Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Ontwikkeling van een effectieve methode om glastuinbouwproducten insectenvrij en met verbeterde milieu- en productkwaliteit te kunnen exporteren
    Gelder, A. de; Wijkamp, M.G. ; Smid, E.J. - \ 1998
    Aalsmeer : Proefstation voor Bloemisterij en Glasgroente - 65
    Methods to rear and maintain thrips in tospovirus transmission studies.
    Peters, D. ; Loomans, A. ; Nagata, T. ; Wijkamp, I. ; Wetering, F. van de - \ 1998
    In: Invertebrates in Captivity Conference TM Transmission studies, Tucson, Arizona, USA - p. 152 - 159.
    Ontwikkeling van een effectieve methode om glastuinbouwproducten insectenvrij en met verbeterde milieu- en productkwaliteit te kunnen exporteren
    Gelder, A. de; Wijkamp, M.G. ; Smid, E.J. - \ 1997
    Aalsmeer : Proefstation voor Bloemisterij en Glasgroente - 85
    Detection of trace gases from long term stored fruit and respiration of insects
    Harren, F.J.M. ; Oomens, J. ; Persijn, S. ; Wijkamp, I. ; Veltman, R.H. ; Schaik, A.C.R. van; Vries, H.S.M. de; Parker, D.H. - \ 1997
    In: Proceeding of Lasers in Medicine and Biology; LEOS 97, San Francisco CA, USA - p. 556 - 557.
    Transmission of tomato spotted wilt virus by Frankliniella occidentalis; median acquisition and inoculation period.
    Wijkamp, I. ; Wetering, F. van de; Goldbach, R. ; Peters, D. - \ 1996
    Annals of Applied Biology 129 (1996). - ISSN 0003-4746 - p. 303 - 313.
    Molecular basis of Tospovirus transmission by thrips.
    Goldbach, R.W. ; Wijkamp, I. ; Wetering, F. van de; Peters, D. - \ 1996
    In: Abstract 20th Int. Congr. of Entomology, Florence, Italy - p. 454 - 454.
    Virus/vector/plant relations in the epidemiology of tospoviruses.
    Peters, D. ; Wijkamp, I. ; Wetering, F. van de; Goldbach, R. - \ 1996
    In: Abstract 20th Int. Congr. of Entomology, Florence, Italy - p. 456 - 456.
    Vector relations in the transmission and epidemiology of Tospoviruses.
    Peters, D. ; Wijkamp, I. ; Wetering, F. van de; Goldbach, R. - \ 1996
    Acta Horticulturae 431 (1996). - ISSN 0567-7572 - p. 29 - 43.
    Tospoviruses are now recognized worldwide as limiting factors in the production of a large number of horticultural crops. The economic impact of the type species of these viruses, the tomato spotted wilt virus (TSWV), seems to be the highest because of its very broad host range, the current worldwide spread one of its most common vectors, the western flower thrips (Frankliniella occidentalis) and the abundance of Thrips tabaci in some solanaceous crops. The economic impact of recently described tospovirus species must still be assessed using reliable diagnostic tools. The spread of the tospoviruses and infection of the plant results from interactions between the plant, the vector and the virus. To better understand the epidemiology of tospoviruses, their interactions with various thrips species are reported.
    Propagation of tomato spotted wilt virus in Frankliniella occidentalis does neither result in pathological effects nor in transovarial passage of the virus.
    Wijkamp, I. ; Goldbach, R. ; Peters, D. - \ 1996
    Entomologia Experimentalis et Applicata 81 (1996). - ISSN 0013-8703 - p. 285 - 292.
    Differential susceptibilities between leaf disks and plants in the transmission of tomato spotted wilt virus by Frankliniella occidentalis to TSWV hosts and transgenic plants.
    Wijkamp, I. ; Goldbach, R. ; Peters, D. - \ 1996
    Journal of Phytopathology 144 (1996). - ISSN 0931-1785 - p. 355 - 362.
    Multiplication of tomato spotted wilt virus in western flower thrips.
    Wijkamp, I. ; Lent, J. van; Kormelink, R. ; Goldbach, R. ; Peters, D. - \ 1995
    In: Thrips biology and management / Parker, B.L., New York : Plenum Press - p. 157 - 161.
    Median latent period and transmission of tospoviruses vectored by thrips.
    Wijkamp, I. ; Almarza, N. ; Peters, D. - \ 1995
    In: Thrips biology and management / Parket, B.L., New York : Plenum Press - p. 153 - 156.
    Distinct levels of specificity in thrips transmission of tospoviruses.
    Wijkamp, I. ; Almarza, N. ; Goldbach, R. ; Peters, D. - \ 1995
    Phytopathology 85 (1995). - ISSN 0031-949X - p. 1069 - 1074.
    Effect of tomato spotted wilt virus infection on survival, development and reproduction of the vector Frankliniella occidentalis.
    Wijkamp, I. ; Goldbach, R.W. ; Peters, D. - \ 1995
    Proceedings of the Section Experimental and Applied Entomology of the Netherlands Entomological Society 6 (1995). - ISSN 1388-8390 - p. 207 - 214.
    Virus - vector relationships in the transmission of tospoviruses
    Wijkamp, I. - \ 1995
    Agricultural University. Promotor(en): R.W. Goldbach; D. Peters. - S.l. : Wijkamp - ISBN 9789054854487 - 143
    plantenziekten - plantenziekteverwekkende bacteriën - tomatenbronsvlekkenvirus - plantenplagen - epidemiologie - distributie - virussen - vectoren - psocoptera - plant diseases - plant pathogenic bacteria - tomato spotted wilt virus - plant pests - epidemiology - distribution - viruses - vectors - psocoptera

