Staff Publications

Staff Publications

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    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Variants of the recently discovered avian gyrovirus 2 are detected in Southern Brazil and The Netherlands
Santos, H.F. dos; Knak, M.B. ; Castro, F.L. de; Slongo, J. ; Ritterbusch, G.A. ; Klein, T.A.P. ; Esteves, P.A. ; Silva, A. Da; Trevisol, I.M. ; Claassen, E.A.W. ; Cornelissen, A.H.M. ; Lovato, M. ; Franco, A.C. ; Roehe, P.M. ; Rijsewijk, F.A.M. - \ 2012
Veterinary Microbiology 155 (2012)2-4. - ISSN 0378-1135 - p. 230 - 236.
chicken anemia virus - sequence alignment - disease virus - feathers - dna
A genome of a virus preliminarily named avian gyrovirus 2 (AGV2), a close relative to chicken anemia virus, was recently discovered in a chicken in the state of Rio Grande do Sul, Southern Brazil. To study the occurrence of AGV2 in Rio Grande do Sul and the neighboring state Santa Catarina, a number of adult chickens (n = 108 and n = 48, respectively) were tested for the presence of AGV2 DNA. An AGV2-specific PCR was developed, optimized and used to analyze DNA extracted from clinical samples. AGV2 DNA was detected in 98/108 (90.7%) of samples collected in the state of Rio Grande do Sul and 29/48 (60.4%) of the samples collected in the state of Santa Catarina. In order to check whether AGV2 DNA would be detected in samples from a geographically distant region, DNA from brain samples of 21 diseased chickens from the Netherlands were tested independently, by the same method. In such specimens, 9/21 (42.9%) brain tissue samples were found to contain AVG2 DNA. Sequence analysis of some of the PCR products demonstrated that the amplified AGV2 sequences could vary up to 15.8% and could preliminarily be divided in three groups. This indicated the occurrence of variants of AGV2, which may reflect differences in geographical origin and/or in biological properties. The data presented here provides evidence that AGV2 seems fairly distributed in chickens in Southern Brazil and that AGV2 also circulates in the Netherlands. Besides, circulating viruses display genetic variants whose significance should be further examined, particularly to determine whether AGV2 would play any role in chicken diseases.
BVDV virus-like particles
Schlapp, Tobias ; Rijsewijk, F. - \ 2011
Octrooinummer: EP2365082, verleend: 2011-09-14.
The present invention relates to bovine viral diarrhea virus (BVDV) virus-like particles, a polycistronic RNA and DNA corresponding thereto encoding a polyprotein of BVDV structural proteins that are sufficient to form BVDV virus-like particles, a viral vector encoding factors and structural proteins for the assembly of BVDV virus-like particles, a vaccine comprising BVDV virus-like particles, a diagnostic kit and methods for preparing BVDV virus-like particles.
Comparison of different prime-boost regimes with DNA and recombinant Orf virus based vaccines expressing glycoprotein D of pseudorabies virus in pigs
Rooij, E.M.A. van; Rijsewijk, F.A.M. ; Moonen-Leusen, H.W. ; Bianchi, A.T.J. ; Rziha, H.J. - \ 2010
Vaccine 28 (2010)7. - ISSN 0264-410X - p. 1808 - 1813.
