Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Gene profiling-based phenotyping for identification of cellular parameters that contribute to fitness, stress-tolerance and virulence of Listeria monocytogenes variants
    Koomen, J.S. ; Besten, H.M.W. den; Metselaar, K.I. ; Tempelaars, M.H. ; Wijnands, L.M. ; Zwietering, M.H. ; Abee, T. - \ 2018
    Wageningen University
    GSE114672 - PRJNA472154 - Listeria monocytogenes
    Microbial population heterogeneity allows for a differential microbial response to environmental stresses and can lead to the selection of stress resistant variants. In this study, we have used two different stress resistant variants of Listeria monocytogenes LO28 with mutations in the rpsU gene encoding ribosomal protein S21, to elucidate features that can contribute to fitness, stress-tolerance and host interaction using a comparative gene profiling and phenotyping approach. Transcriptome analysis showed differential expression of 319 genes of which 116 genes were upregulated and 114 genes were downregulated in both rpsU variants. Upregulated genes included a major contribution of SigB-controlled genes such as intracellular acid resistance-associated glutamate decarboxylase (GAD) (gad3), genes involved in compatible solute uptake (opuC), glycerol metabolism (glpF, glpK, glpD), and virulence (inlA, inlB). Downregulated genes in the two variants involved mainly genes involved in flagella synthesis and motility. Phenotyping results of rpsU variants matched the gene profiling data including enhanced freezing resistance conceivably linked to compatible solute accumulation, higher glycerol utilisation rates, and better adhesion to Caco 2 cells presumably linked to higher expression of internalins. Also, bright field and electron microscopy analysis confirmed reduced flagellation of the variants. The activation of SigB-mediated stress defence offers an explanation for the multiple-stress resistant phenotype in rpsU variants.
    Assessing sustainable food and nutrition security of the EU food system - An integrated approach
    Zurek, Monika ; Hebinck, Aniek ; Leip, Adrian ; Vervoort, Joost ; Kuiper, Marijke ; Garrone, Maria ; Havlík, Petr ; Heckelei, Thomas ; Hornborg, Sara ; Ingram, John ; Kuijsten, Anneleen ; Shutes, Lindsay ; Geleijnse, Johanna M. ; Terluin, Ida ; Veer, Pieter van 't; Wijnands, Jo ; Zimmermann, Andrea ; Achterbosch, Thom - \ 2018
    Sustainability 10 (2018)11. - ISSN 2071-1050
    Food system assessment - Food systems - Interdisciplinarity - Metrics - Participatory approach - SUSFANS - Sustainable food and nutrition security

    Steering the EU food system towards a sustainability transformation requires a vast and actionable knowledge base available to a range of public and private actors. Few have captured this complexity by assessing food systems from a multi-dimensional and multi-level perspective, which would include (1) nutrition and diet, environmental and economic outcomes together with social equity dimensions and (2) system interactions across country, EU and global scales. This paper addresses this gap in food systems research and science communication by providing an integrated analytical approach and new ways to communicate this complexity outside science. Based on a transdisciplinary science approach with continuous stakeholder input, the EU Horizon2020 project 'Metrics, Models and Foresight for European SUStainable Food And Nutrition Security' (SUSFANS) developed a five-step process: Creating a participatory space; designing a conceptual framework of the EU food system; developing food system performance metrics; designing a modelling toolbox and developing a visualization tool. The Sustainable Food and Nutrition-Visualizer, designed to communicate complex policy change-impacts and trade-off questions, enables an informed debate about trade-offs associated with options for change among food system actors as well as in the policy making arena. The discussion highlights points for further research related to indicator development, reach of assessment models, participatory processes and obstacles in science communication.

    Using a system innovation's approach for stimulating agroforestry adoption
    Cuperus, Fogelina ; Schoutsen, M.A. ; Sukkel, W. ; Selin Norén, Isabella ; Wijnands, F.G. - \ 2018
    In: Agroforestry as sustainable land use. - - p. 35 - 39.
