Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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    Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses
    Meide, N.M.A. van der; Schurink, A. ; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Schaap, P.J. ; Savelkoul, H.F.J. ; Tijhaar, E.J. - \ 2014
    Insect Bite Hypersensitivity (IBH) is a common IgE mediated skin allergy in horses, caused by the bites of Culicoides species (midges) that seriously affects the welfare of these horses. Currently no effective treatment and no reliable diagnostic test is available for this allergic condition. We identified midges of the C. obsoletus complex as the dominant Culicoides species feeding on horses in the Netherlands. Its transcriptome was determined and sequence similarity searches with allergens previously described forother midges (C. sonorensis and C. nubeculosus) revealed 7 potential allergens of C. obsoletus , named Cul o 1 – Cul o 7.The sequences encoding these allergens were cloned and expressed as his6-tagged recombinant proteins in E. coli andpurified on Ni2+ sepharose beads. The purified recombinant proteins were able to induce an allergic reaction after injection into the skin of IBH affected horses, but not in healthy control horses. They all showed significant (p
    Bluetongue, Schmallenberg - what is next? Culicoides-borne viral diseases in the 21st Century
    Koenraadt, C.J.M. ; Balenghien, T. ; Carpenter, S. ; Ducheyne, E. ; Elbers, A.R.W. ; Fife, M. ; Garros, C. ; Ibanez-Justicia, A. ; Kampen, H. ; Kormelink, R.J.M. ; Losson, B. ; Poel, W.H.M. van der; Regge, N. de; Rijn, P.A. van; Sanders, C. ; Schaffner, F. ; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Takken, W. ; Werner, D. ; Seelig, F. - \ 2014
    BMC Veterinary Research 10 (2014). - ISSN 1746-6148 - 6 p.
    flight mass-spectrometry - biting midges - diptera-ceratopogonidae - variipennis diptera - virus-infection - europe - vector - spp. - transmission - netherlands
    In the past decade, two pathogens transmitted by Culicoides biting midges (Diptera: Ceratopogonidae), bluetongue virus and Schmallenberg virus, have caused serious economic losses to the European livestock industry, most notably affecting sheep and cattle. These outbreaks of arboviral disease have highlighted large knowledge gaps on the biology and ecology of indigenous Culicoides species. With these research gaps in mind, and as a means of assessing what potential disease outbreaks to expect in the future, an international workshop was held in May 2013 at Wageningen University, The Netherlands. It brought together research groups from Belgium, France, Germany, Spain, Switzerland, United Kingdom and The Netherlands, with diverse backgrounds in vector ecology, epidemiology, entomology, virology, animal health, modelling, and genetics. Here, we report on the key findings of this workshop.
    Schmallenberg virus detected by RT-PCR in Culicoides biting midges captured during the 2011 epidemic in the Netherlands
    Elbers, A.R.W. ; Meiswinkel, R. ; Weezep, E. van; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Kooi, E.A. - \ 2013
    Schmallenberg virus detected by RT-PCR in Culicoides biting midges captured during the 2011 epidemic in the Netherlands
    Elbers, A.R.W. ; Meiswinkel, R. ; Weezep, E. van; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Kooi, E.A. - \ 2013
    Schmallenberg virus detected in Culicoides biting midges in the Netherlands in 2011
    Elbers, A.R.W. ; Meiswinkel, R. ; Weezep, E. van; Sloet van Oldruitenborgh-Ossterbaan, M.M. ; Kooi, E.A. - \ 2013
    In: Proceedings 16th International Symposium of the world Association of Veterinary Laboratory Diagnosticians, 5-8 June 2013, Berlin, Germay. - - p. 84 - 84.
    Schmallenberg virus detected in Culicoides biting midges in the Netherlands in 2011
    Elbers, A.R.W. ; Meiswinkel, R. ; Weezep, E. van; Sloet van Oldruitenborgh-Ossterbaan, M.M. ; Kooi, E.A. - \ 2013
    In: Proceedings International meeting on Emerging Diseases and Survreillance, Vienna, Austria, 15-18 February, 2013. - - p. 176 - 176.
