Ig light chain variability in DNP494-KLH immunised sea bass (Dicentrarchus labrax L.) : evidence for intra-molecular induced suppression
Santos, N.M.S. dos; Hermsen, T. ; Rombout, J.H.W.M. ; Pilström, L. ; Stet, R.J.M. - \ 2001
Developmental and Comparative Immunology 25 (2001). - ISSN 0145-305X - p. 387 - 401.
The coding sequence of the sea bass light chain was obtained by sequential anchored PCR on a head kidney cDNA library of a DNP494-KLH immunised sea bass. The cDNA sequence obtained codes for a leader peptide of 21 aa and a mature IgL chain of 223 aa. Both the amino acid sequence comparisons and neighbour-joining trees showed that the IgL chain of sea bass obtained is of the L1/G type. To study the variability of the light chain, additional PCRs on the cDNA library and cDNA from pooled head kidneys were performed. Multiple alignment of unique sequences (N=17) could be performed without introducing gaps, and showed extremely low variability in CDR1, and no variability in CDR2 or CDR3. A possible explanation for this low variability of the IgL1 chain might be the enhanced expression of monospecific anti-DNP antibodies. The isolation and characterisation of partial genomic and cDNA IgL sequences, which showed normal variability, corroborate this explanation
|Major histocompatibility complex class I genes in Atlantic cod (Gadus morhua)
Persson, A.C. ; Stet, R.J.M. ; Pilström, L. - \ 2000
In: Developmental and Comparative Immunology : VIIIth ISDCI congress, Cairns Australia, 2000 - p. S82 - S82.
|Characterization of the immunoglobulin light chain of seabass (Dicentrachux labrax) suggests possible low diversity of the V region
Santos, N.M.S. dos; Hermsen, T. ; Pilstrom, L. ; Stet, R.J.M. - \ 2000
In: Developmental and Comparative Immunology : VIIIth ISDCI Congress, Cairns Australia, 2000 - p. S38 - S38.
|Characterisation of MHC class I and beta2-microglobulin sequences in Atlantic cod revealing an unusually high number of expressed class I genes
Persson, A.C. ; Stet, R.J.M. ; Pilström, L. - \ 1999
Immunogenetics 50 (1999). - ISSN 0093-7711 - p. 49 - 59.
Characterisation of beta2-microglobulin in a primitive fish the Siberian sturgeon Acipenser baeri
Lundqvist, M.L. ; Appelkvist, P. ; Hermsen, T. ; Pilstrom, P. ; Stet, R.J.M. - \ 1999
Immunogenetics 50 (1999)1-2. - ISSN 0093-7711 - p. 79 - 83.
|The complete MHC class I encoding genes in Atlantic cod (Gadus morhua L.).
Persson, A.C. ; Stet, R.J.M. ; Pilstrom, L. - \ 1997
Hereditas 127 (1997). - ISSN 0018-0661 - p. 166 - 166.