Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Validation of an ultra-high-performance liquid chromatography-mass spectrometry method for the quantification of cysteinylated aldehydes and application to malt and beer samples
    Bustillo Trueba, P. ; Jaskula-Goiris, B. ; Clippeleer, J. De; Goiris, K. ; Praet, T. ; Sharma, U.K. ; Eycken, E. Van der; Sanders, M.G. ; Vincken, J.P. ; Brabanter, J. De; Rouck, G. De; Aerts, G. ; Cooman, L. De - \ 2019
    Journal of Chromatography. A, Including electrophoresis and other separation methods 1604 (2019). - ISSN 0021-9673
    2-substituted 1,3-thiazolidine-4-carboxylic acids - Beer - Cysteinylated aldehydes - Liquid chromatography-mass spectrometry - Malt - Method validation

    This paper describes the method validation for the simultaneous determination of seven cysteinylated aldehydes, i.e. 2-substituted 1,3-thiazolidines-4-carboxylic acids, using ultra-high-performance liquid chromatography-mass spectrometry (UHPLC–MS). Authentic reference compounds were first synthesized for identification and quantification purposes. Moreover, nuclear magnetic resonance (1H NMR and 13C NMR) was applied for verification of their structure, while ultra-high-performance liquid chromatography–mass spectrometry (UHPLC–MS) was applied for estimation of the purity. The method for quantification of cysteinylated aldehydes in model solutions has been validated according to the criteria and procedures described in international standards. The synthesized compounds were successfully identified via UHPLC–MS by comparing retention time and MS spectra with the commercial reference compounds. Method validation revealed good linearity (R2 > 0.995) over the range of 0.4–2.2 µg/L to approximately 1000 µg/L, depending on the analyte. The limits of quantification varied from 0.9 to 4.3 µg/L depending on the nature of the compound. Furthermore, evaluation of the method showed good accuracy and stability of the standard solutions. Reported chromatographic recoveries ranged from 112 to 120%. Consequently, the currently described method was applied on malt and beer samples. For the first time, quantification of cysteinylated aldehydes was obtained in malt. In contrast, in fresh beers unambiguous identification of these compounds was not achieved.

    Estimation of hepatitis E virus (HEV) pig seroprevalence using Elisa and Western blot and comparison between human and pig HEV sequences in Belgium.
    Thiry, D. ; Mauroy, A. ; Saegerman, C. ; Thomas, I. ; Wautier, M. ; Miry, C. ; Czaplicki, G. ; Berkvens, D. ; Praet, N. ; Poel, W.H.M. van der; Cariolet, R. ; Brochier, B. ; Thiry, E. - \ 2014
    Veterinary Microbiology 172 (2014)3-4. - ISSN 0378-1135 - p. 407 - 414.
    antibodies - swine - transmission - infection - netherlands - populations - prevalence - france - meat - farm
    Zoonotic transmission of hepatitis E virus (HEV) is of special concern, particularly in high income countries were waterborne infections are less frequent than in developing countries. High HEV seroprevalences can be found in European pig populations. The aims of this study were to obtain prevalence data on HEV infection in swine in Belgium and to phylogenetically compare Belgian human HEV sequences with those obtained from swine. An ELISA screening prevalence of 73% (95% CI 68.8–77.5) was determined in Belgian pigs and a part of the results were re-evaluated by Western blot (WB). A receiver operating characteristic curve analysis was performed and scenarios varying the ELISA specificity relative to WB were analysed. The seroprevalences estimated by the different scenarios ranged between 69 and 81% and are in agreement with the high exposure of the European pig population to HEV. Pig HEV sequences were genetically compared to those detected in humans in Belgium and a predominance of genotype 3 subtype f was shown in both swine and humans. The high HEV seroprevalence in swine and the close phylogenetic relationships between pig and human HEV sequences further support the risk for zoonotic transmission of HEV between humans and pigs.