    Gene profiling-based phenotyping for identification of cellular parameters that contribute to fitness, stress-tolerance and virulence of Listeria monocytogenes variants
    Koomen, Jeroen ; Besten, Heidy M.W. den; Metselaar, Karin I. ; Tempelaars, Marcel H. ; Wijnands, Lucas M. ; Zwietering, Marcel H. ; Abee, Tjakko - \ 2018
    International Journal of Food Microbiology 283 (2018). - ISSN 0168-1605 - p. 14 - 21.
    Listeria monocytogenes - rpsU - SigB - Stress resistant variants

    Microbial population heterogeneity allows for a differential microbial response to environmental stresses and can lead to the selection of stress resistant variants. In this study, we have used two different stress resistant variants of Listeria monocytogenes LO28 with mutations in the rpsU gene encoding ribosomal protein S21, to elucidate features that can contribute to fitness, stress-tolerance and host interaction using a comparative gene profiling and phenotyping approach. Transcriptome analysis showed that 116 genes were upregulated and 114 genes were downregulated in both rpsU variants. Upregulated genes included a major contribution of SigB-controlled genes such as intracellular acid resistance-associated glutamate decarboxylase (GAD) (gad3), genes involved in compatible solute uptake (opuC), glycerol metabolism (glpF, glpK, glpD), and virulence (inlA, inlB). Downregulated genes in the two variants involved mainly genes involved in flagella synthesis and motility. Phenotyping results of the two rpsU variants matched the gene profiling data including enhanced freezing resistance conceivably linked to compatible solute accumulation, higher glycerol utilisation rates, and better adhesion to Caco 2 cells presumably linked to higher expression of internalins. Also, bright field and electron microscopy analysis confirmed reduced flagellation of the variants. The activation of SigB-mediated stress defence offers an explanation for the multiple-stress resistant phenotype in rpsU variants.

    Options for sustaining solar-powered mosquito trapping systems on Rusinga Island, Western Kenya : A social dilemma analysis
    Oria, Prisca A. ; Wijnands, Michiel ; Alaii, Jane ; Leeuwis, Cees - \ 2018
    BMC Public Health 18 (2018)1. - ISSN 1471-2458
    Community - Kenya - Malaria - Mosquito - Social dilemma - Solar - Sustainability - Traps
    Background: In 2012, a donor-supported proof of principle study was launched to eliminate malaria from Rusinga Island, western Kenya, using solar-powered mosquito trapping systems (SMoTS). SMoTS, which also provided power for room lighting and charging mobile telephones, were installed in houses. In view of the involvement of individual and collective benefits, as well as individual and collective maintenance solutions, this study qualitatively examined preferences of some project stakeholders towards SMoTS sustainability components to see if and how they related to social dilemma factors. Methods: The data were collected through participant observation, semi-structured interviews and focus group discussions. Results: The results show that respondents largely preferred individual solutions to various aspects of maintenance. Selective collective solutions such as table banking groups were considered positively for mobilising financial resources for maintenance, but respondents were hardly willing to contribute financially to a collective entity. Few people saw a meaningful role for a collective governing body; people preferred to rely on individual household responsibility and private service delivery for repairs and stocking spare parts. An overriding concern was that people lacked trust in other community members, leaders and/or technicians who would be employed by a governing body. Respondents also had little confidence that a governing body or saving group could effectively impose sanctions to misappropriation of funds, poor leadership, defecting group members or technicians that might abuse a salaried position. Conclusion: There seemed to be linkages between preferences towards organising various components of SMoTS sustainability and known hindrances to addressing social dilemmas. This posed considerable challenges to organising the sustainability of this innovative malaria control strategy. Trial registration: NTR3496.
