Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Column: Wees niet bang om het met ons oneens te zijn
Vries, J.W. de - \ 2019
Bloemenkrant
‘Wees gastvrij voor de natuur’: de opiniemaker: Dick Melman
Melman, Dick - \ 2019

Dick Melman is onderzoeker Dierecologie bij Wageningen Universiteit en Researchcentrum. Hij weet dat Nederland het gebied bij uitstek is waar grutto's, scholeksters, kievieten en de tureluur gedijen. Als de maatschappij vindt dat we zuinig moeten zijn op de weidevogels, kan Nederland daar heel veel voor betekenen.

Combining QTL mapping with transcriptome and metabolome profiling reveals a possible role for ABA signaling in resistance against the cabbage whitefly in cabbage
Broekgaarden, Colette ; Pelgrom, Koen T.B. ; Bucher, Johan ; Dam, Nicole M. van; Grosser, Katharine ; Pieterse, Corné M.J. ; Kaauwen, Martijn van; Steenhuis, Greet ; Voorrips, Roeland E. ; Vos, Martin de; Vosman, Ben ; Worrich, Anja ; Wees, Saskia C.M. van - \ 2018
PLoS ONE 13 (2018)11. - ISSN 1932-6203

Whiteflies are among the world's most significant agricultural pests and chemical insecticides are extensively used to reduce crop damage to acceptable levels. However, nearly all insecticides pose a threat to the environment and alternative control methods, such as breeding of crop varieties that are inherently insect-resistant, are needed. Previously, a strong source of plant-age dependent resistance to the cabbage whitefly (Aleyrodes proletella) has been identified in the modern white cabbage (Brassica oleracea var. capitata) variety Rivera. However, nothing is known about the molecular mechanisms or the genes involved in this resistance. In the present study, a multidisciplinary approach combining transcriptome and metabolome profiling with genetic mapping was used to identify the molecular players of whitefly resistance in cabbage. Transcriptome profiles of young (susceptible) and older (resistant) Rivera plants were analyzed using RNA sequencing. While many genes involved in general processes were differentially expressed between both ages, several defense-related processes were overrepresented in the transcriptome profile of older plants. Hormone measurements revealed that jasmonic acid (JA) levels decreased upon whitefly infestation at both plant ages. Interestingly, abscisic acid (ABA) levels showed contrasting effects in response to whitefly infestation: ABA levels were reduced in young plants but induced in older plants upon whitefly feeding. Auxin levels were significantly lower in older plants compared with young plants, independent of whitefly presence, while glucosinolate levels were higher. Additionally, whitefly performance was monitored in an F2 population derived from a cross between Rivera and the susceptible white cabbage variety Christmas Drumhead. Significant QTL intervals were mapped on chromosome 2 and 9 for oviposition rate and whitefly adult survival, respectively. Several genes that were higher expressed in older plants and located in the identified QTL intervals were orthologous to Arabidopsis genes that have been related to ABA signaling, suggesting a role for ABA in the regulation of resistance towards whiteflies. Our results show that combining different omics approaches is a useful strategy to identify candidate genes underlying insect resistance.

Fieldlab 5G laat innovatie met drones zien
Hoekzema, Gerard ; Booij, Johan ; Veldhuisen, Bram - \ 2018
Genome-wide association study reveals novel players in defense hormone crosstalk in Arabidopsis
Proietti, Silvia ; Caarls, Lotte ; Coolen, Silvia ; Pelt, Johan A. van; Wees, Saskia C.M. van; Pieterse, Corné M.J. - \ 2018
Plant, Cell & Environment 41 (2018)10. - ISSN 0140-7791 - p. 2342 - 2356.
Jasmonic acid (JA) regulates plant defenses against necrotrophic pathogens and insect herbivores. Salicylic acid (SA) and abscisic acid (ABA) can antagonize JA‐regulated defenses, thereby modulating pathogen or insect resistance. We performed a genome‐wide association (GWA) study on natural genetic variation in Arabidopsis thaliana for the effect of SA and ABA on the JA pathway. We treated 349 Arabidopsis accessions with methyl JA (MeJA), or a combination of MeJA and either SA or ABA, after which expression of the JA‐responsive marker gene PLANT DEFENSIN1.2 (PDF1.2) was quantified as a readout for GWA analysis. Both hormones antagonized MeJA‐induced PDF1.2 in the majority of the accessions but with a large variation in magnitude. GWA mapping of the SA‐ and ABA‐affected PDF1.2 expression data revealed loci associated with crosstalk. GLYI4 (encoding a glyoxalase) and ARR11 (encoding an Arabidopsis response regulator involved in cytokinin signalling) were confirmed by T‐DNA insertion mutant analysis to affect SA–JA crosstalk and resistance against the necrotroph Botrytis cinerea. In addition, At1g16310 (encoding a cation efflux family protein) was confirmed to affect ABA–JA crosstalk and susceptibility to Mamestra brassicae herbivory. Collectively, this GWA study identified novel players in JA hormone crosstalk with potential roles in the regulation of pathogen or insect resistance.
Thrips advisor : Exploiting thrips-induced defences to combat pests on crops
Steenbergen, Merel ; Abd-El-Haliem, Ahmed ; Bleeker, Petra ; Dicke, Marcel ; Escobar-Bravo, Rocio ; Cheng, Gang ; Haring, Michel A. ; Kant, Merijn R. ; Kappers, Iris ; Klinkhamer, Peter G.L. ; Leiss, Kirsten A. ; Legarrea, Saioa ; Macel, Mirka ; Mouden, Sanae ; Pieterse, Corné M.J. ; Sarde, Sandeep J. ; Schuurink, Robert C. ; Vos, Martin De; Wees, Saskia C.M. Van; Broekgaarden, Colette - \ 2018
Journal of Experimental Botany 69 (2018)8. - ISSN 0022-0957 - p. 1837 - 1848.
Cell-content feeder - effectors - herbivorous insect - phytohormone signalling - plant defence - specialized metabolites - thrips - virus - volatiles

