Maternal allergy and the presence of nonhuman proteinaceous molecules in human milk
Dekker, Pieter M. ; Boeren, Sjef ; Wijga, Alet H. ; Koppelman, Gerard H. ; Vervoort, Jacques J.M. ; Hettinga, Kasper A. - \ 2020
Nutrients 12 (2020)4. - ISSN 2072-6643
Allergen transfer - Human milk - Maternal allergy - Nonhuman proteins - β-lactoglobulin
Human milk contains proteins and/or protein fragments that originate from nonhuman organisms. These proteinaceous molecules, of which the secretion might be related to the mother’s allergy status, could be involved in the development of the immune system of the infant. This may lead, for example, to sensitization or the induction of allergen-specific tolerance. The aim of this study was to investigate the relation between maternal allergy and the levels of nonhuman proteinaceous molecules in their milk. In this study, we analysed trypsin-digested human milk serum proteins of 10 allergic mothers and 10 nonallergic mothers. A search was carried out to identify peptide sequences originating from bovine or other allergenic proteins. Several methods were applied to confirm the identification of these sequences, and the differences between both groups were investigated. Out of the 78 identified nonhuman peptide sequences, 62 sequences matched Bos taurus proteins. Eight peptide sequences of bovine β-lactoglobulin had significantly higher levels in milk from allergic mothers than in milk from nonallergic mothers. Dietary bovine β-lactoglobulin may be absorbed through the intestinal barrier and secreted into human milk. This seems to be significantly higher in allergic mothers and might have consequences for the development of the immune system of their breastfed infant.
Impact of maternal body mass index and gestational weight gain on pregnancy complications: an individual participant data meta-analysis of European, North American and Australian cohorts
Santos, S. ; Voerman, E. ; Amiano, P. ; Barros, H. ; Beilin, L.J. ; Bergström, A. ; Charles, M.A. ; Chatzi, L. ; Chevrier, C. ; Chrousos, G.P. ; Corpeleijn, E. ; Costa, O. ; Costet, N. ; Crozier, S. ; Devereux, G. ; Doyon, M. ; Eggesbø, M. ; Fantini, M.P. ; Farchi, S. ; Forastiere, F. ; Georgiu, V. ; Godfrey, K.M. ; Gori, D. ; Grote, V. ; Hanke, W. ; Hertz-Picciotto, I. ; Heude, B. ; Hivert, M.F. ; Hryhorczuk, D. ; Huang, R.C. ; Inskip, H. ; Karvonen, A.M. ; Kenny, L.C. ; Koletzko, B. ; Küpers, L.K. ; Lagström, H. ; Lehmann, I. ; Magnus, P. ; Majewska, R. ; Mäkelä, J. ; Manios, Y. ; McAuliffe, F.M. ; McDonald, S.W. ; Mehegan, J. ; Melén, E. ; Mommers, M. ; Morgen, C.S. ; Moschonis, G. ; Murray, D. ; Ní Chaoimh, C. ; Nohr, E.A. ; Nybo Andersen, A.M. ; Oken, E. ; Oostvogels, A.J.J.M. ; Pac, A. ; Papadopoulou, E. ; Pekkanen, J. ; Pizzi, C. ; Polanska, K. ; Porta, D. ; Richiardi, L. ; Rifas-Shiman, S.L. ; Roeleveld, N. ; Ronfani, L. ; Santos, A.C. ; Standl, M. ; Stigum, H. ; Stoltenberg, C. ; Thiering, E. ; Thijs, C. ; Torrent, M. ; Tough, S.C. ; Trnovec, T. ; Turner, S. ; Gelder, M.M.H.J. van; Rossem, L. van; Berg, A. von; Vrijheid, M. ; Vrijkotte, T.G.M. ; West, J. ; Wijga, A.H. ; Wright, J. ; Zvinchuk, O. ; Sørensen, T.I.A. ; Lawlor, D.A. ; Gaillard, R. ; Jaddoe, V.W.V. - \ 2019
BJOG : an international journal of obstetrics and gynaecology 126 (2019)8. - ISSN 1470-0328 - p. 984 - 995.
