Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Long-term impact of oral vancomycin, ciprofloxacin and metronidazole on the gut microbiota in healthy humans
Haak, Bastiaan W. ; Lankelma, Jacqueline M. ; Hugenholtz, Floor ; Belzer, Clara ; Vos, Willem M. de; Wiersinga, W.J. - \ 2019
Journal of Antimicrobial Chemotherapy 74 (2019)3. - ISSN 0305-7453 - p. 782 - 786.

OBJECTIVES: The impact of combination antibiotic therapy on the composition of the intestinal microbiota remains ill-defined. We aimed to assess the effect of a 1 week antibiotic regimen on the intestinal microbiota of healthy humans for a period of up to 31 months. PATIENTS AND METHODS: Thirteen healthy adult men received either no treatment or oral broad-spectrum antibiotics (ciprofloxacin, vancomycin and metronidazole) for 7 days. At four timepoints (prior to treatment, on day 9, day 49 and 8-31 months later) faecal samples were collected and analysed using 16S RNA gene sequencing. RESULTS: The short-term impact of broad-spectrum antibiotics on the gut microbiota was profound, with a loss of diversity and drastic shifts in community composition. In addition, antibiotics significantly reduced the abundance of bacterial taxa with important metabolic functions, such as the production of butyrate. The microbiota showed a remarkable return towards baseline after 8-31 months, but community composition often remained altered from its initial state. CONCLUSIONS: These findings suggest that combined treatment with vancomycin, ciprofloxacin and metronidazole has a profound and long-lasting effect on microbiota composition, the consequences of which remain largely unknown.

Rotavirus vaccine response correlates with the infant gut microbiota composition in Pakistan
Harris, Vanessa C. ; Ali, Asad ; Fuentes, Susana ; Korpela, Katri ; Kazi, Momin ; Tate, Jacqueline ; Parashar, Umesh ; Wiersinga, W.J. ; Giaquinto, Carlo ; Weerth, Carolina de; Vos, Willem M. de - \ 2018
Gut Microbes 9 (2018)2. - ISSN 1949-0976 - p. 93 - 101.
intestinal microbes - rotavirus vaccine - seroconversion - vaccine immunogenicity

Rotavirus (RV) is the leading cause of diarrhea-related death in children worldwide and ninety-five percent of rotavirus deaths occur in Africa and Asia. Rotavirus vaccines (RVV) can dramatically reduce RV deaths, but have low efficacy in low-income settings where they are most needed. The intestinal microbiome may contribute to this decreased RVV efficacy. This pilot study hypothesizes that infants' intestinal microbiota composition correlates with RVV immune responses and that RVV responders have different gut microbiota as compared to non-responders. We conducted a nested, matched case-control study comparing the pre-vaccination intestinal microbiota composition between 10 6-week old Pakistani RVV-responders, 10 6-week old Pakistani RVV non-responders, and 10 healthy Dutch infants. RVV response was defined as an Immunoglobulin A of ≥20 IU/mL following Rotarix™(RV1) vaccination in an infant with a pre-vaccination IgA<20. Infants were matched in a 1:1 ratio using ranked variables: RV1 dosing schedule (6/10/14; 6/10; or 10/14 weeks), RV season, delivery mode, delivery place, breastfeeding practices, age and gender. Fecal microbiota analysis was performed using a highly reproducible phylogenetic microarray. RV1 response correlated with a higher relative abundance of bacteria belonging to Clostridium cluster XI and Proteobacteria, including bacteria related to Serratia and Escherichia coli. Remarkably, abundance of these Proteobacteria was also significantly higher in Dutch infants when compared to RV1-non-responders in Pakistan. This small but carefully matched study showed the intestinal microbiota composition to correlate with RV1 seroconversion in Pakistan infants, identifying signatures shared with healthy Dutch infants.

