Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Effects of structural modifications on some physical characteristics of exopolysaccharides from Lactococcus lactis
Tuinier, R. ; Casteren, W.H.M. van; Looijesteijn, P.J. ; Schols, H.A. ; Voragen, A.G.J. ; Zoon, P. - \ 2001
Biopolymers 59 (2001). - ISSN 0006-3525 - p. 160 - 166.
The relationship between the primary structure and the chain stiffness of exopolysaccharides (EPSs) and modified EPSs produced by two strains of Lactococcus lactis subsp. cremoris was investigated. The molar mass and radius of gyration of these exopolysaccharides were analyzed by multiangle static light scattering after size-exclusion chromatography. From these results and the chemical structure of the repeating units of the investigated EPSs, the Kuhn lengths could be calculated. We found that the initial Kuhn lengths of the two native EPSs are similar. Modification of the EPSs by removing parts of the side groups resulted in a decrease in both the absolute value and the normalized value of the Kuhn length. It is therefore concluded that partial removal of the side groups of these polysaccharides could make them less efficient as thickeners if no specific interaction with other components occurs
Physiological function of exopolysaccharides produced by Lactococcus lactis
Looijesteijn, P.J. ; Trapet, L. ; Vries, E. de; Abee, T. ; Hugenholtz, J. - \ 2001
International Journal of Food Microbiology 64 (2001). - ISSN 0168-1605 - p. 71 - 80.
Influence of different substrate limitations on the yield, composition and molecular mass of exopolysaccharides produced by Lactococcus lactis subsp. cremoris in continuous cultures
Looijesteijn, P.J. ; Casteren, W.H.M. van; Tuinier, R. ; Doeswijk-Voragen, C.H.L. ; Hugenholtz, J. - \ 2000
Journal of Applied Microbiology 89 (2000). - ISSN 1364-5072 - p. 116 - 122.
Physiology of exopolysaccharide biosynthesis by Lactococcus lactis
Looijesteijn, E. - \ 2000
Agricultural University. Promotor(en): J.A.M. de Bont; J. Hugenholtz. - S.l. : S.n. - ISBN 9789058082862 - 197
lactococcus lactis - industriële microbiologie - biosynthese - biochemie - fysiologie - oligosacchariden - koolhydraten - melkzuurbacteriën - lactococcus lactis - industrial microbiology - biosynthesis - biochemistry - physiology - oligosaccharides - carbohydrates - lactic acid bacteria

Several lactic acid bacteria (LAB) produce exopolysaccharides (EPS). EPSs produced by LAB are a potential source of natural additives and because LAB are food grade organisms, these EPSs can also be produced in situ . The amount of EPS in milk fermented with strain NIZO B40, which produces an anionic EPS composed of glucose, rhamnose, galactose and phosphate, is very low. This relatively low concentration could be increased by optimising the culture conditions and medium composition. Using pH-controlled fermentations and a chemically defined medium, the total EPS production was highest at pH 5.8 and 25 °C. Glucose was demonstrated to be the most efficient sugar source for EPS production by L. lactis NIZO B40. With fructose as the sugar source only a minor amount of EPS was produced. The intracellular levels of sugar nucleotides, the EPS precursors, were much lower in fructose- than in glucose-grown cultures. The activity of the enzymes involved in the biosynthesis of the sugar nucleotides were however unaffected by the source of sugar but the activity of fructose-1,6-bisphosphatase (FBPase) was very low. FBPase catalyses the conversion of fructose-1,6-diphosphate into fructose-6-phosphate, an essential step for the biosynthesis of sugar nucleotides from fructose but not from glucose. Overexpression of the fbp gene resulted in increased EPS synthesis on fructose.

Most culture conditions influenced growth as well as EPS formation and EPS synthesis itself was also influenced by the growth rate. EPS production by strain NIZO B40 starts at the exponential growth phase but continues during the stationary phase in batch cultures, indicating that EPS biosynthesis and growth are not strictly coupled. Indeed we found that non-growing cultures were still able to produce EPS, making it possible to study the influence of different culture conditions on EPS biosynthesis independent of growth.

The amounts of EPS produced by L. lactis NIZO B40 and NIZO B891 were comparable under glucose and leucine limitation. The efficiency of EPS production, the quantity of EPS produced per quantity of glucose consumed, was however much higher under conditions of glucose limitation. The production of phosphorylated B40 EPS and of unphosphorylated B891 EPS was strongly reduced under conditions of phosphate limitation. The sugar composition of both B40 and B891 EPS and the phosphate content of B40 EPS were unaffected by the type of limitation but surprisingly, glucose limitation resulted in the production of EPSs with strongly reduced molecular masses.

Anionic B40 EPS in suspension and a cell-associated layer of this EPS protected the bacteria against toxic copper ions and nisin, probably due to charge interactions. Furthermore, cell-associated EPS resulted in a decrease in the sensitivity of the bacteria to bacteriophages and lysozyme, most likely by masking the targets for the phages and the enzyme. The protection of EPS against nisin and bacteriophages could be a competitive advantage in mixed strain dairy starter cultures. Unfortunately, the EPS yields were not increased in the presence of copper, bacteriophages, nisin or lysozyme.