aujeszkys-disease - cellular-immunity - vaccination - infection - antigen - protection - responses - antibody - system - memory
Both DNA and Orf virus (ORFV; Parapox virus) based vaccines have shown promise as alternatives for conventional vaccines in pigs against pseudorabies virus (PRV) infection causing Aujeszky's disease. In the present study we evaluated the efficacy of different prime-boost regimes in pigs in terms of immunogenicity and protection against challenge infection with PRV. The different prime-boost regimes consisted of the homologous prime-boost regimes (DNA followed by DNA or ORFV followed by ORFV) and the heterologous prime-boost regimes (DNA followed by ORFV and ORFV followed by DNA), all based on glycoprotein D (gD) of PRV. Moreover, we compared the efficacy of the different prime-boost regimes with the efficacy of a conventional modified live vaccine (MLV). The different prime-boost regimes resulted in different levels of immunity and protection against challenge infection. Most effective was the regime of priming with DNA vaccine followed by boosting with the ORFV based vaccine. This regime resulted in strong antibody responses, comparable to the antibody responses obtained after prime-boost vaccination with a conventional MLV vaccine. Also with regard to protection, the prime DNA-boost ORFV regime performed better than the other prime-boost regimes. This study demonstrates the potential of a heterologous prime-boost vaccination strategy against PRV based on a single antigen, and that in the natural host, the pig.
Eerste jonge gladde slangen in Brabant
Rijsewijk, A. van - \ 2010
Nature Today 2010 (2010)15-08.
IJskonijnen onder de amfibieën
Rijsewijk, A. van; Werkman, E. - \ 2010
Nature Today 2010 (2010)10-01.
Slapers en langslapers
Rijsewijk, A. van - \ 2010
Nature Today 2010 (2010)18-02.
Dansende salamanders nu te zien
Rijsewijk, A. van - \ 2010
Nature Today 2010 (2010)18-03.
Gladde slangen mogelijk verlaat door slecht weer
Rijsewijk, A. van - \ 2010
Nature Today 2010 (2010)24-06.
Jonge levendbarende hagedissen vroeg dit jaar
Rijsewijk, A. van - \ 2009
Nature Today 2009 (2009)09-07.
Bruine kikkers slachtoffers van vrieskou?
Rijsewijk, A. van; Hankman, T. - \ 2009
Nature Today 2009 (2009)21-02.
Varicellovirus UL49.5 proteins differentially affect the function of the transporter associated with antigen processing, TAP
Koppers-Lalic, D. ; Verweij, M.C. ; Lipinska, A.D. ; Wang, Y. ; Quinten, E. ; Reits, E.A. ; Koch, J. ; Loch, S. ; Rezende, M.M. ; Daus, F.J. ; Bienkowska-Szewczyk, K. ; Osterrieder, N. ; Mettenleiter, T.C. ; Heemskerk, M.H.M. ; Tampe, R. ; Neefjes, J.J. ; Chowdhury, S.I. ; Ressing, M.E. ; Rijsewijk, F.A.M. ; Wiertz, E.J.H.J. - \ 2008
PLoS Pathogens 4 (2008)5. - ISSN 1553-7366 - 14 p.
mhc class-i - major histocompatibility complex - peptide-loading complex - cytomegalovirus us6 glycoprotein - viral immune evasion - equine herpesvirus-1 - molecular-mechanism - down-regulation - finger protein - ligase mk3
Cytotoxic T-lymphocytes play an important role in the protection against viral infections, which they detect through the recognition of virus-derived peptides, presented in the context of MHC class I molecules at the surface of the infected cell. The transporter associated with antigen processing (TAP) plays an essential role in MHC class I-restricted antigen presentation, as TAP imports peptides into the ER, where peptide loading of MHC class I molecules takes place. In this study, the UL49.5 proteins of the varicelloviruses bovine herpesvirus 1 (BHV-1), pseudorabies virus (PRV), and equine herpesvirus 1 and 4 (EHV-1 and EHV-4) are characterized as members of a novel class of viral immune evasion proteins. These UL49.5 proteins interfere with MHC class I antigen presentation by blocking the supply of antigenic peptides through inhibition of TAP. BHV-1, PRV, and EHV- 1 recombinant viruses lacking UL49.5 no longer interfere with peptide transport. Combined with the observation that the individually expressed UL49.5 proteins block TAP as well, these data indicate that UL49.5 is the viral factor that is both necessary and sufficient to abolish TAP function during productive infection by these viruses. The mechanisms through which the UL49.5 proteins of BHV-1, PRV, EHV-1, and EHV-4 block TAP exhibit surprising diversity. BHV-1 UL49.5 targets TAP for proteasomal degradation, whereas EHV-1 and EHV-4 UL49.5 interfere with the binding of ATP to TAP. In contrast, TAP stability and ATP recruitment are not affected by PRV UL49.5, although it has the capacity to arrest the peptide transporter in a translocation-incompetent state, a property shared with the BHV-1 and EHV-1 UL49.5. Taken together, these results classify the UL49.5 gene products of BHV-1, PRV, EHV-1, and EHV-4 as members of a novel family of viral immune evasion proteins, inhibiting TAP through a variety of mechanisms.
