Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    The impact of pectin supplementation on intestinal barrier function in healthy young adults and healthy elderly
    Wilms, Ellen ; Jonkers, Daisy M.A.E. ; Savelkoul, Huub F.J. ; Elizalde, Montserrat ; Tischmann, Lea ; Vos, Paul de; Masclee, Ad A.M. ; Troost, Freddy J. - \ 2019
    Nutrients 11 (2019)7. - ISSN 2072-6643
    Aging - Defense - Dietary fiber - Gastrointestinal - Intestinal permeability - Tight junctions - Tolerance

    Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the e ects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in di erent age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18–40 years) and 48 healthy elderly (65–75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not a ect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly (p ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly di erent between pectin versus placebo in both age groups (p ≥ 0.128). In both young adults and elderly, no di erences in transepithelial electrical resistance and fluorescein flux (p ≥ 0.164) and relative expression of genes analyzed (p ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not a ected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.

    Sugar Beet Pectin Supplementation Did Not Alter Profiles of Fecal Microbiota and Exhaled Breath in Healthy Young Adults and Healthy Elderly
    An, Ran ; Wilms, Ellen ; Smolinska, Agnieszka ; Hermes, Gerben D.A. ; Masclee, Ad A.M. ; Vos, Paul de; Schols, Henk A. ; Schooten, Frederik J. van; Smidt, Hauke ; Jonkers, Daisy M.A.E. ; Zoetendal, Erwin G. ; Troost, Freddy J. - \ 2019
    Nutrients 11 (2019)9. - ISSN 2072-6643
    aging - dietary fiber - elderly - exhaled air - microbiota - pectin - young adults

    Aging is accompanied with increased frailty and comorbidities, which is potentially associated with microbiome perturbations. Dietary fibers could contribute to healthy aging by beneficially impacting gut microbiota and metabolite profiles. We aimed to compare young adults with elderly and investigate the effect of pectin supplementation on fecal microbiota composition, short chain fatty acids (SCFAs), and exhaled volatile organic compounds (VOCs) while using a randomized, double-blind, placebo-controlled parallel design. Fifty-two young adults and 48 elderly consumed 15 g/day sugar beet pectin or maltodextrin for four weeks. Fecal and exhaled breath samples were collected before and after the intervention period. Fecal samples were used for microbiota profiling by 16S rRNA gene amplicon sequencing, and for analysis of SCFAs by gas chromatography (GC). Breath was used for VOC analysis by GC-tof-MS. Young adults and elderly showed similar fecal SCFA and exhaled VOC profiles. Additionally, fecal microbiota profiles were similar, with five genera significantly different in relative abundance. Pectin supplementation did not significantly alter fecal microbiota, SCFA or exhaled VOC profiles in elderly or young adults. In conclusion, aside from some minor differences in microbial composition, healthy elderly and young adults showed comparable fecal microbiota composition and activity, which were not altered by pectin supplementation.

    Effect of energy source in calf milk replacer on performance, digestibility, and gut permeability in rearing calves
    Amado, L. ; Berends, H. ; Leal, L.N. ; Wilms, J. ; Laar, H. Van; Gerrits, W.J.J. ; Martín-Tereso, J. - \ 2019
    Journal of Dairy Science 102 (2019)5. - ISSN 0022-0302 - p. 3994 - 4001.
    calf milk replacer - fat - gut permeability - lactose

