- E.F. Crowell (1)
- D.C.T. Dees (1)
- T. Desprez (2)
- M. Gonneau (1)
- Y. Gu (1)
- T.M. Have van der (3)
- H. Hofte (1)
- H. Höfte (1)
- G.O. Keijl (3)
- Tori L. Speicher (1)
- F.A.M. Leermakers (1)
- L. Lei (1)
- C. Lessa Alvim Kamei (1)
- Xingxing Li (1)
- S. Li (1)
- Luisa M. Trindade (1)
- Nasim Mansoori (1)
- J. Mansoori (3)
- N. Mansoori Zangir (4)
- V.V. Morozov (3)
- S. Pagant (1)
- S. Robert (1)
- Ian S. Wallace (1)
- C. Somerville (1)
- E. Spruijt (1)
- M.A.C. Stuart (1)
- Ming Tien (1)
- J.F.P. Timmers (1)
- H. Timpano (1)
- L.M. Trindade (3)
- T. Vain (1)
- S. Vernhettes (3)
- J.P. Vincken (1)
- R.G.F. Visser (2)
- Alison W. Roberts (1)
Convergent evolution of hetero-oligomeric cellulose synthesis complexes in mosses and seed plants
Li, Xingxing ; Speicher, Tori L. ; Dees, Dianka C.T. ; Mansoori, Nasim ; McManus, John B. ; Tien, Ming ; Trindade, Luisa M. ; Wallace, Ian S. ; Roberts, Alison W. - \ 2019
The Plant Journal (2019). - ISSN 0960-7412
cell wall - cellulose - cellulose synthase - cellulose synthesis complex - convergent evolution - Physcomitrella patens
In seed plants, cellulose is synthesized by rosette-shaped cellulose synthesis complexes (CSCs) that are obligate hetero-oligomeric, comprising three non-interchangeable cellulose synthase (CESA) isoforms. The moss Physcomitrella patens has rosette CSCs and seven CESAs, but its common ancestor with seed plants had rosette CSCs and a single CESA gene. Therefore, if P. patens CSCs are hetero-oligomeric, then CSCs of this type evolved convergently in mosses and seed plants. Previous gene knockout and promoter swap experiments showed that PpCESAs from class A (PpCESA3 and PpCESA8) and class B (PpCESA6 and PpCESA7) have non-redundant functions in secondary cell wall cellulose deposition in leaf midribs, whereas the two members of each class are redundant. Based on these observations, we proposed the hypothesis that the secondary class A and class B PpCESAs associate to form hetero-oligomeric CSCs. Here we show that transcription of secondary class A PpCESAs is reduced when secondary class B PpCESAs are knocked out and vice versa, as expected for genes encoding isoforms that occupy distinct positions within the same CSC. The class A and class B isoforms co-accumulate in developing gametophores and co-immunoprecipitate, suggesting that they interact to form a complex in planta. Finally, secondary PpCESAs interact with each other, whereas three of four fail to self-interact when expressed in two different heterologous systems. These results are consistent with the hypothesis that obligate hetero-oligomeric CSCs evolved independently in mosses and seed plants and we propose the constructive neutral evolution hypothesis as a plausible explanation for convergent evolution of hetero-oligomeric CSCs.
The Cellulase KORRIGAN Is Part of the Cellulose Synthase Complex
Vain, T. ; Crowell, E.F. ; Timpano, H. ; Biot, E. ; Desprez, T. ; Mansoori Zangir, N. ; Trindade, L.M. ; Pagant, S. ; Robert, S. ; Hofte, H. ; Gonneau, M. ; Vernhettes, S. - \ 2014
Plant Physiology 165 (2014)4. - ISSN 0032-0889 - p. 1521 - 1532.