    Cloning and expression of candidate allergens from Culicoides obsoletus for diagnosis of insect bite hypersensitivity in horses
    Meide, N.M.A. van der; Roders, N. ; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Schaap, P.J. ; Oers, M.M. van; Leibold, W. ; Savelkoul, H.F.J. ; Tijhaar, E. - \ 2013
    Veterinary Immunology and Immunopathology 153 (2013)3-4. - ISSN 0165-2427 - p. 227 - 239.
    lepidoglyphus-destructor - intradermal challenge - escherichia-coli - skin - identification - extract - ige - immunotherapy - antibodies - responses
    Insect bite hypersensitivity (IBH) is an IgE-mediated (Type I) hypersensitivity reaction induced by allergens from biting midges of the Culicoides spp. The aim of the present study was to identify, clone and express recombinant allergens from C. obsoletus, the main species found feeding on horses in the Netherlands, by sequence homology searches on the C. obsoletus specific RNA database, with previously described allergens from C. nubeculosus and C. sonorensis. BLAST searches with these described allergens resulted in similarity hits with 7 genes coding for C. obsoletus allergens. These allergens were expressed as hexahistidine tagged recombinant proteins in E. coli. Allergens were termed Cul o 1–Cul o 7. A maltase (Cul o 1) plus Cul s 1 (maltase of C. sonorensis) were additionally expressed in insect cells using the baculovirus expression system to compare homologous allergens from different species produced with different expression systems in diagnostic in vitro and in vivo tests. We demonstrate that IBH affected horses in the Netherlands show higher IgE levels to Cul o 1 than to Cul s 1, as determined by an IgE ELISA. Furthermore, we show that Cul o 1 produced in E. coli is at least as suitable for in vitro diagnosis of IBH affected horses as Cul o 1 produced in the baculovirus/insect cell expression system. The resulting proteins were evaluated for their ability to discriminate IBH affected and healthy horses by ELISA and intradermal testing. The frequency of positive test results by ELISA within IBH affected horses ranged from 38% to 67% for the different allergens. When results of IgE-binding to Cul o 1–Cul o 7 were combined the test had a sensitivity of 92% and specificity of 85%. The capability of the allergens to induce Type I hypersensitivity reaction in IBH affected horses was demonstrated by an intradermal test. The results show that E. coli expressed recombinant allergens from C. obsoletus are valuable tools to determine the allergen specific sensitisation profile (component resolved diagnosis) in horses with IBH in countries were C. obsoletus is the most abundant species and may facilitate in the development of future immunotherapy
    Physiological and behavioral responses of horses during police training
    Munsters, C.C.B.M. ; Visser, E.K. ; Broek, J. van den; Sloet van Oldruitenborgh-Oosterbaan, M.M. - \ 2013
    Animal 7 (2013)5. - ISSN 1751-7311 - p. 822 - 827.
    heart-rate-variability - dogs
    Mounted police horses have to cope with challenging, unpredictable situations when on duty and it is essential to gain insight into how these horses handle stress to warrant their welfare. The aim of the study was to evaluate physiological and behavioral responses of 12 (six experienced and six inexperienced) police horses during police training. Horses were evaluated during four test settings at three time points over a 7-week period: outdoor track test, street track test, indoor arena test and smoke machine test. Heart rate (HR; beats/min), HR variability (HRV; root means square of successive differences; ms), behavior score (BS; scores 0 to 5) and standard police performance score (PPS; scores 1 to 0) were obtained per test. All data were statistically evaluated using a linear mixed model (Akaike's Information criterium; t > 2.00) or logistic regression (P <0.05). HR of horses was increased at indoor arena test (98 ± 26) and smoke machine test (107 ± 25) compared with outdoor track (80 ± 12, t = 2.83 and t = 3.91, respectively) and street track tests (81 ± 14, t = 2.48 and t = 3.52, respectively). HRV of horses at the indoor arena test (42.4 ± 50.2) was significantly lower compared with street track test (85.7 ± 94.3 and t = 2.78). BS did not show significant differences between tests and HR of horses was not always correlated with the observed moderate behavioral responses. HR, HRV, PPS and BS did not differ between repetition of tests and there were no significant differences in any of the four tests between experienced and inexperienced horses. No habituation occurred during the test weeks, and experience as a police horse does not seem to be a key factor in how these horses handle stress. All horses showed only modest behavioral responses, and HR may provide complimentary information for individual evaluation and welfare assessment of these horses. Overall, little evidence of stress was observed during these police training tests. As three of these tests (excluding the indoor arena test) reflect normal police work, it is suggested that this kind of police work is not significantly stressful for horses and will have no negative impact on the horse's welfare.
