Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Gastrointestinal digestion of dietary advanced glycation endproducts using an in vitro model of the gastrointestinal tract (TIM-1)
    Lugt, Timme van der; Venema, Koen ; Leeuwen, Stefan van; Vrolijk, Misha F. ; Opperhuizen, Antoon ; Bast, Aalt - \ 2020
    Food & Function 11 (2020)7. - ISSN 2042-6496 - p. 6297 - 6307.

    Protein- and sugar-rich food products processed at high temperatures contain large amounts of dietary advanced glycation endproducts (dAGEs). Our earlier studies have shown that specifically protein-bound dAGEs induce a pro-inflammatory reaction in human macrophage-like cells. To what extent these protein-bound dAGEs survive the human gastrointestinal (GI) tract is still unclear. In this study we analysed gastric and small intestinal digestion of dAGEs using the validated, standardised TNO in vitro gastroIntestinal digestion model (TIM-1), a dynamic in vitro model which mimics the upper human GI tract. This model takes multiple parameters into account, such as: dynamic pH curves, peristaltic mixing, addition of bile and pancreatic digestive enzymes, and passive absorption. Samples of different digested food products were collected at different time points after (i) only gastric digestion and (ii) after both gastric plus small intestinal digestion. Samples were analysed for dAGEs using UPLC-MS/MS for the lysine derived Nε-carboxymethyllysine (CML) and Nε-carboxyethyllysine (CEL), and the arginine derived methylglyoxal-derived hydroimidazolone-1 (MG-H1), and glyoxal-derived hydroimidazolone-1 (G-H1). All AGEs were quantified in their protein-bound and free form. The results of this in vitro study show that protein-bound dAGEs survive gastrointestinal digestion and are additionally formed during small intestinal digestion. In ginger biscuits, the presence MG-H1 in the GI tract increased with more than 400%. This also indicates that dAGEs enter the human GI tract with potential pro-inflammatory characteristics.

    Phase separation in biopolymer mixtures
    Dewi, Belinda Priska Chenty - \ 2020
    Wageningen University. Promotor(en): E. van der Linden, co-promotor(en): P. Venema. - Wageningen : Wageningen University - ISBN 9789463953535 - 193
    Effect of processing on the microstructure and composition of Bambara groundnut (Vigna subterranea (L.) Verdc.) seeds, flour and protein isolates
    Diedericks, Claudine F. ; Venema, Paul ; Mubaiwa, Juliet ; Jideani, Victoria A. ; Linden, Erik van der - \ 2020
    Food Hydrocolloids 108 (2020). - ISSN 0268-005X
    Bambara groundnut - Cryo-scanning electron microscopy - Legume processing - Microstructure - Multi-angle laser light scattering - Pulse protein

    Pre-processing treatments are generally employed to circumvent negating effects, such as the hard-to-cook and hard-to-mill properties, associated with legume seeds. Several studies have investigated the effects of soaking and roasting on the macroscopic qualities of Bambara groundnut (Vigna subterranea (L.) Verdc.) [BGN] seeds, yet knowledge is lacking on the effects of these treatments on the microstructural and molecular properties of the seeds and resulting fractions (i.e. flour and protein isolates). Size-exclusion chromatography coupled with light scattering results have shown that roasting induces thermal aggregation in BGN proteins, resulting in the formation of insoluble aggregates. To that end, the molecular composition of the roasted and soaked-roasted samples were comparable, yet different to the control (untreated seeds), whereas the samples which were subjected to soaking compared favourably to the control. The morphology of the seeds were also well characterized by microscopic techniques, including a confocal imaging technique which appears novel for legume seeds, revealing cotyledon cells with several starch granules embedded in a matrix of protein bodies. The findings of this study provide valuable insights on the microstructural and molecular compositional changes occurring in BGN seeds and fractions when subjected to soaking and/or roasting, which could be linked to some macroscopic properties as previously reported.

