Development of multi-use platforms at sea: Barriers to realising Blue Growth
Burg, S.W.K. van den; Schupp, Maximilian Felix ; Depellegrin, Daniel ; Barbanti, Andrea ; Kerr, Sandy - \ 2020
Ocean Engineering 217 (2020). - ISSN 0029-8018
Aquaculture - Barriers - Blue growth - Multi-use platforms - Offshore energy - Typology
The recent H2020 Blue Growth projects MARIBE and MUSES investigated the potential of a variety of different combinations of economic activities in co-location or integrated in multi-use platforms. Both projects identified barriers - including regulatory, financing, liability and insurance issues; environmental concerns; stakeholder perceptions; and lack of appropriate skills – that hamper the development of multi-use platforms. The H2020 MARIBE project concluded that further funding for multi-use demonstrations should be provided to increase investor confidence and bring multi-use through the so-called Valley of Death. The H2020 MUSES project concluded that multi-use needs to be proactively facilitated and incentivised through public regulatory bodies and respective support programmes. This paper combines and analyses results from both projects in order to identify key research gaps and actions required for the continued development of multi-use platforms, based on a structured critical review of available peer-reviewed literature on the topic as well as reports of both the MUSES and MARIBE projects. Research gaps and actions are analysed based on a multi-use platform typology to inform developers, policy makers, academia and investors for future development of multi-use at sea.
Erratum to: The sponge microbiome project
Moitinho-Silva, Lucas ; Nielsen, Shaun ; Amir, Amnon ; Gonzalez, Antonio ; Ackermann, Gail L. ; Cerrano, Carlo ; Astudillo-Garcia, Carmen ; Easson, Cole ; Sipkema, Detmer ; Liu, Fang ; Steinert, Georg ; Kotoulas, Giorgos ; McCormack, Grace P. ; Feng, Guofang ; Bell, James J. ; Vicente, Jan ; Björk, Johannes R. ; Montoya, Jose M. ; Olson, Julie B. ; Reveillaud, Julie ; Steindler, Laura ; Pineda, Mari Carmen ; Marra, Maria V. ; Ilan, Micha ; Taylor, Michael W. ; Polymenakou, Paraskevi ; Erwin, Patrick M. ; Schupp, Peter J. ; Simister, Rachel L. ; Knight, Rob ; Thacker, Robert W. ; Costa, Rodrigo ; Hill, Russell T. ; Lopez-Legentil, Susanna ; Dailianis, Thanos ; Ravasi, Timothy ; Hentschel, Ute ; Li, Zhiyong ; Webster, Nicole S. ; Thomas, Torsten - \ 2018
GigaScience 7 (2018)12. - ISSN 2047-217X
The sponge microbiome project
Moitinho-Silva, Lucas ; Nielsen, Shaun ; Amir, Amnon ; Gonzalez, Antonio ; Ackermann, Gail L. ; Cerrano, Carlo ; Astudillo-Garcia, Carmen ; Easson, Cole ; Sipkema, Detmer ; Liu, Fang ; Steinert, Georg ; Kotoulas, Giorgos ; McCormack, Grace P. ; Feng, Guofang ; Bell, James J. ; Vicente, Jan ; Björk, Johannes R. ; Montoya, Jose M. ; Olson, Julie B. ; Reveillaud, Julie ; Steindler, Laura ; Pineda, Mari Carmen ; Marra, Maria V. ; Ilan, Micha ; Taylor, Michael W. ; Polymenakou, Paraskevi ; Erwin, Patrick M. ; Schupp, Peter J. ; Simister, Rachel L. ; Knight, Rob ; Thacker, Robert W. ; Costa, Rodrigo ; Hill, Russell T. ; Lopez-Legentil, Susanna ; Dailianis, Thanos ; Ravasi, Timothy ; Hentschel, Ute ; Li, Zhiyong ; Webster, Nicole S. ; Thomas, Torsten - \ 2017
GigaScience 6 (2017)10. - ISSN 2047-217X
16S rRNA gene - Archaea - Bacteria - Marine sponges - Microbial diversity - Microbiome - Symbiosis
Marine sponges (phylum Porifera) are a diverse, phylogenetically deep-branching clade known for forming intimate partnerships with complex communities of microorganisms. To date, 16S rRNA gene sequencing studies have largely utilised different extraction and amplification methodologies to target the microbial communities of a limited number of sponge species, severely limiting comparative analyses of sponge microbial diversity and structure. Here, we provide an extensive and standardised dataset that will facilitate sponge microbiome comparisons across large spatial, temporal, and environmental scales. Samples from marine sponges (n = 3569 specimens), seawater (n = 370), marine sediments (n = 65) and other environments (n = 29) were collected from different locations across the globe. This dataset incorporates at least 268 different sponge species, including several yet unidentified taxa. The V4 region of the 16S rRNA gene was amplified and sequenced from extracted DNA using standardised procedures. Raw sequences (total of 1.1 billion sequences) were processed and clustered with (i) a standard protocol using QIIME closed-reference picking resulting in 39 543 operational taxonomic units (OTU) at 97% sequence identity, (ii) a de novo clustering using Mothur resulting in 518 246 OTUs, and (iii) a new high-resolution Deblur protocol resulting in 83 908 unique bacterial sequences. Abundance tables, representative sequences, taxonomic classifications, and metadata are provided. This dataset represents a comprehensive resource of sponge-associated microbial communities based on 16S rRNA gene sequences that can be used to address overarching hypotheses regarding host-associated prokaryotes, including host specificity, convergent evolution, environmental drivers of microbiome structure, and the sponge-associated rare biosphere.