secondary cell-wall - arabidopsis-thaliana - genetic-evidence - plasma-membrane - higher-plants - endo-1,4-beta-glucanase - microtubules - trafficking - elongation - endo-1,4-beta-d-glucanase
Plant growth and organ formation depend on the oriented deposition of load-bearing cellulose microfibrils in the cell wall. Cellulose is synthesized by a large relative molecular weight cellulose synthase complex (CSC), which comprises at least three distinct cellulose synthases. Cellulose synthesis in plants or bacteria also requires the activity of an endo-1,4-beta-D-glucanase, the exact function of which in the synthesis process is not known. Here, we show, to our knowledge for the first time, that a leaky mutation in the Arabidopsis (Arabidopsis thaliana) membrane-bound endo-1,4-beta-D-glucanase KORRIGAN1 (KOR1) not only caused reduced CSC movement in the plasma membrane but also a reduced cellulose synthesis inhibitor-induced accumulation of CSCs in intracellular compartments. This suggests a role for KOR1 both in the synthesis of cellulose microfibrils and in the intracellular trafficking of CSCs. Next, we used a multidisciplinary approach, including live cell imaging, gel filtration chromatography analysis, split ubiquitin assays in yeast (Saccharomyces cerevisiae NMY51), and bimolecular fluorescence complementation, to show that, in contrast to previous observations, KOR1 is an integral part of the primary cell wall CSC in the plasma membrane.
KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery
Mansoori Zangir, N. ; Timmers, J.F.P. ; Desprez, T. ; Lessa Alvim Kamei, C. ; Dees, D.C.T. ; Vincken, J.P. ; Visser, R.G.F. ; Höfte, H. ; Vernhettes, S. ; Trindade, L.M. - \ 2014
PLoS ONE 9 (2014)11. - ISSN 1932-6203
secondary cell-wall - arabidopsis-thaliana - endo-1,4-beta-glucanase - expression - membranes - protein - system - plants - gene - endo-1,4-beta-d-glucanase
Cellulose is synthesized by the so called rosette protein complex and the catalytic subunits of this complex are the cellulose synthases (CESAs). It is thought that the rosette complexes in the primary and secondary cell walls each contains at least three different non-redundant cellulose synthases. In addition to the CESA proteins, cellulose biosynthesis almost certainly requires the action of other proteins, although few have been identified and little is known about the biochemical role of those that have been identified. One of these proteins is KORRIGAN (KOR1). Mutant analysis of this protein in Arabidopsis thaliana showed altered cellulose content in both the primary and secondary cell wall. KOR1 is thought to be required for cellulose synthesis acting as a cellulase at the plasma membrane–cell wall interface. KOR1 has recently been shown to interact with the primary cellulose synthase rosette complex however direct interaction with that of the secondary cell wall has never been demonstrated. Using various methods, both in vitro and in planta, it was shown that KOR1 interacts specifically with only two of the secondary CESA proteins. The KOR1 protein domain(s) involved in the interaction with the CESA proteins were also identified by analyzing the interaction of truncated forms of KOR1 with CESA proteins. The KOR1 transmembrane domain has shown to be required for the interaction between KOR1 and the different CESAs, as well as for higher oligomer formation of KOR1.
Sedimentation dynamics and equilibrium profiles in multicomponent mixtures of colloidal particles
Spruijt, E. ; Biesheuvel, P.M. - \ 2014
Journal of Physics-Condensed Matter 26 (2014)7. - ISSN 0953-8984
density gradient centrifugation - macroscopic electric-field - screened charged colloids - equation-of-state - hard-spheres - analytical ultracentrifugation - diffusion equilibrium - relative motion - binary-mixtures - fluidized-beds
In this paper we give a general theoretical framework that describes the sedimentation of multicomponent mixtures of particles with sizes ranging from molecules to macroscopic bodies. Both equilibrium sedimentation profiles and the dynamic process of settling, or its converse, creaming, are modeled. Equilibrium profiles are found to be in perfect agreement with experiments. Our model reconciles two apparently contradicting points of view about buoyancy, thereby resolving a long-lived paradox about the correct choice of the buoyant density. On the one hand, the buoyancy force follows necessarily from the suspension density, as it relates to the hydrostatic pressure gradient. On the other hand, sedimentation profiles of colloidal suspensions can be calculated directly using the fluid density as apparent buoyant density in colloidal systems in sedimentation–diffusion equilibrium (SDE) as a result of balancing gravitational and thermodynamic forces. Surprisingly, this balance also holds in multicomponent mixtures. This analysis resolves the ongoing debate of the correct choice of buoyant density (fluid or suspension): both approaches can be used in their own domain. We present calculations of equilibrium sedimentation profiles and dynamic sedimentation that show the consequences of these insights. In bidisperse mixtures of colloids, particles with a lower mass density than the homogeneous suspension will first cream and then settle, whereas particles with a suspension-matched mass density form transient, bimodal particle distributions during sedimentation, which disappear when equilibrium is reached. In all these cases, the centers of the distributions of the particles with the lowest mass density of the two, regardless of their actual mass, will be located in equilibrium above the so-called isopycnic point, a natural consequence of their hard-sphere interactions. We include these interactions using the Boublik–Mansoori–Carnahan–Starling–Leland (BMCSL) equation of state. Finally, we demonstrate that our model is not limited to hard spheres, by extending it to charged spherical particles, and to dumbbells, trimers and short chains of connected beads.
