Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Antifouling Polymer Brushes via Oxygen-Tolerant Surface-Initiated PET-RAFT
    Kuzmyn, Andriy R. ; Nguyen, Ai T. ; Teunissen, Lucas W. ; Zuilhof, Han ; Baggerman, Jacob - \ 2020
    Langmuir 36 (2020)16. - ISSN 0743-7463 - p. 4439 - 4446.

    This work presents a new method for the synthesis of antifouling polymer brushes using surface-initiated photoinduced electron transfer-reversible addition-fragmentation chain-transfer polymerization with eosin Y and triethanolamine as catalysts. This method proceeds in an aqueous environment under atmospheric conditions without any prior degassing and without the use of heavy metal catalysts. The versatility of the method is shown by using three chemically different monomers: oligo(ethylene glycol) methacrylate, N-(2-hydroxypropyl)methacrylamide, and carboxybetaine methacrylamide. In addition, the light-triggered nature of the polymerization allows the creation of complex three-dimensional structures. The composition and topological structuring of the brushes are confirmed by X-ray photoelectron spectroscopy and atomic force microscopy. The kinetics of the polymerizations are followed by measuring the layer thickness with ellipsometry. The polymer brushes demonstrate excellent antifouling properties when exposed to single-protein solutions and complex biological matrices such as diluted bovine serum. This method thus presents a new simple approach for the manufacturing of antifouling coatings for biomedical and biotechnological applications.

    A global database of soil nematode abundance and functional group composition
    Hoogen, Johan van den; Geisen, Stefan ; Wall, Diana H. ; Wardle, David A. ; Traunspurger, Walter ; Goede, Ron G.M. de; Adams, Byron J. ; Ahmad, Wasim ; Ferris, Howard ; Bardgett, Richard D. ; Bonkowski, Michael ; Campos-Herrera, Raquel ; Cares, Juvenil E. ; Caruso, Tancredi ; Brito Caixeta, Larissa de; Chen, Xiaoyun ; Costa, Sofia R. ; Creamer, Rachel ; Cunha e Castro, José Mauro da; Dam, Marie ; Djigal, Djibril ; Escuer, Miguel ; Griffiths, Bryan S. ; Gutiérrez, Carmen ; Hohberg, Karin ; Kalinkina, Daria ; Kardol, Paul ; Kergunteuil, Alan ; Korthals, Gerard ; Krashevska, Valentyna ; Kudrin, Alexey A. ; Li, Qi ; Liang, Wenju ; Magilton, Matthew ; Marais, Mariette ; Martín, José Antonio Rodríguez ; Matveeva, Elizaveta ; Mayad, El Hassan ; Mzough, E. ; Mulder, Christian ; Mullin, Peter ; Neilson, Roy ; Nguyen, Duong T.A. ; Nielsen, Uffe N. ; Okada, Hiroaki ; Rius, Juan Emilio Palomares ; Pan, Kaiwen ; Peneva, Vlada ; Pellissier, Loïc ; Silva, Julio Carlos Pereira da; Pitteloud, Camille ; Powers, Thomas O. ; Powers, Kirsten ; Quist, Casper W. ; Rasmann, Sergio ; Moreno, Sara Sánchez ; Scheu, Stefan ; Setälä, Heikki ; Sushchuk, Anna ; Tiunov, Alexei V. ; Trap, Jean ; Vestergård, Mette ; Villenave, Cecile ; Waeyenberge, Lieven ; Wilschut, Rutger A. ; Wright, Daniel G. ; Keith, Aidan M. ; Yang, Jiuein ; Schmidt, Olaf ; Bouharroud, R. ; Ferji, Z. ; Putten, Wim H. van der; Routh, Devin ; Crowther, Thomas W. - \ 2020
    Scientific Data 7 (2020)1. - ISSN 2052-4463

    As the most abundant animals on earth, nematodes are a dominant component of the soil community. They play critical roles in regulating biogeochemical cycles and vegetation dynamics within and across landscapes and are an indicator of soil biological activity. Here, we present a comprehensive global dataset of soil nematode abundance and functional group composition. This dataset includes 6,825 georeferenced soil samples from all continents and biomes. For geospatial mapping purposes these samples are aggregated into 1,933 unique 1-km pixels, each of which is linked to 73 global environmental covariate data layers. Altogether, this dataset can help to gain insight into the spatial distribution patterns of soil nematode abundance and community composition, and the environmental drivers shaping these patterns.

