Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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    Trust in farm data sharing: reflections on the EU code of conduct for agricultural data sharing
    Burg, Simone van der; Wiseman, Leanne ; Krkeljas, Jovana - \ 2020
    Ethics and Information Technology (2020). - ISSN 1388-1957
    Digital farming - Ethics - EU code of conduct - GDPR - Informed consent - Trust

    Digital farming technologies promise to help farmers make well-informed decisions that improve the quality and quantity of their production, with less labour and less impact on the environment. This future, however, can only become a reality if farmers are willing to share their data with agribusinesses that develop digital technologies. To foster trust in data sharing, in Europe the EU Code of Conduct for agricultural data sharing by contractual agreement was launched in 2018 which encourages transparency about data use. This article looks at the EU Code through the lens of literature on trust and contract agreements. We agree with the makers of the EU Code that a contract can make an important contribution to trust relationships as it is needed to mitigate the detrimental effects of power relationships between experts and non-experts. Building on Onora O’Neills perspective to trust, however, we argued that a contract can only be successful in fostering trust when (a) information is comprehended by the more vulnerable party in this relationship who has to sign the contract, (b) the more powerful partner takes responsibility to provide that information, and (c) information is tailored to the information needs of the party signing the contract, even when data are re-used over a longer period. In addition, we think that differences between trust relationships and relationships of accountability, give reason to add to informed consent other more substantive ethical components in a more encompassing code of conduct.

    Mode of action based risk assessment of the botanical food-borne alkenylbenzenesapiol and myristicin
    Alajlouni, Abdul - \ 2017
    Wageningen University. Promotor(en): I.M.C.M. Rietjens, co-promotor(en): J.J.M. Vervoort; S. Wesseling. - Wageningen : Wageningen University - ISBN 9789463434584 - 212

    Alkenylbenzenes including estragole, methyleugenol, safrole, elemicin, apiol, and myristicin are naturally occurring in many herbs such as parsley, dill, basil, tarragon, fennel and nutmeg (Kreydiyyeh and Usta, 2002, Smith et al., 2002, Semenov et al., 2007). Estragole, methyleugenol and safrole are genotoxic and carcinogenic in rodent bioassays inducing liver tumors (Boberg et al., 1986, Boberg et al., 1983, Drinkwater et al., 1976, Miller et al., 1983, Swanson et al., 1981, Wiseman et al., 1985, Wiseman et al., 1987, Wislocki et al., 1977). Because of that, the use of methyleugenol, safrole and estragole as pure substances in foodstuff has been prohibited in the EU from September 2008 onwards (European Commission (EC), 2008). For apiol and myristicin data for their risk assessment are limited and more research is needed to support the evaluation of the risk resulting from consumption of products containing these compounds (WHO, 2009). The aim of the current thesis was to perform a mode of action based risk assessment of exposure to low doses of apiol and myristicin by using physiologically based kinetic (PBK) modelling based read-across from other alkenylbenzenes and to use the results obtained for risk assessment of consumption of plant food supplements (PFS) and other botanical products containing parsley and dill.

    Chapter 1 provides general background information to alkenylbenzenes especially apiol and myristicin, a description of the chemical, metabolic and toxicity characteristics of apiol and myristicin and other structurally related alkenylbenzenes, a brief outline of the method used for their risk assessment and a short introduction to PBK modelling. Besides that, Chapter 1 include the aim of the current thesis. In Chapter 2 and Chapter 3, PBK models for respectively apiol and myristicin in male rat and human were defined, enabling prediction of dose-dependent effects in bioactivation and detoxification of these alkenylbenzenes. The PBK model based predictions were subsequently compared to those for safrole enabling estimation of a BMDL10 for apiol and myristicin from read-across from the BMDL10 available for safrole, thereby enabling risk assessment of current dietary exposure to apiol. In Chapter 4 and 5, the risk assessment of exposure to apiol and related alkenylbenzenes through drinking of parsley and dill based herbal teas and consumption of parsley and dill containing PFS was performed using the BMDL10 values derived in Chapter 2 and 3. The results showed that consumption of parsley and dill based herbal teas and PFS would be a priority for risk management if consumed for longer periods of time. Chapter 6 includes a general discussion of the thesis results obtained and the future perspectives that describe the needs to further research, based on alternatives for animals testing, to improve the risk assessment approaches for different botanical preparations.