    Gene profiling-based phenotyping for identification of cellular parameters that contribute to fitness, robustness and virulence of acid resistant Listeria monocytogenes variants
    Koomen, J.S. ; Metselaar, K.I. ; Tempelaars, M.H. ; Wijnands, Lucas M. ; Zwietering, M.H. ; Besten, H.M.W. den; Abee, T. - \ 2017
    Analyses of the Functioning of Milk Package provisions as regards Producer Organisations and collective negotiations
    Wijnands, J.H.M. ; Bijman, J. ; Tramnitzke, Tanja - \ 2017
    Luxembourg : European Union (JRC technical reports ) - ISBN 9789279731884 - 130 p.
    milk package producer organisation contracting
    This study aims to assess the effectiveness and potential improvement of the Milk Package provisions, as regards the functioning of producer organisations (POs) and their role in collective negotiations with milk processors. The specific objectives are to: 1. Evaluate the functioning of existing POs and associations of producer organisations (APOs) formally recognised under the Milk Package. 2. Evaluate the potential for POs where they have not been constituted yet. 3. In the light of the analysis, draw up recommendations to improve the usefulness of the Milk Package provisions. Legislation on POs in the dairy sector is provided in the Common Market Organisation Regulation (Regulation (EU) 1308/2013). According to this Regulation, Member States shall recognise POs in the milk sector formed on the initiative of producers and pursuing a specific aim, which may include (Article 152(3) of Regulation 1308/2013): (i) ensuring that production is planned and adjusted to demand, particularly in terms of quantity and quality; (ii) concentration of supply and the placing on the market of products produced by its members; (iii) optimising production costs and stabilising producer prices.
    Verbindend ondernemen : Betekenisvolle biologische sector, vandaag en (over)morgen
    Dagevos, Hans ; Wijnands, Frank ; Meeusen, Marieke - \ 2017
    Wageningen : Wageningen University & Research - 29
    biologische landbouw - ondernemerschap - maatschappelijk verantwoord ondernemen - organic farming - entrepreneurship - corporate social responsibility
    In dit ‘inspiratieboekje’ Verbindend ondernemen ligt de nadruk op conceptueel-theoretische noties en redeneerlijnen die de bedoeling hebben de sociale dimensie van duurzaamheid – de P van people – perspectief en inhoud te geven.
    Deliverable No. 1.3: Sustainability metrics for the EU food system: a review across economic, environmental and social considerations
    Zurek, Monika ; Leip, Adrian ; Kuijsten, Anneleen ; Wijnands, Jo ; Terluin, Ida ; Shutes, Lindsay ; Hebinck, Aniek ; Zimmermann, Andrea ; Götz, Christian ; Hornborg, Sara ; Zanten, Hannah van; Ziegler, Friederike ; Havlik, Petr ; Garrone, Maria ; Geleijnse, Marianne ; Kuiper, Marijke ; Turrini, Aida ; Dofkova, Marcela ; Trolle, Ellen ; Mistura, Lorenza ; Dubuisson, Carine ; Veer, Pieter van 't; Achterbosch, Thom ; Ingram, John ; Brem-Wilson, Joshua ; Franklin, Alex ; Fried, Jana ; Guzman Rodriguez, Paola ; Owen, Luke ; Saxena, Lopa ; Trenchard, Liz ; Wright, Julia - \ 2017
    SUSFANS - 78
    One of the main objectives of the SUSFANS project is to develop a set of concepts and tools to help policy and decision makers across Europe make sense of the outcomes and trends of the EU food system. This paper proposes a set of metrics for assessing the performance of the EU food system in delivering sustainable food and nutrition security. The performance metrics have been built up through the aggregation of a wide range of variables, which together help to monitor the achievement of four overarching policy goals for the EU food system, namely a balanced diet for EU citizens, reduced environmental impacts, competitive agri-food businesses and equitable outcomes of the food system. The project decided to take a hierarchical approach to aggregating from Individual Variables to Derived Variables to Aggregate Indicators to Performance Metrics. This approach aims at marrying the notion that decision makers want only a small but powerful set of metrics to communicate the findings of the assessment, with the need to substantiate these metrics with the best available data from a large number of sources in a transparent way. In this deliverable the current set up of the performance metrics focus on each individual policy goal. In a related report, the team explores if and how the performance metrics presented here can be quantified using available data and modelling tools, and which of the models of the SUSFANS tool box can estimate which ones of the performance metrics and how (report D1.4). In a final step the SUSFANS team will bring all performance metrics together in an integrated set that will allow a view across all four policy goals and thus across all aspects of sustainable food and nutrition security (forthcoming report D1.5). Further work is the quantification of metrics using case studies and prospective scenario analysis. In addition to their use for monitoring, the proposed metrics are geared towards quantification using selected computational modelling tools. As such, SUSFANS aims to assist in foresight on and the evaluation of transformative changes in the food system with rigour and consistency.
