Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Waar komt dat gif toch vandaan?
    Poelman, Marnix - \ 2020

    Mosselen en oesters Onlangs werd in weekdieren te hoge waarden tetrodotoxine – een natuurlijk gif – aangetroffen. Alleen grote doses zijn schadelijk.

    Highly pathogenic avian influenza subtype H5Nx clade 2.3.4.4 outbreaks in Dutch poultry farms, 2014–2018 : Clinical signs and mortality
    Schreuder, Janneke ; Manders, Thijs T.M. ; Elbers, Armin R.W. ; Spek, Arco N. van der; Bouwstra, Ruth J. ; Stegeman, Arjan ; Velkers, Francisca C. - \ 2020
    Transboundary and Emerging Diseases (2020). - ISSN 1865-1674
    H5N6 subtype - H5N8 subtype - influenza A virus - mortality - poultry - signs and symptoms

    In recent years, different subtypes of highly pathogenic avian influenza (HPAI) viruses caused outbreaks in several poultry types worldwide. Early detection of HPAI virus infection is crucial to reduce virus spread. Previously, the use of a mortality ratio threshold to expedite notification of suspicion in layer farms was proposed. The purpose of this study was to describe the clinical signs reported in the early stages of HPAI H5N8 and H5N6 outbreaks on chicken and Pekin duck farms between 2014 and 2018 in the Netherlands and compare them with the onset of an increased mortality ratio (MR). Data on daily mortality and clinical signs from nine egg-producing chicken farms and seven Pekin duck farms infected with HPAI H5N8 (2014 and 2016) and H5N6 (2017–2018) in the Netherlands were analysed. In 12 out of 15 outbreaks for which a MR was available, MR increase preceded or coincided with the first observation of clinical signs by the farmer. In one chicken and two Pekin duck outbreaks, clinical signs were observed prior to MR increase. On all farms, veterinarians observed clinical signs of general disease. Nervous or locomotor signs were reported in all Pekin duck outbreaks, but only in two chicken outbreaks. Other clinical signs were observed less frequently in both chickens and Pekin ducks. Compared to veterinarians, farmers observed and reported clinical signs, especially respiratory and gastrointestinal signs, less frequently. This case series suggests that a MR with a set threshold could be an objective parameter to detect HPAI infection on chicken and Pekin duck farms at an early stage. Observation of clinical signs may provide additional indication for farmers and veterinarians for notifying a clinical suspicion of HPAI infection. Further assessment and validation of a MR threshold in Pekin ducks are important as it could serve as an important tool in HPAI surveillance programs.

    Limited changes in the fecal microbiome composition of laying hens after oral inoculation with wild duck feces
    Schreuder, Janneke ; Velkers, Francisca C. ; Bouwstra, Ruth J. ; Beerens, N. ; Stegeman, J.A. ; Boer, Willem F. de; Elbers, A.R.W. ; Hooft, Pim van; Feberwee, Anneke ; Bossers, A. ; Jurburg, Stephanie D. - \ 2019
    Poultry Science 98 (2019)12. - ISSN 0032-5791 - p. 6542 - 6551.
    Interspecies transmission of fecal microbiota can serve as an indicator for (indirect) contact between domestic and wild animals to assess risks of pathogen transmission, e.g., avian influenza. Here, we investigated whether oral inoculation of laying hens with feces of wild ducks (mallards, Anas platyrhynchos) resulted in a hen fecal microbiome that was detectably altered on community parameters or relative abundances of individual genera. To distinguish between effects of the duck inoculum and effects of the inoculation procedure, we compared the fecal microbiomes of adult laying hens resulting from 3 treatments: inoculation with wild duck feces (duck), inoculation with chicken feces (auto), and a negative control group with no treatment. We collected cloacal swabs from 7 hens per treatment before (day 0), and 2 and 7 D after inoculation, and performed 16S rRNA amplicon sequencing. No distinguishable effect of inoculation with duck feces on microbiome community (alpha and beta diversity) was found compared to auto or control treatments. At the individual taxonomic level, the relative abundance of the genus Alistipes (phylum Bacteroidetes) was significantly higher in the inoculated treatments (auto and duck) compared to the control 2 D after inoculation. Seven days after inoculation, the relative abundance of Alistipes had increased in the control and no effect was found anymore across treatments. These effects might be explained by the perturbation of the hen's microbiome caused by the inoculation procedure itself, or by intrinsic temporal variation in the hen's microbiome. This experiment shows that a single inoculation of fecal microbiota from duck feces to laying hens did not cause a measurable alteration of the gut microbiome community. Furthermore, the temporary change in relative abundance for Alistipes could not be attributed to the duck feces inoculation. These outcomes suggest that the fecal microbiome of adult laying hens may not be a useful indicator for detection of single oral exposure to wild duck feces.