Significant Correlation Between the Infant Gut Microbiome and Rotavirus Vaccine Response in Rural Ghana
Harris, Vanessa C. ; Armah, George ; Fuentes Enriquez de Salamanca, Susana ; Korpela, Katri E. ; Parashar, Umesh ; Victor, John C. ; Tate, Jacqueline ; Weerth, Carolina de; Giaquinto, Carlo ; Wiersinga, Willem Joost ; Lewis, Kristen D.C. ; Vos, Willem M. de - \ 2017
The Journal of Infectious Diseases 215 (2017)1. - ISSN 0022-1899 - p. 34 - 41.
intestinal microbiome - mucosal immunity - rotavirus vaccine

BACKGROUND:  Rotavirus (RV) is the leading cause of diarrhea-related death in children worldwide and 95% of RV-associated deaths occur in Africa and Asia where RV vaccines (RVVs) have lower efficacy. We hypothesize that differences in intestinal microbiome composition correlate with the decreased RVV efficacy observed in poor settings.

METHODS:  We conducted a nested, case-control study comparing prevaccination, fecal microbiome compositions between 6-week old, matched RVV responders and nonresponders in rural Ghana. These infants' microbiomes were then compared with 154 age-matched, healthy Dutch infants' microbiomes, assumed to be RVV responders. Fecal microbiome analysis was performed in all groups using the Human Intestinal Tract Chip.

RESULTS:  We analyzed findings in 78 Ghanaian infants, including 39 RVV responder and nonresponder pairs. The overall microbiome composition was significantly different between RVV responders and nonresponders (FDR, 0.12), and Ghanaian responders were more similar to Dutch infants than nonresponders (P = .002). RVV response correlated with an increased abundance of Streptococcus bovis and a decreased abundance of the Bacteroidetes phylum in comparisons between both Ghanaian RVV responders and nonresponders (FDR, 0.008 vs 0.003) and Dutch infants and Ghanaian nonresponders (FDR, 0.002 vs 0.009).

CONCLUSIONS:  The intestinal microbiome composition correlates significantly with RVV immunogenicity and may contribute to the diminished RVV immunogenicity observed in developing countries.

The gut microbiota as a modulator of innate immunity during melioidosis
Lankelma, Jacqueline M. ; Birnie, Emma ; Weehuizen, Tassili A.F. ; Scicluna, Brendon P. ; Belzer, Clara ; Houtkooper, Riekelt H. ; Roelofs, Joris J.T.H. ; Vos, Alex F. de; Poll, Tom van der; Budding, Andries E. ; Wiersinga, W.J. - \ 2017
PLoS Neglected Tropical Diseases 11 (2017)4. - ISSN 1935-2727
Background: Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is an emerging cause of pneumonia-derived sepsis in the tropics. The gut microbiota supports local mucosal immunity and is increasingly recognized as a protective mediator in host defenses against systemic infection. Here, we aimed to characterize the composition and function of the intestinal microbiota during experimental melioidosis. Methodology/Principal findings: C57BL/6 mice were infected intranasally with B. pseudomallei and sacrificed at different time points to assess bacterial loads and inflammation. In selected experiments, the gut microbiota was disrupted with broad-spectrum antibiotics prior to inoculation. Fecal bacterial composition was analyzed by means of IS-pro, a 16S-23S interspacer region-based profiling method. A marked shift in fecal bacterial composition was seen in all mice during systemic B. pseudomallei infection with a strong increase in Proteobacteria and decrease in Actinobacteria, with an increase in bacterial diversity. We found enhanced early dissemination of B. pseudomallei and systemic inflammation during experimental melioidosis in microbiota-disrupted mice compared with controls. Whole-genome transcriptional profiling of the lung identified several genes that were differentially expressed between mice with a normal or disrupted intestinal microbiota. Genes involved in acute phase signaling, including macrophage-related signaling pathways were significantly elevated in microbiota disrupted mice. Compared with controls, alveolar macrophages derived from antibiotic pretreated mice showed a diminished capacity to phagocytose B. pseudomallei. This might in part explain the observed protective effect of the gut microbiota in the host defense against pneumonia-derived melioidosis. Conclusions/Significance: Taken together, these data identify the gut microbiota as a potential modulator of innate immunity during B. pseudomallei infection.