    Competitiveness of agri-food chains in EU’s Eastern Neighbours. Quantifying Porter’s diamond
    Berkum, S. van; Wijnands, J.H.M. ; Verhoog, A.D. - \ 2016
    - 14 p.
    Duurzame energie 2050 : verkenning rol van (agrarische) ondernemers in de energietransitie naar 2050
    Wijnands, Frank ; Holster, Henri - \ 2016
    Wageningen : Praktijkonderzoek Plant & Omgeving, onderdeel van Wageningen UR - 33
    boeren - plattelandsomgeving - duurzame energie - energie - energievoorraden - hernieuwbare energie - farmers - rural environment - sustainable energy - energy - energy resources - renewable energy
    Rural entrepreneurs (farmers) are going to play a major role in the energy transitions towards 2050. In professional dialogues between farmers and pertinent stakeholders it was shown that farmers are already involved in all aspects and are in the right position. The transition needs to be facilitated in local initiatives.
    Kansen voor de Nederlandse dierlijke agrosector in Japan : notitie voor de Topsector Agri & Food, thema Internationalisering
    Berkum, Siemen ; Wijnands, Jo H.M. - \ 2016
    Wageningen : Wageningen Economic Research (Wageningen Economic Research nota 2016-125) - 31
    Competitiveness of the EU food industry : ex-post assessmentof trade performance embedded in international economic theory
    Wijnands, J.H.M. ; Verhoog, A.D. - \ 2016
    Wageningen : LEI Wageningen UR (Report / LEI Wageningen UR 2016-018) - ISBN 9789086157358 - 79
    food industry - market competition - competitive ability - terms of trade - economic indicators - international trade - world markets - european union - usa - voedselindustrie - marktconcurrentie - concurrerend vermogen - ruilvoet - economische indicatoren - internationale handel - wereldmarkten - europese unie - vs
    This study assessed the competitiveness of the EU food industry benchmarked against USA, Australia, Brazil and Canada. This assessment is based on trade indicators (Relative Net trade advantage and export share on the world market) and on economic indicators (value added, labour productivity and share value added in total manufacturing). This study is an update of an earlier study executed in 2007. The overall competitiveness performance of the EU28 (intra-EU trade excluded) remained weak: the three economic indicators weakened and the trade indicators improved in period (2) 2008-2012 compared to period (1) 2003-2007. The position in period 2 was even weaker than the relative weak position in period 1. Brazil remained the strongest and the USA became strong.
    First step in using molecular data for microbial food safety risk assessment; hazard identification of Escherichia coli O157 : H7 by coupling genomic data with in vitro adherence to human epithelial cells
    Pielaat, Annemarie ; Boer, M.P. ; Wijnands, Lucas M. ; Hoek, A.H.A.M. van; Bouw, El ; Barker, G.C. ; Teunis, P.F.M. ; Aarts, Henk J.M. ; Franz, Eelco - \ 2015
    International Journal of Food Microbiology 213 (2015). - ISSN 0168-1605 - p. 130 - 138.