    The 2019 Potato Wart Disease workshop: shared needs and future research directions
    Vossenberg, B.T.L.H. van de; Lee, T.A.J. van der; Nguyen, H.D.T. - \ 2020
    EPPO Bulletin 50 (2020)1. - ISSN 0250-8052 - p. 170 - 176.

    Potato wart disease is caused by the fungus Synchytrium endobioticum. A workshop on this disease was held in June 2019 at the Dutch National Plant Protection Organization (NPPO-NL) in Wageningen, the Netherlands. Over 60 participants from 20 countries and 36 organizations came together to share their knowledge via presentations and posters, to strengthen professional networks, and to identify shared needs and future directions for S. endobioticum research. The three major topics of the workshop were disease occurrence and management, understanding the pathogen, and host breeding and resistance. Many aspects of this disease were discussed and summarized in a shared statement. Future directions identified were (1) research initiatives are currently being initiated to develop standardized molecular viability tests and a molecular pathotyping test, (2) coordination is needed to come up with better standardization of the pathotyping bioassays and the set of differential cultivars described in EPPO PM 7/28 needs to be better available for testing, and (3) stacking of resistance genes is strongly recommended but resistance breeding alone is not enough and should be combined with other management strategies to contain potato wart disease. Furthermore, the attendees remarked that organizing a workshop every 2 or 3 years would be of great benefit to the potato wart disease research community.

    Changing opinion, knowledge, skill and behaviour of Vietnamese shrimp farmers by using serious board games
    Bosma, Roel H. ; Ha, Tran T.P. ; Hiep, Tong Quoc ; Phuong, Nguyen T.H. ; Ligtenberg, Arend ; Rodela, Romina ; Bregt, Arnold K. - \ 2020
    The Journal of Agricultural Education and Extension 26 (2020)2. - ISSN 1389-224X - p. 203 - 221.
    Purpose: Mekong Delta's shrimp farming contributes to socio-economic development but tends to reduce the mangrove area. On the one hand, NGOs advocate balancing ecology and economy, while on the other hand, the Vietnamese government supports intensifying shrimp production. The latter strategy increases shrimp diseases and marginalises smallholders. To influence the opinions, knowledge and behaviour of shrimp farmers, we developed and tested a serious board game. Approach: Through several iterations, we designed a realistic board game mimicking local shrimp farming. Then, after conducting three sessions of gameplay with farmers in three sites, we assessed how this gameplay influenced players. We used a semi-experimental set-up by collecting data on the day of the gameplay, and on two further time-points. Findings: After the gameplays, farmers reported to have learned about the risks of monoculture-intensive-shrimp system and the advantages of hybrid systems. Likert ratings showed that players adopted innovations faster and, in case of shrimp diseases, consulted commercial service-providers less, but with other farmers more. They confirmed to have learned that the best strategy for them would be to adopt hybrid systems, i.e. intensify part of the area of mixed-mangrove or improved-extensive shrimp farms. Theoretical implications: Realistic board games can create opportunities for social learning and training of groups which are not always easy to reach. Practical implications: Board games can support transfer of knowledge and skills to farmers, and can develop their critical thinking. Creation: The findings that board games are effective in the context of a top-down extension systems offer value opportunities.
    Data-driven process redesign: anticipatory shipping in agro-food supply chains
    Viet, Nguyen Quoc ; Behdani, Behzad ; Bloemhof, Jacqueline - \ 2020
    International Journal of Production Research 58 (2020)5. - ISSN 0020-7543
    agro-food - anticipatory shipping - association rule mining - multi-agent simulation - perishable - process redesign

    Anticipatory shipping uses historical order and customer data to predict future orders and accordingly ship products to the nearest distribution centres before customers actually place the orders. It is a method to meet the increasing customer requirements on delivery service and simultaneously to reduce operational costs. This paper presents a case of anticipatory shipping in the context of agro-food supply chains. The challenge in these chains is the product perishability that leads to product obsolescence in the case of un-balanced supply and demand. This study introduces a data-driven approach that integrates product quality characteristics in data analytics to identify suitable products for anticipatory shipping at the strategic level. It also proposes process redesigns concerning production and transportation at the operational level to realise anticipatory shipping. Finally, using historical data from a Dutch floriculture supplier as input for a multi-agent simulation, the proposed approach and process redesigns are verified. The simulation output shows that anticipatory shipping could increase delivery service level up to 35.3% and reduce associated costs up to 9.3%.

