Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    The genetic and functional analysis of flavor in commercial tomato: the FLORAL4 gene underlies a QTL for floral aroma volatiles in tomato fruit
    Tikunov, Yury M. ; Roohanitaziani, Raana ; Meijer-Dekens, Fien ; Molthoff, Jos ; Paulo, Joao ; Finkers, Richard ; Capel, Iris ; Carvajal Moreno, Fatima ; Maliepaard, Chris ; Nijenhuis-de Vries, Mariska ; Labrie, Caroline W. ; Verkerke, Wouter ; Heusden, Adriaan W. van; Eeuwijk, Fred van; Visser, Richard G.F. ; Bovy, Arnaud G. - \ 2020
    The Plant Journal 103 (2020)3. - ISSN 0960-7412
    2-phenylethanol - aroma - flavor - quantitative trait loci - Solanum lycopersicum - tomato - volatiles

    Tomato (Solanum lycopersicum L.) has become a popular model for genetic studies of fruit flavor in the last two decades. In this article we present a study of tomato fruit flavor, including an analysis of the genetic, metabolic and sensorial variation of a collection of contemporary commercial glasshouse tomato cultivars, followed by a validation of the associations found by quantitative trait locus (QTL) analysis of representative biparental segregating populations. This led to the identification of the major sensorial and chemical components determining fruit flavor variation and detection of the underlying QTLs. The high representation of QTL haplotypes in the breeders’ germplasm suggests that there is great potential for applying these QTLs in current breeding programs aimed at improving tomato flavor. A QTL on chromosome 4 was found to affect the levels of the phenylalanine-derived volatiles (PHEVs) 2-phenylethanol, phenylacetaldehyde and 1-nitro-2-phenylethane. Fruits of near-isogenic lines contrasting for this locus and in the composition of PHEVs significantly differed in the perception of fruity and rose-hip-like aroma. The PHEV locus was fine mapped, which allowed for the identification of FLORAL4 as a candidate gene for PHEV regulation. Using a gene-editing-based (CRISPR-CAS9) reverse-genetics approach, FLORAL4 was demonstrated to be the key factor in this QTL affecting PHEV accumulation in tomato fruit.

    Metagenomic-and cultivation-based exploration of anaerobic chloroform biotransformation in hypersaline sediments as natural source of chloromethanes
    Peng, Peng ; Lu, Yue ; Bosma, Tom N.P. ; Nijenhuis, Ivonne ; Nijsse, Bart ; Shetty, Sudarshan A. ; Ruecker, Alexander ; Umanets, Alexander ; Ramiro-Garcia, Javier ; Kappler, Andreas ; Sipkema, Detmer ; Smidt, Hauke ; Atashgahi, Siavash - \ 2020
    Microorganisms 8 (2020)5. - ISSN 2076-2607
    Biotransformation - Chloroform - Hypersaline lakes - Metagenome

    Chloroform (CF) is an environmental contaminant that can be naturally formed in various environments ranging from forest soils to salt lakes. Here we investigated CF removal potential in sediments obtained from hypersaline lakes in Western Australia. Reductive dechlorination of CF to dichloromethane (DCM) was observed in enrichment cultures derived from sediments of Lake Strawbridge, which has been reported as a natural source of CF. No CF removal was observed in abiotic control cultures without artificial electron donors, indicating biotic CF dechlorination in the enrichment cultures. Increasing vitamin B12 concentration from 0.04 to 4 µM in enrichment cultures enhanced CF removal and reduced DCM formation. In cultures amended with 4 µM vitamin B12 and13C labelled CF, formation of13CO2 was detected. Known organohalide-respiring bacteria and reductive dehalogenase genes were neither detected using quantitative PCR nor metagenomic analysis of the enrichment cultures. Rather, members of the order Clostridiales, known to co-metabolically transform CF to DCM and CO2, were detected. Accordingly, metagenome-assembled genomes of Clostridiales encoded enzymatic repertoires for the Wood-Ljungdahl pathway and cobalamin biosynthesis, which are known to be involved in fortuitous and nonspecific CF transformation. This study indicates that hypersaline lake microbiomes may act as a filter to reduce CF emission to the atmosphere.