    Current calf milk replacer (CMR) compositions significantly differ from whole milk in their levels of energy, protein, and minerals. Energy source is one of the major differences, as CMR contains high levels of lactose, whereas whole milk contains higher levels of fat. The aim of this study was to determine the effect of partially exchanging lactose for fat on performance, digestibility, and gut permeability in calves fed twice daily on a high feeding plane. Lactose and fat were exchanged in the CMR formulation on a weight–weight basis. The CMR were isonitrogenous but not isoenergetic. A total of 60 male Holstein-Friesian calves were assigned to 1 of 30 blocks based on serum IgG, body weight, and date of collection after birth. Within each block, calves were randomly assigned to 1 of 2 treatments: high fat and high lactose. The CMR was provided twice daily until 49 d of age, followed by a gradual weaning period of 14 d. Starter, straw, and water were available ad libitum throughout the complete study. Exchanging lactose for fat did not affect growth; intakes of starter, straw, water, crude protein, or total energy; or apparent total-tract digestibility of nutrients. Gastrointestinal permeability was assessed by measuring the recovery of lactulose and Cr in 24-h urine and the Cr concentration and lactulose:D-mannitol ratio in serum following an oral pulse dose. Urinary recoveries of Cr and lactulose were generally low in both treatments but were higher in calves fed the high-fat CMR. Accordingly, the serum lactulose:D-mannitol ratio and serum Cr concentrations were higher in calves fed the high-fat CMR. In wk 1 and during the weaning transition, calves fed the high-fat CMR had significantly fewer abnormal fecal scores. In conclusion, exchanging lactose for fat in the CMR did not affect growth performance, total feed intake, or nutrient digestibility. The high-fat CMR was associated with an increase in permeability markers but positively influenced fecal scores in calves.

    The effect of an encapsulated nutrient mixture on food intake and satiety : A double-blind randomized cross-over proof of concept study
    Alleleyn, Annick M.E. ; Avesaat, Mark van; Ripken, Dina ; Bleiel, Sinéad B. ; Keszthelyi, Daniel ; Wilms, Ellen ; Troost, Freddy J. ; Hendriks, Henk F.J. ; Masclee, Adrian A.M. - \ 2018
    Nutrients 10 (2018)11. - ISSN 2072-6643
    Carbohydrate - Distal release - Encapsulated nutrient mixture - Overweight - Protein - Satiety - Weight management

    Activation of the intestinal brake by infusing nutrients into the distal small intestine with catheters inhibits food intake and enhances satiety. Encapsulation of macronutrients, which protects against digestion in the proximal gastrointestinal tract, can be a non-invasive alternative to activate this brake. In this study, we investigate the effect of oral ingestion of an encapsulated casein and sucrose mixture (active) targeting the distal small intestine versus a control product designed to be released in the stomach on food intake, satiety, and plasma glucose concentrations. Fifty-nine volunteers received the active and control product on two separate test days. Food intake was determined during an ad libitum meal 90 min after ingestion of the test product. Visual analogue scale scores for satiety and blood samples for glucose analysis were collected at regular intervals. Ingestion of the active product decreased food intake compared to the control product (655 kcal compared with 699 kcal, respectively, p < 0.05). The area under the curve (AUC) for hunger was decreased (p < 0.05) and AUC for satiety was increased (p < 0.01) after ingestion of the active product compared to the control product. Ingestion of an encapsulated protein-carbohydrate mixture resulted in inhibition of food intake compared to a non-encapsulated control product.

    Age-dependent changes in GI physiology and microbiota : Time to reconsider?
    An, Ran ; Wilms, Ellen ; Masclee, Ad A.M. ; Smidt, Hauke ; Zoetendal, Erwin G. ; Jonkers, Daisy - \ 2018
    Gut 67 (2018)12. - ISSN 0017-5749 - p. 2213 - 2222.
    ageing - gastrointestinal physiology - intestinal bacteria

    Our life expectancy is increasing, leading to a rise in the ageing population. Ageing is associated with a decline in physiological function and adaptive capacity. Altered GI physiology can affect the amount and types of nutrients digested and absorbed as well as impact the intestinal microbiota. The intestinal microbiota is considered a key player in our health, and a variety of studies have reported that microbiota composition is changing during ageing. Since ageing is associated with a decline in GI function and adaptive capacity, it is crucial to obtain insights into this decline and how this is related to the intestinal microbiota in the elderly. Hence, in this review we focus on age-related changes in GI physiology and function, changes of the intestinal microbiota with ageing and frailty, how these are associated and how intestinal microbiota-targeted interventions may counteract these changes.

