Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Cloning and functional characterization of BcatrA, a gene encoding an ABC transporter of the plant pathogenic fungus Botryotinia fuckeliana (Botrytis cinerea)
    Sorbo, G. Del; Ruocco, M. ; Schoonbeek, H. ; Scala, F. ; Pane, C. ; Vinale, F. ; Waard, M.A. de - \ 2008
    Mycological Research 112 (2008)6. - ISSN 0953-7562 - p. 737 - 746.
    binding-cassette transporter - natural toxic compounds - saccharomyces-cerevisiae - multidrug-resistance - mycosphaerella-graminicola - drosophila-melanogaster - aspergillus-nidulans - efflux pump - cutinase-a - yeast
    BcatrA was cloned from the plant pathogenic fungus Botryotinia fuckeliana (Botrytis cinerea) and sequenced. Sequence analysis revealed that BcatrA encodes a protein composed of 1562 amino acid residues displaying high similarity with various fungal ATP-binding cassette (ABC) transporters having the (NBF-TM6)2 topology. Expression of BcatrA is barely detectable during normal vegetative growth in liquid substrates. Transcript levels of BcatrA are enhanced in a dose- and time-dependent manner after treatment with cycloheximide or catechol, but not by a number of other drugs or fungicides, including fludioxonil, fenarimol, imazalil, and the plant defense compounds pisatin and resveratrol. Quantitative analysis of BcatrA during the synchronized infection of bean leaves revealed an overaccumulation of the gene transcript at 6, 12 and 24 h post-inoculation, suggesting an involvement of the gene in the first steps of pathogenesis. Functional analysis of BcatrA was performed by targeted gene replacement in a wild-type strain of the fungus, and by overexpression in a mutant of Saccharomyces cerevisiae carrying multiple non-functional multidrug-resistance genes. BcatrA replacement mutants did not show any significant increase in sensitivity to drugs, including inducers of BcatrA transcription, and displayed an unaltered virulence on several common host plants of B. cinerea. However, when expressed in the heterologous system, BcatrA reduced sensitivity to cycloheximide and catechol, thus indicating the ability of the BcatrA product to function as a multidrug transporter.
    The ABC transporter BcatrB affects the sensitivity of Botrytis cinerea to the phytoalexin resveratrol and the fungicide fenpiclonil
    Schoonbeek, H. ; Sorbo, G. Del; Waard, M.A. De - \ 2001
    Molecular Plant-Microbe Interactions 14 (2001)4. - ISSN 0894-0282 - p. 562 - 571.
    During pathogenesis, fungal pathogens are exposed to a variety of fungitoxic compounds. This may be particularly relevant to Botrytis cinerea, a plant pathogen that has a broad host range and, consequently, is subjected to exposure to many plant defense compounds. In practice, the pathogen is controlled with fungicides belonging to different chemical groups. ATP-binding cassette (ABC) transporters might provide protection against plant defense compounds and fungicides by ATP-driven efflux mechanisms. To test this hypothesis, we cloned BcatrB, an ABC transporter-encoding gene from B. cinerea. This gene encodes a 1,439 amino acid protein with nucleotide binding fold (NBF) and transmembrane (TM) domains in a [NBFTM6]2 topology. The amino acid sequence has 31 to 67% identity with ABC transporters from various fungi. The expression of BcatrB is up regulated by treatment of B. cinerea germlings with the grapevine phytoalexin resveratrol and the fungicide fenpiclonil. BcatrB replacement mutants are not affected in saprophytic growth on different media but are more sensitive to resveratrol and fenpiclonil than the parental isolate. Furthermore, virulence of ΔBcatrB mutants on grapevine leaves was slightly reduced. These results indicate that BcatrB is a determinant in sensitivity of B. cinerea to plant defense compounds and fungicides.
    De rol van ABC transporters in bescherming van Botrytis cinerea tegen plantenafweerstoffen en fungiciden
    Schoonbeek, H.J. ; Sorbo, G. Del; Waard, M.A. de - \ 2000
    Gewasbescherming 31 (2000)2. - ISSN 0166-6495 - p. 59 - 59.
    Fungal transporters involved in efflux of natural toxic compounds and fungicides
    Sorbo, G. Del; Schoonbeek, H. ; Waard, M.A. de - \ 2000
    Fungal Genetics and Biology 30 (2000). - ISSN 1087-1845 - p. 1 - 15.