    Metagenomic survey of chloroform fate in hypersaline lake Strawbridge in western Australia
    Peng, Peng ; Lu, Yue ; Bosma, Tom ; Nijenhuis, Ivonne ; Nijsse, Bart ; Shetty, Sudarshan ; Ruecker, Alexander ; Umanetc, Alexander ; Ramiro Garcia, Javier ; Kappler, Andreas ; Sipkema, Detmer ; Smidt, Hauke ; Atashgahi, Siavash - \ 2019
    Wageningen University
    PRJEB32090 - ERP114723 - Chloroform degradation - Hypersaline lakes - Sediment metagenome
    Chloroform (CF) is an environmental contaminant that can be naturally formed in hypersaline lake Strawbridge in western Australia. Anaerobic enrichment cultures using sediments from this lake showed CF degradation to dichloromethane (DCM) and CO2. The potential microbes and genes involved in CF degradation were investigated using metagenome sequencing. Known organohalide-respiring bacteria and their reductive dehalogenase genes were not detected. Rather, acetogens like Clostridium and methanogens like Methanosarcina that are capable for co-metabolically degrading CF to DCM and CO2 were detected in the sediment microcosms and enrichment cultures. Additionally, haloalkane dehalogenase and oxygenase that are possibly involved in further aerobic degradation of DCM were also detected in the sediment microcosms and enrichment cultures. These findings show that microbiota may act as a filter for CF emission from hypersaline lakes to the atmosphere.
    A validated survey to measure household food waste
    Herpen, Erica van; Geffen, Lisanne van; Nijenhuis-de Vries, Mariska ; Holthuysen, Nancy ; Lans, Ivo van der; Quested, Tom - \ 2019
    MethodsX 6 (2019). - ISSN 2215-0161 - p. 2767 - 2775.
    Consumer - Food waste - Household food waste questionnaire - Measurement - Questionnaire

    To assess household food waste in large-scale studies with the aim to understand differences in food waste levels between households, surveys are often employed. Yet, survey measures rely on people's awareness of their own food waste levels, draw upon their memory of instances of food waste, and can be subject to social desirability. Therefore, existing survey measures may not be optimal in measuring household food waste. The Household Food Waste Questionnaire has been developed to ameliorate these issues. It uses a pre-announcement to increase awareness of food waste, focuses on a short and specific time period (i.e. the past week), and specifies detailed product categories, whereas previous surveys mostly used general questions without reference to time period or product category. The amount of household food waste estimated using the Household Food Waste Questionnaire is likely to considerably underestimate the actual amount, so the method should not be used to obtain accurate waste amounts, but rather to distinguish differences between households and/or across time. Advantages compared to existing survey methods are that it: • Distinguishes better between households with low versus high levels of food waste • Correlates more highly with other measurements of household food waste (diary, kitchen caddy, photograph coding)

    Postharvest quality of different papaya cultivars : GreenCHAINge Fruit & Vegetables WP2
    Brouwer, Bastiaan ; Nijenhuis-de Vries, Mariska ; Pereira da Silva, Fátima I. ; Harchioui, Najim El ; Gabriëls, Suzan - \ 2019
    Wageningen : Wageningen Food & Biobased Research (Rapport / Wageningen Food & Biobased Research 1916) - 40
    Tools om uitstalleven van Conference peren voor verre bestemmingen te verbeteren : GreenCHAINge, G&F, DP5, BO-29.03-001-010
    Hogeveen-van Echtelt, Esther ; Paillart, Maxence ; Wild, Hans de; Nijenhuis, Mariska - \ 2019
    Wageningen : Wageningen Food & Biobased Research (Rapport / Wageningen Food & Biobased Research 1878) - 33