Birthweight - body mass index - pregnancy complications - preterm birth - weight gain
Objective: To assess the separate and combined associations of maternal pre-pregnancy body mass index (BMI) and gestational weight gain with the risks of pregnancy complications and their population impact. Design: Individual participant data meta-analysis of 39 cohorts. Setting: Europe, North America, and Oceania. Population: 265 270 births. Methods: Information on maternal pre-pregnancy BMI, gestational weight gain, and pregnancy complications was obtained. Multilevel binary logistic regression models were used. Main outcome measures: Gestational hypertension, pre-eclampsia, gestational diabetes, preterm birth, small and large for gestational age at birth. Results: Higher maternal pre-pregnancy BMI and gestational weight gain were, across their full ranges, associated with higher risks of gestational hypertensive disorders, gestational diabetes, and large for gestational age at birth. Preterm birth risk was higher at lower and higher BMI and weight gain. Compared with normal weight mothers with medium gestational weight gain, obese mothers with high gestational weight gain had the highest risk of any pregnancy complication (odds ratio 2.51, 95% CI 2.31– 2.74). We estimated that 23.9% of any pregnancy complication was attributable to maternal overweight/obesity and 31.6% of large for gestational age infants was attributable to excessive gestational weight gain. Conclusions: Maternal pre-pregnancy BMI and gestational weight gain are, across their full ranges, associated with risks of pregnancy complications. Obese mothers with high gestational weight gain are at the highest risk of pregnancy complications. Promoting a healthy pre-pregnancy BMI and gestational weight gain may reduce the burden of pregnancy complications and ultimately the risk of maternal and neonatal morbidity. Tweetable abstract: Promoting a healthy body mass index and gestational weight gain might reduce the population burden of pregnancy complications.
Maternal body mass index, gestational weight gain, and the risk of overweight and obesity across childhood : An individual participant data meta-analysis
Voerman, Ellis ; Santos, Susana ; Patro Golab, Bernadeta ; Amiano, Pilar ; Ballester, Ferran ; Barros, Henrique ; Bergström, Anna ; Charles, Marie Aline ; Chatzi, Leda ; Chevrier, Cécile ; Chrousos, George P. ; Corpeleijn, Eva ; Costet, Nathalie ; Crozier, Sarah ; Devereux, Graham ; Eggesbø, Merete ; Ekström, Sandra ; Fantini, Maria Pia ; Farchi, Sara ; Forastiere, Francesco ; Georgiu, Vagelis ; Godfrey, Keith M. ; Gori, Davide ; Grote, Veit ; Hanke, Wojciech ; Hertz-Picciotto, Irva ; Heude, Barbara ; Hryhorczuk, Daniel ; Huang, Rae Chi ; Inskip, Hazel ; Iszatt, Nina ; Karvonen, Anne M. ; Kenny, Louise C. ; Koletzko, Berthold ; Küpers, Leanne K. ; Lagström, Hanna ; Lehmann, Irina ; Magnus, Per ; Majewska, Renata ; Mäkelä, Johanna ; Manios, Yannis ; McAuliffe, Fionnuala M. ; McDonald, Sheila W. ; Mehegan, John ; Mommers, Monique ; Morgen, Camilla S. ; Mori, Trevor A. ; Moschonis, George ; Murray, Deirdre ; Chaoimh, Carol Ní ; Nohr, Ellen A. ; Nybo Andersen, Anne Marie ; Oken, Emily ; Oostvogels, Adriëtte J.J.M. ; Pac, Agnieszka ; Papadopoulou, Eleni ; Pekkanen, Juha ; Pizzi, Costanza ; Polanska, Kinga ; Porta, Daniela ; Richiardi, Lorenzo ; Rifas-Shiman, Sheryl L. ; Ronfani, Luca ; Santos, Ana C. ; Standl, Marie ; Stoltenberg, Camilla ; Thiering, Elisabeth ; Thijs, Carel ; Torrent, Maties ; Tough, Suzanne C. ; Trnovec, Tomas ; Turner, Steve ; Rossem, Lenie van; Berg, Andrea von; Vrijheid, Martine ; Vrijkotte, Tanja G.M. ; West, Jane ; Wijga, Alet ; Wright, John ; Zvinchuk, Oleksandr ; Sørensen, Thorkild I.A. ; Lawlor, Debbie A. ; Gaillard, Romy ; Jaddoe, Vincent W.V. - \ 2019
PLOS Medicine 16 (2019)2. - ISSN 1549-1676 - p. e1002744 - e1002744.