Plants have developed diverse defence mechanisms to ward off herbivorous pests. However, agriculture still faces estimated crop yield losses ranging from 25% to 40% annually. These losses arise not only because of direct feeding damage, but also because many pests serve as vectors of plant viruses. Herbivorous thrips (Thysanoptera) are important pests of vegetable and ornamental crops worldwide, and encompass virtually all general problems of pests: they are highly polyphagous, hard to control because of their complex lifestyle, and they are vectors of destructive viruses. Currently, control management of thrips mainly relies on the use of chemical pesticides. However, thrips rapidly develop resistance to these pesticides. With the rising demand for more sustainable, safer, and healthier food production systems, we urgently need to pinpoint the gaps in knowledge of plant defences against thrips to enable the future development of novel control methods. In this review, we summarize the current, rather scarce, knowledge of thrips-induced plant responses and the role of phytohormonal signalling and chemical defences in these responses. We describe concrete opportunities for breeding resistance against pests such as thrips as a prototype approach for next-generation resistance breeding.

More roses for less: Balancing between crop growth, fungal diseases and energy use in greenhouses
Westreenen, A. van; Evers, J.B. ; Anten, N.P.R. ; Wees, S. van; Hofland-Zijlstra, J. ; Marcelis, L.F.M. - \ 2018
'Wees kritisch op inzet schimmel- en bacteriepreparaten'
Boer, Wietse de; Korthals, Gerard - \ 2017
'Akkerbouwer, wees kritisch op bodemverbeteraars'
Haan, Janjo de - \ 2017
Genetic architecture of plant stress resistance: multi-trait genome-wide association mapping
Thoen, H.P.M. ; Davila Olivas, N.H. ; Kloth, K.J. ; Coolen, Silvia ; Huang, P. ; Aarts, M.G.M. ; Molenaar, J.A. ; Bakker, J. ; Bouwmeester, H.J. ; Broekgaarden, C. ; Bucher, J. ; Busscher-Lange, J. ; Cheng, X. ; Dijk-Fradin, E.F. van; Jongsma, M.A. ; Julkowska, Magdalena M. ; Keurentjes, J.J.B. ; Ligterink, W. ; Pieterse, Corné M.J. ; Ruyter-Spira, C.P. ; Smant, G. ; Schaik, C.C. van; Wees, Saskia C.M. van; Visser, R.G.F. ; Voorrips, R.E. ; Vosman, B. ; Vreugdenhil, D. ; Warmerdam, S. ; Wiegers, G.L. ; Heerwaarden, J. van; Kruijer, W.T. ; Eeuwijk, F.A. van; Dicke, M. - \ 2017
New Phytologist 213 (2017)3. - ISSN 0028-646X - p. 1346 - 1362.
Plants are exposed to combinations of various biotic and abiotic stresses, but stress responses are usually investigated for single stresses only. Here, we investigated the genetic architecture underlying plant responses to 11 single stresses and several of their combinations by phenotyping 350 Arabidopsis thaliana accessions. A set of 214 000 single nucleotide polymorphisms (SNPs) was screened for marker-trait associations in genome-wide association (GWA) analyses using tailored multi-trait mixed models. Stress responses that share phytohormonal signaling pathways also share genetic architecture underlying these responses. After removing the effects of general robustness, for the 30 most significant SNPs, average quantitative trait locus (QTL) effect sizes were larger for dual stresses than for single stresses. Plants appear to deploy broad-spectrum defensive mechanisms influencing multiple traits in response to combined stresses. Association analyses identified QTLs with contrasting and with similar responses to biotic vs abiotic stresses, and below-ground vs above-ground stresses. Our approach allowed for an unprecedented comprehensive genetic analysis of how plants deal with a wide spectrum of stress conditions.
Wees kien op rupsenschade in maïs
Rozen, K. van - \ 2016
Verantwoorde veehouderij
Wees kritisch tegenover Bodemverbeteraars
Os, Gera van - \ 2016
Transcriptome dynamics of Arabidopsis during sequential biotic and abiotic stresses
Coolen, Silvia ; Proietti, Silvia ; Hickman, Richard ; Davila Olivas, Nelson H. ; Huang, Pingping ; Verk, Marcel C. van; Pelt, Johan A. van; Wittenberg, Alexander H.J. ; Vos, Martin de; Prins, Marcel ; Loon, Joop J.A. van; Aarts, Mark G.M. ; Dicke, Marcel ; Pieterse, Corné M.J. ; Wees, Saskia C.M. van - \ 2016
The Plant Journal 86 (2016)3. - ISSN 0960-7412 - p. 249 - 267.
Arabidopsis thaliana - Botrytis cinerea - combinatorial plant stress - drought stress - gene regulatory network - Pieris rapae - plant hormones - RNA-Seq - transcript profiling - 016-3950

