Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Non-structural carbohydrates in woody plants compared among laboratories
    Quentin, Audrey G. ; Pinkard, Elizabeth A. ; Ryan, Michael G. ; Tissue, David T. ; Baggett, Scott L. ; Adams, Henry D. ; Maillard, Pascale ; Marchand, Jacqueline ; Landhäusser, Simon M. ; Lacointe, André ; Gibon, Yves ; Anderegg, William R.L. ; Asao, Shinichi ; Atkin, Owen K. ; Bonhomme, Marc ; Claye, Caroline ; Chow, Pak S. ; Clément-Vidal, Anne ; Davies, Noel W. ; Dickman, Turin L. ; Dumbur, Rita ; Ellsworth, David S. ; Falk, Kristen ; Galiano, Lucía ; Grünzweig, José M. ; Hartmann, Henrik ; Hoch, Günter ; Hood, Sharon ; Jones, Joanna E. ; Koike, Takayoshi ; Kuhlmann, Iris ; Lloret, Francisco ; Maestro, Melchor ; Mansfield, Shawn D. ; Martínez-Vilalta, Jordi ; Maucourt, Mickael ; McDowell, Nathan G. ; Moing, Annick ; Muller, Bertrand ; Nebauer, Sergio G. ; Niinemets, Ülo ; Palacio, Sara ; Piper, Frida ; Raveh, Eran ; Richter, Andreas ; Rolland, Gaëlle ; Rosas, Teresa ; Joanis, Brigitte Saint ; Sala, Anna ; Smith, Renee A. ; Sterck, Frank ; Stinziano, Joseph R. ; Tobias, Mari ; Unda, Faride ; Watanabe, Makoto ; Way, Danielle A. ; Weerasinghe, Lasantha K. ; Wild, Birgit ; Wiley, Erin ; Woodruff, David R. - \ 2015
    Tree Physiology 35 (2015)11. - ISSN 0829-318X - p. 1146 - 1165.
    Extraction and quantification consistency - Non-structural carbohydrate chemical analysis - Particle size - Reference method - Soluble sugars - Standardization - Starch

    Non-structural carbohydrates (NSC) in plant tissue are frequently quantified to make inferences about plant responses to environmental conditions. Laboratories publishing estimates of NSC of woody plants use many different methods to evaluate NSC. We asked whether NSC estimates in the recent literature could be quantitatively compared among studies. We also asked whether any differences among laboratories were related to the extraction and quantification methods used to determine starch and sugar concentrations. These questions were addressed by sending sub-samples collected from five woody plant tissues, which varied in NSC content and chemical composition, to 29 laboratories. Each laboratory analyzed the samples with their laboratory-specific protocols, based on recent publications, to determine concentrations of soluble sugars, starch and their sum, total NSC. Laboratory estimates differed substantially for all samples. For example, estimates for Eucalyptus globulus leaves (EGL) varied from 23 to 116 (mean = 56) mg g-1 for soluble sugars, 6-533 (mean = 94) mg g-1 for starch and 53-649 (mean = 153) mg g-1 for total NSC. Mixed model analysis of variance showed that much of the variability among laboratories was unrelated to the categories we used for extraction and quantification methods (method category R2 = 0.05-0.12 for soluble sugars, 0.10-0.33 for starch and 0.01-0.09 for total NSC). For EGL, the difference between the highest and lowest least squares means for categories in the mixed model analysis was 33 mg g-1 for total NSC, compared with the range of laboratory estimates of 596 mg g-1. Laboratories were reasonably consistent in their ranks of estimates among tissues for starch (r = 0.41-0.91), but less so for total NSC (r = 0.45-0.84) and soluble sugars (r = 0.11-0.83). Our results show that NSC estimates for woody plant tissues cannot be compared among laboratories. The relative changes in NSC between treatments measured within a laboratory may be comparable within and between laboratories, especially for starch. To obtain comparable NSC estimates, we suggest that users can either adopt the reference method given in this publication, or report estimates for a portion of samples using the reference method, and report estimates for a standard reference material. Researchers interested in NSC estimates should work to identify and adopt standard methods.

    Ethical principles and social control: water distribution in the community of Morasloma, Ecaudor.
    Boelens, R. ; Caiza, S. ; Unda, R. - \ 1998
    In: Searching for equity: concepts of justice and equity in peasant irrigation / Boelens, R., Davila, G., Assen : Van Gorcum Publishers - p. 365 - 372.
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