    Binnen het project GreenCHAINge G&F (DP5) wordt gewerkt aan het ontwikkelen verbeteren van kwaliteitsbeheersing van peren bestemd voor nieuwe, verre bestemmingen (o.a. China, Brazilië, Vietnam). Om deze langeafstandsmarkten succesvol te kunnen voorzien van optimale kwaliteit peren is gedurende 4 jaar nieuwe kennis opgedaan d.m.v. onderzoek. Er zijn experimenten uitgevoerd met behulp van keten- en transportsimulaties, waarbij diverse tools op verschillende momenten in de keten onderzocht zijn op hun effectiviteit en bruikbaarheid voor verbetering van de kwaliteit en uitstalleven in de langeafstandsketen. Dit rapport beschrijft de resultaten van deze experimenten. Samengevat tonen de resultaten aan dat van de verschillende tools die zijn onderzocht, met name toepassing van SmartFresh en een juiste toepassing van Modified Atmosphere (MA) verpakkingen (ook op bestemming) de grootste effecten hebben op het behoud van hardheid en vaak ook grondkleur. Andere methoden zoals Bio-Fresh, aangepaste koolzuur (CO2)- en/of zuurstof (O2) gehalten tijdens het koude transport, hebben minder tot geen effect. Naast de gangbare vroege toepassing van SmartFresh tijdens bewaring blijken er mogelijkheden te zijn voor toepassing van SmartFresh na bewaring of tijdens transport, mits een hogere dosering en/of langere behandelingsduur toegepast wordt. Een dergelijke toepassing vraagt verdere optimalisatie worden om consistente resultaten te bereiken in de praktijk. Ethyleenproductiemetingen bieden enige hulp, maar werken niet altijd goed voor voorspellingen van effectiviteit op partijbasis. Toepassing van SmartFresh aan het einde van bewaring of tijdens transport in hoge concentraties, vereist nog een toelating om in de praktijk gebruikt te mogen worden. De resultaten van het onderzoek met MA-verpakkingen zijn hoopgevend om in een relatief warme keten toch een behoud van hardheid en grondkleur te kunnen realiseren. Bij Wageningen Food & Biobased Research is een baanbrekend verpakkingsmateriaal ontwikkeld, gemaakt van een combinatie van hernieuwbaar (biobased) materiaal en conventioneel verpakkingsmateriaal. Dit folie met speciale eigenschappen met betrekking tot gasdoorlaatbaarheid, resulteerde in de experimenten in goed behoud van hardheid en kleur, terwijl het risico op inwendig bruin problemen in de peren beperkt werd doordat het CO2-gehalte in de verpakking niet te hoog opliep.
    Control of restrictive supply chains : biomarkers as indicator for Erwinia infection on potato tuber
    Paillart, M.J.M. ; Pereira da Silva, F.I.D.G. ; Nijenhuis-de Vries, M.A. ; Harchiou, N. El; Brouwer, B. ; Kekem, C. van - \ 2019
    Wageningen : Wageningen Food & Biobased Research (Report / Wageningen Food & Biobased Research 1947) - 33
    Comparing wasted apples and oranges : An assessment of methods to measure household food waste
    Herpen, Erica van; Lans, Ivo A. van der; Holthuysen, Nancy ; Nijenhuis-de Vries, Mariska ; Quested, Tom E. - \ 2019
    Waste Management 88 (2019). - ISSN 0956-053X - p. 71 - 84.
    Caddy - Consumer - Diary - Photo - Survey - Waste

    Food waste has become a global concern in recent years, especially the household food waste that is generated in the developed world. Multiple methods to measure household food waste have been proposed, but little is known about their validity. Five methods are selected and investigated empirically: survey questions about general food waste over a non-specified period of time, diaries, photo coding, kitchen caddies, and pre-announced survey questions regarding a specific time period. In an experiment, respondents were asked to assess their food waste using some or all of these methods depending on condition. Overall, the general survey questions appear to be less valid, as these lead to large underestimation of the level of food waste, low variance in reported food waste across households compared to the other methods, and low correlations with other measures. The other four methods are relatively highly correlated. A survey about food waste in the past week appears to be a useful method for large-scale measurements to differentiate households according to the amount of food waste each produces, although it should be noted that this method underestimates the amount of food waste. Kitchen caddies and photo coding seem to be valid methods and, for small samples, provide alternatives to food diaries, which have been more commonly used.