    PPS GROEN vergroent onbedekte aardbeiteelt
    Evenhuis, A. ; Wilms, J.A.M. - \ 2018
    Groenten & Fruit 2018 (2018)17. - ISSN 0925-9708 - p. 38 - 39.
    Prei op water : overzicht resultaten 2013 - 2017
    Os, E.A. van; Verhoeven, J. ; Wilms, J. - \ 2018
    Bleiswijk : Wageningen University & Research, BU Glastuinbouw (Rapport Wageningen Plant Research, Business unit Glastuinbouw WPR-764) - 42
    Within the 2nd programme Cultivation-out-of-the-soil for field crops (2014-2017) research f°Cussed onoptimisation of the cultivation, sowing on water, the use of a protective tunnel and upscaling. The robustnessof the growing system could be improved by these experiments. Economically the cost of traditional open fieldcropping is almost comparable with the cultivation on water (deep flow technique). In the plant raising stage adecrease in the growing period can be achieved to 60 instead of 84 days. Uniformity is important for a once overharvesting crop but still variation is big, grading of young plants helps a little. Oxygenation of larger ponds isimportant.
    Hydroponically grown leek : Slowly growing to maturity
    Os, E.A. van; Weel, P.A. van; Wilms, J.A.M. ; Bruins, M.A. ; Verhoeven, J. ; Wijk, K. van - \ 2017
    In: Proceedings of the ICESC2015. - International Society for Horticultural Science (Acta Horticulturae ) - ISBN 9789462611726 - p. 79 - 86.
    Deep flow technique - Floating panels - Nitrogen - Nitrogen efficiency - Water

    Leek traditionally grows in a sandy soil in the open field in The Netherlands. However, the vulnerability of sandy soils to leaching of nutrients to ground- and surface water is high. Therefore leek was chosen for experiments to be grown out of the soil in the open field. Investigations took place to certain aspects of system development which were reported in 2011. During the following years the research focused on commercializing the design (supporting tubes in floating panels in a DFT system), upscaling the system to several ponds of 200 m2, marketing the product, solving specific problems which appear during those experiments (oxygen content of the nutrient solution, continuous circulation, virus symptoms) and economic aspects. The semi-commercial system developed was based on various trials at which a plant density of more than 70 plants m-2 is needed to achieve plants of more than 200 g which are thicker than 2.0 cm. Hydroponically grown leek should be graded before planting on the panels. Fast growing cultivars should be chosen year round. Uniform planting material is needed for a uniform, single date, harvest. The age of the plant is of less importance for the quality of the harvested product. This gives flexibility in the planting date which often depends on the harvesting date of the former crop and, consequently the weather before. Besides the plants should be >20 g to be planted in the 20 cm long tubes, otherwise the growth is delayed (no catchment of light inside the tube). The cultivation period was similar to earlier trials (50-75 d). The taste of hydroponically grown leek was comparable with leeks raised in soil. During cultivation, symptoms of the yellow stripe virus were seen more frequent compared to soil grown leek. Reuse of the nutrient solution during 4 crops a year saves about 50% of the fertilizers required. Another aspect of importance in The Netherlands is the surplus of rainfall (800 mm average year-1) during the growing season compared to the water uptake (300-500 mm). Adaptation of the system or discharge strategy was investigated Hydroponically grown leek slowly grows to a mature cultivation system by solving the topics mentioned-above, therefor it can be used by commercial growers now. The importance of a slow upscale was proven by the appearance of several unforeseen aspects (oxygen, rainfall, duration of irrigation), while in marketing a first step is taken to bring hydroponically grown leek as a distinguishable product.

