Lectin receptor kinase 79, a putative target of the Phytophthora infestans effector IPI-O
Bouwmeester, K. ; Klamer, S. ; Gouget, A. ; Haget, N. ; Canut, H. ; Govers, F. - \ 2008
In: Biology of Plant-Microbe Interactions / Lorito, M., Woo, S.L., Scala, F., St. Paul, MN, USA : International Society for Molecular Plant-Microbe Interactions (Biology of plant-microbe interactions 6) - ISBN 9780965462556
|Effectors, their targets and host responses in the pathosystem Cladosporium fulvum-tomato
Wit, P.J.G.M. de; Stergiopoulos, I. ; Klooster, J.W. van t; Esse, H.P. van; Fradin, E.F. ; Ellendorff, U. ; Gabriëls, S.H.E.J. ; Stulemeijer, I.J.E. ; Bolton, M.D. ; Borrás-Hidalgo, O. ; Tameling, W.I.L. ; Abd-El-Haliem, A.M. ; Berg-Velthuis, G.C.M. van den; Vervoort, J.J.M. ; Boeren, S. ; Joosten, M.H.A.J. ; Thomma, B.P.H.J. - \ 2008
In: Biology of Plant-Microbe Interactions CD / Lorito, M., Woo, S.L., Scala, F., St. Paul, MN, USA : International Society for Molecular Plant-Microbe Interactions (Biology of Plant-Microbe Interactions 6) - p. np - np.
Studies on the effect of amadoriase from Aspergillus fumigatus on peptide and protein glycation in vitro
Capuano, Edoardo ; Fedele, Francalisa ; Mennella, Carmela ; Visciano, Marianna ; Napolitano, Aurora ; Lanzuise, Stefania ; Ruocco, Michelina ; Lorito, Matteo ; Castillo, María Dolores Del; Fogliano, Vincenzo - \ 2007
Journal of Agricultural and Food Chemistry 55 (2007)10. - ISSN 0021-8561 - p. 4189 - 4195.
Amadori products - Amadoriase I - Browning - Protein glycation
Amadoriase I is a fructosyl amine oxidase from Aspergillus fumigatus that catalyzes the oxidation of Amadori products (APs) producing glucosone, H 2O2, and the corresponding free amine. All the enzymes of this family discovered so far only deglycate small molecular weight products and are inactive toward large molecular weight substrates, such as glycated BSA or ribonuclease A. Therefore, they cannot be used to reverse protein glycation occurring in diabetes or in foods. In this paper, the effect of Amadoriase I added during the in vitro reaction between glucose and peptides having different polarities or proteins with molecular weights ranging from to 5 to 66 kDa was tested. The formation of APs was monitored by ESI-MS of intact glycated protein or peptides and by measuring the Nε-(1-deoxy-D-fructos-1-yl)-L- lysine and furosine concentrations. Results showed that the formation of APs is reduced up to 80% when peptides and glucose are incubated in the presence of Amadoriase. The effect is more evident for hydrophobic peptides. In protein-glucose systems, the effect was dependent on the molecular weight and steric hindrance being negligible for BSA and at a maximum for insulin, where the formation of APs was reduced up to 60%. These findings indicate new potential applications of Amadoriase I as an efficient tool for inhibiting protein glycation in real food systems.
|A novel application of Trichoderma spp. as a tool to deliver protective molecules into plants: transformation of T. atroviride P1 with the avirulence gene avr4 from Cladosporium fulvum
Ruocco, M. ; Lanzuise, S. ; Woo, S. ; Scala, F. ; Wit, P.J.G.M. de; Joosten, M.H.A.J. ; Lorito, M. - \ 2003
In: Volume of Abstracts: 11-th International Congress on Molecular Plant-Microbe Interactions, St.-Petersburg, Russia, 18-26 July 2003 St.-Petersburg, Russia : All-Russia Research Institute for Agricultural Microbiology - p. 368 - 368.
|Potential for exploitation of ATP-binding cassette transporters in biological control
Sorbo, G. Del; Ruocco, M. ; Lorito, M. ; Scala, F. ; Zoina, A. ; Andrade, A.C. ; Waard, M.A. de - \ 1998
In: Molecular approaches in biological control / Duffy, B., Rosenberger, U., Defago, G., - p. 241 - 246.
|Studies on the role of ABC transporters in multidrug resistance and pathogenicity of filamentous fungi.
Sorbo, G. del; Ruocco, M. ; Andrade, A. ; Schoonbeek, H. ; Lorito, M. ; Zoina, A. ; Waard, M.A. de; Scala, F. - \ 1998
In: 7th International Congress of Plant Pathology, Edinburgh, UK (1998) 5.5.7
|Potential for exploitation of AT-binding-cassette transporters in biological control.
Sorbo, G. del; Lorito, M. ; Scala, F. ; Zoin, A. ; Andrade, A.C. ; Waard, M.A. de - \ 1997
In: IOBC-Proceedings EFPP Workshop Molecular Approaches in Biological Control, Delemont, Switserland - p. 1 - 1.