    Survival of microorganisms in natural environments is favored by the capacity to produce compounds toxic to competing organisms and the ability to resist the effects of such toxic compounds. Both factors contribute to a competitive advantage of organisms in ecosystems. All organisms have evolved active transport mechanisms by which endogenous and exogenous toxicants can be secreted. Two major classes of transporter proteins are the ATP-binding cassette (ABC) and the major facilitator superfamily (MFS) transporters. Members of both classes can have broad and overlapping substrate specificities for natural toxic compounds and can be regarded as a "first-line defense barrier" in survival mechanisms. In plant pathogens, these transporters can play an essential role in protection against plant defense compounds during pathogenesis. Also, some transporters actively secrete host-specific and non-host-specific toxins. Remarkably, ABC and MFS transporters can also play a major role in fungicide sensitivity and resistance. Their role in multidrug resistance of Aspergillus nidulans, Candida albicans, and Saccharomyces cerevisiae to azoles and other fungitoxic compounds is well established. Knowledge of ABC and MFS transporters opens possibilities of developing novel strategies for controlling plant diseases, either by modulation of transporter activity or by transgenic expression of transporter genes in plants.
    ABC and MFS transporters from Botrytis cinerea
    Waard, M.A. de; Sorbo, G. Del; Hayashi, K. ; Schoonbeek, H. ; Vermeulen, T. - \ 2000
    In: Book of Abstracts : XIIth International Botrytis Symposium, Reims, France, 3-7 July 2000 Reims, France : Université de Reims - p. L40 - L40.
    The ABC transporter AtrB from Aspergillus nidulans is involved in resistance to all major classes of fungicides and natural toxic compounds
    Andrade, A.C. ; Sorbo, G. Del; Nistelrooy, J.G.M. van; Waard, M.A. de - \ 2000
    Microbiology 146 (2000). - ISSN 1350-0872 - p. 1987 - 1997.
    This paper reports the functional characterization of AtrBp, an ABC transporter from Aspergillus nidulans. AtrBp is a multidrug transporter and has affinity to substrates belonging to all major classes of agricultural fungicides and some natural toxic compounds. The substrate profile of AtrBp was determined by assessing the sensitivity of deletion and overexpression mutants of atrB to several toxicants. All mutants showed normal growth as compared to control isolates. atrB mutants displayed increased sensitivity to anilinopyrimidine, benzimidazole, phenylpyrrole, phenylpyridylamine, strobirulin and some azole fungicides. Increased sensitivity to the natural toxic compounds camptothecin (alkaloid), the phytoalexin resveratrol (stilbene) and the mutagen 4-nitroquinoline oxide was also found. Overexpression mutants were less sensitive to a wide range of chemicals. In addition to the compounds mentioned above, decreased sensitivity to a broader range of azoles, dicarboximides, quintozene, acriflavine and rhodamine 6G was observed. Decreased sensitivity in overexpression mutants negatively correlated with levels of atrB expression. Interestingly, the overexpression mutants displayed increased sensitivity to dithiocarbamate fungicides, chlorothalonil and the iron-activated antibiotic phleomycin. Accumulation of the azole fungicide [14C]fenarimol by the overexpression mutants was lower as compared to the parental isolate, demonstrating that AtrBp acts by preventing intracellular accumulation of the toxicant. Various metabolic inhibitors increased accumulation levels of [14C]fenarimol in the overexpression mutants to wild-type levels, indicating that reduced accumulation of the fungicide in these mutants is due to increased energy-dependent efflux as a result of higher pump capacity of AtrBp.
    The role of ABC transporters in pathogenesis of Botrytis cinerea
    Schoonbeek, H. ; Sorbo, G. Del; Waard, M.A. de - \ 1999
    In: Modern fungicides and antifungal compounds II / Lyr, H., Russell, P.E., Dehne, H.W., Sisler, H.D., - p. 143 - 149.
    The role of BcatrB, a new ATP-binding cassette transporter, in pathogenesis of Botrytis cinerea
    Schoonbeek, H. ; Sorbo, G. Del; Waard, M.A. de - \ 1999
    Fungal Genetics Newsletter 46 (1999)supplement. - ISSN 0895-1942 - p. 115 - 115.
