Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    De-esterification of methyl-esterified oligogalacturonides
    Alebeek, G.J.W.M. van; Zabotina, O. ; Schols, H.A. ; Beldman, G. ; Voragen, A.G.J. - \ 2000
    In: Proceedings 10th International Symposium Plant Polysaccharides 2000 : 10th International Symposium Plant Polysaccharides 2000, Wageningen, 2000 Wageningen : Wageningen University and Research Centre - p. 89 - 89.
    Structural analysis of (methyl-esterified) oligogalacturonides using post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Alebeek, G.J.W.M. van; Zabotina, O. ; Beldman, G. ; Schols, H.A. ; Voragen, A.G.J. - \ 2000
    Journal of Mass Spectrometry 35 (2000). - ISSN 1076-5174 - p. 831 - 840.
    The use of post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the structural analysis of ((partly) methyl-esterified) oligogalacturonides (oligoGalA) is described. The fragmentation behavior of purified (un)saturated oligoGalA (degree of polymerization 3-6), methyl-esterified and methyl-glycosydated oligoGalA was studied. General fragmentation patterns are described and used for the elucidation of the positions of methyl esters on partly methyl-esterified oligoGalA. This technique now permits the determination of the position of methyl esters or other substituents on pectic fragments, helping in understanding the mode of action of pectinolytic enzymes.
    Esterification and glycosydation of oligogalacturonides : examination of the reaction products using MALDI-TOF MS and HPAEC
    Alebeek, G.J.W.M. van; Zabotina, O. ; Beldman, G. ; Schols, H.A. ; Voragen, A.G.J. - \ 2000
    Carbohydrate Polymers 43 (2000). - ISSN 0144-8617 - p. 39 - 46.
    Methyl-esterified and methyl-glycosydated oligogalacturonides (oligoGalA) were produced to be used as substrates for the characterization of pectinolytic enzymes acting on the homogalacturonan backbone. The reactions were monitored with recently developed techniques like high-performance anion-exchange chromatography (HPAEC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) that allow sensitive monitoring of the reactions. MALDI-TOF MS reveals the degree of esterification and or glycosydation. HPAEC at neutral pH separates not only oligogalacturonides with a different degree of esterifcation but seems to separate some isomers of e.g. monomethyl- and dimethyl-triGalA as well. Breakdown products formed by hydrolysis side reactions were revealed and could even be quantified by HPAEC pH 12 analysis. Using these techniques the conditions for each of the reactions were optimized. Esterification was performed best at concentrations of maximal 0.02N sulfuric acid in anhydrous methanol at 4oC. Hardly any glycosydation occurs and the level of hydrolysis of the oligoGalA was less than 5%. Methyl-glycosydation and simultaneous esterification was performed best in 0.1N sulfuric acid in anhydrous methanol at room temperature. HPAEC revealed only a limited hydrolysis (<11%).
    De-esterification of methyl-esterified oligogalacturonides
    Alebeek, G.J.W.M. van; Zabotina, O. ; Schols, H.A. ; Beldman, G. ; Voragen, A.G.J. - \ 1999
    In: Book of abstracts : the 10th international conference and industrial exhibition on gums and stabilisers for the food industry, Wrexham, UK, 5-9 July 1999. Wrexham : The North East Wales Institute, Plas Coch College, 1999 - p. 21 - 21.
    De-esterification of methyl-esterified oligogalacturonides
    Alebeek, G.J.W.M. van; Zabotina, O. ; Schols, H.A. ; Beldman, G. - \ 1999
    In: 3rd Carbohydrate Bioengineering Meeting, Newcastele upon Tyne, 11-14 April 1999. - University of Newcastle upon Tyne, 1999 - p. 6.14 - 6.14.
    Xyloglucan endotransglycosylase activity in apples is ripening-related: implications for fruit juice processing.
    Vincken, J.P. ; Zabotina, O.A. ; Beldman, G. ; Voragen, A.G.J. - \ 1998
    Journal of the Science of Food and Agriculture 78 (1998). - ISSN 0022-5142 - p. 46 - 52.
    Alterations in cell walls of winter wheat roots during low temperature acclimation.
    Zabotin, A.I. ; Barisheva, T.S. ; Zabotina, O.A. ; Larskaya, I.A. ; Lozovaya, V.V. ; Beldman, G. ; Voragen, A.G.J. - \ 1998
    Journal of Plant Physiology 152 (1998). - ISSN 0176-1617 - p. 473 - 479.
    A possible role for endogenous enzymes in apple liquefaction.
    Vincken, J.P. ; Zabotina, O. ; Broek, B. van den; Beldman, G. ; Voragen, A.G.J. - \ 1996
    In: The Extracellular Matrix of Plants, Keystone Symp., Tamarron, USA - p. 21 - 21.