Critically ill patients demonstrate large interpersonal variation in intestinal microbiota dysregulation : a pilot study
Lankelma, Jacqueline M. ; Vught, Lonneke A. van; Belzer, Clara ; Schultz, Marcus J. ; Poll, Tom van der; Vos, Willem M. de; Wiersinga, W.J. - \ 2017
Intensive Care Medicine 43 (2017)1. - ISSN 0342-4642 - p. 59 - 68.
Antibiotics - Critically ill - Gut microbiota - Intensive care unit - Sepsis

Purpose: The intestinal microbiota has emerged as a virtual organ with essential functions in human physiology. Antibiotic-induced disruption of the microbiota in critically ill patients may have a negative influence on key energy resources and immunity. We set out to characterize the fecal microbiota composition in critically ill patients both with and without sepsis and to explore the use of microbiota-derived markers for clinical outcome measurements in this setting. Methods: In this prospective observational cohort study we analyzed the fecal microbiota of 34 patients admitted to the intensive care unit. Fifteen healthy subjects served as controls. The fecal microbiota was phylogenetically characterized by 16S rRNA gene sequencing, and associations with clinical outcome parameters were evaluated. Results: A marked shift in fecal bacterial composition was seen in all septic and non-septic critically ill patients compared with controls, with extreme interindividual differences. In 13 of the 34 patients, a single bacterial genus made up >50% of the gut microbiota; in 4 patients this was even >75%. A significant decrease in bacterial diversity was observed in half of the patients. No associations were found between microbiota diversity, Firmicutes/Bacteroidetes ratio, or Gram-positive/Gram-negative ratio and outcome measurements such as complications and survival. Conclusions: We observed highly heterogeneous patterns of intestinal microbiota in both septic and non-septic critically ill patients. Nevertheless, some general patterns were observed, including disappearance of bacterial genera with important functions in host metabolism. More detailed knowledge of the short- and long-term health consequences of these major shifts in intestinal bacterial communities is needed.

Antibiotic-Induced Gut Microbiota Disruption Decreases TNF-α Release by Mononuclear Cells in Healthy Adults
Lankelma, Jacqueline M. ; Belzer, Clara ; Hoogendijk, Arie J. ; Vos, Alex F. de; Vos, Willem M. de; Poll, Tom van der; Wiersinga, W.J. - \ 2016
Clinical and Translational Gastroenterology 7 (2016)8. - ISSN 2155-384X

Objectives:Broad-spectrum antibiotics disrupt the intestinal microbiota. The microbiota is essential for physiological processes, such as the development of the gut immune system. Recent murine data suggest that the intestinal microbiota also modulates systemic innate immune responses; however, evidence in humans is lacking.Methods:Twelve healthy young men were given oral broad-spectrum antibiotics (ciprofloxacin 500 mg bid, vancomycin 500 mg tid and metronidazole 500 mg tid) for 7 days. At baseline, 1 day, and 6 weeks after antibiotics, blood and feces were sampled. Whole blood and isolated mononuclear cells were stimulated with selected Toll-like receptor agonists and heat-killed bacteria. Microbiota diversity and composition was determined using bacterial 16S rDNA sequencing.Results:One day after the antibiotic course, microbial diversity was significantly lower compared with baseline. After antibiotic therapy, systemic mononuclear cells produced lower levels of tumor necrosis factor (TNF)-α after ex vivo stimulation with lipopolysaccharide (LPS). This diminished capacity to produce TNF-α was restored 6 weeks after cessation of antibiotic therapy. In whole blood, a reduced capacity to release interleukin (IL)-1β and IL-6 was observed after LPS stimulation. Antibiotic treatment did not impact on differential leukocyte counts, phagocytosis, and cell surface markers of neutrophils and monocytes.Conclusions:In this proof-of-principle study of healthy subjects, microbiota disruption by broad-spectrum antibiotics is reversibly associated with decreased systemic cellular responsiveness towards LPS. The implications of these findings in a clinical setting remain to be determined.