    Altogether, the results obtained through different thesis chapters show that integration of different approaches provides the basis for a mode of action and PBK modelling based read-across from compounds for which tumor data are available to related compounds for which such data are lacking. This can contribute to the development of alternatives for animal testing and will facilitate the risk assessment of compounds for which in vivo toxicity studies on tumor formation data are unavailable.

    Strengthening the evidence base for nutrition and cancer in low and middle income countries
    Romieu, Isabelle ; Margetts, Barrie ; Barquera, Simón ; Silva Gomes, Fabio da; Gunter, Marc ; Hwalla, Nahla ; Kampman, Ellen ; Leitzmann, Michael ; Potischman, Nancy ; Slimani, Nadia ; Vorster, Este ; Willett, Walter C. ; Winichagoon, Pattanee ; Wiseman, Martin - \ 2016
    Journal of Global Health 6 (2016)2. - ISSN 2047-2978
    Nitrogen efficiency and amino acid requirements in dairy cattle
    Vuuren, A.M. van; Dijkstra, J. ; Reynolds, C.K. ; Lemosquet, S. - \ 2015
    In: Recent Advances in Animal Nutrition 2014 / Garnsworthy, P.C, Wiseman, J., Packington, UK : Context Products Ltd - ISBN 9781899043699 - p. 49 - 63.
    Considerations for feeding starch to high yielding dairy cows
    Reynolds, C.K. ; Humphries, D.J. ; Vuuren, A.M. van; Dijkstra, J. ; Bannink, A. - \ 2015
    In: Recent Advances in Animal Nutrition 2014 / Garnsworthy, P.C, Wiseman, J., Packington, UK : Context Products Ltd - ISBN 9781899043699 - p. 27 - 47.
    Matrix modulation of the toxicity of alkenylbenzenes, studied by an integrated approach using in vitro, in vivo, and physiologically based biokinetic models
    Al-Husainy, W.A.A.M. - \ 2013
    Wageningen University. Promotor(en): Ivonne Rietjens; Peter van Bladeren, co-promotor(en): Ans Punt. - Wageningen : Wageningen UR - ISBN 9789461738066 - 199
    methyleugenol - toxiciteit - keukenkruiden - flavonoïden - methyl eugenol - toxicity - culinary herbs - flavonoids

    Alkenylbenzenes such as estragole and methyleugenol are common components of spices and herbs such as tarragon, basil, fennel, mace, allspice, star anise and anise and their essential oils (Smithet al., 2002). There is an interest in the safety evaluation of alkenylbenzenes because these compounds can induce hepatic tumours in rodents when dosed orally at high dose levels (Milleret al., 1983; NTP, 2000). Based on the rodent studies with estragole, methyleugenoland structurally related alkenylbenzenes like safrole the hepatocarcinogenicity of alkenylbenzenes is ascribed to their bioactivation by cytochrome P450 enzymes leading to the formation of the proximate carcinogenen, the 1′-hydroxy metabolite, which is further bioactivated to the ultimate carcinogenen, the 1′-sulfooxy metabolite (Milleret al., 1983; Phillipset al., 1984; Randerathet al., 1984; Smithet al., 2010). The 1′-sulfooxy metabolite is unstable and binds via a presumed reactive carbocation intermediate covalently to different endogenous nucleophiles including DNA (Phillipset al., 1981; Boberget al., 1983; Milleret al., 1983; Phillipset al., 1984; Randerathet al., 1984; Fennellet al., 1985; Wisemanet al., 1987; Smithet al., 2002).

    Because of their genotoxicity and carcinogenicity, the addition of estragole and methyleugenolas pure substances to foodstuffs has been prohibited within the European Union since September 2008 (European Commission, 2008). In 2008, the Joint FAO/WHO Expert Committee on Food Additives (JECFA) re-evaluated the safety of alkenylbenzenes and indicated that although evidence of carcinogenicity to rodents given high doses of alkenylbenzenes exists, further research is needed to assess the potential risk to human health at relevant dietary exposure levels (JECFA, 2008).

    A significant difficulty in evaluating the toxicological data for alkenylbenzenes is that human exposure to these substances results from exposure to a complex mixture of food, spice, and spice oil constituents which may influence the biochemical fate and toxicological risk of the alkenylbenzenes. In this regard, it was shown that a methanolic extract of basil inhibited the formation of estragole DNA adducts in human HepG2 cells exposed to the proximate carcinogen 1′-hydroxyestragole (Jeurissenet al., 2008). This inhibition occurred at the level of sulfotransferase (SULT)-mediated bioactivation of 1′-hydroxyestragole into 1′-sulfooxyestragole (Jeurissenet al., 2008).