    Willen de wolven zich melden, alsjeblieft?
    Jansman, H.A.H. - \ 2019
    Hoe erg is het stikstofprobleem?
    Vries, W. de - \ 2019
    Valuing biomarker diagnostics for dementia care: enhancing the reflection of patients, their care-givers and members of the wider public
    Burg, Simone van der; Schreuder, Floris H.B.M. ; Klijn, Catharina J.M. ; Verbeek, Marcel M. - \ 2019
    Medicine, Health Care and Philosophy 22 (2019)3. - ISSN 1386-7423 - p. 439 - 451.
    Care - Cure - Dementia - Early diagnostics - Ethics - Stakeholder discussion

    What is the value of an early (presymptomatic) diagnosis of dementia in the absence of effective treatment? There has been a lively scholarly debate over this question, but until now (future) patients have not played a large role in it. Our study supplements biomedical research into innovative diagnostics with an exlporation of its meanings and values according to (future) patients. Based on seven focusgroups with (future) patients and their care-givers, we conclude that stakeholders evaluate early diagnostics with respect to whether and how they expect it to empower their capacity to (self-) care. They value it, for instance, with respect to whether it (a) explains experienced complaints, (b) allows to start a process of psychological acceptance and social adaptation to the expected degeneration, (c) contributes to dealing with anxieties (with respect to inheritable versions of dementia), (d) informs adequately about when to start preparing for the end of life, (d) informs the planning of a request for euthanasia, or (e) allows society to deal with a growing amount of dementia patients. Our study suggests that information about disease is considered ‘harmful’ or ‘premature’ when recipients feel unable to act on that information in their (self-) care. The results of this research offers input to further ethical research. It invites to adopt a care perspective in evaluation and to seek ways to prevent the ‘harm’ that such diagnostic methods can bring about.

    Highly sensitive integrated optical biosensing platform based on an asymmetric Mach-Zehnder interferometer and material-selective (bio)functionalization
    Knoben, W. ; Besselink, G. ; Roeven, E. ; Zuilhof, H. ; Schütz-Trilling, A. ; Meer, A. van der; Scheres, L. ; Leeuwis, H. ; Falke, F. ; Schreuder, F. ; Heideman, R. ; Vlekkert, H. van den - \ 2018
    In: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. - Chemical and Biological Microsystems Society (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ) - ISBN 9781510897571 - p. 982 - 985.
    Integrated optics - Optical detection - Surface modification

    An innovative integrated optical biosensing platform with an extremely high sensitivity is presented. The platform uses TriPleX (Si3N4/SiO2) waveguide technology in an asymmetric Mach-Zehnder Interferometer (aMZI) configuration, which is intrinsically more sensitive than other waveguide-based sensor technologies and Surface Plasmon Resonance (SPR). The performance of the sensor is enhanced by material-selective functionalization of Si3N4 vs. SiO2, allowing site-selective immobilization of the bioreceptor to the waveguide surface. The rest of the chip surface is modified with an antifouling layer to reduce non-specific adsorption. Analyte binding is thus confined to the waveguide surface, which results in improved sensitivity and limit of detection.

    BIOFOS: micro-ring resonator-based biophotonic system for food analysis. Nut mycotoxin detection
    Romero, A. ; Ninot, A. ; Hermoso, J.F. ; Zergioti, I. ; Kouloumentas, Ch. ; Avramopoulos, H. ; Leeuwis, H. ; Schreuder, E. ; Graf, S. ; Knapp, H. ; Barthelmebs, L. ; Noguer, T. ; Tsekenis, G. ; Scheres, L. ; Smulders, M. ; Zuilhof, H. ; Heesink, G. ; Reguillo, L. ; Risquez, A. - \ 2018
    In: Proceedings of the 7th International Symposium on Almonds and Pistachios. - International Society for Horticultural Science (Acta Horticulturae ) - ISBN 9789462612167 - p. 339 - 343.
    Analysis of aflatoxin B1 - Biosensor - Food safety