    Throwing out the apples and the oranges: A comparison of methods to measure in-home food waste
    Herpen, Erica van; Lans, Ivo van der; Holthuysen, Nancy ; Nijenhuis-de Vries, Mariska ; Quested, T. - \ 2018
    Throwing out the apples and the oranges: A comparison of methods to measure in-home food waste
    Herpen, Erica van; Lans, Ivo van der; Holthuysen, Nancy ; Nijenhuis, Mariska ; Quested, T. - \ 2018
    Groente en fruit tijdens het 10-uurtje op school : hoe kijken ouders hier tegen aan? : PPS Project: Groente en fruit op school vanzelfsprekend! : de rol van de ouders en school
    Zeinstra, Gertrude G. ; Nijenhuis-de Vries, Mariska ; Haveman-Nies, Annemien - \ 2018
    Wageningen : Wageningen Food & Biobased Research (Wageningen Food & Biobased Research rapport 1809) - ISBN 9789463438483 - 41
    Organohalide respiration in a pristine hypersaline lake
    Peng, Peng ; Lu, Yue ; Nijenhuis, Ivonne ; Nijsse, Bart ; Shetty, Sudarshan ; Ruecker, Alexander ; Umanetc, Alexander ; Ramiro Garcia, Javier ; Kappler, Andreas ; Sipkema, Detmer ; Smidt, Hauke ; Atashgahi, Siavash - \ 2017
    Wageningen University & Research
    PRJEB14107 - ERP015719 - Organohalide respiration - Hypersaline lake - uncultured bacterium
    Despite compiling evidence on distribution of organohalide-respiring bacteria (OHRB) in pristine environments, there is lack of knowledge about their occurrence and potential contribution to the chlorine cycle in extreme environments such as hypersaline lakes. Biotic formation of organohalides (OHs) such as chloromethane and chloroform was recently reported from the hypersaline Lake Strawbridge, Australia. Assuming that natural availability of OHs can prime development of organohalide respiration potential, we prepared microcosms from Lake Strawbridge sediments. Amended chloroform and tetrachloroethene (PCE) were stoichiometrically dechlorinated to dichloromethane and trichloroethene, respectively. MiSeq 16S ribosomal RNA gene analysis and quantitative PCR did not detect any known chloroform and PCE-dechlorinating OHRB in anaerobic sediment microcosms and transfers, which indicated the presence of novel OHRB in hypersaline Lake Strawbridge. To our knowledge, this is the first report on OHR occurrence in extreme pristine environments. Combined with the formerly documented biotic chlorination, this suggests interdependencies between microbial chlorinators and dechlorinators in the local chlorine cycle in an extreme ecosystem.
    Fresh, frozen, or ambient food equivalents and their impact on food waste generation in Dutch households
    Janssen, Anke M. ; Nijenhuis, Mariska ; Boer, Eric P.J. ; Kremer, Stefanie - \ 2017
    Waste Management 67 (2017). - ISSN 0956-053X - p. 298 - 307.
    Food disposal - Food preservation - Freezer - Meal planning - Product-specific - Waste index
    In Europe, it is estimated that more than 50% of total food waste - of which most is avoidable - is generated at household level. Little attention has been paid to the impact on food waste generation of consuming food products that differ in their method of food preservation. This exploratory study surveyed product-specific possible impacts of different methods of food preservation on food waste generation in Dutch households. To this end, a food waste index was calculated to enable relative comparisons of the amounts of food waste from the same type of foods with different preservation methods on an annual basis. The results show that, for the majority of frozen food equivalents, smaller amounts were wasted compared to their fresh or ambient equivalents. The waste index (WI) proposed in the current paper confirms the hypothesis that it may be possible to reduce the amount of food waste at household level by encouraging Dutch consumers to use (certain) foods more frequently in a frozen form (instead of fresh or ambient). However, before this approach can be scaled to population level, a more detailed understanding of the underlying behavioural causes with regard to food provisioning and handling and possible interactions is required.
    Consumption life cycle contributions : Assessment of practical methodologies for in home food waste measurement
    Herpen, H.W.I. van; Lans, I.A. van der; Nijenhuis, M.A. ; Holthuysen, N.T.E. ; Kremer, S. ; Stijnen, D.A.J.M. ; Geffen, E.J. van - \ 2016
    REFRESH - 131 p.