    Beyond a subsistence crop : Structure and functionality of Bambara groundnut proteins
    Diedericks, Claudine Florett - \ 2020
    Wageningen University. Promotor(en): E. van der Linden Linden, co-promotor(en): P. Venema; V.A. Jideani. - Wageningen : Wageningen University - ISBN 9789463953177 - 159

    Background and Aim

    In recent years, the importance of shifting from a largely animal-based protein diet to one rich in plant proteins have become increasingly apparent.  This is attributed to many factors of which most can be linked to aspects of food security, sustainability and climate change.  To that end, legume crops and specifically the so-called “neglected and underutilised species” are increasingly recognised as sources with the potential to address several of these factors important to the protein transition.  Bambara groundnut (Vigna subterranea (L.) Verdc.) [BGN] is one such a crop, which with a reported protein content of up to 27%, is increasingly recognised for its potential as an alternative plant protein source.  Research efforts have therefore been geared towards highlighting the functionality of BGN proteins, which although being of great value, are still lacking in terms of the link between the observed macroscopic functionality and the molecular and mesoscopic structural properties of the proteins; i.e. the structure-function relationship.  This is especially true for the gelation functionality of BGN proteins, which up till now have only been investigated for the minimum concentration above which a solid-like material can be observed after heating.  The aim of this thesis was therefore to gain insights into the structural properties of BGN proteins obtained from different processing streams and their resulting gelation properties.

    Methods

    To identify the interplay between processing, structure and functionality, BGN proteins of varying purity as obtained via different extraction routes were characterised for their physicochemical and structural properties, followed by characterisation of their gelation behaviour.  Hereto, techniques such as size-exclusion chromatography coupled to multi-angle laser light scattering provided detailed information on the molecular weight and size characteristics of the proteins, whilst microstructures were characterised with microscopic techniques (i.e. cryo-scanning electron microscopy and confocal laser scanning microscopy).  The gelling behaviour of the proteins were characterised in terms of their rheological responses and linked to scaling models, as a means of describing the resultant gel networks.  These techniques were also applied to evaluate the effect of pre-treatments (soaking and roasting) on the seed microstructures and composition of the resultant fractions, as well as to investigate the synergistic enhancement of BGN proteins in admixture with whey protein isolates.

    Results and Conclusions

    Vicilin proteins which are trimeric proteins (molecular weight around 150–170 kDa) were confirmed as the major storage proteins in BGN seeds with a high purity (>90% protein content); whilst also being present as the major component in the less pure BGN protein isolates.  The role of vicilin in the BGN protein isolates was evident in the gelation behaviour, where the rheological responses in terms of the elastic modulus G’ of the protein isolates, revealed a concentration dependence which closely resembled that of vicilin in the presence of 200 mM NaCl (conditions for highest solubility of BGN vicilin).  Furthermore, both BGN vicilin and BGN protein isolates were characterised as weak-link fractal structures, whilst microstructural images revealed inhomogeneous aggregates for both fractions.  These findings thus show that high purity BGN proteins (i.e. vicilin proteins) are not necessary to achieve the same functionality as the protein isolates with a lower purity.  Hence, the applicability of dry fractionation as a mild processing technique was also explored, which resulted in protein-enriched fractions with evident gel formation.  Branching out further on the processing aspect, it was observed that soaking as a pre-treatment method is beneficial to address the hard-to-cook properties associated with the seeds, whilst retaining a similar composition as that of the raw seeds.  Finally, BGN protein isolates also showed potential as partial animal protein replacers, based on the change in the rheological responses when combined with whey protein isolates.  Overall, the findings presented in this thesis provided new knowledge and thereby a better understanding of the structure-function behaviour of BGN proteins, which can be used to strengthen the position and steer the application of BGN as an alternative plant protein source.

    High Field MicroMRI Velocimetric Measurement of Quantitative Local Flow Curves
    Nikolaeva, Tatiana ; Vergeldt, Frank J. ; Serial, Raquel ; Dijksman, Joshua A. ; Venema, Paul ; Voda, Adrian ; Duynhoven, John Van; As, Henk Van - \ 2020
    Analytical Chemistry 92 (2020)6. - ISSN 0003-2700 - p. 4193 - 4200.