    BIOFOS aims to further develop and validate a reusable and high-added value Lab-on-Chip (LoC) based, micro-biophotonic sensor platform for in situ monitoring of food contaminants. The Lab-on-Chip was tested on milk (aflatoxin M1, antibiotics and lactose), olive oil (pesticides and metals), nuts (aflatoxin B1) and dehydrated fruits (ochratoxin A). BIOFOS combines the most promising concepts from the photonic, biological, nanochemical and fluidic parts of Lab-on-Chip systems, aiming to achieve low sensitivity and high specificity, excellent reliability and compactness. Current methodologies for detection of food contamination based on heavy analytical tools cannot guarantee a safe and stable food supply. The reasons are the complexity, the long time-to-result (2-3 days) and the cost of these tools, which limit the number of samples that can be practically analyzed at food processing and storage sites. Preliminary results for almonds spiked with aflatoxin B1 are presented. First results suggest that BIOFOS has sensitivity enough for AFB1, even that the concentration is at ppb level. More data are still required, and many analyses are ongoing in the laboratory. Results are not enough to conclude about any characteristics of the device performance, even then repeatability seems very good (variation less than 3% at 25 ppb) and recovery is acceptable (78.4%), while decision limit and detection capability are still uncertain.

    Functional intron-derived miRNAs and host-gene expression in plants
    Shapulatov, Umidjon ; Hoogdalem, M.D. van; Schreuder, M.E.L. ; Bouwmeester, H.J. ; Abdurakhmonov, Ibrokhim Y. ; Krol, A.R. van der - \ 2018
    Plant Methods 14 (2018). - ISSN 1746-4811
    Background: Recently, putative pre-miRNAs locations have been identified in the introns of plant genes, raising the question whether such genes can show a dual functionality by having both correct maturation of the host gene pre-mRNA and maturation of the miRNAs from the intron. Here, we demonstrated that such dual functionality is indeed possible, using as host gene the firefly luciferase gene with intron (ffgLUC), and different artificial intronic miRNAs (aimiRNA) placed within the intron of ffgLUC. Results: The miRNAs were based on the structure of the natural miR319a. Luciferase (LUC) activity in planta was used to evaluate a correct splicing of the ffgLUC mRNA. Different target sequences were inserted into the aimiRNA to monitor efficiency of silencing of different target mRNAs. After adjusting the insertion cloning strategy, the ffgLUCaimiR-319a gene showed dual functionality with correct splicing of ffgLUC and efficient silencing of TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1 transcription factor genes targeted in-trans by aimiR-319a or targeting the transgene ffLUC in-cis by an aimiR-LUC. Silencing of endogenous target genes by aimiRNA or amiRNA is efficient both in transient assays and stable transformants. A behave as strong phenotype the PHYTOCHROME B (PHYB) gene was also targeted by ffgLUCaimiR-PHYB. The lack of silencing of the PHYB target was most likely due to an insensitive target site within the PHYB mRNA which can potentially form a double stranded stem structure. Conclusion: The combination of an overexpression construct with an artificial intronic microRNA allows for a simultaneous dual function in plants. The concept therefore adds new options to engineering of plant traits that require multiple gene manipulations.
    Droogte: Hoe stoppen we de verzilting van Nederland?
    Blom-Zandstra, M. - \ 2018
    Boer zoekt toekomst
    Wiskerke, J.S.C. - \ 2018

    Met deze speciale uitzending van Spraakmakers komen we vanochtend vanaf de boerderij van Arend Heida in de Flevopolder. Aanleiding is een groot onderzoek van dagblad Trouw, waarin boeren aangeven dat ze graag meer rekening willen houden met natuur, milieu en ons landschap. Alleen, dat vergroenen is lang niet altijd makkelijk blijkt. In het eerste deel van deze uitzending praten we over de uitkomsten van het onderzoek van Trouw met Hans Marijnissen, Han Wiskerke en boeren Johan Leenders, Heleen Lansink, Arend Heida en Harry Schreuder

    BIOFOS: A micro-ring resonator-based biophotonic system for food analysis - Application to olive oil contaminants
    Romero, A. ; Ninot, A. ; Hermoso, J.F. ; Zergioti, I. ; Kouloumentas, C. ; Avramopoulos, H. ; Leeuwis, H. ; Schreuder, E. ; Graf, S. ; Knapp, H. ; Barthelmebs, L. ; Noguer, T. ; Tsekenis, G. ; Scheres, L. ; Smulders, M. ; Zuilhof, H. ; Heesink, G. ; Reguillo, L. - \ 2018
    In: Proceedings of the 8th International Olive Symposium. - International Society for Horticultural Science (Acta Horticulturae ) - ISBN 9789462611955 - p. 505 - 509.
    Analysis of pesticides and heavy metals - Copper - Oil quality - Organophosphates