BACKGROUND: Maternal obesity and excessive gestational weight gain may have persistent effects on offspring fat development. However, it remains unclear whether these effects differ by severity of obesity, and whether these effects are restricted to the extremes of maternal body mass index (BMI) and gestational weight gain. We aimed to assess the separate and combined associations of maternal BMI and gestational weight gain with the risk of overweight/obesity throughout childhood, and their population impact. METHODS AND FINDINGS: We conducted an individual participant data meta-analysis of data from 162,129 mothers and their children from 37 pregnancy and birth cohort studies from Europe, North America, and Australia. We assessed the individual and combined associations of maternal pre-pregnancy BMI and gestational weight gain, both in clinical categories and across their full ranges, with the risks of overweight/obesity in early (2.0-5.0 years), mid (5.0-10.0 years) and late childhood (10.0-18.0 years), using multilevel binary logistic regression models with a random intercept at cohort level adjusted for maternal sociodemographic and lifestyle-related characteristics. We observed that higher maternal pre-pregnancy BMI and gestational weight gain both in clinical categories and across their full ranges were associated with higher risks of childhood overweight/obesity, with the strongest effects in late childhood (odds ratios [ORs] for overweight/obesity in early, mid, and late childhood, respectively: OR 1.66 [95% CI: 1.56, 1.78], OR 1.91 [95% CI: 1.85, 1.98], and OR 2.28 [95% CI: 2.08, 2.50] for maternal overweight; OR 2.43 [95% CI: 2.24, 2.64], OR 3.12 [95% CI: 2.98, 3.27], and OR 4.47 [95% CI: 3.99, 5.23] for maternal obesity; and OR 1.39 [95% CI: 1.30, 1.49], OR 1.55 [95% CI: 1.49, 1.60], and OR 1.72 [95% CI: 1.56, 1.91] for excessive gestational weight gain). The proportions of childhood overweight/obesity prevalence attributable to maternal overweight, maternal obesity, and excessive gestational weight gain ranged from 10.2% to 21.6%. Relative to the effect of maternal BMI, excessive gestational weight gain only slightly increased the risk of childhood overweight/obesity within each clinical BMI category (p-values for interactions of maternal BMI with gestational weight gain: p = 0.038, p < 0.001, and p = 0.637 in early, mid, and late childhood, respectively). Limitations of this study include the self-report of maternal BMI and gestational weight gain for some of the cohorts, and the potential of residual confounding. Also, as this study only included participants from Europe, North America, and Australia, results need to be interpreted with caution with respect to other populations. CONCLUSIONS: In this study, higher maternal pre-pregnancy BMI and gestational weight gain were associated with an increased risk of childhood overweight/obesity, with the strongest effects at later ages. The additional effect of gestational weight gain in women who are overweight or obese before pregnancy is small. Given the large population impact, future intervention trials aiming to reduce the prevalence of childhood overweight and obesity should focus on maternal weight status before pregnancy, in addition to weight gain during pregnancy.
After genome-wide association studies : Gene networks elucidating candidate genes divergences for number of teats across two pig populations
Verardo, L.L. ; Lopes, M.S. ; Wijga, S. ; Madsen, O. ; Silva, F.F. ; Groenen, M.A.M. ; Knol, E.F. ; Lopes, P.S. ; Guimarães, S.E.F. - \ 2016
Journal of Animal Science 94 (2016)4. - ISSN 0021-8812 - p. 1446 - 1458.
Complex trait - Genetic diversity - Genomewide association study
Number of teats (NT) is an important trait affecting both piglet’s welfare and the production level of pig farms. Biologically, embryonic mammary gland development requires the coordination of many signaling pathways necessary for the proper development of teats. Several QTL for NT have been identified; however, further analysis is still lacking. Therefore, gene networks derived from genomewide association study (GWAS) results can be used to examine shared pathways and functions of putative candidate genes. Besides, such analyses may also be helpful to understand the genetic diversity between populations for the same trait or traits. In this study, we identified significant SNP for Landrace-based (line C) and Large White–based (line D) dam lines. Besides, gene– transcription factor (TF) networks were constructed aiming to obtain the most likely candidate genes for NT in each line followed by a comparative analysis between both lines to access similarities or dissimilarities at the marker and gene level. We identified 24 and 19 significant SNP (Bayes factor ≥ 100) for lines C and D, respectively. Only 1 significant SNP overlapped both lines. Network analysis illustrated gene interactions consistent with known mammal’s breast biology and captured known TF. We observed different sets of putative candidate genes for NT in each line evaluated that may have common effects on the phenotype. Based on these results, we demonstrated the importance of post-GWAS analyses increasing the biological understanding of relevant genes for a complex trait. Moreover, we believe that this genomic diversity across lines should be taken into account, considering breed-specific reference populations for genomic selection.
Difference in the Breast Milk Proteome between Allergic and Non-Allergic Mothers
Hettinga, K.A. ; Reina, F.M. ; Boeren, J.A. ; Zhang, L. ; Koppelman, G.H. ; Postma, D.S. ; Vervoort, J.J.M. ; Wijga, A.H. - \ 2015
PLoS ONE 10 (2015)3. - ISSN 1932-6203 - 11 p.
