Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    ABC transporters from Aspergillus nidulans are involved in protection against cytotoxic agents and antibiotic production
    Andrade, A.C. ; Nistelrooy, J.G.M. van; Peery, R.B. ; Skatrud, P.L. ; Waard, M.A. de - \ 2000
    Molecular and General Genetics 263 (2000). - ISSN 0026-8925 - p. 966 - 977.
    This paper describes the characterization of atrC and atrD (ABC transporters C and D), two novel ABC transporter-encoding genes from the filamentous fungus Aspergillus nidulans, and provides evidence for the involvement of atrD in multidrug transport and antibiotic production. BLAST analysis of the deduced amino acid sequences of AtrCp and AtrDp reveals high homology to ABC transporter proteins of the P-glycoprotein cluster. AtrDp shows a particularly high degree of identity to the amino acid sequence of AfuMdr1p, a previously characterized ABC transporter from the human pathogen A. fumigatus. Northern analysis demonstrates an increase in transcript levels of atrC and atrD in fungal germlings upon treatment with natural toxic compounds and xenobiotics. The atrC gene has a high constitutive level of expression relative to atrD, which suggests its involvement in a metabolic function. Single knock-out mutants for atrC and atrD were generated by gene replacement using pyrG from A. oryzae as a selectable marker. &Dgr;atrD mutants display a hypersensitive phenotype to compounds such as cycloheximide, the cyclosporin derivative PSC 833, nigericin and valinomycin, indicating that AtrDp is involved in protection against cytotoxic compounds. Energy-dependent efflux of the azole-related fungicide fenarimol is inhibited by substrates of AtrDp (e.g. PSC 833, nigericin and valinomycin), suggesting that AtrDp plays a role in efflux of this fungicide. Most interestingly, &Dgr;atrD mutants display a decrease in penicillin production, measured indirectly as antimicrobial activity against Micrococcus luteus. These results suggest that ABC transporters may be involved in secretion of penicillin from fungal cells.
    ABC transporters in Aspergillus nidulans
    Andrade, A.C. ; Nistelrooy, J.G.M. van; Sorbo, G. Del; Skatrud, P.L. ; Waard, M.A. de - \ 1999
    In: Abstract Book 2nd FEBS Advanced lecture course 'ATP-binding cassette transporters : from multidrug resistance to genetic disease', Gosau, Austria, 20-27 February 1999. - [S.l.] : [s.n.], 1999 - p. 70 - 70.
    Characterization of ABC transporters from Aspergillus nidulans
    Andrade, A.C. ; Nistelrooy, J.G.M. van; Sorbo, G. Del; Skatrud, P.L. ; Waard, M.A. de - \ 1999
    Fungal Genetics Newsletter 46 (1999)supplement. - ISSN 0895-1942 - p. 95 - 95.
    The role of ABC transporters in Aspergillus nidulans
    Andrade, A. ; Nistelrooy, J. van; Sorbo, G. Del; Skatrud, P. ; Waard, M. de - \ 1999
    In: Book of abstracts : 9th International Congress on Molecular Plant-Microbe Interactions, Amsterdam, the Netherlands, 25-30 July 1999. - [S.l.] : [s.n.], 1999 - p. 78 - 78.
    ABC Transporters in Aspergillus nidulans.
    Andrade, A.C. ; Xuei, X. ; Sorbo, G. Del; Skatrud, P.L. ; Waard, M.A. de - \ 1998
    In: 7th International Congress of Plant Pathology, Edinburgh, UK (1998) 5.5.13
    ABC transporters in Aspergillus nidulans.
    Andrade, A.C. ; Xuei, X. ; Sorbo, G. Del; Skatrud, P.L. ; Waard, M.A. de - \ 1998
    In: 4th European Conference on Fungal Genetics, Leon, Spain - p. 146 - 146.
    Multiple drug resistance gene atrD of Aspergillus nidulans (X-11766)
    Skatrud, P. ; Waard, M.A. de; Andrade, A.C. - \ 1997
    Octrooinummer: WO9932608, gepubliceerd: 1997-12-23.
    The invention provides isolated nucleic acid compounds encoding a multiple drug resistance protein of Aspergillus nidulans. Vectors and transformed host cells comprising the multiple drug resistance-encoding DNA of Aspergillus nidulans atrD are also provided. The invention further provides assays which utilize these transformed host cells
    Multiple drug resistance gene atrC of Aspergillus nidulans (X-11765)
    Skatrud, P. ; Waard, M.A. de; Andrade, A.C. - \ 1997
    Octrooinummer: WO9932505, gepubliceerd: 1997-12-23.
    The invention provides isolated nucleic acid compounds encoding a multiple drug resistance protein of Aspergillus nidulans. Vectors and transformed host cells comprising the multiple drug resistance-encoding DNA of Aspergillus nidulans atrC are also provided. The invention further provides assays which utilize these transformed host cells
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