    Tomato spotted wilt virus (TSWV), member of the genus Tospovirus within the family Bunyaviridae, ranks among the top ten of economically most important plant viruses. Tospoviruses cause significant yield losses in agricultural crops such as tomato, lettuce, pepper, tobacco, potato and groundnut, but also in ornamentals like chrysanthemum, alstroemeria, gloxinia and impatiens. Currently, more than 650 different plant species belonging to more than 70 distinct botanical families are known to be susceptible to tospoviruses. Following the introduction of the vector Frankliniella occidentalis from the United states in Europe, TSWV and impatiens necrotic spot virus (INSV), a tospovirus mainly occurring in ornamentals, are prevailing in the Netherlands, especially in greenhouse cultivations.

    To gain more insight in the epidemiology of tospoviruses, information is needed on the kinetics of their transmission. The experiments described in this thesis were done to analyze some of the parameters involved.

    An efficient local lesion assay was developed to facilitate transmission studies using leaf disks of Petunia x hybrida "Blue Magic". In this assay, transmission could be scored reliably within 2 to 3 days on the basis of local lesion formation. This assay also enabled easy handling of large numbers of thrips. Besides petunia, leaf disks of other plant hosts were found to be suitable as substrate in transmission studies as well.

    Using this assay it was established that F. occidentalis could acquire and transmit the virus in periods of 5 min. Longer periods were required for more efficient transmission. To quantify virus transmission the median acquisition (AAP 50 ) and inoculation access periods (IAP 50 ), i.e. the periods needed for 50% of the thrips to respectively acquire or inoculate the virus were determined. The value for the AAP 50 was 67 min whereas an IAP 50 of 59 min was found. The leaf disk assay was also employed to determine the efficiency of transmission and the latent period (LP), the period between acquisition and inoculation of the virus, for both TSWV and INSV. The majority of thrips already transmitted virus at the end of the second larval stage at transmission rates for TSWV or INSV of 52.8 % or 80.0%, respectively.

    The fate of the virus in the thrips after ingestion was studied in different developmental stages of F. occidentalis. The accumulation of two viral proteins, the nucleocapsid (N) and a non-structural (NS S ) protein, as monitored by ELISA en Western blot analyses, increased within two days above the levels initially ingested. Immunocytology of infected adults confirmed that viral products were present in high amounts, especially, in the salivary glands. Electron microscopic studies revealed the presence of many virus particles in the salivary ducts. These results unequivocally demonstrated that TSWV replicated in its insect vector and that the salivary glands were a major site of multiplication. This multiplication was not accompanied by pathological effects on the vector. No effect of virus infection was found on development time, reproduction rate and survival . This study al so revealed that no transovarial transmission of virus to the progeny took place.

    The vector competence was determined for four thrips and four tospovirus species. The results showed that specificity of transmission, as occurring for other insect-transmitted plant viruses, also exists for tospovirus transmission by thrips. F. occidentalis appeared to be the most efficient vector for all 4 viruses tested, i.e. TSWV, INSV, tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). Frankliniella schultzei transmitted three (TSWV, TCSV and GRSV) of the four tospoviruses, whereas Frankliniella intonsa, not yet reported as tospovirus vector, transmitted TSWV and TCSV. Of Thripstabaci, previously known as an important vector for TSWV, only one out of four populations tested was able to transmit TSWV at, moreover, a low efficiency.

    Finally, the transmission of mutant tospoviruses, which were generated after successive mechanical transfers of virus, was studied. Defective interfering mutants were found to be transmittable by thrips, albeit at a frequency 10 to 20 times lower than the wild type virus. In contrast, envelope deficient mutants were not transmitted by thrips, indicating the importance of the envelope glycoproteins in virus-vector relationships.

    Tomato spotted wilt virus: genome organization, transmission, and symptom induction.
    Goldbach, R. ; Kormelink, R. ; O. Resende, R. de; Avila, A.C. de; Poelwijk, F. van; Lent, J. van; Wijkamp, I. ; Prins, M. ; Peters, D. - \ 1995
    In: Biotechnology and plant protection / Bills, D.D., Kung, Shain-dow, - p. 297 - 311.
    Functional analyses on the nonstructural proteins of tomato spotted wilt virus.
    Kormelink, R. ; Storms, M. ; Wijkamp, I. ; Lent, J. van; Peters, D. ; Goldbach, R. - \ 1995
    In: Assoc. of Applied Biologists, Cambridge, UK (1995)
    Multiplication of tomato spotted wilt virus in its vector Frankliniella occidentalis (Thysanoptera: Thripidae).
    Wijkamp, M.G. - \ 1994
    In: PhD Students Meeting C.T. de Wit Graduate School Production Ecology (1994)
    Thrips transmission of tospoviruses containing defective interfering RNAs.
    Wijkamp, I. ; Duineveld, B. ; Goldbach, R. ; Peters, D. - \ 1994
    In: Am. Soc. Virology, 13th Ann. Meeting, Univ. Wisconsin-Madison, USA - p. 219 - 219.
    Virus-vector interactions during the transmission of two tospoviruses by Frankliniella occidentalis.
    Wijkamp, I. ; Lent, J. van; Kormelink, R. ; Goldbach, R. ; Peters, D. - \ 1993
    In: Abstract 9th Int. Congr. Virology, Glasgow, UK - p. 102 - 102.
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