    GWAS - Microbiology - Risk assessment - SNP - STEC

    The potential for using whole genome sequencing (WGS) data in microbiological risk assessment (MRA) has been discussed on several occasions since the beginning of this century. Still, the proposed heuristic approaches have never been applied in a practical framework. This is due to the non-trivial problem of mapping microbial information consisting of thousands of loci onto a probabilistic scale for risks. The paradigm change for MRA involves translation of multidimensional microbial genotypic information to much reduced (integrated) phenotypic information and onwards to a single measure of human risk (i.e. probability of illness).In this paper a first approach in methodology development is described for the application of WGS data in MRA; this is supported by a practical example. That is, combining genetic data (single nucleotide polymorphisms; SNPs) for Shiga toxin-producing Escherichia coli (STEC) O157 with phenotypic data (in vitro adherence to epithelial cells as a proxy for virulence) leads to hazard identification in a Genome Wide Association Study (GWAS).This application revealed practical implications when using SNP data for MRA. These can be summarized by considering the following main issues: optimum sample size for valid inference on population level, correction for population structure, quantification and calibration of results, reproducibility of the analysis, links with epidemiological data, anchoring and integration of results into a systems biology approach for the translation of molecular studies to human health risk.Future developments in genetic data analysis for MRA should aim at resolving the mapping problem of processing genetic sequences to come to a quantitative description of risk. The development of a clustering scheme focusing on biologically relevant information of the microbe involved would be a useful approach in molecular data reduction for risk assessment.

    Economic impact of the Commission's 'opt-out' proposal on the use of approved GM crops : quick assessment of the medium-term economic consequences
    Hoste, R. ; Wagenberg, C.P.A. van; Wijnands, J.H.M. - \ 2015
    LEI Wageningen UR (Report / LEI Wageningen UR 2015-097) - ISBN 9789086157259 - 51
    transgenic plants - crops - genetic engineering - soyabeans - economic impact - agricultural sector - food industry - feed industry - european union - france - germany - poland - hungary - transgene planten - gewassen - genetische modificatie - sojabonen - economische impact - landbouwsector - voedselindustrie - veevoederindustrie - europese unie - frankrijk - duitsland - polen - hongarije
    The European Commission proposed the opportunity for individual EU Member States to restrict or prohibit the use of GMOs in food or feed on their territory (a national ‘opt-out’). The economic impact on individual sectors of the feed and food chain (the vegetable oil and meal industry, trade, animal feed industry, livestock sector) of a possible opt-out policy for soy by individual Member States has been assessed by LEI Wageningen UR.
    A single scenario was defined in which the four countries France, Germany, Poland and Hungary choose an ‘opt-out’ policy for soy. Consequences of this switch to non-GM soy and substitutes were assessed both quantitatively and qualitatively for feed prices, for production costs for animal production, for crushing industry and for trade, with a focus on the medium term
    Maatregelen emissiebeperking, effectiviteit en bereik via borgingsinstrumenten
    Kool, S.A.M. de; Wijnands, F.G. ; Gooijer, Y.M. ; Leendertse, P.C. ; Brinks, H. - \ 2015
    Praktijkonderzoek Plant & Omgeving, Business Unit Bloembollen, Boomkwekerij en Fruit (PPO publicatie 2015-665) - 67
    emissiereductie - emissie - chemische bestrijding - pesticiden - oppervlaktewater - grondwater - plantenkwekerijen - maïs - bloembollen - akkerbouw - tuinbouw - emission reduction - emission - chemical control - pesticides - surface water - groundwater - nurseries - maize - ornamental bulbs - arable farming - horticulture