    Current detection methods for contaminants in food use high-tech equipment sited in specialized laboratories. This makes online quality control along the food chain difficult. Official regulations limit pesticide and heavy-metal contamination, mainly for organic production, although their analysis is quite expensive, requires skilled personnel and is very difficult to apply to a large number of oil batches. The BIOFOS Project (ICT-FP7-GA.N.611528) aims to develop and validate a Lab-on-a-Chip (LoC) multianalyser, based on micro-biophotonic sensors, for in-situ food contaminant analysis. This device includes biosensors for milk analysis (aflatoxin M1, antibiotic and lactose), nuts (aflatoxin B1), dried fruits (ochratoxin A) and olive oil (organophosphates and metals). BIOFOS combines four high-tech platforms: (1) a photonic platform based on micro-ring resonators (MRR) for signal quantification, (2) a biological platform based on aptamers for analyte detection, (3) a nanochemical platform to immobilize aptamers onto the chip surface and (4) a microfluidic platform for sample pre-treatment and its loading into the biosensor. The project is organized in work packages in order to develop the technological platforms. End-user requirements are also considered, as well as existing fast analysis kits that will be compared against the device. Validation protocols and exploitation plans are also included. BIOFOS applications for olive oil analysis are discussed, in terms of accuracy, sensitivity, time-to-result and cost per sample.

    Improvements in fitness are not obligatory for exercise training-induced improvements in CV risk factors
    Hartman, Yvonne A.W. ; Hopman, Maria T.E. ; Schreuder, Tim H. ; Verheggen, Rebecca J.H.M. ; Scholten, Ralph R. ; Oudegeest-Sander, Madelijn H. ; Poelkens, Fleur ; Maiorana, Andrew J. ; Naylor, Louise H. ; Willems, Peter H. ; Tack, Cees J. ; Thijssen, Dick H.J. ; Green, Daniel J. - \ 2018
    Physiological Reports 6 (2018)4. - ISSN 2051-817X
    Cardiovascular diseases - exercise training - physical fitness - risk factors
    The purpose of this study was to assess whether changes in physical fitness relate to changes in cardiovascular risk factors following standardized, center-based and supervised exercise training programs in subjects with increased cardiovascular risk. We pooled data from exercise training studies of subjects with increased cardiovascular risk (n = 166) who underwent 8–52 weeks endurance training. We determined fitness (i.e., peak oxygen uptake) and traditional cardiovascular risk factors (body mass index, blood pressure, total cholesterol, high-density lipoprotein cholesterol), before and after training. We divided subjects into quartiles based on improvement in fitness, and examined whether these groups differed in terms of risk factors. Associations between changes in fitness and in cardiovascular risk factors were further tested using Pearson correlations. Significant heterogeneity was apparent in the improvement of fitness and individual risk factors, with nonresponder rates of 17% for fitness, 44% for body mass index, 33% for mean arterial pressure, 49% for total cholesterol, and 49% for high-density lipoprotein cholesterol. Neither the number, nor the magnitude, of change in cardiovascular risk factors differed significantly between quartiles of fitness change. Changes in fitness were not correlated with changes in cardiovascular risk factors (all P > 0.05). Our data suggest that significant heterogeneity exists in changes in peak oxygen uptake after training, while improvement in fitness did not relate to improvement in cardiovascular risk factors. In subjects with increased cardiovascular risk, improvements in fitness are not obligatory for training-induced improvements in cardiovascular risk factors.
    Scepsis over proef om stadslucht te zuiveren
    Molen, M.K. van der - \ 2017
    Er zat sinds 1957 nog nooit zo veel vis in de Noordzee
    Hintzen, N.T. - \ 2017
    De visserman wordt niet gehoord
    Steins, Nathalie - \ 2016

    Tot hun woede mogen vissers sinds begin dit jaar hun bijvangst niet langer overboord zetten. Het verplicht aan land brengen kost de sector 25 miljoen euro. En de effecten? „Dat weet niemand.”

    Low-phosphate induction of plastidal stromules is dependent on strigolactones but not on the canonical strigolactone signaling component MAX2
    Vismans, Gilles ; Meer, Tom van der; Langevoort, Olivier ; Schreuder, Marielle ; Bouwmeester, Harro ; Peisker, Helga ; Dörman, Peter ; Ketelaar, Tijs ; Krol, Sander van der - \ 2016
    Plant Physiology 172 (2016)4. - ISSN 0032-0889 - p. 2235 - 2244.