Haemonchus contortus: Characterization of the baculovirus expressed form of aminopeptidase H11
Reszka, N. ; Rijsewijk, F.A.M. ; Zelnik, V. ; Moskwa, B. ; Bienkowska-Szewczyk, K. - \ 2007
Experimental Parasitology 117 (2007)2. - ISSN 0014-4894 - p. 208 - 213.
gut membrane-proteins - gastrointestinal nematode parasites - glutathione-s-transferase - anthelmintic resistance - schistosoma-mansoni - protective antigen - n-glycans - vaccination - vaccines - sheep
Recombinant form of Haemonchus contortus aminopeptidase H11, an intestinal membrane glycoprotein considered to be in its native form the most promising vaccine candidate, was produced in insect cells, characterised and tested in pilot vaccination-challenge trial on sheep. The sequence of the cloned gene, obtained by RT PCR isolated from adult worms, showed 97% identity to the highly immunogenic H11 clone, described by Graham et al., (database accession number AJ249941.1). A 1305 bp fragment of H11 was expressed in E. coli and used to raise a specific antiserum, which recognized recombinant forms of H11 and 110 kDa protein from H. contortus extract. H11 was expressed by baculovirus recombinants in insect cells in full length and as a fusion protein with H. contortus glutathione S-transferase (GST). The baculovirus produced recombinant antigens were used without adjuvants to immunize sheep, which resulted in 30% (full length H11) and 20% (GST-H11) reduction of worm burden. These animal experiments indicated that, although the protection induced by in vitro produced protein is lower than in case of H11 isolated from worms, recombinant forms of aminopeptidase may be considered as antigens for the control of haemonchosis.
Vaccination with a gE-negative bovine herpesvirus type 1 vaccine confers insufficient protection to a bovine herpesvirus type 5 challenge
Silva, A.D. ; Spilki, F.R. ; Franco, A.C. ; Esteves, P.A. ; Hubner, S.O. ; Driemeier, D. ; Oliveira, A.P. ; Rijsewijk, F.A.M. ; Roehe, P.M. - \ 2006
Vaccine 24 (2006)16. - ISSN 0264-410X - p. 3313 - 3320.
to-cell spread - glycoprotein-e - restriction-endonuclease - experimental-infection - neurological disease - nervous-system - 1.2a bhv-1.2a - in-vitro - virus - calves
In the present study, cross-protection to bovine herpesvirus type 5 (BHV-5) induced by bovine herpesvirus type 1 (BHV-1) vaccination was examined following inoculation of rabbits and calves with a glycoprotein E (gE)-negative BHV-1 vaccine and subsequent challenge with BHV-5. Rabbits (n = 5) and calves (n = 8) were vaccinated [five rabbits intranasally (IN), four calves IN and four intramuscularly (IM)] with 7.1 log10median tissue culture infective dose (TCID50) of the BHV-1 vaccine. Rabbits and calves were challenged IN [rabbits 2 weeks post-vaccination (pv); calves 5 weeks pv] with 9.1 log10 TCID50 of BHV-5. Two out of five vaccinated rabbits died after challenge with typical BHV-5 disease, as did 3/5 non-vaccinated controls. In calves, 4/8 vaccinated animals displayed mild signs of disease, whereas 6/6 non-vaccinated controls developed signs of disease, so severe that 2/6 had to be killed. Besides, nasal virus shedding post-challenge was not reduced by vaccination. At necropsy, on day 21 post-challenge, typical BHV-5 lesions were evident in brain tissues of both vaccinated and non-vaccinated calves. Dexametasone administration at 180 days post-infection did not reactivate clinical signs despite BHV-5 shedding in nasal secretions of both vaccinated and non-vaccinated calves. These results show that the BHV-1 vaccine evaluated here did not confer protection to BHV-5 in rabbits. In calves, BHV-1 vaccination did confer some protection to BHV-5 induced clinical disease, but it did not prevent infection and had no effect on nasal virus shedding or on the development of encephalitic lesions.