    Best practice measurement of household level food waste : Milestone no. 2
    Herpen, Erica van; Lans, I.A. van der; Nijenhuis, M.A. ; Holthuysen, N.T.E. ; Kremer, S. - \ 2016
    EU - 26 p.
    This report is part of the EU research project REFRESH, which aims to contribute to the goal of reducing food waste across Europe. The current report is output of the work package that focuses on consumer behaviours related to food waste. It aims to consolidate existing and new consumer understandings at the in-home level into a research framework and methodology that allows comparison across countries.
    Insights on older adults' perception of at-home sensory-hedonic methods : A case of Ideal Profile Method and CATA with ideal
    Ruark, Angelica ; Vingerhoeds, Monique H. ; Kremer, Stefanie ; Nijenhuis, Mariska ; Piqueras-Fiszman, Betina - \ 2016
    Food Quality and Preference 53 (2016). - ISSN 0950-3293 - p. 29 - 38.
    Aging - CATA - IPM - Older consumers - Protein - Sensory-hedonic methods

    The increasing average life expectancy and overall percentage of the older population is leading to a growing interest in healthy aging. Thus, there is a demand for flavourful, and pleasant foods, rich in certain nutrients, targeted at older consumers. In order to be able to tailor foods to older consumers wants and needs, it is necessary to investigate and - if necessary - to further improve sensory and consumer science testing methodologies when working with healthy and frail/malnourished older adults.This study aims to investigate older consumers' perception and performance of two sensory-hedonic methods conducted at home based on their ease of use, preference, duration to complete the task, and discrimination ability. Insights about their understanding of the tasks were also collected. Additionally, the study compares the older adults' performance and perception to those of young consumers. The two methods selected for comparison were Yes/No forced-choice Check-All-That-Apply with ideal (CATA-I) and Ideal Profile Method (IPM).Due to the interest in older consumers increasing their protein intake, seven protein-enriched dairy drinks were selected. The dairy drinks were sampled one per day, using one method one week and the other method two weeks later by 67 older (M = 67.96 y.o., SD = 5.09) and 83 young subjects (M = 22.95 y.o., SD = 2.31). Although the IPM method was reported to be more difficult for the older group than CATA-I, there was no preference between the two methods. In addition, they seemed to struggle with the limited options in CATA-I. The data of the older consumers revealed a similar number of discriminating attributes to characterise the products using both methods; the results were also similar to those of the young participants. This study provides novel consumer-centric insights on the use and understanding of two relatively new sensory-hedonic methods by older adults.

    Sensory quality of drinking water produced by reverse osmosis membrane filtration followed by remineralisation
    Vingerhoeds, M.H. ; Nijenhuis, M.A. ; Ruepert, N. ; Bredie, W.L.P. ; Kremer, S. - \ 2016
    Water Research 94 (2016). - ISSN 0043-1354 - p. 42 - 51.
    drinking water - water quality - sensory evaluation - taste research - reverse osmosis - membranes - filtration - drinkwater - waterkwaliteit - sensorische evaluatie - smaakonderzoek - omgekeerde osmose - membranen - filtratie
    Membrane filtration of ground, surface, or sea water by reverse osmosis results in permeate, which is almost free from minerals. Minerals may be added afterwards, not only to comply with (legal) standards and to enhance chemical stability, but also to improve the taste of drinking water made from permeate. Both the nature and the concentrations of added minerals affect the taste of the water and in turn its acceptance by consumers. The aim of this study was to examine differences in taste between various remineralised drinking waters. Samples selected varied in mineral composition, i.e. tap water, permeate, and permeate with added minerals (40 or 120 mg Ca/L, added as CaCO3, and 4 or 24 mg Mg/L added as MgCl2), as well as commercially available bottled drinking waters, to span a relevant product space in which the remineralised samples could be compared. All samples were analysed with respect to their physical–chemical properties. Sensory profiling was done by descriptive analysis using a trained panel. Significant attributes included taste intensity, the tastes bitter, sweet, salt, metal, fresh and dry mouthfeel, bitter and metal aftertaste, and rough afterfeel. Total dissolved solids (TDS) was a major determinant of the taste perception of water. In general, lowering mineral content in drinking water in the range examined (from <5 to 440 mg/L) shifted the sensory perception of water from fresh towards bitter, dry, and rough sensations. In addition, perceived freshness of the waters correlated positively with calcium concentration. The greatest fresh taste was found for water with a TDS between 190 and 350 mg/L. Remineralisation of water after reverse osmosis can improve drinking quality significantly.
