Root branching toward water involves posttranslational modification of transcription factor ARF7
Orosa-Puente, Beatriz ; Leftley, Nicola ; Wangenheim, Daniel von; Banda, Jason ; Srivastava, Anjil K. ; Hill, Kristine ; Truskina, Jekaterina ; Bhosale, Rahul ; Morris, Emily ; Srivastava, Moumita ; Kümpers, Britta ; Goh, Tatsuaki ; Fukaki, Hidehiro ; Vermeer, Joop E.M. ; Vernoux, Teva ; Dinneny, José R. ; French, Andrew P. ; Bishopp, Anthony ; Sadanandom, Ari ; Bennett, Malcolm J. - \ 2018
Science 362 (2018)6421. - ISSN 0036-8075 - p. 1407 - 1410.
Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water by using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells. This differential expression pattern is regulated by posttranslational modification of ARF7 with the small ubiquitin-like modifier (SUMO) protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA (indole-3-acetic acid) repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.
Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans
Haas, B.J. ; Kamoun, S. ; Zody, M.C. ; Jiang, R.H.Y. ; Handsaker, R.E. ; Cano, L.M. ; Grabherr, M. ; Kodira, C.D. ; Raffaele, S. ; Torto-Alalibo, T. ; Bozkurt, T.O. ; Ah-Fong, A.M.V. ; Alvarado, L. ; Anderson, V.L. ; Armstrong, M.R. ; Avrova, A. ; Baxter, L. ; Beynon, J. ; Boevink, P.C. ; Bollmann, S.R. ; Bos, J.I.B. ; Bulone, V. ; Cai, G. ; Cakir, C. ; Carrington, J.C. ; Chawner, M. ; Conti, L. ; Costanzo, S. ; Ewan, R. ; Fahlgren, N. ; Fischbach, M.A. ; Fugelstad, J. ; Gilroy, E.M. ; Gnerre, S. ; Green, P.J. ; Grenville-Briggs, L.J. ; Griffith, J. ; Grunwald, N.J. ; Horn, K. ; Horner, N.R. ; Hu, C.H. ; Huitema, E. ; Jeong, D.H. ; Jones, A.M.E. ; Jones, J.D.G. ; Jones, R.W. ; Karlsson, E.K. ; Kunjeti, S.G. ; Lamour, K. ; Liu, Z. ; Ma, L. ; Maclean, D. ; Chibucos, M.C. ; McDonald, H. ; McWalters, J. ; Meijer, H.J.G. ; Morgan, W. ; Morris, P.F. ; Munro, C.A. ; O'Neill, K. ; Ospina-Giraldo, M. ; Pinzon, A. ; Pritchard, L. ; Ramsahoye, B. ; Ren, Q. ; Restrepo, S. ; Roy, S. ; Sadanandom, A. ; Savidor, A. ; Schornack, S. ; Schwartz, D.C. ; Schumann, U.D. ; Schwessinger, B. ; Seyer, L. ; Sharpe, T. ; Silvar, C. ; Song, J. ; Studholme, D.J. ; Sykes, S. ; Thines, M. ; Vondervoort, P.J.I. van de; Phuntumart, V. ; Wawra, S. ; Weide, R. ; Win, J. ; Young, C. ; Zhou, S. ; Fry, W. ; Meyers, B.C. ; West, P. van; Ristaino, J. ; Govers, F. ; Birch, P.R.J. ; Whisson, S.C. ; Judelson, H.S. ; Nusbaum, C. - \ 2009
Nature 461 (2009). - ISSN 0028-0836 - p. 393 - 398.
effector proteins - rxlr effectors - cell-death - plant - avirulence - avr3a - resistance - infection - genes
Phytophthora infestans is the most destructive pathogen of potato and a model organism for the oomycetes, a distinct lineage of fungus-like eukaryotes that are related to organisms such as brown algae and diatoms. As the agent of the Irish potato famine in the mid-nineteenth century, P. infestans has had a tremendous effect on human history, resulting in famine and population displacement(1). To this day, it affects world agriculture by causing the most destructive disease of potato, the fourth largest food crop and a critical alternative to the major cereal crops for feeding the world's population(1). Current annual worldwide potato crop losses due to late blight are conservatively estimated at $6.7 billion(2). Management of this devastating pathogen is challenged by its remarkable speed of adaptation to control strategies such as genetically resistant cultivars(3,4). Here we report the sequence of the P. infestans genome, which at similar to 240 megabases (Mb) is by far the largest and most complex genome sequenced so far in the chromalveolates. Its expansion results from a proliferation of repetitive DNA accounting for similar to 74% of the genome. Comparison with two other Phytophthora genomes showed rapid turnover and extensive expansion of specific families of secreted disease effector proteins, including many genes that are induced during infection or are predicted to have activities that alter host physiology. These fast-evolving effector genes are localized to highly dynamic and expanded regions of the P. infestans genome. This probably plays a crucial part in the rapid adaptability of the pathogen to host plants and underpins its evolutionary potential.
|Disease resistance mechanisms triggered by extracellular elicitors
Jones, J.D.G. ; Rivas, S. ; Heese-Peck, A. ; Rowland, O. ; Rujon, A. ; Ludwig, A. ; Sadanandom, A. ; Gonzalez-Lamothe, R. ; Burg, H.A. van den; Fritz-Laylin, L. ; Navarro, L. ; Sjolander, K. ; Tor, M. ; Holub, E. ; Wit, P.J.G.M. de - \ 2004
In: Book of Abstracts Plant Genomics European Meetings, Lyon, France, 22-25 September 2004 - p. 75 - 75.