    The objective of this PhD research was to study the inhibitory action of components in alkenylbenzene-containing herbs and spices on SULT-mediated alkenylbenzene DNA adduct formation and the consequences of this combination effect for risk assessment using estragole and methyleugenol as the model alkenylbenzenes. To achieve this objective, an integrated approach of in vitro, in vivo and physiologically based biokinetic (PBBK) models was applied to investigate how the SULT inhibition influences the bioactivation and thus potentially also the toxicity and risk assessment of estragole and methyleugenol.

    Chapter 1of the thesis presents an introduction to the bioactivation, detoxification, genotoxicity and carcinogenicity of the alkenylbenzenes estragole and methyleugenol as well as a short introduction to PBBK modeling and the state-of-the-art knowledge on risk assessment strategies and regulatory status for alkenylbenzenes.

    Chapter 2of the thesis identifies nevadensin as a basil constituent able to inhibit SULT-mediated DNA adduct formation in rat hepatocytes exposed to the proximate carcinogen 1′-hydroxyestragole and nevadensin. The type of inhibition by nevadensin was shown to be non-competitive with an inhibition constant (Ki) of 4 nM. Furthermore, nevadensin up to 20 μM did not inhibit 1′-hydroxyestragole detoxification by glucuronidation and oxidation. The inhibition of SULT by nevadensin was incorporated into the PBBK models describing bioactivation and detoxification of estragole in male rat and human. The models thus obtained predict that co-administration of estragole at a level inducing hepatic tumours in vivo (50 mg/kg bw) with nevadensin at a molar ratio to estragole representing the molar ratio of their occurrence in basil, results in more than 83% inhibition of the formation of the carcinogenic metabolite, 1ʹ-sulfooxyestragole, inthe liver of male rat and human even at 1% uptake of nevadensin.

    To extend the work to other alkenylbenzene-containing herbs and spices than basil chapter 3 presents data showing that methanolic extracts from different alkenylbenzene-containing herbs and spices such as nutmeg, mace, anise and others are able to inhibit the SULT enzyme activity. Flavonoids including nevadensin, quercetin, kaempferol, myricetin, luteolin and apigenin were the major constituents responsible for this inhibition of SULT activity with Kivalues in the nano to sub-micromolar range. Also, the various flavonoids individually or in mixtures were able to inhibit estragole DNA adduct formation in human HepG2 cells exposed to the proximate carcinogen 1ʹ-hydroxyestragole, and to shift metabolism in favour of detoxification (e.g. glucuronidation) at the cost of bioactivation (e.g. sulfonation).

    In a next step, the kinetics for SULT inhibition were incorporated in PBBK models for estragole in rat and human to predict the effect of co-exposure to estragole and (mixtures of) the different flavonoids on the bioactivation in vivo. The PBBK-model-based predictions indicate that the reduction of estragole bioactivation in rat and human by co-administration of the flavonoids is dependent on whether the intracellular liver concentrations of the flavonoids can reach their Ki values. Finally, we concluded that it is expected that this is most easily achieved for nevadensin which has a Kivalue in the nanomolar range and is, due to its methylation, more metabolically stable and bioavailable than the other flavonoids.

    Chapter 4of the thesis investigates whether the previous observation that nevadensin is able to inhibit SULT-mediated estragole DNA adduct formation in primary rat hepatocytes could be validated in vivo. Moreover, the previously developed PBBK models to study this inhibition in rat and in human liver was refined by including a sub-model describing nevadensin kinetics. Nevadensin resulted in a significant reduction in the levels of estragole DNA adducts formed in the liver of Sprague–Dawley rats orally dosed with estragole and nevadensin simultaneously at a ratio reflecting their presence in basil. Moreover, the refined PBBK model predicted the formation of estragole DNA adducts in the liver of rat with less than 2-fold difference compared to in vivo data and suggests more potent inhibition in the liver of human compared to rat due to less efficient metabolism of nevadensin in human liver and intestine.

    Also, an updated risk assessment for estragole was presented taking into account the matrix effect and this revealed that the BMDL10 and the resulting MOE for estragole increase substantially when they would be derived from rodent bioassays in which the animals would be exposed to estragole in the presence of nevadensin instead of to pure estragole.