    Oligosaccharides appeared during first hours of low temperature acclimation increase wheat seedling capability of adaptation.
    Zabotina, O. ; Ayupova, D. ; Larskaya, A. ; Zabotin, A. ; Lozovaya, V.V. ; Beldman, G. ; Voragen, A.G.J. - \ 1996
    In: The Extracellular Matrix of Plants, Keystone Symp., Tamarron, USA - p. 32 - 32.
    Bioactive fragments from pea pectin.
    Zabotina, O. ; Ibragimova, N. ; Ayupova, D. ; Gurjanov, O. ; Lozovaja, V. ; Beldman, G. ; Voragen, A.G.J. - \ 1996
    In: Proc. Int. Symp. on Progress in Biotechnology 14: Pectins and pectinases, Wageningen, 1995, J. Visser, A.G.J. Voragen (eds.). Elsevier Amsterdam, NL - p. 693 - 701.
    Participation of oligosaccharides in the process of root development.
    Zabotina, O. ; Gurjanov, O.P. ; Ayupova, D. ; Ibragimova, N. ; Beldman, G. ; Voragen, A.G.J. ; Lozovaya, V.V. - \ 1996
    In: Abstract 10th Congr. of FISPP, Florence, Italy (1996)
    Incorporation of cell wall of winter roots into the process of low temperature acclimation.
    Zabotin, A. ; Barishe, T.A. ; Zabotina, O. ; Lozovaya, V.V. ; Beldman, G. ; Voragen, A.G.J. - \ 1996
    In: Int. congr. on Stress and inorganic nitrogen assimilation, Moscow - p. 125 - 125.
    Biologically-active soluble oligosaccharides from pea stem tissues.
    Zabotina, O.A. ; Gurjanov, O.P. ; Ayupova, D.A. ; Beldman, G. ; Voragen, A.G.J. ; Lozovaya, V.V. - \ 1996
    Plant Cell Reports 15 (1996). - ISSN 0721-7714 - p. 954 - 957.
    Bioactive oligosaccharides from pea shoots.
    Zabotina, O. ; Gurjanov, O. ; Ayupova, D. ; Beldman, G. ; Voragen, A.G.J. ; Lozovaya, V.V. - \ 1995
    In: Abstract 7th Cell Wall Meeting, Santiago de Compostella, Spain - p. 62 - 62.
    Bioactive fragments from pea pectin.
    Zabotina, O.A. ; Ayupova, D.A. ; Gurjanov, O.P. ; Ibragimova, N. ; Beldman, G. ; Voragen, A.G.J. ; Lozovaya, V.V. - \ 1995
    In: Pectins and Pectinases, Wageningen - p. P30 - P30.
    The isolation and biological activity of oligosaccharins from the pea shoots.
    Zabotina, O.A. ; Gur'yanov, O.P. ; Malikhov, R.G. ; Ayupova, D.A. ; Beldman, G. ; Voragen, A.G.J. ; Lozovaya, V.V. - \ 1995
    Russian Journal of Plant Physiology 42 (1995). - ISSN 1021-4437 - p. 366 - 371.
    Cell wall composition of photosynthetic cultured cells.
    Lozovaya, V.V. ; Widholm, J.M. ; Zabotina, O.A. ; Gorshkova, T.A. ; Kozbevnikov, A. ; Beldman, G. ; Voragen, A.G.J. - \ 1994
    In: Abstract 8th Int. Congr. Plant tissue and cell culture. Firenze - p. 231 - 231.
    Pectic cell wall fragments have influence on buckwheat thin cell lager explant rhizogenesis.
    Zabotina, O. ; Malykov, R. ; Schols, H. ; Beldman, G. ; Lozavaya, V. - \ 1994
    In: Abstract 8th Bratislava Symp. Saccharides, Surdenice, Slovakia - p. 96 - 96.
    Separation of oligosaccharides occurred naturally in plant tissues.
    Zabotina, O. ; Gurjanov, O. ; Agerpova, D. ; Larskaya, I. ; Beldman, G. ; Lozavaya, V. - \ 1994
    In: Abstract 8th Bratislava Symp. Saccharides, Surdenice, Slovakia - p. 95 - 95.
    Alteration in cell walls at low temperature acclimation.
    Zabotin, A.I. ; Bariskova, T. ; Zabotina, O.A. ; Larskaya, I.A. ; Pivovarov, M. ; Beldman, G. ; Lozovaya, V. - \ 1994
    In: Abstract 8th Bratislava Symp. Saccharides, Surdenice, Slovakia - p. 94 - 94.
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