Tomato Chlorotic Spot Virus (TCSV) Putatively Incorporated a Genomic Segment of Groundnut Ringspot Virus (GRSV) Upon a Reassortment Event
Silva, João Marcos Fagundes ; Oliveira, Athos Silva de; Almeida, Mariana Martins Severo de; Kormelink, Richard ; Nagata, Tatsuya ; Resende, Renato Oliveira - \ 2019
Viruses 11 (2019)2. - ISSN 1999-4915
groundnut ringspot virus - reassortment - tomato chlorotic spot virus - tospovirus - virus evolution
Tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV) share several genetic and biological traits. Both of them belong to the genus Tospovirus (family Peribunyaviridae), which is composed by viruses with tripartite RNA genome that infect plants and are transmitted by thrips (order Thysanoptera). Previous studies have suggested several reassortment events between these two viruses, and some speculated that they may share one of their genomic segments. To better understand the intimate evolutionary history of these two viruses, we sequenced the genomes of the first TCSV and GRSV isolates ever reported. Our analyses show that TCSV and GRSV isolates indeed share one of their genomic segments, suggesting that one of those viruses may have emerged upon a reassortment event. Based on a series of phylogenetic and nucleotide diversity analyses, we conclude that the parental genotype of the M segment of TCSV was either eliminated due to a reassortment with GRSV or it still remains to be identified.
The Tomato spotted wilt virus cell-to-cell movement protein (NSM) triggers a hypersensitive response in Sw-5 containing resistant tomato lines and Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy.
Hallwass, M. ; Silva de Oliveira, A. ; Dianese, E.C. ; Lohuis, D. ; Boiteux, L.S. ; Inoue-Nagata, A.K. ; Resende, R.O. de; Kormelink, R.J.M. - \ 2014
Molecular Plant Pathology 15 (2014)9. - ISSN 1464-6722 - p. 871 - 880.
mosaic-virus - lycopersicon-esculentum - nonstructural protein - capsicum-chinense - coat protein - plant-cells - rna segment - tswv - tospovirus - tobacco
Although the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum SR1 plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N.benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive response (HR). Using these plants, from all structural and non-structural TSWV proteins tested, the TSWV cell-to-cell movement protein (NSM) was confirmed as the Avr determinant using a Potato virus X (PVX) replicon or a non-replicative pEAQ-HT expression vector system. HR was induced in Sw-5b-transgenic N.benthamiana as well as in resistant near-isogenic tomato lines after agroinfiltration with a functional cell-to-cell movement protein (NSM) from a resistance-inducing (RI) TSWV strain (BR-01), but not with NSM from a Sw-5 resistance-breaking (RB) strain (GRAU). This is the first biological demonstration that Sw-5-mediated resistance is triggered by the TSWV NSM cell-to-cell movement protein.
Development of a locus-specific, co-dominant SCAR marker for assisted-selection of the Sw-5 (Tospovirus resistance) gene cluster in a wide range of tomato accessions
Dianese, E.C. ; Fonseca, M.E.N. ; Goldbach, R.W. ; Kormelink, R.J.M. ; Inoue-Nagata, A.K. ; Resende, R.O. de; Boiteux, L.S. - \ 2010
Molecular Breeding 25 (2010)1. - ISSN 1380-3743 - p. 133 - 142.
spotted-wilt-virus - lycopersicon-esculentum - thrips transmission - tswv resistance - rapd markers - peruvianum - sw5
The best levels of broad-spectrum Tospovirus resistance reported in tomatoes thus far are conferred by the Sw-5 locus. This locus contains at least five paralogues (denoted Sw-5a through Sw-5e), of which Sw-5b represents the actual resistance gene. Here we evaluated a panel of seven PCR primer pairs matching different sequences within a genomic region spanning the Sw-5a and Sw-5b gene cluster. Primer efficiency evaluation was done employing tomato isolines with and without the Sw-5 locus. One primer pair produced a single and co-dominant polymorphism between susceptible and resistant isolines. Sequence analysis of these amplicons indicated that they were specific for the Sw-5 locus and their differences were due to insertions/deletions. The polymorphic SCAR amplicon encompass a conserved sequence of the promoter region of the functional Sw-5b gene, being located in the position -31 from its open reading frame. This primer pair was also evaluated in field assays and with a collection of accessions known to be either susceptible or resistant to tospoviruses. An almost complete correlation was found between resistance under greenhouse/field conditions and the presence of the marker. Therefore, this primer pair is a very useful tool in marker-assisted selection systems in a large range of tomato accessions.