    To extend the work to other alkenylbenzenes than estragole chapter 5 of the thesis investigates the potential of nevadensin to inhibit the SULT-mediated bioactivation and subsequent DNA adduct formation of methyleugenolusing human HepG2 cells as an in vitro model. Nevadensin was able to inhibit SULT-mediated DNA adduct formation in HepG2 cells exposed to the proximate carcinogen 1′-hydroxymethyleugenol in the presence of nevadensin.To investigate possible in vivo implications for SULT inhibition by nevadensin on methyleugenolbioactivation, the rat PBBK model developed in our previous work to describe the dose-dependent bioactivation and detoxification of methyleugenolin male rat was combined with the recently developed PBBK model describing the dose-dependent kinetics of nevadensin in male rat. Similar to what was presented for estragole in chapter 4, chapter 5 presents an updated risk assessment for methyleugenoltaking the matrix effect into account. This revealed that the BMDL10 and the resulting MOE for methyleugenolincrease substantially when they would be derived from rodent bioassays in which the animals would be exposed to methyleugenolin the presence of nevadensin instead of to pure methyleugenol.

    In a next step, we aimed at moving one step forward towards endpoints that are closer to initiation of carcinogenesis than DNA adduct formation, namely, formation of hepatocellular altered foci (HAF). Chapter 6 presents data showing that the potent in vivo inhibitory activity of nevadensin on SULT enzyme activity and on alkenylbenzene DNA adduct formation is accompanied by a potent in vivo reduction in early markers of carcinogenesis such as HAF. This also suggests that a reduction in the incidence of hepatocarcinogenicity is expected in liver of rodents when alkenylbenzenes would be dosed simultaneously with nevadensin.

    Chapter 7presents a discussion on the in vitro and in vivo activity of dietary SULT inhibitors and their potential in reducing the cancer risk associated with alkenylbenzene consumption. This chapter also presents some future perspectives based on the major issues raised by our research.

    Altogether, the results of the present thesis indicate that the likelihood of bioactivation and subsequent adverse effects may be lower when alkenylbenzenes are consumed in a matrix containing SULT inhibitors such as nevadensincompared to experiments using pure alkenylbenzenes as single compounds. Also,the consequences of the in vivo matrix effect were shown to be significant when estragole or methyleugenolwas tested in rodent bioassays in the presence of nevadensin at ratios detected in basil, thereby likely increasing BMDL10 and resulting MOE values substantially in a subsequent risk assessment. However, the results also indicate that matrix effects may be lower at daily human dietary exposure levels of estragole or methyleugenoland nevadensin resulting from basil consumption. Also, matrix effects seem to be limited in the presence of other SULT inhibiting dietary flavonoids even at high exposure levels of these flavonoids coming from supplements. This indicates that the importance of a matrix effect for risk assessment of individual compounds requires analysis of dose dependent effects on the interactions detected, an objective that can be achieved by using PBBK modeling.

    Overall, the present study provides an example of an approach that can be used to characterise dose- species- and inter-individual differences as well as matrix effects in the risk assessment of food-borne toxicants present (e.g. alkenylbenzenes). In this approach the most important toxicokinetic interactions are addressed using an integrated strategy of in vitro, in vivo and PBBK modeling approaches.