Bovine hemesvirus 1 UL49.5 protein inhibits the transporter associated with antigen processing despite complex formation with glycoprotein M
Lipinska, A.D. ; Koppers-Lalic, D. ; Rychlowski, M. ; Admiraal, P. ; Rijsewijk, F.A.M. ; Bienkowska-Szewczyk, K. ; Wiertz, E. - \ 2006
Journal of Virology 80 (2006)12. - ISSN 0022-538X - p. 5822 - 5832.
peptide-loading complex - disulfide-linked complex - class-i molecules - to-cell spread - bovine herpesvirus-1 - equine herpesvirus-1 - pseudorabies virus - finger protein - homolog gene - gm homolog
Bovine herpesvirus 1 (BHV-1) interferes with peptide translocation by the transporter associated with antigen processing (TAP). Recently, the UL49.5 gene product of BHV-1 was identified as the protein responsible for the observed inhibition of TAP. In BHV-1-infected cells and virions, the UL49.5 protein forms a complex with glycoprotein M (gM). Hence, it was investigated whether UL49.5 can combine the interactions with gM and the TAP complex. In cell lines constitutively expressing both UL49.5 and gM, UL49.5 appears to be required for functional processing of gM. Immunofluorescence-confocal laser scanning microscopy demonstrated that both proteins are interdependent for their redistribution from the endoplasmic reticulum to the trans-Golgi network. Remarkably, expression of cloned gM results in the abrogation of the UL49.5-mediated inhibition of TAP and prevents the degradation of the transporter. However, in BHV-1-infected cells, differences in UL49.5 and gM expression kinetics were seen to create a window of opportunity at the early stages of infection, during which time the UL49.5 protein can act on TAP without gM interference. Moreover, in later periods, non-gM-associated UL49.5 can be detected in addition to the UL49.5/gM complex. Thus, it has been deduced that different functions of UL49.5, editing of gM processing and inhibition of TAP, can be combined during BHV-1 infection.
On the role of feral ruminants in the transmission of bovine herpesvirus 1 to domestic cattle
Mollema, E. - \ 2006
Wageningen University. Promotor(en): Mart de Jong, co-promotor(en): F.A.M. Rijsewijk; Paul Koene. - - 135
runderherpesvirus 1 - rundvee - huisdieren - herkauwers - wilde kuddes - ziekteoverdracht - ziektebestrijding - persistentie - vaccinatie - ziektemodellen - epidemiologie - natuurreservaten - nederland - bovine herpesvirus 1 - cattle - domestic animals - ruminants - feral herds - disease transmission - disease control - persistence - vaccination - disease models - epidemiology - nature reserves - netherlands
Antigenic and molecular characterization of eight samples of Aujeszky's disease virus isolated in the state of Rio Grande do Sul, Brazil, in 2003
Silva, A.D. Da; Sortica, V.A. ; Braga, A.C. ; Spilki, F.R. ; Franco, A.C. ; Esteves, P.A. ; Rijsewijk, F.A.M. ; Rosa, J.C.A. ; Batista, H. ; Oliveira, A.P. ; Roehe, P.M. - \ 2005
Pesquisa Veterinária Brasileira 25 (2005)1. - ISSN 0100-736X - p. 21 - 24.