Molecular and biological characterization of Tomato chlorotic mottle virus suggests that recombination underlies the evolution and diversity of Brazilian tomato begomoviruses
Ribeiro, S.G. ; Martin, D.P. ; Lacorte, C. ; Simoes, I.C. ; Orlandini, D.R.S. ; Inoue-Nagata, A.K. - \ 2007
Phytopathology 97 (2007)6. - ISSN 0031-949X - p. 702 - 711.
whitefly-transmitted geminiviruses - leaf-curl-virus - abutilon-mosaic-virus - dna-b - nicotiana-benthamiana - replication - emergence - sequences - disease - origin
Tomato chlorotic mottle virus (ToCMoV) is an emerging begomovirus species widely distributed throughout tomato-growing regions of Brazil. ToCMoV appears to have expanded its geographic range recently, invading tomato-growing areas that were free of begomovirus infection before 2004. We have determined the first complete genome sequence of an infectious ToCMoV genome (isolate BA-Se1), which is the first begomovirus species isolated in the northeast of Brazil. When introduced by particle bombardment into tomato, the cloned ToCMoV-[BA-Se1] DNA-A and DNA-B components caused typical chlorotic mottle symptoms. The cloned virus was whitefly-transmissible and, although it was infectious in hosts such as Nicotiana benthamiana, pepper, tobacco, and Nicandra physaloides, it was unable to infect Arabidopsis thaliana, bean, N. glutinosa, and Datura metel. Sequence and biological analyses indicate that ToCMoV-[BA-Se1] is a typical New World begomovirus sp. requiring both DNA-A and DNA-B components to establish systemic infections. Although evidence of multiple recombination events was detected within the ToCMoV-[BA-Se1] DNA-A, they apparently occurred relatively long ago, implying that recombination probably has not contributed to the recent emergence of this species.
|Occurrence of begomovirus in tomato and other plant species in Central Brazil
Inoue-Nagata, A.K. ; Giordano, L.B. ; Fonseca, M.E.N. ; Ribeiro, S.G. ; Avila, L.C. ; Albuquerque, L.C. ; Boiteux, L.S. - \ 2005
|Potato deforming mosaic virus is possibly a variant of Tomato yellow vein streak virus
Ribeiro, S.G. ; Inoue-Nagata, A.K. ; Daniels, J. ; Avila, A.C. de - \ 2004
|Quantitative trait loci for clubroot resistance in Brassica oleracea
Voorrips, R.E. ; Jongerius, M.C. ; Kanne, H.J. - \ 2003
In: Brassica and legumes: from genome structure to breeding / Nagata, T., Tabata, S., Springer Verlag (Biotechnology in agriculture and forestry 52) - ISBN 9783540427285 - p. 87 - 104.
Pepper yellow mosaic virus, a new potyvirus in sweet-pepper. Archives of Virology
Inoue-Nagata, A.K. ; Fonseca, M.E.N. ; Resende, R.O. de; Boiteux, L.S. ; Monte, D.C. ; Dusi, A.N. ; Ávila, A.C. de; Vlugt, R.A.A. van der - \ 2002
Archives of Virology 147 (2002)4. - ISSN 0304-8608 - p. 849 - 855.
A potyvirus was found causing yellow mosaic and veinal banding in sweetpepper in Central and Southeast Brazil. The sequence analysis of the 3' terminal region of the viral RNA revealed a coat protein of 278 amino acids, followed by 275 nucleotides in the 3'-untranslated region preceding a polyadenylated tail. The virus shared 77.4% coat protein amino acid identity with Pepper severe mosaic virus, the closest Potyvirus species. The 3'-untranslated region was highly divergent from other potyviruses. Based on these results, the virus found in sweetpepper plants could be considered as a new potyvirus. The name Pepper yellow mosaic virus (PepYMV) is suggested
Expression of the movement protein of tomato spotted wilt virus in the insect vector Frankliniella occidentalis.
Storms, M. ; Nagata, T. ; Kormelink, R. ; Lent, J.W.M. van; Goldbach, R.W. - \ 2002
Archives of Virology 147 (2002). - ISSN 0304-8608 - p. 825 - 831.