Antibiotic-induced gut microbiota disruption during human endotoxemia: a randomised controlled study
Lankelma, Jacqueline M. ; Cranendonk, Duncan R. ; Belzer, Clara ; Vos, Alex F. De; Vos, Willem M. De; Poll, Tom Van Der; Wiersinga, W.J. - \ 2016
Gut 66 (2016)9. - ISSN 0017-5749 - p. 1623 - 1630.
Objective The gut microbiota is essential for the development of the intestinal immune system. Animal models have suggested that the gut microbiota also acts as a major modulator of systemic innate immunity during sepsis. Microbiota disruption by broad-spectrum antibiotics could thus have adverse effects on cellular responsiveness towards invading pathogens. As such, the use of antibiotics may attribute to immunosuppression as seen in sepsis. We aimed to test whether disruption of the gut microbiota affects systemic innate immune responses during endotoxemia in healthy subjects. Design In this proof-of-principle intervention trial, 16 healthy young men received either no treatment or broad-spectrum antibiotics (ciprofloxacin, vancomycin and metronidazole) for 7 days, after which all were administered lipopolysaccharide intravenously to induce a transient sepsis-like syndrome. At various time points, blood and faeces were sampled. Results Gut microbiota diversity was significantly lowered by the antibiotic treatment in all subjects. Clinical parameters, neutrophil influx, cytokine production, coagulation activation and endothelial activation during endotoxemia were not different between antibiotic-pretreated and control individuals. Antibiotic treatment had no impact on blood leucocyte responsiveness to various Toll-like receptor ligands and clinically relevant causative agents of sepsis (Streptococcus pneumoniae, Klebsiella pneumoniae, Escherichia coli) during endotoxemia. Conclusions These findings suggest that gut microbiota disruption by broad-spectrum antibiotics does not affect systemic innate immune responses in healthy subjects during endotoxemia in humans, disproving our hypothesis. Further research is needed to test this hypothesis in critically ill patients. These data underline the importance of translating findings in mice to humans.
The gut microbiota plays a protective role in the host defence against pneumococcal pneumonia
Schuijt, T.J. ; Lankelma, J.M. ; Scicluna, B.P. ; Melo, F.S. e; Roelofs, J.J. ; Boer, J.D. de; Hoogendijk, A.J. ; Beer, R. de; Vos, A. de; Belzer, C. ; Vos, W.M. de; Poll, T. van der; Wiersinga, W.J. - \ 2016
Gut 65 (2016). - ISSN 0017-5749 - p. 575 - 583.
OBJECTIVE: Pneumonia accounts for more deaths than any other infectious disease worldwide. The intestinal microbiota supports local mucosal immunity and is increasingly recognised as an important modulator of the systemic immune system. The precise role of the gut microbiota in bacterial pneumonia, however, is unknown. Here, we investigate the function of the gut microbiota in the host defence against Streptococcus pneumoniae infections. DESIGN: We depleted the gut microbiota in C57BL/6 mice and subsequently infected them intranasally with S. pneumoniae. We then performed survival and faecal microbiota transplantation (FMT) experiments and measured parameters of inflammation and alveolar macrophage whole-genome responses. RESULTS: We found that the gut microbiota protects the host during pneumococcal pneumonia, as reflected by increased bacterial dissemination, inflammation, organ damage and mortality in microbiota-depleted mice compared with controls. FMT in gut microbiota-depleted mice led to a normalisation of pulmonary bacterial counts and tumour necrosis factor-a and interleukin-10 levels 6 h after pneumococcal infection. Whole-genome mapping of alveolar macrophages showed upregulation of metabolic pathways in the absence of a healthy gut microbiota. This upregulation correlated with an altered cellular responsiveness, reflected by a reduced responsiveness to lipopolysaccharide and lipoteichoic acid. Compared with controls, alveolar macrophages derived from gut microbiota-depleted mice showed a diminished capacity to phagocytose S. pneumoniae. CONCLUSIONS: This study identifies the intestinal microbiota as a protective mediator during pneumococcal pneumonia. The gut microbiota enhances primary alveolar macrophage function. Novel therapeutic strategies could exploit the gut-lung axis in bacterial infections
The gut microbiota in internal medicine: implications for health and disease
Lankelma, J.M. ; Nieuwdorp, M. ; Vos, W.M. de; Wiersinga, W.J. - \ 2015
The Netherlands Journal of Medicine 73 (2015)2. - ISSN 0300-2977 - p. 61 - 68.