Complexes with mixed primary and secondary cellulose synthases are functional in Arabidopsis plants
Carroll, A. ; Mansoori Zangir, N. ; Li, S. ; Lei, L. ; Vernhettes, S. ; Visser, R.G.F. ; Somerville, C. ; Gu, Y. ; Trindade, L.M. - \ 2012
Plant Physiology 160 (2012)2. - ISSN 0032-0889 - p. 726 - 737.
cell-wall synthesis - bimolecular fluorescence complementation - proteins in-vivo - cesa gene family - plasma-membrane - split-ubiquitin - populus-trichocarpa - biosynthesis - expression - visualization
In higher plants, cellulose is synthesized by so-called rosette protein complexes with cellulose synthases (CESAs) as catalytic subunits of the complex. The CESAs are divided into two distinct families, three of which are thought to be specialized for the primary cell wall and three for the secondary cell wall. In this article, the potential of primary and secondary CESAs forming a functional rosette complex has been investigated. The membrane-based yeast two-hybrid and biomolecular fluorescence systems were used to assess the interactions between three primary (CESA1, CESA3, CESA6), and three secondary (CESA4, CESA7, CESA8) Arabidopsis (Arabidopsis thaliana) CESAs. The results showed that all primary CESAs can physically interact both in vitro and in planta with all secondary CESAs. Although CESAs are broadly capable of interacting in pairwise combinations, they are not all able to form functional complexes in planta. Analysis of transgenic lines showed that CESA7 can partially rescue defects in the primary cell wall biosynthesis in a weak cesa3 mutant. Green fluorescent protein-CESA protein fusions revealed that when CESA3 was replaced by CESA7 in the primary rosette, the velocity of the mixed complexes was slightly faster than the native primary complexes. CESA1 in turn can partly rescue defects in secondary cell wall biosynthesis in a cesa8ko mutant, resulting in an increase of cellulose content relative to cesa8ko. These results demonstrate that sufficient parallels exist between the primary and secondary complexes for cross-functionality and open the possibility that mixed complexes of primary and secondary CESAs may occur at particular times.
Characterising the cellulose synthase complexes of cell walls
Mansoori Zangir, N. - \ 2012
Wageningen University. Promotor(en): Richard Visser, co-promotor(en): Luisa Trindade. - S.l. : s.n. - ISBN 9789461732958 - 162
planten - celwanden - cellulose - biosynthese - enzymen - enzymactiviteit - eiwitten - katalyse - genetische kartering - genomica - plants - cell walls - cellulose - biosynthesis - enzymes - enzyme activity - proteins - catalysis - genetic mapping - genomics
One of the characteristics of the plant kingdom is the presence of a structural cell wall. Cellulose is a major component in both the primary and secondary cell walls of plants. In higher plants cellulose is synthesized by so called rosette protein complexes with cellulose synthases (CESAs) as the catalytic subunits of the complex. The objective of the research presented in this thesis was to generate more in-depth knowledge in cellulose biosynthesis and to this aim better characterize and understand the cellulose synthase complex and its components by notably investigating the similarities and differences between the CESAs in the primary and secondary cellulose complex and identifying the various interacting proteins forming the complex in the plant cell wall. KORRIGAN and specific isoforms of sucrose synthase were shown to be co-localized and physically interact with the CESAs in the Cellulose Synthase Complex at the plasma membrane supporting their participation in cellulose biosynthesis in Arabidopsis.