    Foodbattle: vervolg & opschaling 2014
    Bos-Brouwers, H.E.J. ; Nijenhuis, M.A. ; Holthuysen, N.T.E. ; Hamersveld, Nick van; Burgh, Marianne van der; Brouwer, M.T. ; Scheer, F.P. - \ 2015
    Wageningen : Wageningen UR - Food & Biobased Research - ISBN 9789462575196
    An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans
    Suxian Zhu, Suxian ; Vossen, J.H. ; Bergervoet-van Deelen, J.E.M. ; Nijenhuis, M.A. ; Kodde, L.P. ; Kessel, G.J.T. ; Vleeshouwers, V.G.A.A. ; Visser, R.G.F. ; Jacobsen, E. - \ 2015
    Euphytica 202 (2015)2. - ISSN 0014-2336 - p. 219 - 234.
    resistance genes - solanum-bulbocastanum - disease resistance - durable resistance - rxlr effectors - united-states - pathogen - population - races - diversity
    Late blight is an important disease in potato that is caused by the oomycete Phytophthora infestans. In the past, Solanum demissum late blight resistance (R) genes were introgressed into cultivated potato (Solanum tuberosum). Eleven of these resistant plants were selected to characterize the virulence spectrum of individual P. infestans isolates and to monitor the dynamics of virulence in P. infestans populations. These plants are referred to as the Mastenbroek and Black differential sets. It has long been assumed that each differential plant contained one single R gene. In the current study and previous studies, however, most Mastenbroek differential plants were shown to harbor multiple R gene(s), which blurs virulence typing of late blight isolates. In order to acquire more accurate virulence profiles, we extended the Mastenbroek differential set with Solanum spp. plants harboring reduced R gene complexity and with plants containing recently identified R genes from related but different Solanum species. In addition, a differential set of ten Genetically Modified (GM) plants harboring single late blight R genes in the same genetic background (Desiree). By analyzing the virulence spectra of recently collected isolates using both newly described differential sets, we found that the GM Desiree differential set was more accurate for isolate virulence typing than the conventional (extended) differential set. Besides, the GM Desiree differential set was shown to be useful as trap plants to isolate novel P. infestans strains and to monitor virulence towards particular R genes in P. infestans populations `on site´. Legislative restrictions are, however, limiting the use of the GM Desiree differential set.
    Farmers on the move : mobility, access to land and conflict in Central and South Mali
    Nijenhuis, K. - \ 2013
    Wageningen University. Promotor(en): Han van Dijk, co-promotor(en): M.M.A. Kaag. - Wageningen : Wageningen UR - ISBN 9789461737205 - 290
    boeren - mobiliteit - politieke processen - landgebruik - grondrechten - landbouw bedrijven - conflict - toegang - land - mali - farmers - mobility - political processes - land use - land rights - farming - conflict - access - land - mali
    In contrast to their sedentary image, farmers in Central and South Mali are surprisingly mobile. Many have settled in scattered farming hamlets where they are rapidly expanding the areas under agriculture. This study focuses on farmers’ mobility in relation to accessing land in two regions in Mali where farming conditions are very different regarding rainfall, population growth and opportunities for income generation. It is shown that differences in farming conditions in the two regions have shaped the different temporal and spatial dimensions of farmers’ mobility. This mobility is, however, not just a reaction to changing farming conditions but also part of local political processes, including conflict, that mediate farmers’ access to land. By highlighting the crucial role of farming conditions and farmers’ mobility in these political processes, this study adds a fresh geographical dimension to ways of thinking about access to land, land use and conflict in West Africa and beyond.
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