    Stromules are highly dynamic protrusions of the plastids in plants. Several factors, such as drought and light conditions, influence the stromule frequency (SF) in a positive or negative way. A relatively recently discovered class of plant hormones are the strigolactones; strigolactones inhibit branching of the shoots and promote beneficial interactions between roots and arbuscular mycorrhizal fungi. Here, we investigate the link between the formation of stromules and strigolactones. This research shows a strong link between strigolactones and the formation of stromules: SF correlates with strigolactone levels in the wild type and strigolactone mutants (max2-1 max3-9), and SF is stimulated by strigolactone GR24 and reduced by strigolactone inhibitor D2.

    Balance of RNA sequence requirement and NS3/NS3a expression of segment 10 of orbiviruses
    Feenstra, Femke ; Gennip, René G.P. Van; Schreuder, Myrte ; Rijn, Piet A. van - \ 2016
    Journal of General Virology 97 (2016)2. - ISSN 0022-1317 - p. 411 - 421.

    Orbiviruses are insect-transmitted, non-enveloped viruses with a ten-segmented dsRNA genome of which the bluetongue virus (BTV) is the prototype. Viral non-structural protein NS3/NS3a is encoded by genome segment 10 (Seg-10), and is involved in different virus release mechanisms. This protein induces specific release via membrane disruptions and budding in both insect and mammalian cells, but also the cytopathogenic release that is only seen in mammalian cells. NS3/NS3a is not essential for virus replication in vitro with BTV Seg-10 containing RNA elements essential for virus replication, even if protein is not expressed. Recently, new BTV serotypes with distinct NS3/NS3a sequence and cell tropism have been identified. Multiple studies have hinted at the importance of Seg-10 in orbivirus replication, but the exact prerequisites are still unknown. Here, more insight is obtained with regard to the needs for orbivirus Seg-10 and the balance between protein expression and RNA elements. Multiple silent mutations in the BTV NS3a ORF destabilized Seg-10, resulting in deletions and sequences originating from other viral segments being inserted, indicating strong selection at the level of RNA during replication in mammalian cells in vitro. The NS3a ORFs of other orbiviruses were successfully exchanged in BTV1 Seg-10, resulting in viable chimeric viruses. NS3/NS3a proteins in these chimeric viruses were generally functional in mammalian cells, but not in insect cells. NS3/NS3a of the novel BTV serotypes 25 and 26 affected virus release from Culicoides cells, which might be one of the reasons for their distinct cell tropism.

    EIP-AGRI Focus Group; Protein Crops: final report
    Schreuder, R. ; Visser, C.L.M. de - \ 2014
    EIP-AGRI - 49
    organische meststoffen - organisch bodemmateriaal - middellandse-zeegebied - innovaties - duurzaamheid (sustainability) - bodemvruchtbaarheid - peulvruchten - akkerbouw - kennisoverdracht - teeltsystemen - organic fertilizers - soil organic matter - mediterranean region - innovations - sustainability - soil fertility - grain legumes - arable farming - knowledge transfer - cropping systems
    This report is the result of the EIP-AGRI Focus Group (FG) on Soil organic matter (SOM) content in Mediterranean regions, which was launched under the European Innovation Partnership Agricultural Productivity and Sustainability (EIP-AGRI). The Focus Group brought together 19 experts with different backgrounds and experiences to make recommendations on transferable innovative solutions for the purpose of improving soil organic matter content in the Mediterranean region in a cost-effective way while securing soil functionality and soil fertility in the specific context of Mediterranean regions
    The EU's dependency on soya bean import for the animal feed industry and potential for EU produced alternatives
    Visser, C.L.M. de; Schreuder, R. ; Stoddard, F. - \ 2014
    OCL Oilseeds and Fats, Crops and Lipids 21 (2014)4. - ISSN 2257-6614 - 8 p.
    The European Innovation Partnership Agri has set up a consultation process involving 20 experts from 11 EU countries to discuss the potential of a substantial increase in protein crop production in the EU. The dependency of Europe on soya bean meal imports and the associated drivers are described and underline the need for change. The EIP Agri process resulted in the assessment of the present-day yield gap of protein crops using an approach based on the market values of the protein, starch and plant oil components. Oil-based protein crops seemed to be overall better positioned than starch based protein crops because the price of oil levels is higher than that of starch. Alfalfa was identified as being interesting for regions where drying cost are low. The process also resulted in the identification of opportunities and constraints to be encountered by the innovation process, combining the knowledge and physical infrastructure, market structure, co-operation and interaction and the influence of culturally determined values and beliefs. Therefore, the recommendation is to develop a comprehensive approach to meet the challenge of substantially increasing the EU’s protein crop production
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