Self-consistent field theory of protein adsorption in a non-Gaussian polyelectrolyte brush
Biesheuvel, P.M. ; Leermakers, F.A.M. ; Stuart, M.A.C. - \ 2006
Physical Review. E, Statistical nonlinear, and soft matter physics 73 (2006)1. - ISSN 1539-3755 - 9 p.
including charge regulation - electrical double-layer - grafted polymer brush - finite extensibility - free-energy - model - sedimentation - equilibrium - interfaces - equation
To describe adsorption of globular protein molecules in a polyelectrolyte brush we use the strong-stretching approximation of the Edwards self-consistent field equation, combined with corrections for a non-Gaussian brush. To describe chemical potentials in this mixture of (globular) species of widely varying sizes (ions, brush polyelectrolyte segments, globular protein molecules), we use the Boublik-Mansoori-Carnahan-Starling-Leland equation of state derived for polydisperse mixtures of spherical particles. The polyelectrolyte chain is described in this approach as a string of beads with the beads of a size related to the chain diameter. We use the one-dimensional Poisson equation to describe the electrostatic field and include the ionizable character of both the brush polyions and the protein molecules. This model explains the experimental observation of high amounts of protein adsorption in a polyacid brush for pH values above the isoelectric point of the protein as being due to charge reversal of the protein molecules upon entry in the brush. We find a distinct minimum in protein concentration near the edge of the brush. With increasing pH this barrier to protein transfer becomes larger, but much less so when we increase the ionic strength, a difference that might relate to an experimentally observed difference in the protein release rate in these two cases. A free energy analysis shows that the release of small ions from the brush and the increase of brush ionization are the two driving forces for protein adsorption in a like-charged brush
|Waterbirds in coastal wetlands along the Persian Gulf coast of Iran, January-February 2000
Have, T.M. van der; Keijl, G.O. ; Mansoori, J. ; Morozov, V.V. - \ 2002
Zoology in the Middle East 26 (2002). - ISSN 0939-7140 - p. 71 - 88.
Searching for Slender-billed Curlews in Iran, January-February 2000
Have, T.M. van der; Keijl, G.O. ; Mansoori, J. ; Morozov, V.V. - \ 2001
Zeist : WIWO (WIWO-report 72 )
numenius - vogels - watervogels - wetlands - inventarisaties - monitoring - iran - waadvogels - numenius - birds - waterfowl - wetlands - inventories - monitoring - iran - waders
Some interesting bird observations from the coast of Iran, January-February 2000
Keijl, G.O. ; Have, T.M. van der; Mansoori, J. ; Morozov, V.V. - \ 2000
Sandgrouse 23 (2000)1. - ISSN 0260-4736 - p. 44 - 48.
Observations of 15 species, made during a survey of wetlands on Iran's Arabian Gulf coast, in January-February 2000, are presented. These included two potential first records in Iran, Great Knot Calidris tenuirostris and Brown-throated Sand Martin Riparia paludicola, an apparently important wintering area for Dead Sea Sparrow Passer moabiticus, records of several vagrants and scarce winter visitors, and a potentially significant range extension for the near endemic Iraq Babbler Turdoides altirostris. In addition, the area surveyed appears to be of considerable significance to wintering waterbirds, particularly waders. More than 53,000 waterbirds of 82 species were counted during the survey, including globally threatened species such as Dalmatian Pelican Pelecanus crispus, Marbled Teal Marmaronetta angustirostris, Ferruginous Duck Aythya nyroca, and White-tailed Haliaeetus albicilla, Greater Spotted Aquila clanga and Imperial Eagles A. heliaca