    Health effects of dietary risks in 195 countries, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017
    Afshin, Ashkan ; Sur, Patrick John ; Fay, Kairsten A. ; Cornaby, Leslie ; Ferrara, Giannina ; Salama, Joseph S. ; Mullany, Erin C. ; Abate, Kalkidan Hassen ; Abbafati, Cristiana ; Abebe, Zegeye ; Afarideh, Mohsen ; Aggarwal, Anju ; Agrawal, Sutapa ; Akinyemiju, Tomi ; Alahdab, Fares ; Bacha, Umar ; Bachman, Victoria F. ; Badali, Hamid ; Badawi, Alaa ; Bensenor, Isabela M. ; Bernabe, Eduardo ; Biadgilign, Sibhatu Kassa K. ; Biryukov, Stan H. ; Cahill, Leah E. ; Carrero, Juan J. ; Cercy, Kelly M. ; Dandona, Lalit ; Dandona, Rakhi ; Dang, Anh Kim ; Degefa, Meaza Girma ; Sayed Zaki, Maysaa El; Esteghamati, Alireza ; Esteghamati, Sadaf ; Fanzo, Jessica ; Sá Farinha, Carla Sofia e; Farvid, Maryam S. ; Farzadfar, Farshad ; Feigin, Valery L. ; Fernandes, Joao C. ; Flor, Luisa Sorio ; Foigt, Nataliya A. ; Forouzanfar, Mohammad H. ; Ganji, Morsaleh ; Geleijnse, Johanna M. ; Gillum, Richard F. ; Goulart, Alessandra C. ; Grosso, Giuseppe ; Guessous, Idris ; Hamidi, Samer ; Nguyen, Cuong Tat - \ 2019
    The Lancet 393 (2019)10184. - ISSN 0140-6736 - p. 1958 - 1972.

    Background: Suboptimal diet is an important preventable risk factor for non-communicable diseases (NCDs); however, its impact on the burden of NCDs has not been systematically evaluated. This study aimed to evaluate the consumption of major foods and nutrients across 195 countries and to quantify the impact of their suboptimal intake on NCD mortality and morbidity. Methods: By use of a comparative risk assessment approach, we estimated the proportion of disease-specific burden attributable to each dietary risk factor (also referred to as population attributable fraction)among adults aged 25 years or older. The main inputs to this analysis included the intake of each dietary factor, the effect size of the dietary factor on disease endpoint, and the level of intake associated with the lowest risk of mortality. Then, by use of disease-specific population attributable fractions, mortality, and disability-adjusted life-years (DALYs), we calculated the number of deaths and DALYs attributable to diet for each disease outcome. Findings: In 2017, 11 million (95% uncertainty interval [UI]10–12)deaths and 255 million (234–274)DALYs were attributable to dietary risk factors. High intake of sodium (3 million [1–5]deaths and 70 million [34–118]DALYs), low intake of whole grains (3 million [2–4]deaths and 82 million [59–109]DALYs), and low intake of fruits (2 million [1–4]deaths and 65 million [41–92]DALYs)were the leading dietary risk factors for deaths and DALYs globally and in many countries. Dietary data were from mixed sources and were not available for all countries, increasing the statistical uncertainty of our estimates. Interpretation: This study provides a comprehensive picture of the potential impact of suboptimal diet on NCD mortality and morbidity, highlighting the need for improving diet across nations. Our findings will inform implementation of evidence-based dietary interventions and provide a platform for evaluation of their impact on human health annually. Funding: Bill & Melinda Gates Foundation.