    Intermittent suckling: tackling lactational anoestrus and alleviating weaning risks for piglets
    Langendijk, P. ; Berkeveld, M. ; Gerritsen, R. ; Soede, N.M. ; Kemp, B. - \ 2007
    In: Paradigms in pig science / Wiseman, J., Varley, M.A., McOrist, S., Kemp, B., Nottingham : Nottingham University Press - ISBN 9781904761563 - p. 359 - 383.
    Influence of nutritional factors on placental growth and piglet imprinting
    Hazeleger, W. ; Ramaekers, P. ; Smits, C. ; Kemp, B. - \ 2007
    In: Paradigms in pig science / Wiseman, J., Varley, M.A., McOrist, S., Kemp, B., Nottingham : Nottingham University Press - ISBN 9781904761563 - p. 309 - 327.
    Paradigms in pig science
    Wiseman, J. ; Varley, M.A. ; McOrist, S. ; Kemp, B. - \ 2007
    Nottingham : Nottingham University Press (Proceedings of Easter Schools in Agricultural Science ) - ISBN 9781904761563 - 538
    varkens - varkenshouderij - dierlijke productie - dierhouderij - varkensvoeding - diervoeding - dierveredeling - dierfysiologie - diergezondheid - varkensvlees - varkensfokkerij - pigs - pig farming - animal production - animal husbandry - pig feeding - animal nutrition - animal breeding - animal physiology - animal health - pigmeat - pig breeding
    Impact of liberalisation of food and land markets on agrarian land use in the EU
    Vereijken, P.H. ; Hermans, C.M.L. ; Naeff, H.S.D. - \ 2005
    In: Yields of farmed species: constraints and opportunities in the 21st century / Bradley, R.S., Wiseman, J., Nottingham, UK : Nottingham University Press - ISBN 9781904761235 - p. 15 - 29.
    Insemination strategies in swine: physiological backgrounds and practical consequences
    Soede, N.M. ; Langendijk, P. ; Kemp, B. - \ 2003
    In: Perspectives in Pig Science / Wiseman, J., Varley, M.A., Kemp, B., Nottingham UK : Nottingham University Press - ISBN 9781897676196 - p. 257 - 278.
    varkens - kunstmatige inseminatie - dierfysiologie - voortplanting - fysiologie - pigs - artificial insemination - animal physiology - reproduction - physiology
    Perspectives in Pig Science
    Wiseman, J. ; Varley, M.A. ; Kemp, B. - \ 2003
    Nottingham, UK : Nottingham University Press (Proceedings of previous Easter Schools in agricultural science ) - ISBN 9781897676196 - 536
    varkens - dierveredeling - genetica - bedrijfsvoering - marketing - vleeskwaliteit - diergezondheid - dierenwelzijn - diervoeding - diervoedering - voer - voortplanting - pigs - animal breeding - genetics - management - marketing - meat quality - animal health - animal welfare - animal nutrition - animal feeding - feeds - reproduction
    Perspectives in Pig Science features a collection of papers on five key areas: breeding and genetics; management, marketing and meat quality; health and welfare; reproduction and reproductive management; nutrition.
    Supplementation of plasma with olive oil phenols and extracts: Influence on LDL oxidation
    Leenen, R. ; Roodenburg, A.J.C. ; Vissers, M.N. ; Schuurbiers, J.A.E. ; Putte, K.P.A.M. van; Wiseman, S.A. ; Put, F.H.M.M. van de - \ 2002
    Journal of Agricultural and Food Chemistry 50 (2002)5. - ISSN 0021-8561 - p. 1290 - 1297.
    low-density-lipoprotein - in-vitro oxidation - lipid-peroxidation - heart-disease - vitamin-e - dietary oils - antioxidants - oleuropein - humans - susceptibility
    Phenols present in olive oil may contribute to the health effects of the Mediterranean lifestyle. Olive oil antioxidants increase the resistance of low-density lipoproteins (LDL) against oxidation in vitro, but human intervention studies have failed to demonstrate similar consistent effects. To better mimic the in vivo situation, plasma was incubated with either individual olive oil phenols or olive oil extracts with different phenolic compositions, and LDL was subsequently isolated and challenged for its resistance to oxidation. The results show that the ortho-dihydroxy phenols (hydroxytyrosol and oleuropein-aglycone) are more efficient than their mono-hydroxy counterparts (tyrosol and ligstroside-aglycone) in increasing the resistance of LDL to oxidation. However, the concentration of antioxidants required to inhibit LDL oxidation when added to whole plasma was substantially higher as compared to previous data where antioxidants are directly added to isolated LDL. In conclusion, this study supports the hypothesis that extra virgin olive oil phenols protect LDL in plasma against oxidation. The explanation that in vitro studies show protective effects in contrast to the lack of effect in the majority of human studies may be that the dose of the phenols and thus their plasma concentration in humans was too low to influence ex vivo LDL oxidizability. Further studies are required to gain a better understanding of the potential health benefits that extra virgin olive oil may provide
    Extra virgin olive oil phenols and markers of oxidation in Greek smokers: a randomized cross-over study
    Moschandreas, J. ; Vissers, M.N. ; Wiseman, S. ; Putte, K.P. van; Kafatos, A. - \ 2002