    In 2012 is het project Water ABC gestart om waterkwaliteitsproblemen veroorzaakt door gewasbeschermingsmiddelen verder terug te dringen. In de volgende werkgebieden is in 2012-2013 gewerkt aan het vaststellen van de belangrijkste emissieroutes van de probleemstoffen en de selectie van mogelijke maatregelen en passende borgingsinstrumenten: Regio Boskoop, werkgebied Hoogheemraadschap van Rijnland: hier ligt het accent op de boomkwekerij. Zuidoost Nederland, werkgebied Waterschap Aa en Maas: accent op mais. Regio Noord en Zuid Holland, werkgebied van Hoogheemraadschap Rijnland en Hoogheemraadschap Hollands Noorderkwartier: accent bollenteelt. Friesland en Flevoland, werkgebied van Wetterskip Fryslân en Waterschap Zuiderzeeland: accent puntemissies akkerbouw. Brabant en Bommelerwaard, werkgebied van Brabant Water en Dunea: accent op kwetsbare gebieden (diverse sectoren: fruitteelt, akkerbouw), zowel oppervlaktewater als grondwater. Dit rapport documenteert de uitkomsten van het werk in de 5 gebieden. Achtereenvolgens komen per werkgebied aan de orde: de belangrijkste emissieroutes, de mogelijke maatregelen per route en de passende borgingsinstrumenten. Vervolgens wordt besproken hoe effectief het pakket geselecteerde maatregelen is in het beperken van de betreffende emissieroute. Daarna wordt ingezoomd op het bereik van de mogelijke borgingsinstrumenten. Zo ontstaat zicht op het werkelijke perspectief van beperking van emissie voor de verschillende werkgebieden en wordt duidelijk wat er eventueel nog gedaan moet worden.
    Gender dynamics in cashew and shea value chains from Ghana and Burkina Faso.
    Ingram, V.J. ; Yago-Quattara, E.L. ; Lartey, A. ; Mogre, D. ; Wijnands, Jo ; Berg, Jolanda van den - \ 2015
    LEI Wageningen UR - ISBN 9789086157198 - 55
    Measuring Competitiveness of Agro-food Industries – the Swiss Case
    Wijnands, J.H.M. ; Berkum, S. van; Verhoog, A.D. - \ 2015
    Paris : OECD - 59 p.
    Competitiveness of Swiss food industries
    Wijnands, J.H.M. ; Verhoog, A.D. ; Berkum, S. van - \ 2015
    In: Evaluation of Agricultural Policy Reforms In Switzerland - Working Party on Agricultural Policies and Markets / Vojtech, V., PARIS : OECD - p. 96 - 128.
    Integrated pest management adoption in the Netherlands: Experiences with pilot farm networks and stakeholder participation
    Wijnands, F.G. ; Brinks, H. ; Schoorlemmer, H. ; Bie, J. De - \ 2014
    In: Experiences with implementation, global overview / Peshin, R., Pimentel, D., Springer Netherlands - ISBN 9789400778016 - p. 513 - 554.
    Adoption - Farming systems research - Incentives - Integrated pest management - Integrated production - IPM - Pesticides - Pilot farms - Prototyping - Stakeholder management

    Integrated Pest Management in the Netherlands was developed in the 1980s and 1990s of the last century as a part of the wider concept of Integrated Farming. The potential proved to be very high in comparative farming systems studies proving that agrochemical inputs could be strongly reduced, notably pesticides. The Dutch government subsequently strongly advocated and supported the further development and implementation of Integrated Farming and Integrated Pest Management in practice. In the 1990s and 2000s, pilot farm networks were the cornerstone in the government strategy. The adoption of methods and techniques in practice however, progressed only slowly and unevenly. The support of the stakeholders in the agricultural community appeared to be essential to create sufficient momentum and ambition among farmers and contractors. The government's covenant on sustainable crop protection united in 2003 the stakeholders in a roundtable approach. At the same time, the national network project, Farming with Future,adopted a stakeholder management approach to mobilize the support and contribution of stakeholders in the development and subsequent introduction of Integrated Pest Management in practice. The results are promising.

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