    Metal exposure and accumulation patterns in free-range cows (Bos taurus) in a contaminated natural area: Influence of spatial and social behavior
    Roggeman, S. ; Brink, N.W. van den; Praet, N. van; Blust, R. ; Bervoets, L. - \ 2013
    Environmental Pollution 172 (2013). - ISSN 0269-7491 - p. 186 - 199.
    hedgehog erinaceus-europaeus - potentially-toxic metals - dry-matter intake - heavy-metals - cadmium accumulation - trace-elements - environmental exposure - modeling cadmium - soil ingestion - mining area
    Possible effects of spatial metal distribution, seasonal-, ecological- and ethological parameters, on the metal exposure of cows were investigated. Therefore the habitat use, vegetation selection and foraging behavior of two free ranging Galloway herds in a metal polluted nature reserve were observed. Metal concentrations in soil, vegetation, hair, blood and feces were measured. Although both herds lived in the same reserve, their metal exposure differed significantly. A high consumption of soft rush by herd 1 during winter for instance was responsible for a large increase in daily Cd intake. The results of this study suggest that the exposure and health risks of large grazers can probably not only be predicted by general monitoring of soil and vegetation pollution. Also detailed information about the occurring vegetation types, spatial habitat use together with the social- and foraging behavior and diet selection of the species need to be studied.
    Development of a Decision Support System (DSS) to minimize the risk of contaminated areas to young children
    Praet, N. van; Baveco, J.M. ; Winter, W. de; Coen, W. De; Brink, N.W. van den; Bervoets, L. - \ 2009
    In: 19th SETAC Europe congress, Gothenburg, Sweden, 31 May - 4 June, 2009. - Gotherburg, Sweden : - p. 157 - 157.
    Differences in the indoor environment during pregnancy between ducth and immigrant women living in Rotterdam.
    Koopman, L.P. ; Jongste, J.C. de; Praet, I. ; Kerkhof, M. ; Gerritsen, J. ; Strien, R. van; Brunekreef, B. ; Wijga, A. ; Smit, H.A. ; Neijens, H.J. - \ 1998
    European Respiratory Journal 12 (1998). - ISSN 0903-1936 - p. 441S - 441S.
    Dietary and smoking habits of pregnant women with and without asthma or inhalant allergy in a birth cohort study.
    Wijga, A. ; Smit, H.A. ; Brunekreef, B. ; Kerkhof, M. ; Koopman, L.P. ; Praet, I. ; Strien, R. van - \ 1998
    European Respiratory Journal 12 (1998). - ISSN 0903-1936 - p. 210S - 210S.
    Response rates in the PIAMA-project: a study on the prevalence and incidence of asthma and mite allergy in the Netherlands.
    Strien, R. van; Brunekreef, B. ; Wijga, A. ; Smit, H.A. ; Koopman, L.P. ; Praet, I. ; Neijens, H.J. ; Jongste, J.C. de; Kerkhof, M. ; Gerritsen, J. - \ 1998
    European Respiratory Journal 12 (1998). - ISSN 0903-1936 - p. 210S - 210S.
    De preventie en incidentie van astma en mijtallergie (PIAMA-onderzoek): een prospectieve studie.
    Brunekreef, B. ; Aalberse, R.C. ; Gerritsen, J. ; Jongste, J.C. de; Kerkhof, M. ; Neijens, H.J. ; Praet, I. ; Rijcken, H.C.J. ; Smit, H.A. ; Strien, R. van; Wijga, A. - \ 1997
    Pulmonair 4 (1997). - ISSN 1380-6505 - p. 7 - 7.
    PIAMA: framework of a study on the Prevention and Incidence of Asthma and Mite Allergy.
    Strien, R.T. van; Praet, J. ; Kerkhof, M. ; Wijga, A. ; Jongste, J. de; Neijens, H. ; Gerritsen, J. ; Rijcken, B. ; Smit, H.A. ; Brunekreef, B. - \ 1997
    Journal of Allergy and Clinical Immunology 99 (1997). - ISSN 0091-6749 - p. S87 - S87.
    De preventie en incidentie van astma en mijtallergie (PIAMA-onderzoek): een prospectieve studie.
    Brunekreef, B. ; Aalberse, R.C. ; Gerritsen, J. ; Jongste, J.C. de; Kerkhof, M. ; Neijens, H.J. ; Praet, I. ; Rijcken, H.C.J. ; Smit, H.A. ; Strien, R. van; Wijga, A. - \ 1997
    Tijdschrift voor sociale geneeskunde 75 (1997)middenkatern. - ISSN 0040-7607 - p. 32 - 33.
    Controlling the ground reaction force during lifting
    Toussaint, H.M. ; Commissaris, D.A.C.M. ; Dieën, J.H. van; Reijnen, J.S. ; Praet, S.F.E. ; Beek, P.J. - \ 1995
    Journal of motor behavior 27 (1995). - ISSN 0022-2895 - p. 3 - 234.
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