The human gut microbiota may be viewed as an organ, executing numerous functions in metabolism, development of the immune system and host defence against pathogens. It may therefore be involved in the development of a range of diseases such as gastrointestinal infections, inflammatory bowel disease, allergy and diabetes mellitus. Reversely, certain therapies that are often used, such as antibiotics and chemotherapy, may negatively affect the composition and function of the gut microbiota and thereby the wellbeing of patients. As the microbiota research field is currently moving from association studies to intervention studies and even clinical trials, implementation of this new knowledge into clinical practice is coming near. Several therapeutic interventions that target the gut microbiota are being evaluated, ranging from supplementation of food components to transplantation of faecal microbiota. In this review we provide an overview of current literature on the gut microbiota in both a healthy state and a range of diseases that are relevant for internal medicine. In anticipation of gut microbiota-targeted therapies, it is important to realise the key function of the gut microbiota in physiological processes and the collateral damage that may be caused when disrupting this ecosystem within us
Grote investering in natuurherstel vereist onderzoek. Kennisontwikkeling is van doorslaggevend belang voor het slagen van de PAS
Bobbink, R. ; Jansen, A. ; Siepel, H. ; Verstrael, T. ; Wiersinga, W. - \ 2015
Vakblad Natuur Bos Landschap 12 (2015)113. - ISSN 1572-7610 - p. 3 - 5.
natuurbeheer - herstel - stikstof - herstelbeheer - wetenschappelijk onderzoek - kennismanagement - provincies - nature management - rehabilitation - nitrogen - restoration management - scientific research - knowledge management - provinces
De Programmatische Aanpak Stikstof (PAS) moet in de komende achttien jaar zorgen dat stikstofgevoelige natuur weer opfleurt in Natura 2000-gebieden: er wordt gestreefd naar een combinatie van economische ontwikkeling, dalende stikstofdepositie en natuurherstel. Daarvoor worden in de komende tijd flinke investeringen gedaan. Om de kans op succes te vergroten, en de altijd aanwezige risico’s zo klein mogelijk te houden, is begeleidend wetenschappelijk onderzoek van groot belang. Het Kennisnetwerk OBN kan een grote bijdrage leveren aan de opzet, begeleiding en toetsing van toekomstig onderzoek zodat de PAS-herstelmaatregelen echt effectief zijn.
De darmmicrobiota in gezondheid en ziekte
Lankelma, J.M. ; Nieuwdorp, M. ; Vos, W.M. de; Wiersinga, W.J. - \ 2014
Nederlands Tijdschrift voor Geneeskunde 157 (2014). - ISSN 0028-2162 - 8 p.
De darmmicrobiota, voorheen ‘darmflora’ genoemd, kunnen worden beschouwd als een extern orgaan dat vele fysiologische functies vervult in het metabolisme, de ontwikkeling van het immuunsysteem en de afweer tegen pathogenen. De volwassen darmmicrobiota bestaan uit 1013-1014 micro-organismen. Het gezamenlijke genoom daarvan, ook wel het microbioom genoemd, is 100 keer groter dan het menselijke genoom. De darmmicrobiota spelen mogelijk een rol bij de pathogenese van een scala aan ziektebeelden, zoals inflammatoire darmziekten, obesitas, diabetes mellitus en atopische aandoeningen. Een kanttekening hierbij is dat tot dusver voornamelijk associatiestudies uitgevoerd zijn, zonder bewijs van causaliteit. Dit toenemende inzicht heeft geleid tot de identificatie van nieuwe therapeutische strategieën, die op dit moment in klinische studies getest worden. Hoewel nog moet blijken wat deze kennis voor de individuele patiënt betekent, zijn verschillende interventies denkbaar, zoals suppletie van voedingsbestanddelen, prebiotica, probiotica en fecestransplantatie.