    Performing rheo-microMRI velocimetry at a high magnetic field with strong pulsed field gradients has clear advantages in terms of (chemical) sensitivity and resolution in velocities, time, and space. To benefit from these advantages, some artifacts need to be minimized. Significant sources of such artifacts are chemical shift dispersion due to the high magnetic field, eddy currents caused by the pulsed magnetic field gradients, and possible mechanical instabilities in concentric cylinder (CC) rheo-cells. These, in particular, hamper quantitative assessment of spatially resolved velocity profiles needed to construct local flow curves (LFCs) in CC geometries with millimeter gap sizes. A major improvement was achieved by chemical shift selective suppression of signals that are spectroscopically different from the signal of interest. By also accounting for imperfections in pulsed field gradients, LFCs were obtained that were virtually free of artifacts. The approach to obtain quantitative LFCs in millimeter gap CC rheo-MRI cells was validated for Newtonian and simple yield stress fluids, which both showed quantitative agreement between local and global flow curves. No systematic effects of gap size and rotational velocity on the viscosity of a Newtonian fluid and yield stress of a complex fluid could be observed. The acquisition of LFCs during heterogeneous and transient flow of fat crystal dispersion demonstrated that local constitutive laws can be assessed by rheo-microMRI at a high magnetic field in a noninvasive, quantitative, and real-time manner.

    Second order virial coefficients from phase diagrams
    Dewi, B.P.C. ; Linden, E. van der; Bot, Arjen ; Venema, P. - \ 2020
    Food Hydrocolloids 101 (2020). - ISSN 0268-005X - 16 p.
    critical point - Phase diagram - second virial coefficient
    The prediction of phase separation is essential to understand and control the properties of food systems. In this work, an existing theoretical model for describing phase separation between binary mixtures of hydrocolloids, using a virial approach up to second order, is extended with several new analytical expressions. These new expressions allow one to determine the three virial coefficients directly from three characteristics of the phase diagram, where the critical point plays a pivotal role and allows one to predict the complete phase diagram. The advantage of this approach is that experimental techniques, like membrane osmometry or static light scattering, to directly measure virial coefficients can be, in principle, avoided. It was found that just the location of the critical point is sufficient to determine two of the three virial coefficients, when one of the virial coefficients is known. When, in addition to the critical point, one other characteristic of the phase diagram is known with sufficient accuracy, like the slope of the tie-lines near or far away from the critical point, all three virial coefficients can be determined from the phase diagram. Using this approach, three virial coefficients for aqueous mixtures of dextran and polyethylene oxide were determined and compared to the ones obtained from membrane osmometry.
    Yellow pea aqueous fractionation increases the specific volume fraction and viscosity of its dispersions
    Kornet, Cornelis ; Venema, Paul ; Nijsse, Jaap ; Linden, Erik van der; Goot, Atze Jan van der; Meinders, Marcel - \ 2020
    Food Hydrocolloids 99 (2020). - ISSN 0268-005X
    Aqueous fractionation - Protein purification - Rarefied protein aggregates - Solubility - Viscosity - Yellow pea

    Some studies have shown that mild fractionation may result in similar or even better functional properties, than those of highly purified ingredients. This study aimed to relate the level of aqueous purification to the composition, solubility and viscosity of yellow pea fractions. A seldomly used method of cryo-planing combined with Cryo-SEM revealed the presence of protein bodies and starch granules in the seeds and flour, with sizes of ~3 μm and ~20 μm, respectively. Fractions with protein purities ranging from 40 to 85% (w/w) were obtained from the flour and characterized. These fractions were also compared to commerially available yellow pea protein isolate. The fractions that were only exposed to a solubilisation step contained high quantities of carbohydrates (23.6% w/w), which were mostly present as oligosaccharides. Subsequent fractionation steps increased the protein content and changed the ratio between the different pea proteins to some extent. We found that more fractionation steps reduced the solubility of the fractions. The most purified fraction contained 17% (w/w) insoluble protein aggregates with radii ≥ 100 nm. This fraction showed a substantial thickening capacity, with a viscosity of up to 103 mPa s at a concentration of 23% (w/w). The impurities (i.e. sugars, starch granules) present in the fractions only had a small effect on viscosity. Based on the protein specific volume fraction and particle size analysis, it was concluded that yellow pea protein can form aggregates with a rarefied structure responsible for its thickening capacity.