    The Impact of Credit Policy on Rice Production in Myanmar
    Aung, Nilar ; Nguyen, Hoa-Thi-Minh ; Sparrow, Robert - \ 2019
    Journal of Agricultural Economics 70 (2019)2. - ISSN 0021-857X - p. 426 - 451.
    Rural finance has long been an important tool for poverty reduction and rural development by donors and governments, but the impacts have been controversial. Measuring impact is challenging due to identification problems caused by selection bias and governments’ targeted interventions, while randomised trial data are scarce and limited to contexts where little to no rural finance exists. Using an author‐collected dataset, we provide insights on a large‐scale long‐lasting subsidised rice credit programme in Myanmar, one of the poorest and, until recently, most economically isolated countries in Asia. Identification relies on a fuzzy regression discontinuity design, exploiting an arbitrary element to the credit provision rule which is based on rice landholding size. Although we find little evidence that rice yield or output is increased, we do see that the programme has some positive effects on total household income, suggesting a positive spillover effect on other farm income activities.
    Kan automatische beeldherkenning helpen bij betere vangstregistratie? : Kunstmatige intelligentie
    Nguyen, Linh ; Helmond, A.T.M. van; Kootstra, G.W. - \ 2019
    Visserijnieuws (2019). - ISSN
    Global, regional, and national burden of neurological disorders, 1990–2016: a systematic analysis for the Global Burden of Disease Study 2016
    Feigin, Valery L. ; Nichols, Emma ; Alam, Tahiya ; Bannick, Marlena S. ; Beghi, Ettore ; Blake, Natacha ; Culpepper, William J. ; Dorsey, E.R. ; Elbaz, Alexis ; Ellenbogen, Richard G. ; Fisher, James L. ; Fitzmaurice, Christina ; Giussani, Giorgia ; Glennie, Linda ; James, Spencer L. ; Johnson, Catherine Owens ; Kassebaum, Nicholas J. ; Logroscino, Giancarlo ; Marin, Benoît ; Mountjoy-Venning, W.C. ; Nguyen, Minh ; Ofori-Asenso, Richard ; Patel, Anoop P. ; Piccininni, Marco ; Roth, Gregory A. ; Steiner, Timothy J. ; Stovner, Lars Jacob ; Szoeke, Cassandra E.I. ; Theadom, Alice ; Vollset, Stein Emil ; Wallin, Mitchell Taylor ; Wright, Claire ; Zunt, Joseph Raymond ; Abbasi, Nooshin ; Abd-Allah, Foad ; Abdelalim, Ahmed ; Abdollahpour, Ibrahim ; Aboyans, Victor ; Abraha, Haftom Niguse ; Acharya, Dilaram ; Adamu, Abdu A. ; Adebayo, Oladimeji M. ; Adeoye, Abiodun Moshood ; Adsuar, Jose C. ; Afarideh, Mohsen ; Agrawal, Sutapa ; Ahmadi, Alireza ; Ahmed, Muktar Beshir ; Aichour, Amani Nidhal ; Geleijnse, Johanna M. - \ 2019
    The Lancet Neurology 18 (2019)5. - ISSN 1474-4422 - p. 459 - 480.

    Background: Neurological disorders are increasingly recognised as major causes of death and disability worldwide. The aim of this analysis from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2016 is to provide the most comprehensive and up-to-date estimates of the global, regional, and national burden from neurological disorders. Methods: We estimated prevalence, incidence, deaths, and disability-adjusted life-years (DALYs; the sum of years of life lost [YLLs] and years lived with disability [YLDs]) by age and sex for 15 neurological disorder categories (tetanus, meningitis, encephalitis, stroke, brain and other CNS cancers, traumatic brain injury, spinal cord injury, Alzheimer's disease and other dementias, Parkinson's disease, multiple sclerosis, motor neuron diseases, idiopathic epilepsy, migraine, tension-type headache, and a residual category for other less common neurological disorders) in 195 countries from 1990 to 2016. DisMod-MR 2.1, a Bayesian meta-regression tool, was the main method of estimation of prevalence and incidence, and the Cause of Death Ensemble model (CODEm) was used for mortality estimation. We quantified the contribution of 84 risks and combinations of risk to the disease estimates for the 15 neurological disorder categories using the GBD comparative risk assessment approach. Findings: Globally, in 2016, neurological disorders were the leading cause of DALYs (276 million [95% UI 247–308]) and second leading cause of deaths (9·0 million [8·8–9·4]). The absolute number of deaths and DALYs from all neurological disorders combined increased (deaths by 39% [34–44] and DALYs by 15% [9–21]) whereas their age-standardised rates decreased (deaths by 28% [26–30] and DALYs by 27% [24–31]) between 1990 and 2016. The only neurological disorders that had a decrease in rates and absolute numbers of deaths and DALYs were tetanus, meningitis, and encephalitis. The four largest contributors of neurological DALYs were stroke (42·2% [38·6–46·1]), migraine (16·3% [11·7–20·8]), Alzheimer's and other dementias (10·4% [9·0–12·1]), and meningitis (7·9% [6·6–10·4]). For the combined neurological disorders, age-standardised DALY rates were significantly higher in males than in females (male-to-female ratio 1·12 [1·05–1·20]), but migraine, multiple sclerosis, and tension-type headache were more common and caused more burden in females, with male-to-female ratios of less than 0·7. The 84 risks quantified in GBD explain less than 10% of neurological disorder DALY burdens, except stroke, for which 88·8% (86·5–90·9) of DALYs are attributable to risk factors, and to a lesser extent Alzheimer's disease and other dementias (22·3% [11·8–35·1] of DALYs are risk attributable) and idiopathic epilepsy (14·1% [10·8–17·5] of DALYs are risk attributable). Interpretation: Globally, the burden of neurological disorders, as measured by the absolute number of DALYs, continues to increase. As populations are growing and ageing, and the prevalence of major disabling neurological disorders steeply increases with age, governments will face increasing demand for treatment, rehabilitation, and support services for neurological disorders. The scarcity of established modifiable risks for most of the neurological burden demonstrates that new knowledge is required to develop effective prevention and treatment strategies. Funding: Bill & Melinda Gates Foundation.