    European Journal of Clinical Nutrition 56 (2002)10. - ISSN 0954-3007 - p. 1024 - 1029.
    low-density-lipoprotein - lipid-peroxidation - cigarette-smoking - plasma - susceptibility - humans - diet
    Objective: To examine the effect of a low phenol olive oil and high phenol olive oil on markers of oxidation and plasma susceptibility to oxidation in normolipaemic smokers. Design: Randomized single-blind cross-over trial with two intervention periods. Setting: The Medical School and University Hospital of the University of Crete, Heraklion, Crete, Greece. Subjects: Twenty-five healthy males and females completed the study. Interventions: Each intervention was of three weeks duration and intervention periods were separated by a two week washout. Seventy grams of extra virgin olive oil was supplied to each subject per day in the intervention periods. The olive oils supplied differed in their phenol content by 18.6 mg/day. Two fasting venous blood samples were taken at the end of each intervention period. Results: The markers of antioxidant capacity measured in fasting plasma samples (total plasma resistance to oxidation, concentrations of protein carbonyl as a marker of protein oxidation, malondialdehyde and lipid hydroperoxides as markers of lipid oxidation and the ferric reducing ability of plasma) did not differ significantly between the low and high phenol olive oil diets. Conclusions: No effect of olive oil phenols on markers of oxidation in smokers was detected. It may be that the natural concentrations of phenols in olive oil are too low to produce an effect in the post-absorptive phase. Possible reasons for period effects and interactions between diet and administration period need attention to aid further cross-over trials of this kind
    Feed composition and environmental pollution
    Verstegen, M.W.A. ; Tamminga, S. - \ 2002
    In: Recent advances in animal nutrition / Gainsworthy, P.C., Wiseman, J., Nottingham University Press - p. 45 - 66.
    Effect of phenol-rich extra virgin olive oil on markers of oxidation in healthy volunteers
    Vissers, M.N. ; Zock, P.L. ; Wiseman, S.A. ; Meyboom, S. ; Katan, M.B. - \ 2001
    European Journal of Clinical Nutrition 55 (2001). - ISSN 0954-3007 - p. 334 - 341.
    Objective: We studied whether consumption of phenol-rich extra virgin olive oil affects the susceptibility of low density lipoproteins (LDL) to oxidation and other markers of oxidation in humans. Design: Randomized cross-over intervention trial, stratified according to sex, age and energy intake. Setting: Division of Human Nutrition and Epidemiology, Wageningen University, The Netherlands. Subjects: Forty-six healthy men and women completed the study. Intervention: Subjects consumed two diets supplying 69 g per day of extra virgin olive oil either rich or poor in phenols for 3 weeks each. The mean difference in phenol intake between the treatments was 18 mg per day. Vitamin E intake was low during the whole study. Fasting blood samples were taken twice at the end of each period. Results: Resistance of LDL and high density lipoprotein (HDL) to oxidation was not affected by treatment. The mean lag time of copper-induced formation of conjugated dienes was 1.6 min shorter in LDL and 0.4 min longer in HDL after the high phenol diet. Other markers of antioxidant capacity in plasma were also not affected: mean lipid hydroperoxides were 0.07 μmol/1 higher, mean malondialdehydes were 0.001 μmol/1 higher, mean protein carbonyls were 0.001 nmol/mg protein lower, and the mean ferric reducing ability of plasma (FRAP) was 0.006 mmol/1 higher after the high phenol diet. All 95␌onfidence intervals enclosed zero. Serum cholesterol concentrations were not affected by the treatment. Conclusion: Consumption of 18 mg per day of phenols from extra virgin olive oil for 3 weeks did not affect LDL or HDL oxidation or other markers of antioxidant capacity in fasting plasma samples.
    Effect of phenols from extra virgin olive oil on markers of oxidation in healthy humans
    Vissers, M.N. ; Zock, P.L. ; Leenen, R. ; Roodenburg, A.J.C. ; Wiseman, S.A. ; Katan, M.B. - \ 2000
    In: Proceedings : European Scientific Conference on Bioactive Micronutrients in Mediterranean Diet and Health, Rome 2000 - p. 1952 - 1952.
    Effect of phenol-rich extra virgin olive oil on LDL oxidation in healthy volunteers
    Vissers, M.N. ; Meyboom, S. ; Wiseman, S.A. ; Zock, P.L. ; Katan, M.B. - \ 1999
    Atherosclerosis 144 (1999). - ISSN 0021-9150 - p. 1 - 3.
    Rearing the layer pullet : a multiphasic approach
    Kwakkel, R.P. - \ 1999
    In: Recent Developments in Poultry Nutrition 2 / Wiseman, J., Garnsworthy, P.C., - p. 227 - 249.
    Pollution issues in pig operations and the influence of nutrition, housing and manure handling
    Klooster, C.E. van 't; Peet-Schwering, C.M.C. van der; Aarnink, A.J.A. ; Lenis, N.P. - \ 1998
    In: Progress in Pig Science / Wiseman, J., Nottingham : Nottingham University Press - ISBN 9781897676264 - p. 507 - 518.
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