    Afploegen aspergeruggen in nieuw perspectief
    Wilms, Jos - \ 2017
    Building with Nature - an integrated approach for coastal zone solutions using natural, socio-economic and institutional processes
    Wilms, T. ; Goot, F. van der; Debrot, A.O. - \ 2017
    - 7 p.
    integrated coastal zone management - system understanding - non-traditional solutions mangrove restoration - socio-economics - muddy coast
    This paper presents Building with Nature as a viable alternative to the traditional engineering approach, making the services that nature provides an integral part of the design of hydraulic infrastructure, thereby creating benefits for nature and society. In it we describe the necessary steps with which to implement a
    Building with Nature approach. Our case study in Demak, Central Java, Indonesia is used for examples to illustrate this approach and the lessons learnt on benefits and challenges. The location in Demak concerns a tropical muddy mangrove coast. During the last decades, in several areas the coastline has retreated hundreds of meters up to several kilometres, while in other parts of the project area the threat of erosion and flooding by the sea and decline of aquaculture productivity continue to worsen. In 2015, a pilot project to restore the natural coastal mangrove forest was started. The first step was to
    establish a good understanding of the complex natural and local socio-economic environments. Based on this system understanding, we then chose for non-traditional solutions using temporary permeable structures made from local material to create wave-sheltered areas that stimulate the settlement of sediment and create a habitat favourable to mangrove recolonisation. Once the mangrove forest is fully-grown it will provide protection against waves. It will also provide other ecosystem services like food provisioning, tourism, nursery habitat for fishery production and CO2-storage. A long-term sustainable solution requires the integration of these technical measures into the local socio-economic and governmental context. To support a smooth transition towards sustainable practices, local communities are simultaneously trained in
    sustainable methods to improve the productivity of their aquaculture ponds. This is done using “coastal field schools” as modelled from the “farmer field school” methodology developed by the FAO in 1989 for rural development. The approach is embedded in the village regulations. Sustainability in this rural area is created by closely linking safety and livelihood. The key lessons learnt from this project are that a combination of a thorough understanding of the biophysical, socio-economic and governmental system and early stakeholder involvement results in higher vital benefits, reduces costs and provides the setting for sustainable design solutions. It requires a learning and adaptive planning cycle from all participants as this approach exemplifies a “learning by doing” approach.
    Effects of supplementation of the synbiotic Ecologic® 825/FOS P6 on intestinal barrier function in healthy humans : A randomized controlled trial
    Wilms, E. ; Gerritsen, J. ; Smidt, H. ; Besseling-van der Vaart, I. ; Rijkers, G.T. ; Garcia Fuentes, A.R. ; Masclee, A.A.M. ; Troost, F.J. - \ 2016
    PLoS ONE 11 (2016)12. - ISSN 1932-6203

    Background and Aims: Probiotics, prebiotics and synbiotics have been suggested as dietary strategies to improve intestinal barrier function. This study aimed to assess the effect of two weeks synbiotic supplementation on intestinal permeability under basal and stressed conditions. Secondary aims were the assessment of two weeks synbiotic supplementation on systemic immune function and gastrointestinal symptoms including defecation pattern. Design: Twenty healthy adults completed a double-blind, controlled, randomized, parallel design study. Intervention: Groups either received synbiotic (1.5 × 1010 CFU Ecologic® 825 + 10 g fructo-oligosaccharides (FOS P6) per day) or control supplements for two weeks. Outcomes: Intestinal segment specific permeability was assessed non-invasively by oral administration of multiple sugar probes and, subsequently, assessing the excretion of these probes in urine. This test was conducted at baseline and at the end of intervention, in the absence and in the presence of an indomethacin challenge. Indomethacin was applied to induce a compromised gut state. Plasma zonulin, cytokines and chemokines were measured at baseline and at the end of intervention. Gastrointestinal symptoms and stool frequency were recorded at baseline and daily during intervention. Results: Significantly more male subjects were in the synbiotic group compared to the control group (P = 0.025). Indomethacin significantly increased urinary lactulose/rhamnose ratio versus without indomethacin, both in the control group (P = 0.005) and in the synbiotic group (P = 0.017). Urinary sugar recoveries and ratios, plasma levels of zonulin, cytokines and chemokines, and gastrointestinal symptom scores were not significantly different after control or synbiotic intervention. Stool frequency within the synbiotic group was significantly increased during synbiotic intervention compared to baseline (P = 0.039) and higher compared to control intervention (P = 0.045). Conclusion: Two weeks Ecologic® 825/FOS P6 supplementation increased stool frequency, but did not affect intestinal permeability neither under basal nor under indomethacin-induced stressed conditions, immune function or gastrointestinal symptoms in healthy adults.

