Adventitious plantlet regeneration from floral stem explants of Nerine bowdenii W. Watts.

Abstract

Explants (1.5 mm thick) from the floral stems of cv. Favoriet were cultured on a basal medium (containing Murashige and Skoog micro-elements, half-strength macro-elements and 4% sucrose) with 1 mg/litre BA and 0.5 mg/litre NAA, at 18 degrees C in continuous darkness. Bulblet regeneration was strongly influenced by the age (length) of the original floral stem, optimal regeneration occurring with immature stems 2.5 to 10 cm in length. To obtain plantlets the regenerated bulblets were subcultured on a cytokinin-free medium (with macroelements increased to full strength) at 25 degrees in continuous light. Plantlets were easily transplanted into soil. (Abstract retrieved from CAB Abstracts by CABI’s permission)