    Schmallenberg virus in Culicoides spp. biting midges, the Netherlands, 2011
    Elbers, A.R.W. ; Meiswinkel, R. ; Weezep, E. van; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Kooi, E.A. - \ 2013
    Emerging Infectious Diseases 19 (2013)1. - ISSN 1080-6040 - p. 106 - 109.
    ephemeral fever - culicoides - cattle - arthropods - isolations - europe
    To determine which species of Culicoides biting midges carry Schmallenberg virus (SBV), we assayed midges collected in the Netherlands during autumn 2011. SBV RNA was found in C. scoticus, C. obsoletus sensu stricto, and C. chiopterus. The high proportion of infected midges might explain the rapid spread of SBV throughout Europe. During early summer 2011, Schmallenberg virus (SBV), a novel orthobunyavirus of the Simbu serogroup, spread across much of northern Europe, infecting ruminant livestock. The Simbu serogroup (family Bunyaviridae, genus Bunyavirus) includes Shamonda virus, Akabane virus, Sathuperi virus, and Aino virus. These viruses cause teratologic effects in ruminants and are arthropod-borne, and most have been isolated in the Old World from mosquitoes and Culicoides spp. biting midges (1). Recent preliminary studies indicate that =1 species of Culicoides midges act as field vectors for SBV in Europe (2). To determine which Culicoides midge species harbor SBV, we analyzed midges collected from 3 livestock holdings in eastern and northeastern parts of the Netherlands.
    Culicoides obsoletus extract relevant for diagnostics of insect bite hypersensitivity in horses
    Meide, N.M.A. van der; Meulenbroeks, C. ; Altena, S.E.C. van; Schurink, A. ; Ducro, B.J. ; Wagner, B. ; Leibold, W. ; Rohwer, J. ; Jacobs, F. ; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Savelkoul, H.F.J. ; Tijhaar, E. - \ 2012
    Veterinary Immunology and Immunopathology 149 (2012)3-4. - ISSN 0165-2427 - p. 245 - 254.
    dermatitis sweet itch - dermal hypersensitivity - icelandic horses - british-columbia - summer eczema - equine ige - antibodies - ceratopogonidae - identification - netherlands
    Insect bite hypersensitivity (IBH) is an allergic dermatitis in horses caused by the bites of Culicoides species. The aim of the present study was to evaluate the applicability of whole body extracts of C. obsoletus (the main species found feeding on horses in the Netherlands), C. nubeculosus (rarely found in The Netherlands) and C. sonorensis (typical for North America) for diagnosis of IBH in horses in The Netherlands. Blood and serum samples of 10 clinically confirmed IBH affected and 10 healthy control horses were used to evaluate the IgE titers (ELISA) against the Culicoides whole body extracts of the three Culicoides species. Basophil degranulation was assessed by histamine release test (HRT) after stimulation with these extracts at 5, 0.5 and 0.05 µg/ml. IBH affected horses had significantly higher IgE titers against C. obsoletus than against C. nubeculosus and C. sonorensis. Furthermore, C. obsoletus induced significantly higher histamine release in whole blood of IBH affected horses compared to the other extracts at 0.5 µg/ml. Western blot data revealed IgE binding to many proteins in C. obsoletus extract. This interaction was absent or weak in C. nubeculosus and C. sonorensis extracts for IBH affected horses. Results on individual level indicate that the HRT is more sensitive than ELISA in diagnosing IBH. However, ELISA is more practical as a routine test, therefore the ELISA was further evaluated using C. obsoletus extract on 103 IBH affected and 100 healthy horses, which resulted in a test sensitivity and specificity of 93.2% and 90.0%, respectively. The IgE ELISA readings enabled the analysis of the predicted probability of being IBH affected. From an optical density 450 nm value of 0.33 onwards, the probability of IBH affected was more than 0.9. The results presented in this paper show that the use of native Culicoides spp. that feed on horse, is important for improved diagnosis and that the described ELISA based on C. obsoletus can be used routinely to diagnose IBH in countries where this species is the main Culicoides feeding on horses
    The influence of challenging objects and horse-rider matching on heart rate, heart rate variability and behavioural score in riding horses
    Munsters, C. ; Visser, E.K. ; Broek, J. van den; Sloet van Oldruitenborgh-Oosterbaan, M.M. - \ 2012
    The Veterinary Journal 192 (2012)1. - ISSN 1090-0233 - p. 75 - 80.