    Building with Nature Indonesia: securing eroding delta coastlines : Design and Engineering Plan
    Tonneijck, F.H. ; Winterwerp, H. ; Weesenbeeck, Bregje van; Bosma, R.H. ; Debrot, A.O. ; Rusila Noor, Yus ; Wilms, T. - \ 2015
    Ecoshape - 65
    Echte meeldauw in aardbei
    Evenhuis, A. ; Topper, C.G. ; Wilms, J.A.M. - \ 2014
    aardbeien - vollegrondsteelt - gewasbescherming - meeldauw - sphaerotheca - schimmelbestrijding - ziektebestrijdende teeltmaatregelen - strawberries - outdoor cropping - plant protection - mildews - sphaerotheca - fungus control - cultural control
    Echte meeldauw wordt veroorzaakt door Sphaerotheca macularis. De schimmel kent weinig andere waardplanten. Biologie van de schimmel, weersomstandigheden en aardbeiplant bepalen kans op infectie. Hierop zijn waarschuwingssytemen gebaseerd.
    Sporulatie en beheersing echte meeldauw in aardbei : bouwstenen voor beslissing ondersteunend systeem (BOS) voor de beheersing van meeldauw in aardbei
    Evenhuis, A. ; Topper, C.G. ; Wilms, J.A.M. - \ 2014
    Wageningen : Praktijkonderzoek Plant & Omgeving, Business Unit AGV - 47
    aardbeien - fragaria ananassa - gewasbescherming - meeldauw - beslissingsondersteunende systemen - vruchtrot - teeltsystemen - alternatieve methoden - fungiciden - sporulatie - biologische processen - strawberries - fragaria ananassa - plant protection - mildews - decision support systems - fruit rots - cropping systems - alternative methods - fungicides - sporulation - biological processes
    Echte meeldauw in aardbei kan zowel het blad als de vrucht aantasten. In de praktijk wordt de schimmel bestreden door regelmatige inzet van gewasbeschermingsmiddelen. Het is gewenst om die inzet te beperken tot het noodzakelijke. Dit is zowel economisch en milieutechnisch aantrekkelijk. In het algemeen kunnen waarschuwingssystemen helpen om het tijdstip van de inzet van gewasbeschermingsmiddelen te optimaliseren. Voor echte meeldauw zijn er een aantal beslissingsondersteunende systemen (BOS) op de markt (PlantPLus van Dacom Plant service en Aardbei bericht van Agrovision). In de teelt van aardbei wordt een advies gegeven voor vruchtrot en een advies voor meeldauw. Ervaring leert dat het advies voor meeldauw toch lastig is door wisselende weersomstandigheden en daar komt nog bij de aardbeien geteeld op stellingen, al of niet met regenkapje, te maken hebben met een ander microklimaat dan aardbeien geteeld in de vollegrond. Voor meeldauw geldt dan ook nog dat de biologie van deze schimmel minder goed bekend is dan die van Botrytis cinerea (vruchtrot). Het project is er op gericht om een de biologie van echte meeldauw in aardbei verder te ontrafelen. Deze kennis kan gebruikt worden om de bestrijdingsstrategie voor echte meeldauw in aardbei te ontwikkelen, gebaseerd op de biologie van de schimmel, de weersomstandigheden en de vitaliteit van het gewas. Veel gegevens over kieming, infectie en kolonisatie van aardbeiblad zijn beschreven in de literatuur.
    Vissers betrekken in onderzoek en beheer: in de toekomst 9 miljard monden te voeden (interview met Marloes Kraan en Floor Quirijns)
    Kraan, M.L. ; Quirijns, F.J. - \ 2014
    Visserijnieuws 34 (2014)14. - ISSN 1380-5061 - p. 4 - 4.
    visserij - vis - vissers - duurzaamheid (sustainability) - wetenschappelijk onderzoek - aquatische ecosystemen - visserijbeheer - ecosystemen - fisheries - fish - fishermen - sustainability - scientific research - aquatic ecosystems - fishery management - ecosystems