Background Breastfeeding has been linked to a reduction in the prevalence of allergy and asthma. However, studies on this relationship vary in outcome, which may partly be related to differences in breast milk composition. In particular breast milk composition may differ between allergic and non-allergic mothers. Important components that may be involved are breast milk proteins, as these are known to regulate immune development in the newborn. The objective of this study was therefore to explore differences in the proteins of breast milk from 20 allergic and non-allergic mothers. The results from this comparison may then be used to generate hypotheses on proteins associated with allergy in their offspring. Methods Milk samples from allergic and non-allergic mothers were obtained from the PIAMA project, a prospective birth cohort study on incidence, risk factors, and prevention of asthma and inhalant allergy. Non-targeted proteomics technology, based on liquid chromatography and mass spectrometry, was used to compare breast milk from allergic and non-allergic mothers. Results Nineteen proteins, out of a total of 364 proteins identified in both groups, differed significantly in concentration between the breast milk of allergic and non-allergic mothers. Protease inhibitors and apolipoproteins were present in much higher concentrations in breast milk of allergic than non-allergic mothers. These proteins have been suggested to be linked to allergy and asthma. Conclusions The non-targeted milk proteomic analysis employed has provided new targets for future studies on the relation between breast milk composition and allergy.
Plasma-Serum Cholesterol Differences in Children and Use of Measurements from Different Specimens
Berentzen, N.E. ; Wijga, A.H. ; Rossem, L. van; Jongste, J.C. de; Boshuizen, H.C. ; Smit, H.A. - \ 2013
Annals of Nutrition & Metabolism 63 (2013)4. - ISSN 0250-6807 - p. 305 - 310.
density-lipoprotein cholesterol - lipid-levels - risk - hypercholesterolemia - apolipoproteins - standardization - triglycerides
Background: We aimed to assess absolute plasma-serum differences and differences in ranking of total cholesterol (TC), high-density lipoprotein cholesterol (HDLC), and TC/HDLC ratio in children. Methods: We analysed data of 412 children participating in a Dutch birth cohort. TC, HDLC, and TC/HDLC ratio were determined in plasma at age 8 and 12 years and in serum at age 12 years. Results: Compared to serum, plasma TC at age 12 years was 0.07 mmol/l lower (95% CI -0.08 to -0.06), plasma HDLC was 0.06 mmol/l higher (95% CI 0.05-0.07), and plasma TC/HDLC ratio was 0.19 lower (95% CI -0.20 to -0.17) (p <0.0001). Intraclass correlation coefficients (ICCs) for ranking of TC, HDLC, and TC/HDLC ratio at age 12 years were 0.970, 0.745, and 0.979, respectively. ICCs for ranking of 8- to 12-year change of TC, HDLC, and TC/HDLC ratio were 0.971, 0.957, and 0.955, respectively. Conclusions: Cholesterol was systematically different in plasma and serum, and use of plasma would result in a more favourable lipid profile of children (lower TC, higher HDLC, and lower TC/HDLC ratio). Nevertheless, consistency in ranking of children according to plasma or serum cholesterol concentrations was very high. Age-related change in cholesterol can be validly assessed by ranking the difference between serum concentrations at one age and plasma concentrations at another age. (C) 2014 S. Karger AG, Basel
|Genetic variation in macro- and micro-environmental sensitivity for milk yield in Swedish Holsteins
Mulder, H.A. ; Ronnegard, L. ; Wijga, S. ; Fikse, W.F. ; Veerkamp, R.F. ; Strandberg, E. - \ 2013
In: Book of Abstracts of the 64th Annual Meeting of the European Federation of Animal Science, 26-30 August 2013, Nantes, France. - Wageningen : Wageningen Academic Publishers - ISBN 9789086867820 - p. 215 - 215.
Genetic parameters for natural antibody isotype titers in milk of Dutch Holstein-Friesians
Wijga, S. ; Bovenhuis, H. ; Bastiaansen, J.W.M. ; Arendonk, J.A.M. van; Ploegaert, T.C.W. ; Tijhaar, E. ; Poel, J.J. van der - \ 2013
Animal Genetics 44 (2013)5. - ISSN 0268-9146 - p. 485 - 492.
immunoglobulin-a - innate immunity - laying hens - responses - mastitis - cattle - cows - lactation - survival
The objective of the present study was to estimate genetic parameters for natural antibody isotypes immunoglobulin (Ig) A, IgG1 and IgM titers binding the bacterial antigens lipopolysaccharide, peptidoglycan and the model antigen keyhole limpet hemocyanin in Dutch Holstein-Friesian cows (n = 1695). Further, this study included total natural antibody titers binding the antigens mentioned above, making no isotype distinction, as well as total natural antibody titers and natural antibody isotypes IgA, IgG1 and IgM binding lipoteichoic acid. The study showed that natural antibody isotype titers are heritable, ranging from 0.06 to 0.55, and that these heritabilities were generally higher than heritabilities for total natural antibody titers. Genetic correlations, the combinations of total natural antibody titers and natural antibody isotype titers, were nearly all positive and ranged from -0.23 to 0.99. Strong genetic correlations were found between IgA and IgM. Genetic correlations were substantially weaker when they involved an IgG1 titer, indicating that IgA and IgM have a common genetic basis, but that the genetic basis for IgG1 differs from that for IgA or IgM. Results from this study indicate that natural antibody isotype titers show the potential for effective genetic selection. Further, natural antibody isotypes may provide a better characterization of different elements of the immune response or immune competence. As such, natural antibody isotypes may enable more effective decisions when breeding programs start to include innate immune parameters.
