Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Impact of human activities on the reproduction of Hooded Vultures Necrosyrtes monachus in Burkina Faso
    Daboné, Clément ; Buij, Ralph ; Oueda, Adama ; Adjakpa, Jacques Boko ; Guenda, Wendengoudi ; Weesie, Peter D.M. - \ 2019
    Ostrich 90 (2019)1. - ISSN 0030-6525 - p. 53 - 61.
    Burkina Faso - conservation - Hooded Vulture - human impact - reproduction

    During the last decades, the critically endangered Hooded Vulture Necrosyrtes monachus has strongly declined across its African range. Although direct persecution has been suggested as a major cause of this decline, little is known about the impact of humans on reproductive output in West Africa. We studied the impact of human activities on the reproductive output of Hooded Vultures in the Garango area of Burkina Faso. Twenty and 56 nesting attempts were monitored, respectively, during the breeding season in 2013/14 and 2014/15, to determine reproductive success and identify causes of nest failure. Annual breeding success varied between 0.68 and 0.71 chicks fledged per breeding pair per year and productivity was assessed at 0.57 chicks fledged per territorial pair in 2014/15. The main threats imposed by humans were poaching of eggs, chicks and collection of nest materials, leading to 20% (13 out of 64 breeding attempts) of nest failures over the two years. An additional important reason for nest failure was the pruning and (partial) cutting of nest trees. Despite this high level of human interference, we found that Hooded Vulture nest success increased with proximity to human settlements, probably because breeding vultures benefit from protection by people against persecution and disturbance.

    Phénologie de la reproduction du Vautour charognard Necrosyrtes monachus en zone soudano-sahélienne (Garango, Burkina Faso), 2013–2015
    Daboné, Clément ; Oueda, Adama ; Adjakpa, J.B. ; Buij, R. ; Ouedraogo, I. ; Guenda, W. ; Weesie, Peter D.M. - \ 2016
    Malimbus 38 (2016). - ISSN 0331-3689 - p. 38 - 49.
    Twenty nests of the Hooded Vulture Necrosyrtes monachus at Garango, east-central Burkina Faso, were regularly visited (mean 16 visits per nest) during the breeding period from 8 October 2013 to 15 May 2014, to determine the reproductive phenology. During the following breeding season (2014–15) 56 nests were studied to confirm the results obtained the previous season. Most nests were re-used old ones. During the 2013–14 breeding season, pairing and nest building were observed from the end of September 2013. The first clutches were observed from 30 October, with most laid in November and December 2013. In the 13 successful nests, hatching occurred after 45–52 days of incubation. Brooding of the 13 young which eventually flew lasted 3–4 months. During the 2014–15 breeding season, the 45 breeding pairs arrived in the breeding area from September 2014, 41 of the 45 clutches were laid before 28 December 2014, 31 of the 37 clutches which hatched did so before 31 January 2015, 26 of the 33 broods which flew did so before 3 May 2015 and the seven others before 20 May. These results confirm in most respects earlier studies in West and East Africa.
    Design and self-assembly of simple coat proteins for artificial viruses
    Hernandez Garcia, A. ; Kraft, D.J. ; Janssen, A.F.J. ; Bomans, P.H.H. ; Sommerdijk, N.A.J.M. ; Thies-Weesie, D.M.E. ; Favretto, M.E. ; Brock, R. ; Wolf, F.A. de; Werten, M.W.T. ; Schoot, P. van der; Cohen Stuart, M.A. ; Vries, R.J. de - \ 2014
    Nature Nanotechnology 9 (2014). - ISSN 1748-3387 - p. 698 - 702.
    tobacco-mosaic-virus - gene delivery - computational design - dna - cooperativity - copolymers - particle - cells
    Viruses are among the simplest biological systems and are highly effective vehicles for the delivery of genetic material into susceptible host cells1. Artificial viruses can be used as model systems for providing insights into natural viruses and can be considered a testing ground for developing artificial life. Moreover, they are used in biomedical and biotechnological applications, such as targeted delivery of nucleic acids for gene therapy1, 2 and as scaffolds in material science3, 4, 5. In a natural setting, survival of viruses requires that a significant fraction of the replicated genomes be completely protected by coat proteins. Complete protection of the genome is ensured by a highly cooperative supramolecular process between the coat proteins and the nucleic acids, which is based on reversible, weak and allosteric interactions only6, 7, 8, 9. However, incorporating this type of supramolecular cooperativity into artificial viruses remains challenging10, 11, 12, 13, 14, 15. Here, we report a rational design for a self-assembling minimal viral coat protein based on simple polypeptide domains. Our coat protein features precise control over the cooperativity of its self-assembly with single DNA molecules to finally form rod-shaped virus-like particles. We confirm the validity of our design principles by showing that the kinetics of self-assembly of our virus-like particles follows a previous model developed for tobacco mosaic virus9. We show that our virus-like particles protect DNA against enzymatic degradation and transfect cells with considerable efficiency, making them promising delivery vehicles.
    Orvelte bekeken; een onderzoek naar de achtergronden, motieven en waardering van de bezoekers van het monumentendorp Orvelte.
    Weesie, H. - \ 1986
    Unknown Publisher - 135 p.
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