Sustainable vegetable chain development in Brazil: Grape tomatoes from Holambra to the higher market segment
Wiersinga, R.C. ; Vermeulen, T. ; Snels, J.C.M.A. ; Fray da Silva, R. ; Wiersema, S.G. ; Mei, M. van der - \ 2013
Wageningen : Wageningen UR Glastuinbouw (Rapport / Wageningen UR Glastuinbouw 1262) - 33
Holambra (Brazilie) is een groot tuinbouwcluster nabij Sao Paolo. Het cluster is traditioneel op sierteelt gericht. Een aantal telers zijn gaan experimenteren met bedekte groenteteelt om zo het gebruik van gewasbeschermingsmiddelen te kunnen reduceren en voor een hoger marktsegment te kunnen telen. Wageningen UR kon deze ondernemers helpen bij het verbeteren van hun teeltsystemen door een systematische check op het wortelmilieu, de voedingshuishouding en het teeltklimaat. Daarbij zijn de knelpunten in de supply-chain in kaart gebracht. Op basis van deze analyse konden ondernemers keuzes maken voor investeringen in gerichte verbetering van deze supply chain. Het project is vervolgens aanjager geweest in het gebied voor verdere samenwerking tussen tuinders op R&D en kwaliteitsmanagement. Holambra (Brasil) has a large horticultural cluster for flowers and ornamentals. However, only few growers use protected cultivation for vegetable productions. This study analysed the market and market chain relationships for vegetables in the Sao Paolo region as well as the technology used for cultivation. With the rising middle class the demand for quality vegetables with high food safety standards is rising strongly. The growers supplying this market niche are aware of these quality demands and are able to deliver according to them. The growers had been developing their production systems individually, but were aware of the advantage in closer cooperation on R&D as well as improving supply chain quality (cooled chains). The technological developments were needed in areas of climate control and water and nutrition steering. During the project growers were given tools to improve their systems as well as identify the crucial factors for quality control in the supply chain. The study was conducted by LEI and Wageningen UR Greenhouse Horticulture
The intestinal microbiota and host immune interactions in the critically ill
Schuijt, T.J. ; Poll, T. van der; Vos, W.M. de; Wiersinga, W.J. - \ 2013
Trends in Microbiology 21 (2013)5. - ISSN 0966-842X - p. 221 - 229.
ventilator-associated pneumonia - systemic innate immunity - human gut microbiome - toll-like receptors - chain fatty-acids - selective decontamination - critical illness - intensive-care - clostridium-difficile - commensal microbiota
The gastrointestinal tract harbors a complex population of microbes that play a fundamental role in the development of the immune system and human health. Besides an important local contribution in the host defense against infections, it has become increasingly clear that intestinal bacteria also modulate immune responses at systemic sites. These new insights can be of profound clinical relevance especially for intensive care medicine where the majority of patients are treated with antibiotics, which have pervasive and long-term effects on the intestinal microbiota. Moreover, considerable progress has been made in defining the role of the intestinal microbiota in both health and disease. In this review, we highlight these aspects and focus on recent key findings addressing the role of intestinal microbiota in antimicrobial defense mechanisms and its impact on intestinal homeostasis in the critically ill
Natuurverkenning 2010-2040. Achtergrondrapport
Dammers, E. ; Hinsberg, A. van; Wiersinga, W. ; Egmond, P. van; Vader, J. ; Melman, T.C.P. ; Bilt, W. van der; Oostenbrugge, R. van - \ 2013
Den Haag : Planbureau voor de Leefomgeving (PBL-publicatienummer 500414010) - ISBN 9789491506291 - 190
natuurbeheer - natuurbeleid - toekomst - scenario-analyse - landschapsbeheer - nature management - nature conservation policy - future - scenario analysis - landscape management
Het Planbureau voor de Leefomgeving (PBL) brengt elke vier jaar een Natuurverkenning uit. Dit is één van de wettelijke taken van het planbureau. Een toekomstverkenning is bedoeld om toekomstige maatschappelijke ontwikkelingen en nieuwe beleidsopties te verkennen en daarmee het blikveld te verruimen. Deze Natuurverkenning zoekt aansluiting bij de huidige politieke en maatschappelijke discussie over de richting van het natuur- en landschapsbeleid. Het is de vierde Natuurverkenning die het PBL en zijn voorlopers hebben uitgebracht. De eerste en de tweede (RIVM 1997; 2002) zijn als zelfstandige studies verschenen en de derde als onderdeel van Nederland later (MNP 2006)
Towards consumer driven and innovative fruit supply chains
Wiersinga, R.C. ; Hiller, S.R.C.H. ; Zimmerman, K.L. - \ 2012
Acta Horticulturae 930 (2012). - ISSN 0567-7572 - p. 209 - 216.