Tomato spotted wilt virus (TSWV) is able to infect both its botanical hosts and its insect vector (thrips). In plant tissue the NSM protein of TSWV functions as viral movement protein (MP), aggregating into plasmodesma-penetrating tubules to establish cell-to-cell movement. As upon heterologous expression NSM was able to form similar tubules on the surface of insect (Spodoptera frugiperda) cells, we have now investigated the expression and cellular manifestation of this protein in infected thrips tissue. It is shown that NSM, though detectably expressed in both the L2 larval and adult thrips stages, does not aggregate into tubules, indicating that this requirement is associated to its function as MP in plants, and raising the question if NSM has a function at all during the insect life cycle of TSWV.
Factors determining vector competence and specificity for transmission of Tomato spotted wilt virus
Nagata, T. ; Inoue-Nagata, A.K. ; Lent, J. van; Goldbach, R. ; Peters, D. - \ 2002
Journal of General Virology 83 (2002). - ISSN 0022-1317 - p. 663 - 671.
|Tomato chlorotic mottle virus: a novel tomato begomovirus from Brazil
Ribeiro, S.G. ; Lacorte, C. ; Inoue-Nagate, A.K. ; Carmo, I. de; Orlandini, D. ; Andrade, E.C. de; Nagata, T. ; Zerbini, F.M. - \ 2001
In: Comparative Virology : Proceedings of ssDNA Viruses of Plants, Birds, Pigs and Primates, Saint-Malo, France, 2001 Saint-Malo : S.1. - p. 47 - 47.
|An anatomical perspective of tospovirus transmission
Nagata, T. ; Peters, D. - \ 2001
In: Virus-Insect-Plant Interactions / Harris, K.F., Smith, O.P., Duffus, J.E., San Diego : Academic Press - ISBN 9780123276810 - p. 51 - 67.
Impeded thrips transmission of defective tomato spotted wilt virus isolates
Nagata, T. ; Inoue-Nagata, A.K. ; Prins, M. ; Goldbach, R. ; Peters, D. - \ 2000
Phytopathology 90 (2000)5. - ISSN 0031-949X - p. 454 - 459.
Two defective RNA-containing isolates (Pe-1 and 16-2) and an envelope-deficient (env(^–)) isolate of Tomato spotted wilt virus (TSWV) were tested for their transmissibility by Frankliniella occidentalis. The Pe-1 isolate contained a truncated L RNA segment that barely interfered with symptom expression and replication of the wild-type (wt) L RNA segment. This isolate was transmitted with an efficiency of 51°a value comparable to that found for wt TSWV (54Ž Isolate 16-2, which contained a genuine defective interfering L RNA as concluded from its ability to suppress wt L RNA synthesis and attenuation of symptom expression, was not transmitted at all. The midguts of all larvae that ingested Pe-1 became infected, whereas limited midgut infections were found in 24␘f the larvae that ingested 16-2. This difference in infection could be explained by the presence of a low number of infectious units in the inoculum ingested from plants as demonstrated in infection experiments and verified by northern blot analysis. The env(^–) isolate failed to infect the midgut after ingestion and could not be transmitted by any thrips stage. This isolate also cannot infect primary thrips cell cultures. Taken together, these results suggest that the envelope of TSWV contains the determinants required for binding and subsequent infection of thrips cells.
|Infection of transmitting and non-transmitting thrips by tomato spotted wilt virus
Nagata, T. ; Goldbach, R.W. ; Peters, D. - \ 1999
In: VIIth International Plant Virus Epidemiology Symposium : Plant Virus Epidemiology : Current status and future prospects : 11 - 16 April 1999, Aguadulce (Almeria), Spain : book of abstracts. - [S.l.] : [s.n.], 1999 - p. 38 - 38.
|Infection and transmission of tomato spotted wilt virus mutants by thrips, Frankliniella occidentalis
Nagata, T. ; Inoue-Nagata, A.K. ; Goldbach, R. ; Peters, D. - \ 1999
Fitopatologia Brasileira 24 (1999). - ISSN 0100-4158 - p. 260 - 260.
Tissue tropism related to vector competence of Frankliniella occidentalis for tomato spotted wilt tospovirus
Nagata, T. ; Inoue-Nagata, A.K. ; Smid, H.M. ; Goldbach, R. ; Peters, D. - \ 1999
Journal of General Virology 80 (1999). - ISSN 0022-1317 - p. 507 - 515.
