- Maria A. Prusicki (1)
- C. Bastiaan de Snoo (1)
- Martin Bayer (1)
- Jose Belt van de (1)
- Vanesa Calvo-Baltanás (1)
- Lena Carstens (1)
- Liudmila Chelysheva (1)
- Sara Christina Stolze (1)
- Nico Dissmeyer (1)
- Christian Fleck (1)
- Mathilde Grelon (1)
- Yuki Hamamura (1)
- Hirofumi Harashima (2)
- M.A. Hink (2)
- Shinichiro Komaki (1)
- M.A.C.J. Kwaaitaal (1)
- Emma M. Keizer (1)
- Katja Müller (2)
- Hirofumi Nakagami (1)
- Zsuzsanna Orban-Nemeth (1)
- Rik P. Rosmalen van (1)
- Pablo Parra-Nuñez (1)
- Gaëtan Pochon (1)
- Monica Pradillo (1)
- Peter Schlögelhofer (1)
- Arp Schnittger (3)
- A. Schnittger (1)
- M. Schor (1)
- Felix Seifert (1)
- Kostika Sofroni (1)
- Daniel Vezon (1)
- A.J.W.G. Visser (2)
- S.C. Vries de (2)
- Erik Wijnker (3)
- T.G. Wijnker (1)
- Chao Yang (1)
The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis
Yang, Chao ; Sofroni, Kostika ; Wijnker, Erik ; Hamamura, Yuki ; Carstens, Lena ; Harashima, Hirofumi ; Stolze, Sara Christina ; Vezon, Daniel ; Chelysheva, Liudmila ; Orban-Nemeth, Zsuzsanna ; Pochon, Gaëtan ; Nakagami, Hirofumi ; Schlögelhofer, Peter ; Grelon, Mathilde ; Schnittger, Arp - \ 2019
The EMBO Journal (2019). - ISSN 0261-4189
ASY1 - ASY3 - CDKA;1 - chromosome axis - PCH2
Meiosis is key to sexual reproduction and genetic diversity. Here, we show that the Arabidopsis cyclin-dependent kinase Cdk1/Cdk2 homolog CDKA;1 is an important regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1 (ASY1), the Arabidopsis homolog of Hop1 (homolog pairing 1), essential for synaptonemal complex formation, as a target of CDKA;1. The phosphorylation of ASY1 is required for its recruitment to the chromosome axis via ASYNAPTIC 3 (ASY3), the Arabidopsis reductional division 1 (Red1) homolog, counteracting the disassembly activity of the AAA+ ATPase PACHYTENE CHECKPOINT 2 (PCH2). Furthermore, we have identified the closure motif in ASY1, typical for HORMA domain proteins, and provide evidence that the phosphorylation of ASY1 regulates the putative self-polymerization of ASY1 along the chromosome axis. Hence, the phosphorylation of ASY1 by CDKA;1 appears to be a two-pronged mechanism to initiate chromosome axis formation in meiosis.
The Cdk1/Cdk2 homolog CDKA;1 controls the recombination landscape in Arabidopsis
Wijnker, Erik ; Harashima, Hirofumi ; Müller, Katja ; Parra-Nuñez, Pablo ; Bastiaan de Snoo, C. ; Belt, Jose van de; Dissmeyer, Nico ; Bayer, Martin ; Pradillo, Monica ; Schnittger, Arp - \ 2019
Proceedings of the National Academy of Sciences of the United States of America 116 (2019)25. - ISSN 0027-8424 - p. 12534 - 12539.