This paper aims to identify supply chain organization and management structures that maximize innovation in consumer driven fruit supply chains with the ultimate goal of increasing European fruit consumption. Data was collected on the chain organization, management structures and innovativeness of ten innovative fruit supply chains in Poland, Spain, Greece and The Netherlands using two questionnaires for each chain actor. The supply chains have been analysed based on a conceptual framework of mechanisms underlying supply chain management. After comparing the selected fruit supply chains against the conceptual framework, critical success factors (CSFs) were derived for fruit supply chain innovativeness. For every type of fruit chain, it is important to have a customer focus, a quality management system implementing tracking and tracing, and a lead firm in the chain that initiates, stimulates and coordinates the implementation of innovations. A typology of consumer driven innovative fruit supply chains is proposed based on the different combinations of network use and formality of the innovation strategy in the chains. The choice of type for a chain depends on the entrepreneurial style, type of product (fresh or processed) and size of the companies. The guidelines support the identification of CSFs.
Making scenarios for nature policy in the Netherlands
Dammers, E. ; Hinsberg, A. van; Vader, J. ; Wiersinga, W.A. - \ 2012
In: Proceedings 26th AESOP Conference 2012. - - p. 5430 - 5444.
The subsequent building blocks of the scenario study enable policymakers to gradually gain insight into alternative desirable future states of nature and alternative policy-strategies to realize them. This is further stimulated by the character of the scenarios, which contain not only qualitative storylines, but also concrete images and quantita-tive information. In order to build such scenarios various methods were applied, like model calculations, scenario workshops, and design activities. By participating in the scenario study, policymakers not only provided valuable insights but also became more receptive for the results.
Wie besturen de Wadden en wat kan beter?
Wiersinga, W.A. - \ 2012
Vakblad Natuur Bos Landschap 9 (2012)8. - ISSN 1572-7610 - p. 4 - 7.
natuurgebieden - wadden - natuurbeleid - provincies - natural areas - tidal flats - nature conservation policy - provinces
Te veel organisaties bemoeien zich met het besturen en beheren van het Waddengebied. Maar waardoor wordt deze bestuurlijke drukte veroorzaakt, hoe ziet zij er uit en is er een oplossing voor dit probleem?