The association between indoor temperature and body mass index in children: the PIAMA birth cohort study
Scheffers, F.R. ; Bekkers, M.B.M. ; Kerhof, M. ; Gehring, U. ; Koppelman, G.H. ; Schipper, M. ; Haveman-Nies, A. ; Wijga, A.H. - \ 2013
BMC Public Health 13 (2013). - ISSN 1471-2458 - 10 p.
mild cold - ambient-temperature - energy-expenditure - metabolism - weight - thermogenesis - prevalence - responses - obesity - humans
Background Several experimental studies showed consistent evidence for decreased energy expenditure at higher ambient temperatures. Based on this, an association between thermal exposure and body weight may be expected. However, the effect of thermal exposure on body weight has hardly been studied. Therefore, this study investigated the association between indoor temperature and body mass index (BMI) in children in real life. Methods This longitudinal observational study included 3 963 children from the Dutch Prevention and Incidence of Asthma and Mite Allergy (PIAMA) birth cohort that started in 1996. These children were followed from birth until the age of 11 years. Winter indoor temperature (living room and bedroom) was reported at baseline and BMI z-scores were available at 10 consecutive ages. Missing data were multiply imputed. Associations between indoor temperature and BMI were analyzed using generalized estimating equations (GEE), adjusted for confounders and stratified by gender. In a subgroup of 104 children, bedroom temperature was also measured with data loggers. Results Mean reported living room and bedroom temperature were 20.3°C and 17.4°C, respectively. Reported and measured bedroom temperatures were positively correlated (r¿=¿0.42, p¿=¿0.001). Neither reported living room temperature (-0.03¿=¿ß¿=¿0.04) and bedroom temperature (-0.01¿=¿ß¿=¿0.02) nor measured bedroom temperature (-0.04¿=¿ß¿=¿0.05) were associated with BMI z-score between the age of 3 months and 11 years. Conclusions This study in children did not support the hypothesized association between indoor temperature and BMI in a real life setting.
Immunogenetics in dairy cattle : somatic cell count and natural antibody levels
Wijga, S. - \ 2013
Wageningen University. Promotor(en): Johan van Arendonk, co-promotor(en): Henk Bovenhuis; John Bastiaansen. - S.l. : s.n. - ISBN 9789461737274 - 178
melkvee - melkkoeien - immunogenetica - celgetal - natuurlijke antilichamen - genomica - immunologie - genetica - dairy cattle - dairy cows - immunogenetics - somatic cell count - natural antibodies - genomics - immunology - genetics
There remains is a lot to be learned about the interpretation of genetic parameters and the biology of disease resistance and SCS. This PhD thesis aimed to obtain additional insight in disease resistance and SCS by: 1) quantifying the impact of genetics on innate immunity, represented by natural antibodies (NAb), through estimation of heritabilities and genetic correlations; 2) identifying the genomic regions involved in SCS and NAb levels; 3) quantifying the impact of genetics on environmental sensitivity for SCS.
Natural antibody levels are heritable with heritabilities ranging from 0.06 to 0.55 and in general, heritabilities for NAb isotypes were higher than heritabilities for total NAb levels, the latter making no distinction between isotypes. Genetic correlations suggest that isotypes IgA and IgM have a common genetic basis, but that the genetic basis for IgG1 differs from that for IgA or IgM. An additional genome-wide association study for NAb levels showed that information can be gained when total NAb levels are further subdivided into isotype levels. A region on chromosome 23 was significantly associated with genetic variation in isotype IgM levels. The bovine major histocompatibility complex (MHC) is located near this region, making this a region of candidate gene(s) involved in NAb expression in dairy cows both from a functional and positional perspective. Results from the study on genetic parameters and the genome-wide association study suggest that NAb isotypes may provide a better characterization of different elements of the immune response or immune competence and enable more effective decisions when breeding programs start to include innate immune parameters. A genome-wide association study was not only performed for NAb levels, but also for SCS. Relatively few associations, however, were found, which suggests that SCS is controlled by multiple loci, each with a relatively small effect, distributed across the genome.
Somatic cell score is partly under genetic control, but is also affected by the environment. Sensitivity to respond to environmental factors, however, can have a genetic origin.