    Hoe betrekken we vissers bij onderzoek en beheer? Dat was het hoofdthema van de conferentie 'Fisheries Dependent information' vorige maand in Rome. Maar ook onderwerpen als de aanlandplicht en monitoring met camera's kwamen voorbij. Aanweizig op de conferentie was een flinke Nederlandse delegatie. Marloes Kraan en Floor Quirijns van IMARES Wageningen UR en Inger Wilms (CVO) doen kort verslag.
    Onderzoek naar verband tussen grond- en plantsap-gehalten en bewaarresultaat Chinese kool 2013/14
    Wijk, K. van; Wilms, J.A.M. ; Verstegen, H.A.G. - \ 2014
    Lelystand : Praktijkonderzoek Plant & Omgeving, Business Unit Akkerbouw, Groene Ruimte en Vollegrondsgroenten (PPO 613) - 43
    chinese koolsoorten - opslag - houdbaarheid (kwaliteit) - opslagkwaliteit - bemesting - stikstof - meting - vollegrondsgroenten - bladgroenten - chinese cabbages - storage - keeping quality - storage quality - fertilizer application - nitrogen - measurement - field vegetables - leafy vegetables
    Chinese kooltelers willen graag productverlies bij bewaarkool beperken en de afzetperiode verlengen tot eind maart. Het merendeel van de partijen wordt nu in januari/februari geruimd. Het grootste verlies wordt veroorzaakt door vochtverlies tijdens de bewaring en door rot en stip-aantasting. In het eerste onderzoekseizoen 2012/13 is, na literatuurstudie, gestart met onderzoek naar: a) N-bemestingswijzen, planttijdstippen en plantsap-analyses, b) Controlled Atmosphere (CA)-bewaring in hoezen en c) praktijktoetsing van ethyleengehalten in bewaarcellen. CA-bewaring bleek te duur voor de kleine kwaliteitsverbetering die het opleverde en is niet voortgezet. De ethyleengehalten in praktijkcellen bleken laag en niet schadelijk. Daarom is in 2013/2014 is het bewaaronderzoek Chinese kool voortgezet met a) N- bemestingswijzen en b) bewaring van 9 praktijkherkomsten. Beide in combinatie met bodemvruchtbaarheids- en plantsap-analyses. Het doel was via deze analyses parameters te vinden, waarmee te sturen is op betere bewaring van Chinese kool (vooral door beperking van stipaantasting) dan wel de bewaarbaarheid van partijen te voorspellen is, zodat daar het uithaaltijdstip van koolpartijen op afgestemd kan worden.
    Effect bemesting op ziekteontwikkeling in stamslabonen industrieteelt
    Evenhuis, B. ; Verstegen, H.A.G. ; Wilms, J.A.M. ; Topper, C.G. - \ 2014
    Wageningen : PPO AGV - 22
    groenteteelt - veldgewassen - phaseolus vulgaris - peulvruchten (groente) - industriële gewassen - bemesting - stikstofmeststoffen - overbemesting - oogstverliezen - opbrengst - relatie tussen groei en oogst - ziektebestrijdende teeltmaatregelen - schimmelziekten - sclerotinia - botrytis - gewasbescherming - vegetable growing - field crops - phaseolus vulgaris - vegetable legumes - industrial crops - fertilizer application - nitrogen fertilizers - top dressings - yield losses - outturn - growth yield relationship - cultural control - fungal diseases - sclerotinia - botrytis - plant protection
    In de teelt van stamslabonen voor de industrie worden vaak problemen ondervonden met ziektes zoals Sclerotinia en Botrytis. Deze ziektes worden geassocieerd met (over)bemesting. De gebruiksnorm voor stikstofgift was in 2012 en 2013, 110 kg N/ha respectievelijk 120 kg N/ha, op zand- en kleigrond. Werd er niet bemest dan leidde dit tot een significante opbrengstverlaging. Een overbemesting tot 150% van de gebruiksnorm na aftrek van N-min gaf eveneens een opbrengstverlaging te zien. Bemesting trappen variërend van 50 tot 125% van de normbemesting lieten geen significant verschil in opbrengst zien. Een evenwichtig teeltadvies waarbij het bemestingsniveau maximaal bijdraagt aan plantweerstand en productkwaliteit is belangrijk.