    young horses - mental stress - temperament - welfare - equitation - animals - parameters - responses - system - health
    A good horse-rider 'match' is important in the context of equine welfare. To quantify the influence of repetition and horse-rider matching on the stress of horses encountering challenging objects, 16 Warmblood horses were ridden in a test-setting on three occasions. On each occasion the horse was ridden by a different rider and was challenged by three objects (A-C). Heart rate (HR), heart rate variability (HRV) of horse and rider, and behaviour score (BS) of the horse were obtained for each object and as a total for each test. The horse-rider interaction was evaluated with each combination and assessed as 'matching' or 'mismatching', and the horses were categorised as 'compliant', 'partly-compliant' or 'non-compliant'. Horses exhibited a decreased HR (P=0.015) and a decreased BS (P=0.004) within and across different tests. 'Matching' horse-rider combinations exhibited less stress as indicated by reduced HR ('match' 69±10 vs. 'mismatch' 72±9, P=0.001) and BS ('match' 1.9±1.1 vs. 'mismatch' 3.8±1.4, P=0.017) of the horse. 'Compliant' (68±8, P
    Wat is Afrikaanse paardenpest?
    Anonymous, ; Sloet van Oldruitenborgh-Oosterbaan, M.M. - \ 2011
    horses - african horse sickness - horse diseases - vector-borne diseases
    Intradermal testing of horses with and without insect bite hypersensitivity in the Netherlands using an extract of native Culicoides species
    Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Poppel, M. van; Raat, I.J. de; Boom, R. van den; Savelkoul, H.F.J. - \ 2009
    Veterinary Dermatology 20 (2009)5-6. - ISSN 0959-4493 - p. 607 - 614.
    sweet itch - icelandic horses - british-columbia - summer eczema - challenge - ceratopogonidae - dermatitis - permethrin - diptera - norway
    Intradermal tests using a Culicoides nubeculosus extract have proven unreliable for diagnosis of equine insect bite hypersensitivity (IBH) in the Netherlands. The aim of this study was to evaluate the diagnostic value of an extract derived from the Dutch species C. obsoletus and C. pulicaris. Thirteen pairs of horses were tested, each pair consisting of one horse with IBH and the other a healthy control. Each horse was injected intradermally with 0.1 mL of three concentrations of Culicoides whole body extract (1 : 1000 w/v, 1 : 10,000 w/v and 1 : 25,000 w/v), histamine solution (0.01 mg/mL, positive control) and phosphate-buffered saline (negative control). Skin responses were evaluated after 30 min and at 1, 4 and 24 h. At all time points the absolute wheal diameter elicited by Culicoides extract 1 : 1000 w/v was significantly larger (P <0.01) in the IBH horses than in the control horses. Using the 1 : 10,000 w/v extract the difference was significant at 1, 4 and 24 h and using the 1 : 25,000 w/v extract only at 24 h. The relative wheal diameter was greater in IBH than in control horses at all concentrations and time points except at 0.5 h for the 1 : 10,000 w/v and 1 : 25,000 w/v concentrations. At the 1 : 1000 w/v concentration, the sensitivity and specificity was 92% using the relative wheal diameter. These results indicate that intradermal testing using 1 : 1000 w/v concentration Culicoides extracts relevant to the locality provides useful support for a clinical diagnosis of equine insect hypersensitivity
    'Emerging vector-borne diseases' bij het paard
    Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Goehring, L.S. ; Koopmans, M.P.G. ; Rijn, P.A. van; Maanen, C. van - \ 2009
    Tijdschrift voor Diergeneeskunde 134 (2009)10. - ISSN 0040-7453 - p. 439 - 447.
