Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

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Engineering Cowpea Mosaic Virus RNA-2 into a vector to express heterologous proteins in plants
Kodetham Gopinath, ; Wellink, J. ; Porta, C. ; Taylor, K.M. ; Lomonossoff, G.P. ; Kammen, A. van - \ 2000
Virology 267 (2000). - ISSN 0042-6822 - p. 159 - 173.
series of new cowpea mosaic virus (CPMV) RNA-2-based expression vectors were designed. The jellyfish green fluorescent protein (GFP) was introduced between the movement protein (MP) and the large (L) coat protein or downstream of the small (S) coat protein. Release of the GFP inserted between the MP and L proteins was achieved by creating artificial processing sites each side of the insert, either by duplicating the MP-L cleavage site or by introducing a sequence encoding the foot-and-mouth disease virus (FMDV) 2A catalytic peptide. Eight amino acids derived from the C-terminus of the MP and 14–19 amino acids from the N-terminus of the L coat protein were necessary for efficient processing of the artificial Gln/Met sites. Insertion of the FMDV 2A sequence at the C-terminus of the GFP resulted in a genetically stable construct, which produced particles containing about 10 GFP-2A-L fusion proteins. Immunocapture experiments indicated that some of the GFP is present on the virion surface. Direct fusion of GFP to the C-terminus of the S coat protein resulted in a virus which was barely viable. However, when the sequence of GFP was linked to the C-terminus by an active FMDV 2A sequence, a highly infectious construct was obtained.
Studies on hybrid comoviruses reveal the importance of three-dimensional structure for processing of the viral coat proteins and show that the specificity of cleavage is greater in trans than in cis
Clark, A.J. ; Bertens, P. ; Wellink, J. ; Shanks, M. ; Lomonossoff, G.P. - \ 1999
Virology 263 (1999)1. - ISSN 0042-6822 - p. 184 - 194.
Homologus sequences in non-structural proteins from cowpea mosaic virus and picornaviruses
Franssen, H. ; Leunissen, J. ; Goldbach, R.W. ; Lomonossoff, G. ; Zimmern, D. - \ 1984
The EMBO Journal 3 (1984). - ISSN 0261-4189 - p. 855 - 861.
Cowpea mosaic virus VPg: sequencing of radiochemically modified protein allows mapping of the gene on B RNA
Zabel, P. ; Moerman, M. ; Lomonossoff, G. ; Shanks, M. ; Beyreuther, K. - \ 1984
In: Abstract EMBO Workshop 'Plant viruses, satellites and viroids: genome structure, expression and replication' Strasbourg 17-19 July 1984
Cowpea mosaic virus VPg: sequencing of radiochemically modified protein allows mapping of the gene on B RNA
Zabel, P. ; Moerman, M. ; Lomonossoff, G. ; Shanks, M. ; Beyreuther, K. - \ 1984
The EMBO Journal 3 (1984). - ISSN 0261-4189 - p. 1629 - 1634.
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