    Glastuinbouw: Barometer Duurzame landbouw Noord-Brabant
    Venema, G.S. ; Dolman, M.A. ; Smit, A.B. ; Jukema, G.D. ; Wisman, J.H. ; Jager, J.H. - \ 2019
    Wageningen Economic Research - 38 p.
    De factsheet Glastuinbouw maakt onderdeel uit van de Barometer Duurzame landbouw Noord-Brabant. In opdracht van de provincie Noord-Brabant wordt op basis van feiten over een breed terrein een zo actueel mogelijk beeld gegeven van de primaire agrarische sectoren. Ook wordt inzicht gegeven in opgetreden ontwikkelingen in de laatste decennia, en wordt een vergelijk gemaakt met de nationale ontwikkeling.
    Barometer Duurzame landbouw Noord-Brabant
    Venema, G.S. ; Dolman, M.A. ; Smit, A.B. ; Jukema, G.D. ; Wisman, J.H. ; Jager, J.H. - \ 2019
    Wageningen Economic Research - 32 p.
    Melkveehouderij: Barometer Duurzame landbouw Noord-Brabant
    Venema, G.S. ; Dolman, M.A. ; Smit, A.B. ; Jukema, G.D. ; Wisman, J.H. ; Jager, J.H. - \ 2019
    Wageningen Economic Research - 36 p.
    De factsheet Melkveehouderij maakt onderdeel uit van de Barometer Duurzame landbouw Noord-Brabant. In opdracht van de provincie Noord-Brabant wordt op basis van feiten over een breed terrein een zo actueel mogelijk beeld gegeven van de primaire agrarische sectoren. Ook wordt inzicht gegeven in opgetreden ontwikkelingen in de laatste decennia, en wordt een vergelijk gemaakt met de nationale ontwikkeling.
    Overig tuinbouw en blijvende teelt: Barometer Duurzame landbouw Noord-Brabant
    Venema, G.S. ; Dolman, M.A. ; Smit, A.B. ; Jukema, G.D. ; Wisman, J.H. ; Jager, J.H. - \ 2019
    Wageningen Economic Research - 29 p.
    De factsheet Overige tuinbouw en blijvende teelt maakt onderdeel uit van de Barometer Duurzame landbouw Noord-Brabant. In opdracht van de provincie Noord-Brabant wordt op basis van feiten over een breed terrein een zo actueel mogelijk beeld gegeven van de primaire agrarische sectoren. Ook wordt inzicht gegeven in opgetreden ontwikkelingen in de laatste decennia, en wordt een vergelijk gemaakt met de nationale ontwikkeling.
    Overige veehouderij: Barometer Duurzame landbouw Noord-Brabant
    Venema, G.S. ; Dolman, M.A. ; Smit, A.B. ; Jukema, G.D. ; Wisman, J.H. ; Jager, J.H. - \ 2019
    Wageningen Economic Research - 29 p.
    De factsheet Overige veehouderij maakt onderdeel uit van de Barometer Duurzame landbouw Noord-Brabant. In opdracht van de provincie Noord-Brabant wordt op basis van feiten over een breed terrein een zo actueel mogelijk beeld gegeven van de primaire agrarische sectoren. Ook wordt inzicht gegeven in opgetreden ontwikkelingen in de laatste decennia, en wordt een vergelijk gemaakt met de nationale ontwikkeling.
    Pluimveehouderij: Barometer Duurzame landbouw Noord-Brabant
    Venema, G.S. ; Dolman, M.A. ; Smit, A.B. ; Jukema, G.D. ; Wisman, J.H. ; Jager, J.H. - \ 2019
    Wageningen Economic Research - 34 p.
    De factsheet Pluimveehouderij maakt onderdeel uit van de Barometer Duurzame landbouw Noord-Brabant. In opdracht van de provincie Noord-Brabant wordt op basis van feiten over een breed terrein een zo actueel mogelijk beeld gegeven van de primaire agrarische sectoren. Ook wordt inzicht gegeven in opgetreden ontwikkelingen in de laatste decennia, en wordt een vergelijk gemaakt met de nationale ontwikkeling.
    Akkerbouw en vollegrondsgroenteteelt: Barometer Duurzame landbouw Noord-Brabant
    Venema, G.S. ; Dolman, M.A. ; Smit, A.B. ; Jukema, G.D. ; Wisman, J.H. ; Jager, J.H. - \ 2019