BLAST results most abundant 97% OTUs
Steinert, G. ; Taylor, Michael W. ; Deines, Peter ; Simister, Rachel L. ; Voogd, Nicole J. De; Hoggard, Michael ; Schupp, Peter J. - \ 2017
Wageningen University & Research
16S rRNA - NCBI blast - greengenes
Top 30 OTUs, sequences & best BLAST hits (nr/nt)
Guam Sponge Microbial OTU abundance & Taxonomy (Greengenes)
Steinert, G. ; Taylor, Michael W. ; Deines, Peter ; Simister, Rachel L. ; Voogd, Nicole J. De; Hoggard, Michael ; Schupp, Peter J. - \ 2016
Wageningen University & Research
OTU table - 16S rRNA - greengenes - taxonomy - sponges
16S rRNA gene OTU (97%) Greengenes classification and abundance table of sponge associated microbial communities from Guam (USA) - 2247 OTUs
In four shallow and mesophotic tropical reef sponges from Guam the microbial community largely depends on host identity
Steinert, Georg ; Taylor, Michael W. ; Deines, Peter ; Simister, Rachel L. ; Voogd, Nicole J. De; Hoggard, Michael ; Schupp, Peter J. - \ 2016
PeerJ 2016 (2016)4. - ISSN 2167-8359
16S rRNA - Environmental variability - Microbial diversity - Porifera - Pyrosequencing - Symbiosis
Sponges (phylum Porifera) are important members of almost all aquatic ecosystems, and are renowned for hosting often dense and diverse microbial communities. While the specificity of the sponge microbiota seems to be closely related to host phylogeny, the environmental factors that could shape differences within local sponge-specific communities remain less understood. On tropical coral reefs, sponge habitats can span from shallow areas to deeper, mesophotic sites. These habitats differ in terms of environmental factors such as light, temperature, and food availability, as well as anthropogenic impact. In order to study the host specificity and potential influence of varying habitats on the sponge microbiota within a local area, four tropical reef sponges, Rhabdastrella globostellata, Callyspongia sp., Rhaphoxya sp., and Acanthella cavernosa, were collected from exposed shallow reef slopes and a deep reef drop-off. Based on 16S rRNA gene pyrosequencing profiles, beta diversity analyses revealed that each sponge species possessed a specific microbiota that was significantly different to those of the other species and exhibited attributes that are characteristic of high- and/or lowmicrobial- abundance sponges. These findings emphasize the influence of host identity on the associated microbiota. Dominant sponge- and seawater-associated bacterial phyla were Chloroflexi, Cyanobacteria, and Proteobacteria. Comparison of individual sponge taxa and seawater samples between shallow and deep reef sites revealed no significant variation in alpha diversity estimates, while differences in microbial beta diversity (variation in community composition) were significant for Callyspongia sp. sponges and seawater samples. Overall, the sponge-associated microbiota is significantly shaped by host identity across all samples, while the effect of habitat differentiation seems to be less predominant in tropical reef sponges.
|Seed allometry and disperser assemblages in tropical rain forests: a comparison of four floras on different continents
Forget, P.M. ; Dennis, A.J. ; Mazer, S.J. ; Jansen, P.A. ; Kitamura, S. ; Lambert, J.E. ; Westcott, D.A. - \ 2007
In: Seed dispersal: theory and its application in a changing world / Dennis, A.J., Green, R.J., Schupp, E.W., Westcott, D.A., Wallingford : CABI - ISBN 9781845931650 - p. 5 - 36.