Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Records 1 - 6 / 6

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    Ig light chain variability in DNP494-KLH immunised sea bass (Dicentrarchus labrax L.) : evidence for intra-molecular induced suppression
    Santos, N.M.S. dos; Hermsen, T. ; Rombout, J.H.W.M. ; Pilström, L. ; Stet, R.J.M. - \ 2001
    Developmental and Comparative Immunology 25 (2001). - ISSN 0145-305X - p. 387 - 401.
    The coding sequence of the sea bass light chain was obtained by sequential anchored PCR on a head kidney cDNA library of a DNP494-KLH immunised sea bass. The cDNA sequence obtained codes for a leader peptide of 21 aa and a mature IgL chain of 223 aa. Both the amino acid sequence comparisons and neighbour-joining trees showed that the IgL chain of sea bass obtained is of the L1/G type. To study the variability of the light chain, additional PCRs on the cDNA library and cDNA from pooled head kidneys were performed. Multiple alignment of unique sequences (N=17) could be performed without introducing gaps, and showed extremely low variability in CDR1, and no variability in CDR2 or CDR3. A possible explanation for this low variability of the IgL1 chain might be the enhanced expression of monospecific anti-DNP antibodies. The isolation and characterisation of partial genomic and cDNA IgL sequences, which showed normal variability, corroborate this explanation
    Major histocompatibility complex class I genes in Atlantic cod (Gadus morhua)
    Persson, A.C. ; Stet, R.J.M. ; Pilström, L. - \ 2000
    In: Developmental and Comparative Immunology : VIIIth ISDCI congress, Cairns Australia, 2000 - p. S82 - S82.
    Characterization of the immunoglobulin light chain of seabass (Dicentrachux labrax) suggests possible low diversity of the V region
    Santos, N.M.S. dos; Hermsen, T. ; Pilstrom, L. ; Stet, R.J.M. - \ 2000
    In: Developmental and Comparative Immunology : VIIIth ISDCI Congress, Cairns Australia, 2000 - p. S38 - S38.
    Characterisation of MHC class I and beta2-microglobulin sequences in Atlantic cod revealing an unusually high number of expressed class I genes
    Persson, A.C. ; Stet, R.J.M. ; Pilström, L. - \ 1999
    Immunogenetics 50 (1999). - ISSN 0093-7711 - p. 49 - 59.
    Characterisation of beta2-microglobulin in a primitive fish the Siberian sturgeon Acipenser baeri
    Lundqvist, M.L. ; Appelkvist, P. ; Hermsen, T. ; Pilstrom, P. ; Stet, R.J.M. - \ 1999
    Immunogenetics 50 (1999)1-2. - ISSN 0093-7711 - p. 79 - 83.
    The complete MHC class I encoding genes in Atlantic cod (Gadus morhua L.).
    Persson, A.C. ; Stet, R.J.M. ; Pilstrom, L. - \ 1997
    Hereditas 127 (1997). - ISSN 0018-0661 - p. 166 - 166.
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