Environmental factors can be divided into known and unknown factors, referred to as macro- and micro environment, respectively. Macro-environmental sensitivity can be expressed as genetic variation in the slope of a reaction norm, whereas micro-environmental sensitivity can be expressed as differences in residual variance that have a genetic origin. Both macro- and micro-environmental sensitivity were found for SCS and these sensitivities were positively correlated. Knowledge on both forms of sensitivity can aid in optimization of selection as correlations between the additive genetic variance in intercept, slope and environmental variance were all away from unity. Selection for reduced environmental sensitivity has the potential to reduce variability in animal performance due to environmental factors and herewith increase predictability of performance across and within environments.
Knowledge on disease biology is important to fully understand the processes involved when selecting for increased disease resistance, as a better understanding enables a better prediction of the consequences. In this context, the general discussion involved the phenotype definition and statistical modeling, influence of maternal effects and genetic variation in the MHC region. The discussion contained three conclusions: 1) analyses of cell types (detailed phenotypes) rather than SCS can provide further insight in the genetic control of SCS and mastitis; 2) no evidence was found for maternal genetic effects on NAb levels in milk. Maternal environmental effects, however, could play a role in NAb levels; 3) genetic diversity in the MHC region is maintained by natural selection. Selective breeding and farm management practices may affect this genetic diversity, which could bring about negative effects on animal fitness, such as fertility problems. Selective breeding for specific MHC haplotypes may therefore impose a risk for negative effects on animal health.
|Longitudinal analyses of indoor temperature and BMI in children in the PIAMA birth cohort study
Scheffers, F.R. ; Bekkers, M.B.M. ; Kerkhof, M.F. ; Gehring, U. ; Koppelman, G.H. ; Schipper, M. ; Haveman-Nies, A. ; Wijga, A.H. - \ 2012
Maternal use of folic acid supplements during pregnancy, and childhood respiratory health and atopy
Bekkers, M.B.M. ; Elstgeest, L.E.M. ; Scholtens, S. ; Haveman-Nies, A. ; Jongste, J.C. de; Kerhof, M. ; Koppelman, G.H. ; Gehring, U. ; Smit, H.A. ; Wijga, A.H. - \ 2012
European Respiratory Journal 39 (2012)6. - ISSN 0903-1936 - p. 1468 - 1474.
birth cohort - asthma - risk - disorders
Previous studies have suggested possible adverse side-effects of maternal use of folic acid-containing supplements (FACSs) during pregnancy on wheeze and asthma in early childhood. We investigated the association between maternal use of FACSs and childhood respiratory health and atopy in the first 8 yrs of life. Data on maternal use of FACSs, collected during pregnancy, were available for 3,786 children participating in the Prevention and Incidence of Asthma and Mite Allergy birth cohort study. Questionnaire data on children’s respiratory and allergic symptoms were collected annually and allergic sensitisation and bronchial hyperresponsiveness (BHR) were measured at 8 yrs of age. No overall (from 1 to 8 yrs of age) associations were observed between maternal use of FACSs and (frequent) asthma symptoms, wheeze, lower respiratory tract infection, frequent respiratory tract infection and eczema. Maternal folic acid use was associated with wheeze at 1 yr of age (prevalence ratio 1.20, 95% CI 1.04–1.39), but not with wheeze at later ages. Pre-natal exposure to FACSs was not associated with sensitisation and BHR. Apart from a small increased risk of early wheeze, we observed no adverse respiratory or allergic outcomes associated with pre-natal FACSs exposure in our study population
Genome-wide associations for fertility traits in Holstein–Friesian dairy cows using data from experimental research herds in four European countries
Berry, D.P. ; Bastiaansen, J.W.M. ; Veerkamp, R.F. ; Wijga, S. ; Wall, E. ; Berglund, B. ; Calus, M.P.L. - \ 2012
Animal 6 (2012)8. - ISSN 1751-7311 - p. 1206 - 1215.
linkage disequilibrium - luteal activity - milk-yield - cattle - selection - accuracy - information - haplotypes - endocrine - system
Genome-wide association studies for difficult-to-measure traits are generally limited by the sample population size with accurate phenotypic data. The objective of this study was to utilise data on primiparous Holstein–Friesian cows from experimental farms in Ireland, the United Kingdom, the Netherlands and Sweden to identify genomic regions associated with traditional measures of fertility, as well as a fertility phenotype derived from milk progesterone profiles. Traditional fertility measures investigated were days to first heat, days to first service, pregnancy rate to first service, number of services and calving interval (CI); post-partum interval to the commencement of luteal activity (CLA) was derived using routine milk progesterone assays. Phenotypic and genotypic data on 37 590 single nucleotide polymorphisms (SNPs) were available for up to 1570 primiparous cows. Genetic parameters were estimated using linear animal models, and univariate and bivariate genome-wide association analyses were undertaken using Bayesian stochastic search variable selection performed using Gibbs sampling. Heritability estimates of the traditional fertility traits varied from 0.03 to 0.16; the heritability for CLA was 0.13. The posterior quantitative trait locus (QTL) probabilities, across the genome, for the traditional fertility measures were all
Genomic associations with somatic cell score in first-lactation Holstein cows
Wijga, S. ; Bastiaansen, J.W.M. ; Wall, E. ; Strandberg, E. ; Haas, Y. de - \ 2012
Journal of Dairy Science 95 (2012)2. - ISSN 0022-0302 - p. 899 - 908.