Ik ben wel eens bij zo'n circus geweest
Klostermann, J.E.M. ; Wiersinga, W. ; Schuiling, E. - \ 2012
Wageningen : Alterra (Alterra-rapport 2357) - 82
nederlandse waddeneilanden - hoogwaterbeheersing - veiligheid - zoet water - watervoorziening - governance - participatie - burgers - dutch wadden islands - flood control - safety - fresh water - water supply - governance - participation - citizens
Dit onderzoek analyseert voor het Deltaprogramma Waddengebied de belangen en visies van diverse lokale stakeholders op veiligheid op de eilanden en de vastelandskust. Hoewel langs de vastelandskust veiligheidsproblemen moeten worden opgelost is daar weinig maatschappelijke discussie over; er zijn veel mogelijke oplossingen zoals ruimtelijke ordeningsmaatregelen, multifunctionele dijken en aanleg/behoud van kwelders. Op Texel en Vlieland spelen potentieel heftige veiligheidsdiscussies. Op Terschelling, Ameland en Schiermonnikoog is geen actueel veiligheidsissue aan de orde maar daar zijn wel issues van cultuurhistorie en vrijheid versus natuurlijke dynamiek en meegroeien met de zee om de veiligheid op de lange termijn te waarborgen. Buitendijkse gebieden met problemen zijn vooral havens, kaden, pieren en veerdammen, die nu bij hoog water al in de problemen komen. Er is echter weinig discussie over en er zijn nog geen oplossingen in beeld. De zoetwatervoorziening voor de landbouw op de vastelandskust is afhankelijk van de watervoorraad in het IJsselmeer. De drinkwatervoorziening op de eilanden is nu al een lastige taak voor de drinkwaterbedrijven. Nieuwe ideeën om aan de drinkwatervraag te voldoen zijn o.a. het zoete water gaan gebruiken dat het waterschap uitslaat naar zee, en met techniek de watervraag naar beneden brengen. Het vervolg van de participatiestrategie voor het Deltaprogramma Wadden kan uit de volgende onderdelen bestaan: Expertise betrekken, netwerk uitbouwen en transparantie en openheid bieden.
Zand erover : vier scenario's voor zachte kustverdediging
Baptist, M.J. ; Wiersinga, W.A. - \ 2012
De Levende Natuur 113 (2012)2. - ISSN 0024-1520 - p. 56 - 61.
kustgebieden - bescherming - kustbeheer - natuurbeheer - zandsuppletie - coastal areas - protection - coastal management - nature management - sand suppletion
Klimaatverandering en zeespiegelstijging houden het thema kustveiligheid actueel. Regelmatig worden kustsuppleties uitgevoerd, maar de vraag rijst of met de voorspelde zeespiegelstijging veel meer zand op de kust gesuppleerd zou moeten worden. En wat zullen de effecten daarvan zijn? Dit hangt met name samen met de wijze en frequentie van suppleren. Voor de Natuurverkenning 2011 zijn daarom vier scenario’s voor de kustverdediging ontwikkeld. Voor ieder scenario is op basis van expert judgement uitgewerkt hoe de natuur van de kustzee er in 2040 uit kan zien. Met een model voor de omvang van de suppleties, de herhaalperiode van de suppleties en de hersteltijden van de kustzee is aangegeven welke aanpak ecologisch duurzaam is.
Natuurverkenning 2010-2040 : visies op de ontwikkeling van natuur en landschap
Oostenbrugge, R. van; Egmond, P. van; Dammers, E. ; Hinsberg, A. van; Melman, T.C.P. ; Vader, J. ; Wiersinga, W.A. ; Bilt, W. van der; Bredenoord, H. ; Gaalen, F. van; Nijhuis, L. ; Puijenbroek, P. van; Tekelenburg, T. ; Wortelboer, R. ; Dirkx, G.H.P. ; Groenendijk, F.C. ; Jorritsma, I.T.M. ; Knegt, B. de; Sijtsma, F. ; Dijkman, W. - \ 2012
Den Haag : Planbureau voor de Leefomgeving - ISBN 9789078645887 - 138
natuur - landschap - natuurbeleid - landschapsplanning - overheidsbeleid - toekomst - scenario-analyse - nature - landscape - nature conservation policy - landscape planning - government policy - future - scenario analysis
De Natuurverkenning verschijnt in een turbulente tijd waarin natuur en landschap sterk gepolitiseerd zijn. Met de verkenning wil het PBL een bijdrage leveren aan het structureren van het debat over de vernieuwing van het langetermijnbeleid en een impuls geven aan de politieke afwegingen. Nieuw is het gebruik van normatieve toekomstscenario’s als hulpmiddel om de achterliggende drijfveren voor natuurbeleid te verhelderen.
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