Tuinbouwtechniek in Japan : verslag van een studiereis
Looijesteijn, F.X.C. - \ 1995
Wageningen : IMAG-DLO - 32
tuinbouw - landbouwtechniek - excursies - japan - horticulture - agricultural engineering - field trips
Techniek elders: high tech moet Japanse land- en tuinbouw redden. (11)
Looijesteijn, F.X.C. - \ 1995
Landbouwmechanisatie 46 (1995)10. - ISSN 0023-7795 - p. 62 - 63.
agronomie - automatisering - uitstrooien - oogsten - japan - verwerking - productie - robots - geïntegreerde bedrijfssystemen - agronomy - automation - broadcasting - harvesting - processing - production - integrated farming systems
In het tweede artikel over dit onderwerp komen aan de orde het strooien van kunstmest, de ontwikkeling van industriele produktiesystemen en het robotiseren van de oogst van (vrucht)groenten.
Techniek elders: high tech moet Japanse land- en tuinbouw redden. (1)
Looijesteijn, F.X.C. - \ 1995
Landbouwmechanisatie 46 (1995)9. - ISSN 0023-7795 - p. 70 - 71.
agronomie - modernisering - innovaties - landbouwtechniek - mechanisatie - automatisering - verwerking - productie - robots - verbetering - japan - agronomy - modernization - innovations - agricultural engineering - mechanization - automation - processing - production - improvement
Door het stimuleren van mechanisatie, automatiseering en robotisering, probeert de Japanse overheid het werk voor de steeds ouder wordende ondernemers te verlichten en de produktiekosten laag te houden
Japan: hoogwaardige techniek tegenover vergrijzing
Looijesteijn, F.X.C. - \ 1995
Groenten en Fruit. Vakdeel glasgroenten 5 (1995)20. - ISSN 1380-3573 - p. 16 - 19.
automation - horticulture - japan - mechanization - greenhouse horticulture - automatisering - tuinbouw - mechanisatie - glastuinbouw
Vergrijzing is het grote probleem in de Japanse land- en tuinbouw. Daarom richt het onderzoek in dit land zich op arbeidsbesparing, lichtere arbeid en verbetering van de produktkwaliteit. De introductie van hoogwaardige techniek op de bedrijven moet de sector bovendien weer aantrekkelijk maken voor de nieuwe generatie
Tuinbouwtechniek in Japan; verslag van een studiereis
Looijesteijn, F.X.C. - \ 1995
Unknown Publisher - 32 p.
Een test van enkele temperatuurrecorders
Nieuwenhuizen, G.H. van; Looijesteijn, F.X.C. - \ 1979
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2095) - 6
meting - methodologie - fysica - regulatie - temperatuur - thermodynamica - measurement - methodology - physics - regulation - temperature - thermodynamics
Nieuwe werkmethoden bij het invriezen van leliebollen
Boerrigter, H.A.M. ; Looijesteijn, F.X.C. - \ 1979
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2055) - 10
bollen - liliaceae - liliales - sierplanten - bulbs - ornamental plants
Invloed van de ventilatie, circulatie en mechanische beschadiging van de bollen op het optreden van heetstookschade bij hyacinten
Looijesteijn, F.X.C. ; Boerrigter, H.A.M. ; Veltman, B.J.L. - \ 1978
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2021) - 17
hyacinthus - bloembollen - opslag als stortgoed - opslagverliezen - voorraadbederf - ornamental bulbs - bulk storage - storage losses - storage decay
A second trial transport of flower bulbs to Guernsey
Looijesteijn, F.X.C. - \ 1977
Wageningen : Sprenger Instituut (Rapport / Sprenger instituut no. 1987) - 10
bollen - groot-brittannië - liliaceae - liliales - nederland - sierplanten - transport - bulbs - great britain - netherlands - ornamental plants
Verslag van een proeftransport met bloembollen naar Guernsey
Looijesteijn, F.X.C. - \ 1975
Wageningen : Sprenger Instituut (Rapport / Sprenger instituut no. 1986) - 11
bollen - groot-brittannië - liliaceae - liliales - nederland - sierplanten - transport - bulbs - great britain - netherlands - ornamental plants
Vergelijkend onderzoek van twee typen kunststoffust tegenover twee typen bestaand fust waar het betreft het resultaat van nadrogen en bewaren van tulpebollen en gladiolenknollen in dit fust
Rudolpgij, J.W. ; Looijesteijn, F.X.C. ; Veltman, B.J.L. - \ 1974
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 1898) - 36
balen - dozen - drogen - bloembollen - verpakken - tulipa - gladiolus - tulpen - opslag - bales - boxes - drying - ornamental bulbs - packing - tulips - storage
Het drogen van bloembollen in stapelkisten
Rudolphij, J.W. ; Looijesteijn, F.X.C. ; Laar, J. van - \ 1972
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 1812) - 31
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