quantitative trait loci - affecting clinical mastitis - dairy-cattle breeds - 1st 3 lactations - milk-production - genetic-parameters - escherichia-coli - wide association - health traits - count traits
This genome-wide association study aimed to identify loci associated with lactation-average somatic cell score (LASCS) and the standard deviation of test-day somatic cell score (SCS-SD). It is one of the first studies to combine detailed phenotypic and genotypic cow data from research dairy herds located in different countries. The combined data set contained up to 52 individual test-days per lactation and thereby aimed to capture temporary increases in somatic cell score associated with infection. Phenotypic data for analysis consisted of 46,882 test-day records on 1,484 cows, and genotypic data consisted of 37,590 single nucleotide polymorphisms (SNP). Using an animal model, the associations between each individual SNP and the phenotypic data were estimated. To account for the risk of false positives, a false discovery rate threshold of 0.20 was set. The analyses showed that LASCS was significantly associated with a SNP on Bos taurus autosome (BTA) 4 and a SNP on BTA18. Likewise, SCS-SD was associated with this SNP on BTA18. In addition, SCS-SD significantly associated with a SNP on BTA6. Relatively few associations were found, suggesting that LASCS and SCS-SD are controlled by multiple loci distributed across the genome, each with a relatively small effect. Increased knowledge on genetic regulation of LASCS and SCS-SD may aid in identification of genes that play a role in mastitis resistance. Such knowledge helps us understand the genetic mechanisms leading to mastitis and in discovery of targets for mastitis therapeutics.
Overweight and School Performance Among Primary School Children: The PIAMA Birth Cohort Study
Veldwijk, J. ; Fries, M.C.E. ; Bemelmans, W.J.E. ; Haveman-Nies, A. ; Smit, H.A. ; Koppelman, G.H. ; Wijga, A.H. - \ 2012
Obesity 20 (2012)3. - ISSN 1930-7381 - p. 590 - 596.
academic-performance - childhood overweight - physical-activity - weight status - body-weight - obesity - adolescents - consequences - health - risk
The aim of this study was to assess the association between overweight and school performance among primary school children prospectively and including a broad range of potential confounding factors. In addition it was investigated what factors mediate this association. For this purpose, data of 2,159 12-year-old children who participated in the Prevention and Incidence of Asthma and Mite Allergy (PIAMA) birth cohort study were used. Two indicators of school performance were parental reported when children were 12 years of age and included (i): the score on a standardized achievement test that Dutch children have to complete at the end of their primary education (Cito)-test and (ii): the teacher's advice regarding a child's potential performance level in secondary education. Children's height and weight were measured by a trained research assistant at the age of 8 and by their parents at the age of 12. Overweight was defined using age and gender specific cut-off points. Multivariate regression analyses were performed to assess the association between overweight and school performance. Besides, both confounder and mediation analyses were conducted. Results showed lower Cito-test scores and lower teacher's school-level advice among overweight children. These associations were no longer significant when adjusting for parental educational level, skipping breakfast, and screen time. This study found no independent association between overweight and school performance among primary school children. Results showed strong confounding by parental educational level
GWAS for Robustness Traits
Bovenhuis, H. ; Berry, D.P. ; Lunden, A. ; Wall, E. ; Bastiaansen, J. ; Wijga, S. ; Calus, M.P.L. ; Veerkamp, R.F. - \ 2011
In: Proceedings of the 2011 Interbull meeting, 26-29 August 2011, Stavanger, Norway. - - p. 216 - 219.
|Genome wide association mapping in multiple cow populations of the RobustMilk project
Bastiaansen, J.W.M. ; Bovenhuis, H. ; Wijga, S. ; Mc Parland, S. ; Wall, E. ; Strandberg, E. ; Veerkamp, R.F. - \ 2011
RobustMilk is a 4 year collaborative research project that started in 2008 with funding from the EU Framework 7 Programme. The objective of RobustMilk is to develop new useful and practical technologies to allow dairy farmers and the dairy industry to refocus their selection decisions to include additional traits such as milk quality and dairy cow robustness. As part of the project data and DNA samples were combined from 2,000 cows in research herds in 4 different countries. Combining these datasets for genome wide association analysis was investigated and associations were estimated along the genome using 50k genotypes for production and robustness traits.