    Wageningen Economic Research - 43 p.
    De factsheet Akkerbouw en vollegrondsgroenteteelt maakt onderdeel uit van de Barometer Duurzame landbouw Noord-Brabant. In opdracht van de provincie Noord-Brabant wordt op basis van feiten over een breed terrein een zo actueel mogelijk beeld gegeven van de primaire agrarische sectoren. Ook wordt inzicht gegeven in opgetreden ontwikkelingen in de laatste decennia, en wordt een vergelijk gemaakt met de nationale ontwikkeling.
    Varkenshouderij: Barometer Duurzame landbouw Noord-Brabant
    Venema, G.S. ; Dolman, M.A. ; Smit, A.B. ; Jukema, G.D. ; Wisman, J.H. ; Jager, J.H. - \ 2019
    Wageningen Economic Research - 33 p.
    VarkenshouderijBarometer Duurzame landbouw Noord-Brabant | Wageningen Economic ResearchDe factsheet Varkenshouderij maakt onderdeel uit van de Barometer Duurzame landbouw Noord-Brabant. In opdracht van de provincie Noord-Brabant wordt op basis van feiten over een breed terrein een zo actueel mogelijk beeld gegeven van de primaire agrarische sectoren. Ook wordt inzicht gegeven in opgetreden ontwikkelingen in de laatste decennia, en wordt een vergelijk gemaakt met de nationale ontwikkeling.
    Boomkwekerij: Barometer Duurzame landbouw Noord-Brabant
    Venema, G.S. ; Dolman, M.A. ; Smit, A.B. ; Jukema, G.D. ; Wisman, J.H. ; Jager, J.H. - \ 2019
    Wageningen Economic Research - 24 p.
    De factsheet Boomkwekerij maakt onderdeel uit van de Barometer Duurzame landbouw Noord-Brabant. In opdracht van de provincie Noord-Brabant wordt op basis van feiten over een breed terrein een zo actueel mogelijk beeld gegeven van de primaire agrarische sectoren. Ook wordt inzicht gegeven in opgetreden ontwikkelingen in de laatste decennia, en wordt een vergelijk gemaakt met de nationale ontwikkeling.
    Yellow pea protein purification increases the viscosity of its dispersion
    Cornet, S.H.V. ; Venema, P. ; Goot, A.J. van der; Nijsse, J. ; Linden, E. van der; Meinders, M.B.J. - \ 2019
    Enhanced gelling capacity of yellow pea fractions through mild purification
    Cornet, S.H.V. ; Venema, P. ; Goot, A.J. van der; Linden, E. van der; Meinders, M.B.J. - \ 2019
    De landbouw en het landelijk gebied in Nederland in beeld : Een houtskoolschets van de SWOT voor het GLB
    Berkhout, Petra ; Doorn, Anne van; Geerling-Eiff, Floor ; Meulen, Harold van der; Tacken, Gemma ; Venema, Gabe ; Vogelzang, Theo - \ 2019
    Wageningen : Wageningen Economic Research (Wageningen Economic Research rapport 2019-058) - ISBN 9789463950947 - 103
    Voor de periode 2021-2027 moeten lidstaten in een Nationaal Strategisch Plan (NSP) uiteenzetten hoe zij met hun beleidsinzet gaan bijdragen aan door de Europese Commissie geformuleerde doelstellingen voor het Gemeenschappelijk Landbouwbeleid. Deze beleidsinzet is mede gebaseerd op een zogenaamde SWOT-analyse (Strengths Weaknesses Opportunities Threats). Met oog op het voorbereiden van het NSP, heeft het ministerie van LNV ervoor gekozen om een SWOT-analyse te maken in twee fasen. In de eerste fase wordt een SWOT-analyse op hoofdlijnen ('Houtskool-SWOT') opgesteld. In een tweede fase komt de definitieve SWOT-analyse tot stand. Dit rapport bevat de Houtskool-SWOT.
    Nulmeting omzet multifunctionele landbouw Regio Rivierenland
    Meulen, Harold van der; Vijn, Marcel ; Venema, Gabe - \ 2019
    Den Haag : Wageningen Economic Research (Wageningen Economic Research rapport 2019-029) - 16
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