Competence and specificity of thrips in the transmission of tomato spotted wilt virus
Nagata, T. - \ 1999
Agricultural University. Promotor(en): R.W. Goldbach; D. Peters. - S.l. : Nagata - ISBN 9789058080769 - 96
tomatenbronsvlekkenvirus - plantenvirussen - plantenziekteverwekkers - plantenziekten - thrips - vectoren - transmissie - landbouwkundige entomologie - tomato spotted wilt virus - plant viruses - plant pathogens - plant diseases - thrips - vectors - transmission - agricultural entomology
The study described in this thesis aims to elucidate the fate and pathway of ingested TSWV in thrips during their development from larvae to adult. Insight in this process will contribute to a better understanding of the factors regulating and determining vector competence and specificities.
Analysis of the differences in virus susceptibility among thrips species or populations was approached by infection of cell cultures. The methodology developed and the media used to prepare primary cell cultures of the species F. occidentalis and T. tabaci are described and discussed in Chapter 2. The cultures obtained were derived from an efficiently transmitting F. occidentalis population and from a non-transmitting T.tabaci population which was not able to transmit the virus. The results obtained by inoculation of these cultures with preparations of purified TSWV particles are described in Chapter 3. To analyse the tissue tropism of TSWV in thrips in relation to its vector competence, a novel histological technique, called whole mount immunofluorescent staining (WMIS) was developed (Chapter 4). Using this technique and other immunohistochemical techniques, infection of the midguts and salivary glands during the development of F. occidentalis thrips was described (Chapter 4). By the combination of all techniques, the temporal development of the virus infection in larvae and adults could be elucidated. To define the various barriers which may regulate the development of virus infection, specific TSWV mutants were used which failed either to infect the thrips or to convert the thrips in a transmitter after infection. Definite barriers were observed at the level of virus entry in the midgut epithelium or virus escape from the midgut to the salivary glands (Chapter 5). The pathway of the virus within the thrips and the mechanism determining the vector specificities were further unravelled by analysing the infection in thrips of a transmitting F. occidentalis population and a nontransmitting T. tabaci population (Chapter 6). Concluding remarks of this study is presented in Chapter 7.
Transmission of tomato spotted wilt tospovirus by Thrips tabaci populations originating from leek
Chatzivassiliou, E.K. ; Nagata, T. ; Katis, N.I. ; Peters, D. - \ 1999
Plant Pathology 48 (1999). - ISSN 0032-0862 - p. 700 - 706.
The transmission of tomato spotted wilt tospovirus (TSWV) by Thrips tabaci collected from leek was studied using the petunia local-lesion leaf-disc assay. After an acquisition-access period of 72 h given to newborn larvae up to 8 h old, the efficiency of transmission by adults was determined in three inoculation-access periods of 48 h. This efficiency varied for six T. tabaci populations from 0.7 to 11.6␒n experiments using the Greek TSWV isolate GR-04. Males were more efficient transmitters than females (19 out of 176 versus five out of 494). Frankliniella occidentalis transmitted the same virus with a higher efficiency (34.8Ž The transmission rate differed also among TSWV isolates, as shown in tests with four T. tabaci using two isolates. The virus was more efficiently acquired from infected leaf material of Datura stramonium than from that of Emilia sonchifolia. Plants of the latter species were more susceptible than Nicotiana tabacum in thrips transmission tests.
Increase of tospoviral diversity in Brazil with the identification of two new tospovirus species, one from Chrysanthemum and one from Zucchini
Bezerra, I.C. ; Resende, R.O. de; Pozzer, L. ; Nagata, T. ; Kormelink, R. ; Avila, A.C. de - \ 1999
Phytopathology 89 (1999)9. - ISSN 0031-949X - p. 823 - 830.
|Tomato spotted wilt virus defective interfering RNAs: important features of their generation and survival.
Inoue-Nagate, A. ; Kormelink, R. ; Sgro, J.Y. ; Nagata, T. ; Kitajima, E.W. ; Goldbach, R. ; Peters, D. - \ 1998
In: 4th International Symposium on Tospoviruses and Thrips in Floral and Vegetable Crops, Wageningen, The Netherlands - p. 4 - 5.