|Genomic regions associated with somatic cell score in dairy cattle
Wijga, S. ; Bastiaansen, J.W.M. ; Wall, E. ; Strandberg, E. ; Haas, Y. de; Bovenhuis, H. - \ 2011
In: Proceedings of the International Conference on Udder Health and Communication, Utrecht, the Netherlands, 25-27 October 2011. - Wageningen Academic Publishers - ISBN 9789086861859 - p. 373 - 380.
The EU project RobustMilk combines unique data from research dairy herds located in four European countries. The dataset contains detailed recordings on somatic cell score (SCS), with up to 50 SCS test-days per cow per lactation. Variation in SCS can be due to genetic and environmental factors. The aim of the present study was to identify regions on the genome that contribute to genetic variation in lactation-average SCS (LASCS) and the standard deviation of SCS (SCS-SD). The standard deviation of SCS aims to capture temporary increases in SCS associated with infection. First lactation SCS records and DNA of 1,484 cows were analysed. Cows were genotyped for 50,000 markers. Each individual marker was tested for its detection of variation in LASCS or SCS-SD. One marker on chromosome (BTA) 18 detected variation in both LASCS and SCS-SD. One marker on BTA4 detected variation in LASCS, and one marker on BTA6 detected variation in SCS-SD. The present study identified genomic regions on BTA4, BTA6 and BTA18 contributing to genetic variation in LASCS and SCS-SD. More knowledge on genetic control of LASCS and SCS-SD may not only enable more accurate breeding value estimations that allow farmers and the dairy industry to reassess their selection decisions, but also helps to find genes for mastitis resistance which is relevant for understanding the genetic mechanisms leading to mastitis.
Genomic regions associated with natural antibody titres in milk of Dutch Holstein-Friesian cows
Wijga, S. ; Bastiaansen, J.W.M. ; Ploegaert, T.C.W. ; Arendonk, J.A.M. van; Poel, J.J. van der - \ 2011
In: Book of Abstracts of the 62nd Annual Meeting of the European Federation of Animal Science, Stavanger, Norway, 29 August - 2 September 2011. - Wageningen : Wageningen Academic Publishers - ISBN 9789086867318 - p. 32 - 32.
Serum micronutrient concentrations and childhood asthma: the PIAMA birth cohort study
Oeffelen, A.A.M. van; Bekkers, M.B.M. ; Smit, H.A. ; Kerkhof, M. van de; Koppelman, G.H. ; Haveman-Nies, A. ; A, D.L. van der; Jansen, E.H.J.M. ; Wijga, A.H. - \ 2011
Pediatric Allergy and Immunology 22 (2011)8. - ISSN 0905-6157 - p. 784 - 793.
nutrition examination survey - vitamin-d levels - zinc status - trace-elements - t-lymphocytes - smooth-muscle - children - magnesium - selenium - cells
Background: Research suggests an influence of micronutrients on childhood asthma. So far, evidence mainly originates from cross-sectional studies using nutrient intake data, which is not an accurate measure of nutrient status. This study aimed to investigate the cross-sectional and prospective associations between serum concentrations of magnesium, vitamin D, selenium, and zinc and prevalence of (severe) asthma, atopy, and bronchial hyperresponsiveness (BHR) in childhood. Methods: In the Prevention and Incidence of Asthma and Mite Allergy birth cohort study, serum nutrient concentrations were available for a 4-yr-old subgroup (n = 372) and for a different 8-yr-old subgroup (n = 328). Yearly questionnaires inquired about asthma prevalence until 8 yr of age. Allergic sensitization was measured at 4 and 8 yr of age; BHR was measured at 8 yr of age. Data were analyzed with logistic regression and generalized estimating equations models. Results: There was a consistent (non-significant) inverse association between serum magnesium concentrations and asthma prevalence. Serum vitamin D concentrations measured at age 4 were inversely associated with asthma at ages 4–8 [e.g., cross-sectional association between vitamin D tertile 3 vs. 1 and severe asthma: odds ratio (OR): 0.49, 95% confidence interval (CI): 0.25–0.95], whereas vitamin D measured at age 8 was positively associated with asthma at age 8 (e.g., cross-sectional association between vitamin D tertile 3 vs. 1 and severe asthma: OR: 2.14, 95% CI: 0.67–6.82). Conclusions: Our study contributes to the evidence that children with higher serum magnesium concentrations are less likely to have asthma. The associations between serum vitamin D concentrations and asthma were age-dependent