In nature, plants have to cope with a wide range of stress conditions that often occur simultaneously or in sequence. To investigate how plants cope with multi-stress conditions, we analyzed the dynamics of whole-transcriptome profiles of Arabidopsis thaliana exposed to six sequential double stresses inflicted by combinations of: (i) infection by the necrotrophic fungus Botrytis cinerea, (ii) herbivory by chewing larvae of Pieris rapae, and (iii) drought stress. Each of these stresses induced specific expression profiles over time, in which one-third of all differentially expressed genes was shared by at least two single stresses. Of these, 394 genes were differentially expressed during all three stress conditions, albeit often in opposite directions. When two stresses were applied in sequence, plants displayed transcriptome profiles that were very similar to the second stress, irrespective of the nature of the first stress. Nevertheless, significant first-stress signatures could be identified in the sequential stress profiles. Bioinformatic analysis of the dynamics of co-expressed gene clusters highlighted specific clusters and biological processes of which the timing of activation or repression was altered by a prior stress. The first-stress signatures in second stress transcriptional profiles were remarkably often related to responses to phytohormones, strengthening the notion that hormones are global modulators of interactions between different types of stress. Because prior stresses can affect the level of tolerance against a subsequent stress (e.g. prior herbivory strongly affected resistance to B. cinerea), the first-stress signatures can provide important leads for the identification of molecular players that are decisive in the interactions between stress response pathways.

Maatregelen voor betere bodem
Russchen, Harm Jan - \ 2016
Effect of prior drought and pathogen stress on Arabidopsis transcriptome changes to caterpillar herbivory
Davila Olivas, Nelson H. ; Coolen, Silvia ; Huang, Pingping ; Severing, Edouard ; Verk, Marcel C. van; Hickman, Richard ; Wittenberg, Alexander H.J. ; Vos, Martin de; Prins, Marcel ; Loon, Joop J.A. van; Aarts, Mark G.M. ; Wees, Saskia C.M. van; Pieterse, Corné M.J. ; Dicke, Marcel - \ 2016
New Phytologist 210 (2016)4. - ISSN 0028-646X - p. 1344 - 1356.
Abiotic stress - Botrytis cinerea - Combined stresses - Insect herbivory - Multiple stresses - Pieris rapae - RNAseq - Transcriptome - 016-3939

In nature, plants are exposed to biotic and abiotic stresses that often occur simultaneously. Therefore, plant responses to combinations of stresses are most representative of how plants respond to stresses. We used RNAseq to assess temporal changes in the transcriptome of Arabidopsis thaliana to herbivory by Pieris rapae caterpillars, either alone or in combination with prior exposure to drought or infection with the necrotrophic fungus Botrytis cinerea. Pre-exposure to drought stress or Botrytis infection resulted in a significantly different timing of the caterpillar-induced transcriptional changes. Additionally, the combination of drought and P. rapae induced an extensive downregulation of A. thaliana genes involved in defence against pathogens. Despite a more substantial growth reduction observed for plants exposed to drought plus P. rapae feeding compared with P. rapae feeding alone, this did not affect weight increase of this specialist caterpillar. Plants respond to combined stresses with phenotypic and transcriptional changes that differ from the single stress situation. The effect of a previous exposure to drought or B. cinerea infection on transcriptional changes to caterpillars is largely overridden by the stress imposed by caterpillars, indicating that plants shift their response to the most recent stress applied.

