Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Polysaccharides and phenolic compounds as substrate for yeasts isolated from rotten wood and description of Cryptococcus fagi sp.nov.
    Middelhoven, W.J. - \ 2006
    Antonie van Leeuwenhoek: : Nederlandsch tijdschrift voor hygiëne, microbiologie en serologie 90 (2006)1. - ISSN 0003-6072 - p. 57 - 67.
    anamorphic basidiomycetous yeast - rainy valdivian forest - phylogenetic-relationships - ascomycetous yeasts - genus trichosporon - aromatic-compounds - candida-shehatae - southern chile - decayed wood - identification
    Pieces of rotten wood collected in the forest were screened for the presence of yeasts. In spring time 3 tree species were sampled, followed by 9 species in summer. Yeast strains were identified by traditional methods. Identifications were confirmed by sequencing of ribosomal DNA in case of doubt. In total 14 yeast species of ascomycetous affiliation and 6 anamorphic basidiomycetous yeasts were isolated and identified. Most species were represented by only one strain, but Candida bertae by two and Trichosporon porosum by six strains, all from different wood samples. Three strains represented novel species, one of which is described as Cryptococcus fagi Middelhoven et Scorzetti. The type strain is CBS 9964 (JCM 13614). All strains were tested for growth on several polysaccharides as sole carbon source. Only some of these polymers supported growth of ascomycetous yeasts. Basidiomycetous yeasts assimilated soluble starch, pullulan, dextran, xylan, polygalacturonate, galactomannan and tannic acid or at least some of these. Cryptococcus podzolicus and T. porosum were the most active in this respect. None of the isolated strains grew on carboxymethyl cellulose, colloidal chitin, arabinogalactan and gum xanthan. Phenolic compounds were assimilated by several strains, belonging to the Trichosporonales and the Microbotryum and Stephanoascus/Blastobotrys clades, but not by members of the Tremellales (Cryptococcus musci excepted) and the Debaryomyces/Lodderomyces clade. Most of the ascomycetes assimilated n-hexadecane.
    Systematics of the anamorphic basidiomycetous yeast genus Trichosporon Behrend with the description of the five novel species: Trichosporon vadense, T. smithiae, T. dehoogii, T. scarabaeorum and T. gamsii
    Middelhoven, W.J. ; Scorzetti, G. ; Fell, J.W. - \ 2004
    International Journal of Systematic and Evolutionary Microbiology 54 (2004)3. - ISSN 1466-5026 - p. 975 - 986.
    sp nov. - aromatic-compounds - benzene compounds - sole source - uric-acid - identification - energy - carbon
    Phylogenetic trees of the anamorphic basichomycetous yeast genus Trichosporon Behrend, based on molecular sequence analysis of the internal transcribed spacer region and the D1/D2 region of the large subunit of ribosomal (26S) DNA, are presented. This study includes three novel species from soils, Trichosporon vadense sp. nov. (type strain, CBS 8901(T)), Trichosporon smithiae sp. nov. (type strain, CBS 8370(T)) and Trichosporon gamsii sp. nov. (type strain, CBS 8245(T)), one novel species from an insect, Trichosporon scarabaeorum sp. nov. (type strain, CBS 5601(T)) and one species of unknown origin, Trichosporon dehoogii sp. nov. (type strain, CBS 8686(T)). The phylogenetic positions and physiological characteristics that distinguish the new taxa from related species, based partly on growth tests that are not traditionally used in yeast taxonomy (uric acid, ethylamine, L-4-hydroxyproline, tyramine and L-phenylalanine as sources of carbon and nitrogen, and polygalacturonate, quinate, 4-ethylphenol, phloroglucinol, 2,3-dihydroxybenzoate and orcinol as sole carbon sources), are discussed. Assimilation of L-rhamnose and erythritol and maximum growth temperature were also used to delineate species.
    Trichosporon porosum comb. nov., an anarmorphoc basidiomycetous yeast inhabiting soil, related to the loubieri/laibachii group of species that assimilate hemicelluloses and phenolic compounds
    Middelhoven, W.J. ; Scorzetti, G. ; Fell, J.W. - \ 2001
    FEMS Yeast Research 1 (2001). - ISSN 1567-1356 - p. 15 - 22.
    Several isolates representing the genus Trichosporon were collected over a 6-year period from soils in The Netherlands. Based on classical growth tests with carbon and nitrogen compounds these were identical. Three of these (CBS 8396, CBS 8397 and CBS 8522) were subjected to molecular analysis of the D1/D2 region of the large subunit of rDNA. This confirmed that the three strains were identical, yet distinct from other members of the genus. Conspecificity was demonstrated with the type strain (CBS 2040) of Apiotrichum porosum Stautz (1931), with the exception that A. porosum, which had been isolated from exudate of a yew tree, differed morphologically from the soil strains. Based on the identity of DNA base sequences, morphology was not considered to be an adequate parameter to separate otherwise identical strains into two genera. Therefore, the new combination Trichosporon porosum is presented. Based on molecular sequence analysis, T. porosum may be related to T. sporotrichoides, within a weakly related clade that includes species such as Trichosporon laibachii and Trichosporon loubieri. The strains of T. porosum degrade phenolic compounds and hemicelluloses, which are characteristics with potential ecological importance in soil habitats. Characters distinguishing the nine species of the laibachii/loubieri group of species were listed. These include traditionally used tests as well as assimilation patterns of some aliphatic and phenolic compounds. Based on these tests, species such as Trichosporon multisporum and T. laibachii could be separated.
    Trichosporon veenhuisii sp. nov., an alkane-assimilating anamorphic basidiomycetous yeast
    Middelhoven, W.J. ; Scorzetti, G. ; Fell, J.W. - \ 2000
    International Journal of Systematic and Evolutionary Microbiology 50 (2000). - ISSN 1466-5026 - p. 381 - 387.
    A morphological and physiological description of an alkane-assimilating anamorphic basidiomycetous yeast species, named Trichosporon veenhuisii, is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nuclear base sequencing of the D1/D2 region of the large subunit of rDNA, is discussed. The type strain is CBS 7136(T).
    Trichosporon guehoae sp.nov., an anamorphic basidiomycetous yeast
    Middelhoven, W.J. ; Scorzetti, G. ; Fell, J.W. - \ 1999
    Yeast 68 (1999). - ISSN 0749-503X - p. 45 - 46.
    Trichosporon guehoae sp.nov., an anamorphic basidiomycetous yeast
    Middelhoven, W.J. ; Scorzetti, G. ; Fell, J.W. - \ 1999
    Canadian Journal of Microbiology 45 (1999)8. - ISSN 0008-4166 - p. 686 - 690.
    A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521(T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nuclear base sequencing of the D1/D2 region of the large subunit of rDNA is discussed.
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