    Culicoides bij paarden in Nederland
    Sloet van Oldruitenborgh-Oosterbaan, M.M. - \ 2008
    [S.l. : S.n. - 45 p.
    paarden - paardenziekten - bluetonguevirus - virusziekten - bepaling - maatregelen - dierziektepreventie - horses - horse diseases - bluetongue virus - viral diseases - determination - measures - animal disease prevention
    Om een goed inzicht te krijgen in de diverse beschermingsmethoden voor paarden tegen Culicoides spp. is het van belang eerst een overzicht te hebben van wat Culicoides zijn, welke Culicoides spp. hier voorkomen en de wijze van vangen en determineren, en de levenscyclus inclusief locatie en tijdsduur van de diverse stadia. Ook is van belang welke kennis naar voren is gekomen vanuit de problematiek rondom blauwtong. Hierna worden de mogelijke maatregelen genoemd
    Culicoides species attracted to horses with and without insect hypersensitivity
    Rijt, R. van der; Boom, R. van den; Jongema, Y. ; Sloet van Oldruitenborgh-Oosterbaan, M.M. - \ 2008
    The Veterinary Journal 178 (2008)1. - ISSN 1090-0233 - p. 91 - 97.
    sweet-itch - intradermal challenge - icelandic horses - british-columbia - ceratopogonidae - diptera - extracts - trap - dermatitis - mosquitos
    The aims of this study were to determine (1) which species of Culicoides is most commonly attracted to horses, (2) whether horses suffering insect hypersensitivity attract more Culicoides spp. than unaffected horses, and (3) the times when Culicoides spp. are most active. Horses affected by insect hypersensitivity and unaffected horses were placed inside mosquito netting tents for 30 min at different times of the day. All Culicoides spp. trapped inside the tents were collected and identified. C. obsoletus was the most common species found, followed by C. pulicaris. Healthy horses attracted slightly more midges than horses that were affected with insect hypersensitivity. All of the Culicoides species were most active at sunset, less so at sunrise and very few or no midges were trapped in the afternoon or at night.
    Identification of factors associated with the development of insect bite hypersensitivity in horses in the Netherlands
    Boom, R. van den; Ducro, B.J. ; Sloet van Oldruitenborgh-Oosterbaan, M.M. - \ 2008
    Tijdschrift voor Diergeneeskunde 133 (2008)13. - ISSN 0040-7453 - p. 554 - 563.
    paardenziekten - zomereczeem - insectenbeten - allergieën voor geleedpotigen - overgevoeligheid - paarden - horse diseases - sweet itch - insect bites - arthropod allergies - hypersensitivity - horses - dermatitis sweet itch - british-columbia - culicoides hypersensitivity - dermal hypersensitivity - icelandic horses - genetic-basis - ceratopogonidae - diptera - israel - diseases
    An Internet-based questionnaire among horse owners was carried out to identify factors affecting the incidence of insect bite hypersensitivity (IBHI) among horses in the Netherlands. Information was obtained for 794 horses of various breeds, but the breed distribution was not representative for the Dutch horse population. Of the horses for which information was available, 56% suffered from IBH and 44% did not. The most common clinical symptoms were pruritus, scaling, and hair loss, occurring mainly at the base of the tail and along the mane. Breed, age, region (and local habitat), stabling, type of bedding in the stable, use of the horse, deworming frequency, and season were associated with significant differences in IBH incidence. Knowledge of the factors influencing the incidence of IBH may make it possible to reduce the number of animals affected and help alleviate symptoms in affected horses.