    The role of the ATP-binding cassette transporter BcatrB in pathogenesis of Botrytis cinerea
    Schoonbeek, H. ; Sorbo, G. Del; Waard, M. de - \ 1999
    In: Book of abstracts : 9th International Congress on Molecular Plant-Microbe Interactions. Amsterdam, the Netherlands. 25-30 July 1999. - [S.l.] : [s.n.], 1999 - p. 73 - 73.
    ABC transporters in Aspergillus nidulans
    Andrade, A.C. ; Nistelrooy, J.G.M. van; Sorbo, G. Del; Skatrud, P.L. ; Waard, M.A. de - \ 1999
    In: Abstract Book 2nd FEBS Advanced lecture course 'ATP-binding cassette transporters : from multidrug resistance to genetic disease', Gosau, Austria, 20-27 February 1999. - [S.l.] : [s.n.], 1999 - p. 70 - 70.
    Characterization of ABC transporters from Aspergillus nidulans
    Andrade, A.C. ; Nistelrooy, J.G.M. van; Sorbo, G. Del; Skatrud, P.L. ; Waard, M.A. de - \ 1999
    Fungal Genetics Newsletter 46 (1999)supplement. - ISSN 0895-1942 - p. 95 - 95.
    The role of ABC transporters in Aspergillus nidulans
    Andrade, A. ; Nistelrooy, J. van; Sorbo, G. Del; Skatrud, P. ; Waard, M. de - \ 1999
    In: Book of abstracts : 9th International Congress on Molecular Plant-Microbe Interactions, Amsterdam, the Netherlands, 25-30 July 1999. - [S.l.] : [s.n.], 1999 - p. 78 - 78.
    Potential for exploitation of ATP-binding cassette transporters in biological control
    Sorbo, G. Del; Ruocco, M. ; Lorito, M. ; Scala, F. ; Zoina, A. ; Andrade, A.C. ; Waard, M.A. de - \ 1998
    In: Molecular approaches in biological control / Duffy, B., Rosenberger, U., Defago, G., - p. 241 - 246.
    The role of ABC-transporters in pathogenesis of Botrytis cinerea.
    Schoonbeek, H. ; Sorbo, G. Del; Waard, M.A. de - \ 1998
    In: 12th International Reinhardsbrunn Symposium on Modern Fungicides and Antifungal Compounds, Friedrichroda, Germany (1998)
    Functional analysis of genes encoding ABC transporters from filamentous fungi by complementation of yeast PDR deficient mutants.
    Ruocco, M. ; Sorbo, G. Del; Decottignies, A. ; Andrade, A.C. ; Waard, M.A. de; Ziona, A. ; Scala, F. - \ 1998
    In: 4th European Conference on Fungal Genetics, Leon, Spain - p. 147 - 147.
    The role of bc-atrA: a gene encoding an ATP-binding cassette transporter, in multidrug resistance and pathogenicity of the plant pathogen Botrytis cinerea.
    Sorbo, G. del; Ruocco, M. ; Faretra, F. ; Scala, F. ; Waard, M.A. de - \ 1998
    In: 4th European Conference on Fungal Genetics, Leon, Spain - p. 242 - 242.
    Studies on the role of ABC transporters in multidrug resistance and pathogenicity of filamentous fungi.
    Sorbo, G. del; Ruocco, M. ; Andrade, A. ; Schoonbeek, H. ; Lorito, M. ; Zoina, A. ; Waard, M.A. de; Scala, F. - \ 1998
    In: 7th International Congress of Plant Pathology, Edinburgh, UK (1998) 5.5.7
    ABC Transporters in Aspergillus nidulans.
    Andrade, A.C. ; Xuei, X. ; Sorbo, G. Del; Skatrud, P.L. ; Waard, M.A. de - \ 1998
    In: 7th International Congress of Plant Pathology, Edinburgh, UK (1998) 5.5.13
    ABC transporters in Aspergillus nidulans.
    Andrade, A.C. ; Xuei, X. ; Sorbo, G. Del; Skatrud, P.L. ; Waard, M.A. de - \ 1998
    In: 4th European Conference on Fungal Genetics, Leon, Spain - p. 146 - 146.
    The role of ABC-transporters in plant pathogenesis of the fungus Botrytis cinerea.
    Schoonbeek, H. ; Sorbo, G. del; Waard, M.A. de - \ 1997
    In: Book of Abstracts FEBS Advanced Lecture Course ATP Binding Cassette (ABC) Transporters, Gosau, Austria - p. 90 - 90.
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