    What Is the Right Delivery Option for You? Consumer Preferences for Delivery Attributes in Online Retailing
    Nguyen, Dung H. ; Leeuw, Sander de; Dullaert, Wout ; Foubert, Bram P.J. - \ 2019
    Journal of Business Logistics 40 (2019)4. - ISSN 0735-3766 - p. 299 - 321.
    conjoint analysis - consumer behavior - E-commerce logistics - last mile delivery - mental accounting theory - online retail

    Nowadays, online retailers are offering a variety of delivery options consisting of varying combinations of delivery attributes. This study investigates how consumers value these delivery attributes (e.g., delivery speed, time slot, daytime/evening delivery, delivery date, and delivery fee) when selecting a delivery option for their online purchases. Mental accounting theory is used to frame the research and to suggest how mental accounts for money, time, and convenience influence consumer preferences for online delivery options. Specifically, the results of a conjoint analysis show that the most important attribute in shaping consumer preferences is the delivery fee, followed by nonprice delivery attributes. For individual attributes, significant differences are found in consumer preferences between gender and income groups. Cluster analysis reveals three consumer segments that show distinct preference structures: We identify a “price-oriented,” a “time- and convenience-oriented,” and a “value-for-money-oriented” consumer segment. This study has practical implications for online retailers when implementing suitable delivery strategies and designing effective delivery options to maximize consumer satisfaction.

    The impact of information sharing on the performance of horizontal logistics collaboration: A simulation study in an agri-food supply chain
    Yuan, Yaxing ; Viet, Nguyen ; Behdani, Behzad - \ 2019
    IFAC-PapersOnLine 52 (2019)13. - ISSN 2405-8963 - p. 2722 - 2727.
    Agri-food supply chain - Horizontal logistics collaboration - Information sharing - Optimization methods - Simulation tools - Supply chain management - Supply chains and networks

    In this paper, we study the value of horizontal logistics collaboration (HLC) for a case of Dutch horticultural supply chain. In order to deliver products on time, and hence, reach the desired service level, growers tend to carry out many trips in one day with low truck utilization. This situation can result in higher transportation cost, more pollution as well as low asset utilization. Collaborative logistics can be a potential strategy to cope with this situation. A simulation model is developed and the potential influencing factors the performance of HLC are studied. The results show that higher information sharing frequency can improve the performance by reducing the travel distance and improving service level and truck utilization. Moreover, the comparison of the performance with and without collaboration shows that that the growers with higher order frequency would potentially benefit more from an HLC setting