Class - Cross-over interference - Crossovers - Cyclin-dependent kinase - Meiosis - Meiotic recombination
Little is known how patterns of cross-over (CO) numbers and distribution during meiosis are established. Here, we reveal that cyclin-dependent kinase A;1 (CDKA;1), the homolog of human Cdk1 and Cdk2, is a major regulator of meiotic recombination in Arabidopsis. Arabidopsis plants with reduced CDKA;1 activity experienced a decrease of class I COs, especially lowering recombination rates in centromere-proximal regions. Interestingly, this reduction of type I CO did not affect CO assurance, a mechanism by which each chromosome receives at least one CO, resulting in all chromosomes exhibiting similar genetic lengths in weak loss-of-function cdka;1 mutants. Conversely, an increase of CDKA;1 activity resulted in elevated recombination frequencies. Thus, modulation of CDKA;1 kinase activity affects the number and placement of COs along the chromosome axis in a dose-dependent manner.
Live cell imaging of meiosis in Arabidopsis thaliana
Prusicki, Maria A. ; Keizer, Emma M. ; Rosmalen, Rik P. van; Komaki, Shinichiro ; Seifert, Felix ; Müller, Katja ; Wijnker, Erik ; Fleck, Christian ; Schnittger, Arp - \ 2019
eLife 8 (2019). - ISSN 2050-084X
A. thaliana - cell biology - cyclin - development - meiosis - phragmoplast - plant biology - reproduction - spindle
To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation. We find that the states of these parameters are not randomly associated and identify 11 cellular states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and quantitatively altered landmarks in tam, foremost the formation of previously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachment to chromosomes.
Control of plant meiosis using virus-induced gene silencing (VIGS)
Calvo-Baltanás, Vanesa - \ 2019
Wageningen University. Promotor(en): J.H.S.G.M. de Jong; B.J. Zwaan, co-promotor(en): T.G. Wijnker; A. Schnittger. - Wageningen : Wageningen University - ISBN 9789463435673 - 162
Control of the meiotic cell division program in plants
Wijnker, T.G. ; Schnittger, A. - \ 2013
Plant Reproduction 26 (2013)3. - ISSN 2194-7953 - p. 143 - 158.
anaphase-promoting complex/cyclosome - cyclin-dependent-kinases - genome-wide analysis - phd-finger protein - arabidopsis-thaliana - male meiosis - chromosome synapsis - fission yeast - molecular characterization - developmental defects
While the question of why organisms reproduce sexually is still a matter of controversy, it is clear that the foundation of sexual reproduction is the formation of gametes with half the genomic DNA content of a somatic cell. This reduction in genomic content is accomplished through meiosis that, in contrast to mitosis, comprises two subsequent chromosome segregation steps without an intervening S phase. In addition, meiosis generates new allele combinations through the compilation of new sets of homologous chromosomes and the reciprocal exchange of chromatid segments between homologues. Progression through meiosis relies on many of the same, or at least homologous, cell cycle regulators that act in mitosis, e.g., cyclin-dependent kinases and the anaphase-promoting complex/cyclosome. However, these mitotic control factors are often differentially regulated in meiosis. In addition, several meiosis-specific cell cycle genes have been identified. We here review the increasing knowledge on meiotic cell cycle control in plants. Interestingly, plants appear to have relaxed cell cycle checkpoints in meiosis in comparison with animals and yeast and many cell cycle mutants are viable. This makes plants powerful models to study meiotic progression and allows unique modifications to their meiotic program to develop new plant-breeding strategies
|Fluorescence fluctuation analysis of receptor kinase dimerization.
Hink, M.A. ; Vries, S.C. de; Visser, A.J.W.G. - \ 2011
In: Plant Kinases: Methods and Protocols, Methods in Molecular Biology / Dissmeyer, N., Schnittger, A., Springer Science+Business Media, LLC 2011 (vol. 779 ) - ISBN 9781617792632 - 295 p.
|Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta.
Kwaaitaal, M.A.C.J. ; Schor, M. ; Hink, M.A. ; Visser, A.J.W.G. ; Vries, S.C. de - \ 2011
In: Plant Kinases: Methods and Protocols, Methods in Molecular Biology. / Dissmeyer, N., Schnittger, A., Springer - ISBN 9781617792632 - 295 p.