De permanente groene revolutie van Swaminathan
Fresco, L.O. ; Rabbinge, R. - \ 2015
Vork 2 (2015)3. - ISSN 2352-2925 - p. 66 - 71.
green revolution - genetic engineering - human feeding - hunger - food supply - scientific research - scientists - society - fertilizers - pesticides - environmental impact - india - netherlands - potatoes - wheat - groene revolutie - genetische modificatie - humane voeding - honger - voedselvoorziening - wetenschappelijk onderzoek - wetenschappers - samenleving - kunstmeststoffen - pesticiden - milieueffect - nederland - aardappelen - tarwe
Het weekblad Time kwalificeerde hem als een van de twintig meest invloedrijke Aziaten van de twintigste eeuw: ‘The father of the Green Revolution used his skills in genetic engineering and his powers of persuasion to make famine an unfamiliar word in Asia’. Tegelijkertijd wees hij al vroeg op de gevaren van een te grote afhankelijkheid van kunstmest en bestrijdingsmiddelen en milieugevolgen daarvan. Dr. Monkombu Sambasivan Swaminathan is een fervent pleitbezorger van de Evergreen Revolution, de permanente groene revolutie. Op 7 augustus werd hij 90 jaar. Louise Fresco en Rudy Rabbinge schetsen zijn enorme betekenis voor wetenschap en samenleving.
Opties voor beter mest- en gewasmanagement : verkenning op vijf Koeien & Kansenmelkveebedrijven
Verloop, J. ; Oenema, J. - \ 2014
Lelystad : Koeien & Kansen (Rapport / Plant Research International nr. 504) - 24
bemesting - dierlijke meststoffen - stikstof - fosfaat - koeien - melkvee - melkveehouderij - gevalsanalyse - fertilizer application - animal manures - nitrogen - phosphate - cows - dairy cattle - dairy farming - case studies
Dit verslag vloeit voort uit onderzoek naar de landbouwkundige en milieukundige effecten van mestscheiding op melkveebedrijven. De belangrijkste vraag in het onderzoek was in hoeverre mestscheiding bijdraagt aan het verhogen van de benutting van stikstof en fosfaat uit dierlijke mest door gewassen. Het onderzoek wees uit dat het succes van mestscheiding behalve van de technische uitvoering van de scheiding mogelijk ook afhankelijk is van aan mestmanagement gerelateerde maatregelen zoals opslag, aanwending en verdeling van mest. Dit vraagt om een analyse van mestmanagement op bedrijven waarin ook andere aspecten dan mestscheiding in beeld komen. Ook de werkwijze bij de ruwvoerteelt kan een rol spelen. In deze rapportage wordt deze mogelijkheid verkend.
Induced plant responses to microbes and insects
Pieterse, C.M.J. ; Poelman, E.H. ; Wees, S.C.M. van; Dicke, M. - \ 2013
Frontiers in Plant Science 4 (2013). - ISSN 1664-462X - 3 p.
induced resistance - arabidopsis - volatiles - susceptibility - pathways - immunity - defense - stress - growth - acid
Plants are members of complex communities and interact both with antagonists and beneficial organisms. An important question in plant defense-signaling research is how plants integrate signals induced by pathogens, insect herbivores and beneficial microbes into the most appropriate adaptive response. Molecular and genomic tools are now being used to uncover the complexity of the induced defense signaling networks that have evolved during the arms races between plants and the other organisms with which they intimately interact. To understand the functioning of the complex defense signaling network in nature, molecular biologists and ecologists have joined forces to place molecular mechanisms of induced plant defenses in an ecological perspective. In this Research Topic, we aim to provide an on-line, open-access snapshot of the current state of the art of the field of induced plant responses to microbes and insects, with a special focus on the translation of molecular mechanisms to ecology and vice versa. We will collect Original Research and Review papers on the topic, but also other article types, such as Methods and Opinions are welcome.
Molecular mechanism behind cross-talk between salicylic acid- and jasmonic acid-dependent pathways in Arabidopsis
Caarls, L. ; Does, D. van der; Boshoven, J.C. ; Wees, S. van; Pieterse, C. - \ 2013
In: Book of Abstracts 7th EPS Summerschool on Environmental Signaling, Utrecht, The Netherlands, 26-28 August 2013. - - p. 19 - 20.
Wees Erwinia de baas: Aanbevelingen tot beheersing
Vreeburg, P.J.M. ; Doorn, J. van; Leeuwen, P.J. van - \ 2013
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