    Effect of inoculants of lactic acid bacteria on the fermentation quality of ryegrass at different dry matter content
    Huyen, Nguyen Thi ; Martinez, I. ; Pellikaan, W.F. - \ 2019
    In: Proceedings of the Congress on Gastrointestinal Function. - Chicago, Illinois : - p. 45 - 46.
    Effect of using fungal treated rice straw in sheep diet on nutrients digestibility and microbial protein synthesis
    Huyen, Nguyen Thi ; Tuan, Bui Quang ; Ngo, Xuan Nghien ; Nguyen, Thi Bich Thuy ; Le, Nguyen Thi Tuyet - \ 2019
    Asian Journal of Animal Sciences 13 (2019)1. - ISSN 1819-1878 - p. 1 - 7.
    Background and Objective: Biological methods by using white-rot fungi is one of the viable alternatives to improve the nutritional value of rice straw. Moreover, biological method is environmental friendly and potentially economic. The objective of this study was to evaluate the effect of utilisation of fungal treated rice straw in the diet of sheep on feed intake and nutrient digestibility when compared to untreated or urea treated rice straw. Materials and Methods: A total of 12 male Phan Rang sheep with body weight (20.58±1.16 kg) were randomly assigned to either a Rice Straw (RS) or a urea treated rice straw (UTR) or fugal treated rice straw (FTR) diet over an experimental period of 20 days in a completely randomized design. The RS diet was a mixture of rice straw, guinea grass and concentrates. In the UTR and the FTR diets, rice straw was replaced by urea treated rice straw and fungal treated rice straw, respectively. During 14 days of adaptation, all sheep were kept in individual pens and received their assigned diets ad libitum. During the following 6 days, the sheep were offered at 95% of ad libitum intake and moved to metabolism crates for total urine and fecal collection to evaluate feed intake, nutrients digestibility and N balance. All data were tested by ANOVA using the general model procedure of SAS. Results: The nutrient intake did not differ among three diets. However, apparent nutrient digestibility were highest (p<0.05) in sheep fed the FTR diet. The nitrogen retention and microbial protein synthesis were also greatest (4.32 and 6.04 g/day, respectively, p<0.05) for sheep fed the FTR diet. Conclusion: These results suggest that using fungal treated rice straw in the diet of sheep showed the best improvement of nutrient digestibility, N retention and microbial protein synthesis.
    Trypanosome-host interaction revealed through the zebrafish looking glass
    Jacobs, S.H. ; Doro, E. ; Hammond, Ffion ; Brugman, S. ; Nguyen-Chi, Mai ; Wiegertjes, G. ; Forlenza, M. - \ 2019
    Fish and Shellfish Immunology 91 (2019). - ISSN 1050-4648 - p. 443 - 443.
    Trypanosoma carassii is an extracellular blood parasite of cyprinid fish phylogenetically closely related to Trypanosoma brucei, the causative agent of the sleeping sickness disease in humans and livestock. Motility is crucial for trypanosome pathogenicity, but real-time visualization of parasite movement in vivo, in the natural host environment, has not been reported thus far. In this study, we report the establishment of T. carassii infection in zebrafish (Danio rerio), which allowed us, for the first time in a vertebrate host, to characterize in details the movement of trypanosomes in vivo. By combining the transparency of zebrafish larvae with the availability of several transgenic lines marking macrophages, neutrophils, cytokine-expressing leukocytes and endothelial cells, we were able to study in real-time: 1) parasite movement in vivo; 2) the kinetics of innate immune responses; and 3) parasite interaction with host (immune) cells. Our results indicate that during T. carassii infection of young zebrafish a differential macrophages and neutrophils response is observed. Macrophages responded more prominently than neutrophils by proliferating, and were massively recruited to blood vessels. Macrophages also exhibited heterogeneous morphologies and a strong pro-inflammatory profile. In fact, they were strongly positive for Tnfα and Il-1β and had a morphology characteristic of foamy macrophages. Large foamy macrophages accumulated in the portal vein of highly infected individuals, and were strongly positive for lipid staining, which revealed the abundance of lipid bodies in their cytoplasm. Finally, with respect to parasite movement and interaction with the host, using high-speed videography, we were able to capture novel mechanisms of parasite-host cell interaction, and to follow the onset of anemia, vasodilation and extravasation typical of trypanosome infections. Altogether, this is the first report of an in vivo trypanosome infection model in a natural vertebrate host describing both, the pathogen behavior and the host response. Considering that trypanosomes can infect all vertebrates, including humans, livestock and fish, our infection model is a relevant complementary tool to gain more insights in the underlying mechanisms of trypanosome infections.
    Using board games to raise awareness and skills for sustainable shrimp farming in Vietnam
    Tran Thi Phung, H. ; Nguyen, Thi Phuong ; Tong Quoc, Hiep ; Bosma, R.H. ; Ligtenberg, A. ; Rodela, R. ; Bregt, A.K. - \ 2019
    In: Book of abstracts of the 12th Asian Fisheries & Aquaculture Forum (AFAF). - - p. 321 - 321.
    Along its coast, the Vietnamese Mekong Delta has protected mangrove forests andbehind this a buffer zone of Integrated Mangrove Shrimp farms (IMS). Voluntaryadoption of IMS beyond the buffer zone is hampered by assumptions of farmersand policy-makers about profits compared to intensive shrimp monoculture.ALEGAMS developed a board-game allowing farmers to experience the financialconsequences of their decisions in shrimp farming, and assessed the effect ofplaying the board-game on farmer's learning and willingness to adopt IMS. Theplayers stated to have learned about Investment 8, operation costs, Management& technologies, IMS' profits, and risks of Intensive shrimp farming. Several monthafter having played 1-3 sessions, farmer's behavior had changed: slightly higherfrequency of weekly consultations and more persons consulted on practices &diseases, but increased suspicion about veterinary salesman, and technologytake-up after one training increased from 30 to 43%
    Using board games to raise awareness and skills for sustainable shrimp farming in Vietnam
    Tran Thi Phung, Ha ; Nguyen, Thi Phuong ; Tong Quoc, Hiep ; Bosma, Roel ; Ligtenberg, Arend ; Rodela, Romina ; Bregt, Arnold - \ 2019