Pseudorabies or Aujeszky's disease (AD), caused by pseudorabies virus (PRV) is a major concern in swine production. In the state of Rio Grande do Sul, Brazil, AD was only detected in 1954, in cattle. In 2003 two outbreaks of encephalitis occurred on the northern region of the state, close to the border with the state of Santa Catarina. Pseudorabies virus (PRV) was isolated from distinct farms within the region and subjected to antigenic and genomic analyses. These isolates were compared with prototype strains NIA-3 and NP. Antigenic characterization with a panel of monoclonal antibodies (Mabs) directed to viral glycoproteins (gB, gC, gD and gE-,) was performed by an imunoperoxidase monolayer assay (IPMA) on infected cell monolayers. Genomic characterization was carried out by restriction enzyme analysis (REA) of the whole DNA viral genome with Bam HI. The antigenic profile of the eight isolates from Rio Grande do Sul as well as strains NIA-3 and NP were similar. REA analysis revealed that all isolates from Rio Grande do Sul displayed a genomic type II arrangement, a genotype often found in other outbreaks of AD previously reported in other Brazilian states. The results obtained suggest that the eight isolates examined here were similar.
Fasciola hepatica procathepsin L3 protein expressed by a baculovirus recombinant can partly protect rats against fasciolosis
Reszka, N. ; Cornelissen, J.B.W.J. ; Harmsen, M.M. ; Bree, J. de; Boersma, W.J.A. ; Rijsewijk, F.A.M. - \ 2005
Vaccine 23 (2005)23. - ISSN 0264-410X - p. 2987 - 2993.
cathepsin-l proteinases - saccharomyces-cerevisiae - functional expression - liver fluke - vaccines - sheep - identification - vaccination - ruminants - immunity
Fasciola hepatica juveniles express immunodominant cathepsin L proteins, which are mainly found in their immature, procathepsin form. A gene encoding such a procathepsin L (FheCL3) was expressed by a baculovirus recombinant and by Saccharomyces cerevisiae. The glycosylated FheCL3 proteins obtained by both systems were used in a vaccination/challenge experiment in rats. Both antigens evoked similar antibody responses, but only the baculovirus expressed FheCL3 caused a significant protection against the number of liver flukes (52% protection, P = 0.01), whereas the S. cerevisiae expressed FheCL3 did not. In a second experiment in rats, deglycosylated versions of both antigens were used, but this did not improve their efficacies.
Varicelloviruses avoid T cell recognition by UL49.5-mediated inactivation of the transporter associated with antigen processing
Koppers-Lalic, D. ; Reits, E.A. ; Ressing, M.E. ; Lipinska, A.D. ; Abele, R. ; Koch, J. ; Marcondes Rezende, M. ; Admiraal, P. ; Leeuwen, D. ; Bienkowska-Szewczyk, K. ; Mettenleiter, T.C. ; Rijsewijk, F.A.M. ; Tampe, R. ; Neefjes, J. ; Wiertz, E.J. - \ 2005
Proceedings of the National Academy of Sciences of the United States of America 102 (2005)14. - ISSN 0027-8424 - p. 5144 - 5149.