    Studies on the mechanism by which a high intake of soybean oil depresses the apparent digestibility of fibre in horses
    Jansen, W.L. ; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Cone, J.W. ; Vries, H.T. de; Hallebeek, J.M. ; Hovenier, R. ; Kuilen, J. van der; Huurdeman, C.M. ; Verstappen, D.C.G.M. ; Gresnigt, M.C. ; Beynen, A.C. - \ 2007
    Animal Feed Science and Technology 138 (2007)3-4. - ISSN 0377-8401 - p. 298 - 308.
    chain fatty-acids - intestinal microflora - bile-acids - inhibition - glucose
    An increased intake of soybean oil at the expense of an iso-energetic amount of non-structural carbohydrates reduces the apparent digestibility of fibre in horses. Literature data indicate that bile acids and linoleic acid (C18:2 n ¿ 6) may inhibit growth of pure cultures of microorganisms. In the present series of experiments, the hypotheses tested were that after extra fat intake as soybean oil more bile acids and linoleic acid would enter the caecum which depresses microbial growth and thus also fibre fermentation. Based on measurements of faecal bile acid excretion in horses, no evidence was obtained for a higher influx of bile acids into the caecum after iso-energetic substitution of dietary soybean oil for starch plus glucose. When dietary palm oil was replaced by soybean oil, which caused a six-fold increase in linoleic acid intake, fibre digestibility in horses was not lowered. The infusion of linoleic acid into the caecum of fistulated ponies increased apparent fibre digestibility. It is concluded that the results of the three experiments disprove the hypotheses tested.
    High fat intake by ponies reduces both apparent digestibility of dietary cellulose and cellulose fermentation by faeces and isolated caecal and colonic contents
    Jansen, W.L. ; Cone, J.W. ; Geelen, S.N.J. ; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Gelder, A.H. van; Oude Elferink, S.J.W.H. ; Beynen, A.C. - \ 2007
    Animal Feed Science and Technology 133 (2007)3-4. - ISSN 0377-8401 - p. 298 - 308.
    gas-production - in-vitro - fiber digestion - rumen - bacteria - kinetics - cecum
    Inclusion of extra fat in the diet of horses, at the expense of an iso-energetic amount of nonstructural carbohydrates, has been shown to decrease the apparent digestibility of the various dietary fiber fractions, but the mechanism was unknown. It was hypothesized that inclusion of extra fat in the diet depresses the caecal and/or colon microbial degradation of fiber, leading to a decrease in fiber digestion. The hypothesis was tested using six ponies fed either low fat or a high fat ration. In the ponies, the high fat ration lowered apparent NDFom digestibility by 0.089. The 12 ponies were killed and intestinal contents isolated to be used as inoculum with in vitro gas production technique. In general, groups mean maximum gas production from either cellulose or xylan by caecal, colon and faecal bacteria was lower when the ponies had been fed the high fat diet. Cumulative gas production by caecal fluid with xylan as substrate after 20 h of incubation was depressed when the donor animals had been fed the high fat diet. With cellulose as substrate, gas production by caecal contents was lowered by on average 20% after fat feeding of the ponies. There was a diet effect of cell-free caecal fluid on gas production from cellulose by a standard inoculum: fat feeding had an inhibitory impact. It is concluded that fat feeding in ponies inhibits microbial activity in the caecum, which in turn leads to a decrease in fiber digestibility. (c) 2006 Elsevier B.V. All rights reserved.
    Landscape planning for a brookvalley with several country-seats.
    Kuiper, J. ; Santema, R. ; Sloet van Oldruitenborgh, C.J.M. - \ 1990
    In: IFLA-yearbook Manila - p. 139 - 141.
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