Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Effect of temperature on the formation of microsclerotia of Verticillium dahliae
    Soesanto, L. ; Termorshuizen, A.J. - \ 2001
    Journal of Phytopathology 149 (2001). - ISSN 0931-1785 - p. 685 - 691.
    Microsclerotium formation by six isolates of Verticillium dahliae was studied at different temperatures both in vitro and in Arabidopsis thaliana. In vitro mycelial growth was optimal at 25°C, but microsclerotium formation was greatest at 20°C (two isolates) or 15-20°C (one isolate). Seedlings of A. thaliana were root-dipped in a conidial suspension, planted, and either placed at 5, 10, 15, or 25°C, or left at 20°C until the onset of senescence, after which some of the plants were placed at 5, 10, 15, or 25°C. The amount of microsclerotia per unit of shoot weight was assessed in relation to isolate and temperature. The optimal temperature for production of microsclerotia was 15-25°C. Two isolates each produced about 10 times more microsclerotia than each of the other four isolates. For these isolates, high R2adj.-values of 0.77 and 0.66 were obtained, with temperature and its square as highly significant (P < 0.001) independent variables. R2adj.-values for the other isolates varied between 0.28 and 0.39. Moving plants to different temperatures at the onset of senescence led to microsclerotial densities that were intermediate between densities on plants that had grown at constantly 20°C and plants grown at other temperatures. This suggests that vascular colonization rate and rate of microsclerotium formation are similarly affected by temperature. The senescence rate of plants appeared unimportant except for plants grown at 25°C, which showed the highest amounts of microsclerotia per unit of plant weight in the most rapidly senescing plants.
    Consistent control of verticillium wilt by combination of Pseudomonas fluorescens P60 and Talaromyces flavus
    Termorshuizen, A.J. ; Soesanto, L. ; Raaijmakers, J.M. - \ 2000
    In: Proceedings of the Fifth International PGPR Workshop. - Auburn : Auburn University Web Site,
    Consistent control of Verticillium wilt by combination of Pseudomonas fluorescens P60 and Talaromyces flavus
    Termorshuizen, A.J. ; Soesanto, L. ; Raaijmakers, J.M. - \ 2000
    In: Poster abstracts 5th international PGPR workshop, 30 October - 3 November 2000, Córdoba, Argentina Cordoba, Argentina : - p. P129 - P129.
    Een biotoets voor Verticillium dahliae
    Soesanto, L. ; Termorshuizen, A.J. - \ 2000
    Gewasbescherming 31 (2000)3. - ISSN 0166-6495 - p. 81 - 81.
    Ecology and biological control of Verticillium dahliae
    Soesanto, L. - \ 2000
    Agricultural University. Promotor(en): M.J. Jeger; A.J. Termorshuizen. - S.l. : S.n. - ISBN 9789058081926 - 120
    verticillium dahliae - plantenziekteverwekkende schimmels - plantenziekten - ecologie - biologische bestrijding - plantenziektebestrijding - talaromyces flavus - biotesten - arabidopsis thaliana - verticillium dahliae - plant pathogenic fungi - plant diseases - ecology - biological control - plant disease control - talaromyces flavus - bioassays - arabidopsis thaliana

    The dynamics of Verticillium dahliae , the causal agent of wilt disease in many crops including potato, cotton, and olive, were investigated. Its biological control with Talaromyces flavus with or without additional Pseudomonas fluorescens was attempted. Arabidopsis thaliana was selected as a bioassay plant for studying aspects of ecology and biological control of the pathogen because of its short life cycle and high sensitivity to the pathogen. The optimal temperature for production of microsclerotia, the survival structures of the pathogen, both in vitro and on A. thaliana was about 20°C. Microsclerotia incorporated in soil were exposed to a range of conditions of temperature and moisture and quantified on several sampling occasions. One day after incorporation, densities were low, these densities were even lower over the following 1-6 months, but subsequently densities increased. These changes were ascribed to changes in the level of soil mycostasis rather than death and new formation of microsclerotia. After application of T. flavus to fresh organic debris containing microsclerotia followed by a 3-wk incubation aboveground at 15 or 25°C the population density of T. flavus increased in soil, especially at 25°C. T. flavus significantly reduced the density of microsclerotia in soil, especially at 25°C, and delayed the development of senescence of A. thaliana at 15 and 25°C.

    It is concluded that above-ground application of T. flavus may lead to more consistent effects. The effect of P. fluorescens strain P60, originally isolated from a take-all decline field continuously grown to wheat, on V. dahliae was also studied. Strain P60, and two other isolates of P. fluorescens , inhibited the in vitro mycelial growth of 20 isolates of V. dahliae , reduced formation of microsclerotia both in vitro and on A. thaliana and they retarded senescence of A. thaliana to a rate like that of uninoculated plants.

    Antibiotica-producerende Pseudomonas spp. en biologische bestrijding
    Raaijmakers, J.M. ; Souza, J. de; Soesanto, L. ; Termorshuizen, A.J. - \ 2000
    Gewasbescherming 31 (2000)2. - ISSN 0166-6495 - p. 54 - 55.
    Formation of microsclerotia of Verticillium dahliae.
    Soesanto, L. ; Termorshuizen, A.J. - \ 1997
    In: In: Book of abstracts of the 8th International Verticillium Symposium, Athens, Greece - p. 33 - 33.
    Kamus Istilah Fitopatologi.
    Soesanto, L. - \ 1996
    Yogyakarta : Kanisius - 196 p.
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