    Monitoring Aspergevliegen 2013 Gewastellingen en lijmstokvangsten van aspergevliegen in veertien aspergevelden
    Rozen, K. van; Wilms, J.A.M. - \ 2014
    Lelystad : PPO AGV - 55
    asparagus - stengelgroenten - plagen - veldgewassen - vollegrondsgroenten - plantenplagen - geïntegreerde plagenbestrijding - ziektebestrijdende teeltmaatregelen - gewasbescherming - veldproeven - boorders (insecten) - insectenplagen - plagenbestrijding - asparagus - stem vegetables - pests - field crops - field vegetables - plant pests - integrated pest management - cultural control - plant protection - field tests - boring insects - insect pests - pest control
    Monitoringssysteem voor aspergevliegen ontwikkelen met bijbehorende advisering. De resultaten in 2012 en 2013 van 26 gemonitorde percelen geven voldoende aanleiding tot een gerichtere aanpak van aspergevliegen. Dit betekent ook vaak dat niet hoeft worden ingegrepen. In 2013 is een schadedrempel geformuleerd op basis van de uitkomsten; bestrijding uitvoeren nadat gemiddeld 10 aspergevliegen/val/week zijn geteld. Op dit moment wordt aanbevolen om deze schadedrempel in de praktijk te valideren.
    Stikstof hand in hand met zwarte stip : bewaarproef PPO Vredepeel
    Verstegen, S. ; Verstegen, H.A.G. ; Wijk, C.A.P. van; Wilms, J.A.M. - \ 2014
    Groenten & Fruit 2014 (2014)8. - ISSN 0925-9708 - p. 31 - 31.
    Can transcriptomics provide insight into the underlying chemopreventive mechanisms of complex mixtures of phytochemicals in humans?
    Breda, S.G. van; Wilms, L.C. ; Gaj, S. ; Briedé, J.J. ; Helsper, J.P.F.G. ; Kleinjans, J.C. ; Kok, T.M. de - \ 2014
    Antioxidants and redox signaling 20 (2014)14. - ISSN 1523-0864 - p. 2107 - 2113.
    Blueberries contain relatively large amounts of different phytochemicals which are suggested to have chemopreventive properties, but little information is available on the underlying molecular modes of action. This study investigates whole genome gene expression changes in lymphocytes of 143 humans after a four-week blueberry-apple juice dietary intervention. Differentially expressed genes and genes correlating with the extent of antioxidant protection were identified in 4 subgroups. The magnitude of the preventive effect after the intervention differed between these four subgroups. Furthermore, subjects in two groups carried genetic polymorphisms that were previously found to influence the chemopreventive response. Pathway analysis of the identified genes showed strong but complex gene expression changes in pathways signaling for apoptosis, immune response, cell adhesion, and lipid metabolism. These pathways indicate increased apoptosis, upgraded growth control, induced immunity, reduced platelet aggregation and activation, blood glucose homeostasis, and regulation of fatty acid metabolism. Based on these observations, we hypothesize that combining transcriptomic data with phenotypic markers of oxidative stress may provide insight into the relevant cellular processes and genetic pathways which contribute to the antioxidant response of complex mixtures of phytochemicals, such as found in blueberry-apple juice
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