    Along its coast, the Vietnamese Mekong Delta has protected mangrove forests and behind this a buffer zone of Integrated Mangrove Shrimp farms (IMS). Voluntary adoption of IMS beyond the buffer zone is hampered by assumptions of farmers and policy-makers about profits compared to intensive shrimp monoculture. ALEGAMS developed a board-game allowing farmers to experience the financial consequences of their decisions in shrimp farming, and assessed the effect of playing the board-game on farmer's learning and willingness to adopt IMS. The players stated to have learned about Investment 8, operation costs, Management & technologies, IMS' profits, and risks of Intensive shrimp farming. Several month after having played 1-3 sessions, farmer's behavior had changed: slightly higher frequency of weekly consultations and more persons consulted on practices & diseases, but increased suspicion about veterinary salesman, and technology take-up after one training increased from 30 to 43%
    Improving data collection for rays
    Nguyen, Linh ; Helmond, Edwin van; Batsleer, Jurgen ; Poos, Jan Jaap ; Kootstra, Gert - \ 2019
    Determining Key Research Areas for Healthier Diets and Sustainable Food Systems in Viet Nam
    Raneri, Jessica E. ; Kennedy, Gina ; Nguyen, Trang ; Wertheim-Heck, S.C.O. ; Haan, Stef de; Do, Ha Thi Phuong ; Nguyen, Phuong Hong ; Thi, Huong Le ; Mai, Truong Tuyet ; Duong, Thi Thanh Thuy ; Hung, Nguyen ; Nguyen, Tuan ; Huynh, Tuyen ; Nodari, Gulia Rota ; Spellman, Olga ; Talsma, Elise F. ; Stoian, Dietmar ; Duong, Minh-Cam ; Tran, Lam Nguyen ; Bene, Christophe - \ 2019
    IFPRI (IFPRI Discussion Paper 1872) - 127 p.
    food systems - diet - nutrition - agriculture
    Vietnamese food systems are undergoing rapid transformation, with important implications for human and environmental health and economic development. Poverty has decreased, and diet quality and under-nutrition have improved significantly since the end of the Doi Moi reform period (1986-1993) as a result of Viet Nam opening its economy and increasing its regional and global trade. Yet poor diet quality is still contributing the triple burden of malnutrition, with 25 percent stunting among children under age 5, 26 percent and 29 percent of women and children, respectively, anemic, and 21 percent of adults overweight. Agricultural production systems have shifted from predominantly diverse smallholder systems to larger more commercialized and specialized systems, especially for crops, while the ‘meatification’ of the Vietnamese diet is generating serious trade-offs between improved nutrition and sustainability of the Vietnamese food systems. The food processing industry has developed rapidly, together with food imports, resulting in new and processed food products penetrating the food retail outlets, trending towards an increase in the Westernized consumption patterns that are shifting nutrition-related problems towards overweight and obesity and, with it, an increase of non-communicable disease-related health risks. While regulatory policies exist across the food system, these are not systematically implemented, making food safety a major concern for consumers and policy makers alike. Where data exists, it is not easy to aggregate with data from across food system dimensions, making it difficult for Viet Nam to make an informed analysis of current and potential food system trade-offs. In our research, we reviewed existing literature and data, and applied a food systems framework to develop an initial food systems profile for Viet Nam and to identify a comprehensive set a of research questions to fill current data gaps identified through the review. Insights on these would provide the comprehensive evidence needed to inform policy makers on how to develop new food systems policies for Viet Nam, and further refine and improve existing policies to achieve better quality diets and more sustainable food systems in Viet Nam. Based on these, we then engaged with stakeholders to develop research priorities in the Viet Nam context and identified 25 priority research questions. This paper aims to stimulate such reflections by clearly outlining key areas for research, government policy, and development programs on priority investment to build the evidence base around inclusive food systems interventions that aim to result in healthier diets and more sustainable food systems for Viet Nam.
    Genome Improvement and Genetic Map Construction for Aethionema arabicum, the First Divergent Branch in the Brassicaceae Family.
    Nguyen, T.P. ; Mülich, Cornelia ; Mohammadin, Setareh ; Bergh, E. van den; Platts, A.E. ; Haas, Fabian B. ; Rensing, Stefan A. ; Schranz, M.E. - \ 2019
    Genes, Genomes and Genomics 9 (2019)11. - ISSN 1749-0383 - p. 3521 - 3530.
    The genus Aethionema is a sister-group to the core-group of the Brassicaceae family that includes Arabidopsis thaliana and the Brassica crops. Thus, Aethionema is phylogenetically well-placed for the investigation and understanding of genome and trait evolution across the family. We aimed to improve the quality of the reference genome draft version of the annual species Aethionema arabicum. Second, we constructed the first Ae. arabicum genetic map. The improved reference genome and genetic map enabled the development of each other. We started with the initially published genome (version 2.5). PacBio and MinION sequencing together with genetic map v2.5 were incorporated to produce the new reference genome v3.0. The improved genome contains 203 MB of sequence, with approximately 94% of the assembly made up of called (non-gap) bases, assembled into 2,883 scaffolds (with only 6% of the genome made up of non-called bases (Ns)). The N50 (10.3 MB) represents an 80-fold increase over the initial genome release. We generated a Recombinant Inbred Line (RIL) population that was derived from two ecotypes: Cyprus and Turkey (the reference genotype. Using a Genotyping by Sequencing (GBS) approach, we generated a high-density genetic map with 749 (v2.5) and then 632 SNPs (v3.0) was generated. The genetic map and reference genome were integrated, thus greatly improving the scaffolding of the reference genome into 11 linkage groups. We show that long-read sequencing data and genetics are complementary, resulting in an improved genome assembly in Ae. arabicum. They will facilitate comparative genetic mapping work for the Brassicaceae family and are also valuable resources to investigate wide range of life history traits in Aethionema.
    Mutagenesis of odorant coreceptor Orco fully disrupts foraging but not oviposition behaviors in the hawkmoth Manduca sexta
    Fandino, Richard A. ; Alexander, H. ; Bisch-Knaden, S. ; Zhang, J. ; Bucks, S. ; Nguyen, T.A.T. ; Schröder, K. ; Werckenthin, Achim ; Rybak, J. ; Stengl, Monika ; Knaden, M. ; Hansson, Bill S. ; Groβe-Wilde, Ewald - \ 2019
    Proceedings of the National Academy of Sciences of the United States of America 116 (2019)31. - ISSN 0027-8424 - p. 15677 - 15685.
    CRISPR-Cas9 - Insect olfaction - Insect-plant interactions - Manduca sexta - Orco