class-i expression - disulfide-linked complex - peptide-loading complex - virus protein icp47 - endoplasmic-reticulum - equine herpesvirus-1 - glycoprotein-m - transmembrane domains - molecular-mechanism - down-regulation
Detection and elimination of virus-infected cells by cytotoxic T lymphocytes depends on recognition of virus-derived peptides presented by MHC class I molecules. A critical step in this process is the translocation of peptides from the cytoplasm into the endoplasmic reticulum by the transporter associated with antigen processing (TAP). Here, we identified the bovine herpesvirus 1-encoded UL49.5 protein as a potent inhibitor of TAP. The expression of UL49.5 results in down-regulation of MHC class I molecules at the cell surface and inhibits detection and lysis of the cells by cytotoxic T lymphocytes. UL49.5 homologs encoded by two other varicelloviruses, pseudorabies-virus and equine herpesvirus 1, also block TAP. Homologs of UL49.5 are widely present in herpesviruses, acting as interaction partners for glycoprotein M, but in several varicelloviruses UL49.5 has uniquely evolved additional functions that mediate its participation in TAP inhibition. Inactivation of TAP by UL49.5 involves two events: inhibition of peptide transport through a conformational arrest of the transporter and degradation of TAP by proteasomes. UL49.5 is degraded along with TAP via a reaction that requires the cytoplasmic tail of UL49.5. Thus, UL49.5 represents a unique immune evasion protein that inactivates TAP through a unique two-tiered process
Studies on antigenic and genomic properties of Brazilian rabies virus isolates
Schaefer, R. ; Batista, H.B. ; Franco, A.C. ; Rijsewijk, F.A.M. ; Roehe, P.M. - \ 2005
Veterinary Microbiology 107 (2005)3-4. - ISSN 0378-1135 - p. 161 - 170.
monoclonal-antibodies - rt-pcr - lyssaviruses - bats - discrimination - variants - proteins
Despite the recognized stability of rabies virus, differences among isolates from different species have been found. This work was carried out with the aim to identify antigenic and genomic differences in Brazilian rabies virus isolates and to verify whether such alterations would bear any relationship with the different hosts for the virus in nature. For that, 79 Brazilian rabies viruses isolated from different host species and from distinct regions within Brazil were submitted to antigenic characterization with a panel of 11 monoclonal antibodies (Mabs) directed to lyssavirus antigens and to genomic analyses by the reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of the N gene followed by restriction endonuclease analysis (REA). In addition, the nucleotide sequences of part of the N gene (225 bp) of seven isolates, taken as representative of the majority of the viruses under study, were determined. The analyses with the Mabs and RT-PCR/REA allowed the identification of two major groups of variants, the first formed by most isolates of cattle and bats and the second formed by viruses of dog origin. Partial sequencing of the N gene confirmed the similarity among isolates from cattle origin and those of vampire bats. However, viruses from non-haematophagous bats exhibited consistent differences from those of vampire bat isolates. Such findings suggest that the variants have evolved fairly stable modifications, which are not altered after passage in a dead-end host of a distinct species. No association could be established between antigenic or genomic alterations and geographic distribution of the isolates, which suggests that evolution of the virus has been directed to adaptation to the host species
Experimental infection of calves with a gI, gE, US9 negative bovine herpesvirus type 5
Hubner, S.O. ; Oliveira, A.P. ; Franco, A.C. ; Rijsewijk, F.A.M. ; Roehe, P.M. - \ 2005
Comparative Immunology Microbiology and Infectious Diseases 28 (2005)3. - ISSN 0147-9571 - p. 187 - 196.
neurological disease - glycoprotein-e - virus - virulence - bhv-5 - immunogenicity - pathogenesis - reactivation - deletion - rabbits
In this work, a role for the genes encoding glycoproteins I (gI) and E (gE) and the US9 protein of bovine herpesvirus type 5 (BHV-5) in neuropathogenicity and reactivation of latent infections was examined. Calves infected intranasally with a gI/gE/US9 deleted recombinant shed up to 102.85 TCID50/ml infectious virus in nasal secretions. Calves infected with the wild type BHV-5 parental virus shed up to 105 TCID50/ml virus. No signs of disease were observed in calves infected with the recombinant virus, whereas those infected with wild type virus displayed respiratory and neurological signs. The recombinant was only able to reach the basal portions of the central nervous system. In contrast, wild type virus was found widespread within the brain. Reactivation with dexamethasone 60 days post-infection resulted in reactivation of wild type virus, whereas the recombinant virus could not be reactivated. These studies demonstrate that genes gI, gE and US9 of BHV-5 are important for its neuropathogenicity and its ability to reactive from latency
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