities ranged from 0.2 to 2.0 larvae per hill. Most larvae originated from immigrant moths and there was no substantial second generation. The seasonal percentage egg parasitism by Trichogramma sp. ranged from 0 to 27°and percentage larval parasitism from 14 to 56ÐThe braconid Macrocentrus philippinensis Ashmead was the most commonly reared larval parasitoid. Forty natural enemy taxa that may attack rice leaffolders were identified from suction and sweepnet samples: 24 predator taxa and 16 parasitoid taxa. The estimated survival rates from leaffolder egg to larval stages and between larval stages showed large variation between rice crops, but were not clearly correlated with observed levels of parasitism, natural enemy abundance, or natural enemy to leaffolder ratios. It is suggested that the generally low densities of rice leaffolders in Philippine transplanted rice are caused by their ovipositional preference for crops at the maximum tillering stage, allowing for only one generation, and by high immature mortality caused by the abundant and diverse complex of natural enemies.
    Egg mortality of rice leaffolders Cnaphalocrocis medinalis and Marasmia patnalis in irrigated rice fields
    Kraker, J. de; Huis, A. van; Lenteren, J.C. van; Heong, K.L. ; Rabbinge, R. - \ 1999
    Biocontrol Science and Technology 44 (1999)4. - ISSN 0958-3157 - p. 451 - 473.
    Egg mortality of rice leaf folders Cnaphalocrocis medinalisand Marasmia patnalis was studied in unsprayed irrigated rice fields in Laguna Province, the Philippines. Mortality was assessed by field exposure of laboratory-laid eggs for two days and by monitoring of field-laid eggs. Egg disappearance, the major mortality factor, was low in the first four weeks after transplanting and then increased. Egg parasitism by Trichogrammajaponicum was highest at the start of the crop and decreased to a low level towards crop maturity. Non-hatching of eggs was of minor importance. Over the total duration of the egg stage, the average disappearance of exposed laboratory-laid eggs was40%, and of field-laid eggs 46%. Egg mortality due to parasitism averaged 15% and 18%, respectively. The potential impact of egg parasitism is probably partly obscured by the disappearance of parasitized eggs. Mortality rates were highly variable between egg cohorts, but with multiple regression analysis several factors were identified that statistically explained a significant part of this variation. The results suggest that the predatory crickets Metiochevittaticollis and Anaxipha longipennis play a major role in egg disappearance, and that egg parasitism is positively dependent on the overall density of host eggs of Trichogramma in the field.
    Impact of UV-radiation on plankton in an arctic freshwater lagoon (Brandal Laguna, Spitsbergen).
    Donk, E. van; Lange, H.J. de; Faafeng, B.A. - \ 1998
    In: SIL XXVII Congress, Dublin, Ireland (1998)
    Users' Perspectives: Literature Review on the Development of a Concept. UPWARD,.
    Hardon-Baars, A. - \ 1997
    Unknown Publisher - 46 p.
    User/use-oriented approaches to technology assessment and innovation: some lessons for marketing research.
    Hardon-Baars, A. - \ 1996
    In: Into action research: partnership in Asian rootcrop research and development - p. 203 - 216.
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