- M. Broertjes (1)
- R. Debets (1)
- Ágnes Dergez (1)
- C.J.A.M. Doodeman (2)
- C.C.F.M. Govers (1)
- René H. Wijffels (1)
- Z. Ilovai (1)
- Marcel Janssen (1)
- Péter Kesseru (1)
- István Kiss (1)
- Ákos Koós (1)
- J.C. Lenteren van (3)
- Alfredo Martin (1)
- Ana Martins Pito de Magalhaes (1)
- K. Panoutsopoulou (1)
- C.J.A.M. Posthuma-Doodeman (1)
- Arjen Rinzema (1)
- J. Roszik (1)
- I. Sebestyen (3)
- Z. Sebestyen (1)
- Petra Sebestyén (2)
- J. Szollosi (1)
- A. Szoor (1)
- Gábor Tóth (1)
- T. Van (1)
- G. Vereb (1)
Microalgal biofilm growth under day-night cycles
Blanken, Ward ; Martins Pito de Magalhaes, Ana ; Sebestyén, Petra ; Rinzema, Arjen ; Wijffels, René H. ; Janssen, Marcel - \ 2017
Algal Research 21 (2017). - ISSN 2211-9264 - p. 16 - 26.
Carbon-partitioning - Day-night cycles - Microalgal biofilms - Modelling growth - Respiration
Microalgal biofilms of Chlorella sorokiniana were cultivated under simulated day-night cycles at high productivity and high photosynthetic efficiency. Comparing day-night to continuous illumination did not demonstrate differences in the light utilization efficiency. This indicates that biomass consumed overnight represents sugar consumption for synthesis of new functional biomass and maintenance related respiration. Modelling microalgal biofilm growth was employed to calculate maximum productivities and photosynthetic efficiencies. A light limited microalgal biofilm growth model in which both diurnal carbon-partitioning and maintenance under prolonged dark conditions were taken into account was developed, calibrated, and validated experimentally. Extended periods of darkness resulted in reduced maintenance related respiration. Based on simulations with the validated biofilm growth model, it could be determined that the photosynthetic efficiency of biofilm growth can be higher than that of suspension growth. This is related to the fact that the maintenance rate in the dark zones of the biofilm is lower compared to that in the dark zones of suspension cultures which are continuously mixed with the photic zone.
Upscale of a laboratory rotating disk biofilm reactor and evaluation of its performance over a half-year operation period in outdoor conditions
Sebestyén, Petra ; Blanken, Ward ; Bacsa, István ; Tóth, Gábor ; Martin, Alfredo ; Bhaiji, Tasneem ; Dergez, Ágnes ; Kesseru, Péter ; Koós, Ákos ; Kiss, István - \ 2016
Algal Research 18 (2016). - ISSN 2211-9264 - p. 266 - 272.
Attached cultivation - Flue gas - Microalga - Pilot scale - Sunlight
Biofilm-based microalgae cultivation techniques are promising technologies to overcome several issues of suspended cultivations, although only a few large-scale systems have been examined so far. In this study, a rotating biological contactor-based laboratory-scale Algadisk reactor of 0.39 m2 was tested under low light intensity, and then scaled up to 15.9 m2 and operated for 6 months in outdoor conditions in order to test its stability and biomass production efficiency with Chlorella sorokiniana. The highest biomass productivity observed in the lab-scale reactor on disk surface base was 3.2 g (m2 day)-1 with a 0.9 g mol-1 biomass yield on light and 208 g kg-1 dry weight content in biofilm. Due to pH crashes, extreme temperature variations, CO2 limitation, and failure of disk rotation, the Algadisk pilot system showed varying biomass productivity from 0.5 to 8.4 g (m2 day)-1on reactor footprint area. Also, biomass yield on light and biomass density remained lower than at laboratory scale. Nonetheless, a total of 7.4 kg CO2 was fixed in the biofilm during the operating time. Despite the difficulties and the complexity of the system, over 20 weeks of continuous operation was achieved without the need of reinoculation.
TCR's genetically linked to CD28 and CD3e do not mispair with endogous TCR chains and mediate enhanced T cell persistance and anti-melanoma activity
Govers, C.C.F.M. ; Sebestyen, Z. ; Roszik, J. ; Brakel, M. van; Berrevoets, C. ; Szoor, A. ; Panoutsopoulou, K. ; Broertjes, M. ; Van, T. ; Vereb, G. ; Szollosi, J. ; Debets, R. - \ 2014
The Journal of Immunology 193 (2014)10. - ISSN 0022-1767 - p. 5315 - 5326.
chimeric-antigen-receptor - gene-transfer - metastatic melanoma - cancer regression - lymphocytes - therapy - alpha - activation - toxicity - survival
Adoptive transfer of T cells that are gene engineered to express a defined TCR represents a feasible and promising therapy for patients with tumors. However, TCR gene therapy is hindered by the transient presence and effectiveness of transferred T cells, which are anticipated to be improved by adequate T cell costimulation. In this article, we report the identification and characterization of a novel two-chain TCR linked to CD28 and CD3e (i.e., TCR:28e). This modified TCR demonstrates enhanced binding of peptide–MHC and mediates enhanced T cell function following stimulation with peptide compared with wild-type TCR. Surface expression of TCR:28e depends on the transmembrane domain of CD28, whereas T cell functions depend on the intracellular domains of both CD28 and CD3e, with IL-2 production showing dependency on CD28:LCK binding. TCR:28e, but not wild-type TCR, induces detectable immune synapses in primary human T cells, and such immune synapses show significantly enhanced accumulation of TCR transgenes and markers of early TCR signaling, such as phosphorylated LCK and ERK. Importantly, TCR:28e does not show signs of off-target recognition, as evidenced by lack of TCR mispairing, as well as preserved specificity. Notably, when testing TCR:28e in immune-competent mice, we observed a drastic increase in T cell survival, which was accompanied by regression of large melanomas with limited recurrence. Our data argue that TCR transgenes that contain CD28, and, thereby, may provide T cell costimulation in an immune-suppressive environment, represent candidate receptors to treat patients with tumors.
|Short-range flight test for quality control of Encarsia formosa.
Posthuma-Doodeman, C.J.A.M. ; Lenteren, J.C. van; Sebestyen, I. ; Ilovai, Z. - \ 1996
Proceedings of the Section Experimental and Applied Entomology of the Netherlands Entomological Society 7 (1996). - ISSN 1388-8390 - p. 153 - 158.
|Tests of the flight to check the quality of Encarsia formosa populations.
Sebestyen, I. ; Doodeman, C.J.A.M. ; Lenteren, J.C. van - \ 1995
In: Proc. 41st Plant Protection Days. Budapest - p. 65 - 65.
|Short-range flight test for quality control of Encarsia formosa.
Doodeman, C.J.A.M. ; Sebestyen, I. ; Lenteren, J.C. van - \ 1994
Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent 59 (1994). - p. 315 - 323.