    The hawkmoth Manduca sexta and one of its preferred hosts in the North American Southwest, Datura wrightii, share a model insect-plant relationship based on mutualistic and antagonistic life-history traits. D. wrightii is the innately preferred nectar source and oviposition host for M. sexta. Hence, the hawkmoth is an important pollinator while the M. sexta larvae are specialized herbivores of the plant. Olfactory detection of plant volatiles plays a crucial role in the behavior of the hawkmoth. In vivo, the odorant receptor coreceptor (Orco) is an obligatory component for the function of odorant receptors (ORs), a major receptor family involved in insect olfaction. We used CRISPR-Cas9 targeted mutagenesis to knock out (KO) the MsexOrco gene to test the consequences of a loss of OR-mediated olfaction in an insect-plant relationship. Neurophysiological characterization revealed severely reduced antennal and antennal lobe responses to representative odorants emitted by D. wrightii. In a wind-tunnel setting with a flowering plant, Orco KO hawkmoths showed disrupted flight orientation and an ablated proboscis extension response to the natural stimulus. The Orco KO gravid female displayed reduced attraction toward a nonflowering plant. However, more than half of hawkmoths were able to use characteristic odor-directed flight orientation and oviposit on the host plant. Overall, OR-mediated olfaction is essential for foraging and pollination behaviors, but plant-seeking and oviposition behaviors are sustained through additional OR-independent sensory cues.

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